def designPrimers(seq_args, global_args=None, misprime_lib=None,
mishyb_lib=None):
''' Run the Primer3 design process.
If the global args have been previously set (either by a pervious
`designPrimers` call or by a `setGlobals` call), `designPrimers` may be
called with seqArgs alone (as a means of optimization).
Args:
seq_args (dict) : Primer3 sequence/design args as per Primer3 docs
Kwargs:
global_args (dict) : Primer3 global args as per Primer3 docs
misprime_lib (dict) : `Sequence name: sequence` dictionary for
mispriming checks.
mishyb_lib (dict) : `Sequence name: sequence` dictionary for
mishybridization checks.
Returns:
A dictionary of Primer3 results (should be identical to the expected
BoulderIO output from primer3_main)
'''
if global_args:
_primer3.setGlobals(global_args, misprime_lib, mishyb_lib)
_primer3.setGlobals(global_args, misprime_lib, mishyb_lib)
_primer3.setSeqArgs(seq_args)
return _primer3.runDesign()
def setP3Globals(global_args, misprime_lib=None, mishyb_lib=None):
''' Set the Primer3 global args and misprime/mishyb libraries.
Args:
global_args (dict) : Primer3 global parameters as per Primer3 docs
Kwargs:
misprime_lib (dict) : `Sequence name: sequence` dictionary for
mispriming checks.
mishyb_lib (dict) : `Sequence name: sequence` dictionary for
mishybridization checks.
'''
_primer3.setGlobals(global_args, misprime_lib, mishyb_lib)
