Analysis of genotypic data of 84 chiropteran residents of an urban tree-roosting colony. All individuals were genotyped at 11 microsatelite loci.
All analyses were conducted in python using jupyter notebook. Follow the link below to examine the notebook using NBviewer.
All but three markers were in linkage equilibrium as assessed with FSTAT v. 2.9.3.2 (Goudet et al., 2001).
Pairwise and mean relatedness values (R) were estimated using ML-Relate (Kalinowski et al. 2006), which implements a corrected maximum-likelihood method for estimating relatedness that allows loci with null alleles to be incorporated into the analysis (Wagner et al. 2006).
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Kalinowski ST, Wagner AP, Taper ML. 2006. Ml-Relate: a Computer Program for Maximum Likelihood Estimation of Relatedness and Relationship. Mol Ecol Notes 6: 576–579. http://doi.wiley.com/10.1111/j.1471-8286.2006.01256.x (Accessed July 15, 2014).
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Wagner AP, Creel S, Kalinowski ST. 2006. Estimating relatedness and relationships using microsatellite loci with null alleles. Heredity (Edinb) 97: 336–45. http://www.ncbi.nlm.nih.gov/pubmed/16868566 (Accessed July 13, 2014).
