• Yin He, Transcriptic
• Ben Miles, Transcriptic
• Alex Carlin, University of California, Davis

To run the protocol, first install the Autoprotocol package and the Transcriptic command line utility, and a preview of the protocol. You must be in the repo working directory for this to work.

pip install autoprotocol transcriptic
transcriptic preview Kunkel 

If you create a new version of the protocol:

transcriptic build-release Kunkel 
transcriptic upload-release Kunkel <my_project_name>

(see transcriptic --help for more). Then, follow the link to publish the protocol on Transcriptic's website.

Then, you can launch runs: Launch a Run > Organization > Kunkel. The run requires you enter

1. an aliquot of ssDNA
2. antibiotic resistance
3. CSV of the mutants you wish to order (see make_csv.py in this repo)