This deep mutational scan of human adenosine deaminase is in the early planning phase. Currently

• have synthesized a gene and cloned into vector
• expressed and purified, and optimized purification, in Escherichia
• developed low-cost, high-throughput manual assay
• developed low-cost, high-throughput automated assay for Transcriptic (now defunct)

Next steps

• computational deep mutation scan
• feature generation
• machine learning
• error prone PCR protocol
• nicking/Kunkel/NNK oligo design and protocol
• Neat sequencing