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Internal web app for Kunkels

Goal

My goal is to build an app that we can deploy that automates the following tasks:

  • the creation of a set of oligos that make a set of mutants. The app will have to be given the coding nucleotide sequence, and a wild type PDB structure.
  • the ordering of those mutants via Kunkel from Transcriptic via the Kunkel API
  • it will then create a sequencing order (since we can't use Transcriptic sequencing since it is too expensive)
  • it will then ship the plate (TS API)
  • [how can it possibly submit an order to Genscript?]
  • submit a order to GenScript
  • retrieve sequencing results
  • algorithmically compare those results to the wild type sequence
  • collect all the mutations in a given clone into a list
  • compare that list against the list that was ordered
    • [submit the mutants that were not found through the whole thing again]
  • generate a map that gives the well in which to find each mutant
  • print this map out and give to a human

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Internal web app for Kunkels

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