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Tormenta

Measurement control and analysis for optical microscopy

image

Installation

Ubuntu

Run in terminal:

```
$ sudo apt-get install python3-pip python3-h5py git
$ sudo pip3 install comtypes lantz 

$ sudo apt-get install build-essential python3-dev cython3 python3-setuptools python3-pip python3-wheel python3-numpy   python3-pytest python3-blosc python3-brotli python3-snappy python3-lz4 libz-dev libblosc-dev liblzma-dev liblz4-dev libzstd-dev libpng-dev libwebp-dev libbz2-dev libopenjp2-7-dev libjpeg-turbo8-dev libjxr-dev liblcms2-dev libcharls-dev libaec-dev libbrotli-dev libsnappy-dev libzopfli-dev libgif-dev libtiff-dev

$ pip3 install imagecodecs
$ pip3 install tifffile pyqtgraph
$ git clone https://github.com/fedebarabas/
```

Windows

  • Install WinPython 3.4.
  • Browse to Laboratory for Fluorescence Dynamics and download tifffile for Python 3.4 to $PATH\WinPython-64bit-3.4.4.1\python-3.4.4.amd64\.
  • Open WinPython Command Prompt and run:

    $ pip install comtypes lantz tifffile-2016.4.19-cp34-cp34m-win_amd64.whl
  • Clone Repository by running:

` $ git clone https://github.com/marcnol/tormenta.git`

Optional dependencies

Don't install these libraries if you have different equipment or you just want to test the software without instruments (offline mode). -Support for Labjack's T7 DAQ - LJM Library - LJM Library Python wrapper -Support for webcam image acquisition - Pygame

Launch Tormenta

  • Open WinPython Command Prompt, go to tormenta's repository directory and run:

    $ python -m tormenta

Documentation

The documentation is under construction in http://fedebarabas.github.io/tormenta/

How to cite

If you used the code/program for your paper, please cite

Barabas et al., Note: Tormenta: An open source Python-powered control software for camera based optical microscopy, Review of Scientific Instruments, 2016.

https://doi.org/10.1063/1.4972392

Contact

Feel free to contact me with comments or suggestions. Use any part of the code that suits your needs.

Federico Barabas fede.barabas[AT]gmail.com

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Measurement control and analysis for super-resolution localization fluorescence microscopy

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