Version: 1.0

Release date: 08 Aug 2014

Website: http://fenderglass.github.io/Ragout/

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Ragout (Reference-Assisted Genome Ordering UTility) is a tool for assisted assembly using multiple references. It takes a short read assembly (a set of contigs), a set of related references and a corresponding phylogenetic tree and then assembles the contigs into scaffolds.

The benefits of assembly with multiple references become significant, when those references have structural variations compared to the target genome. Even if each reference is structurally divergent, it is possible to assemble the target into the correct set of scaffolds. Enlarge your contigs with Ragout!

The current version of Ragout was mostly tested on bacterial genomes, however it also contains an experimental support of mamalian-scaled ones.

See docs/INSTALL.md file.

See docs/USAGE.md file.

• Mikhail Kolmogorov (St. Petersburg University of the Russian Academy of Sciences)
• Pavel Avdeev (St. Petersburg University of the Russian Academy of Sciences)
• Dmitriy Meleshko (St. Petersburg University of the Russian Academy of Sciences)
• Son Pham (University of California, San Diego)

• Mikhail Kolmogorov, Brian Raney, Benedict Paten, and Son Pham. "Ragout: A reference-assisted assembly tool for bacterial genomes", Bioinformatics, 2014

Supplementary materials for ISMB submission could be found at: https://drive.google.com/file/d/0B1pUguR1yn7TMjNpX09JdFphT3c/edit?usp=sharing

Please report any bugs directly to the issue tracker of this project. Also, you can send your feedback at fenderglass@gmail.com

The work was partially supported by VP Foundation.

We would like to thank:

• Nikolay Vyahhi (testing and some useful suggestions)
• Aleksey Gurevich (testing)

Ragout includes some third-patry software (see INSTALL.md for details)

• Networkx Python library [http://networkx.github.io/]
• Newick Python parser [http://www.daimi.au.dk/~mailund/newick.html]
• Sibelia [http://github.com/bioinf/Sibelia]

Ragout itself is distributed under BSD license, but the package also contains some third-party software. Most of this software is completely free to redistribute, but some such as Sibelia or Newick parser are released under the GPL. We therefore release Ragout distribution under the GPL and note that the licenses of the constituent packages can be viewed in their subfolders. (see LICENSE file)