def filter(self, dataframe=None, store=True):
# Retrieve the dataframe
if dataframe is None:
dataframe = self.spots
# Connect positions together
dataframe = tp.link(
dataframe,
self.search_range,
memory=self.memory,
adaptive_stop=self.adaptive_stop,
adaptive_step=self.adaptive_step,
neighbor_strategy=self.neighbor_strategy,
link_strategy=self.link_strategy,
)
# Remove spurious trajectory
if self.filter_stubs is not None:
dataframe = tp.filtering.filter_stubs(dataframe,
threshold=self.filter_stubs)
# Regenerate the index
dataframe = dataframe.reset_index(drop=True)
# Store in the instance
if store:
self.tracks = deepcopy(dataframe)
return dataframe
def workerFunc(locData: Optional[pd.DataFrame], searchRange: float,
memory: int) -> Union[pd.DataFrame, None]:
"""Perform tracking
Parameters
----------
locData
Localization data for tracking
searchRange
`search_range` parameter to :py:func:`trackpy.link`
memory
`memory` parameter to :py:func:`trackpy.link`
Returns
-------
Tracked data
"""
if locData is None:
return None
if not locData.size:
ret = locData.copy()
ret["particle"] = []
return ret
trackpy.quiet()
return trackpy.link(locData, search_range=searchRange, memory=memory)
def time_series_analysis(particles, max_dist=1, memory=3, properties=['area']):
"""
Perform tracking of particles for times series data.
Parameters
----------
particles : Particle_list object.
max_dist : int
The maximum distance between the same particle in subsequent images.
memory : int
The number of frames to remember particles over.
properties : list
A list of particle properties to track over the time series.
Returns
-------
Pandas DataFrame of tracjectories.
"""
df = pd.DataFrame(columns=['y', 'x'] + properties + ['frame'])
for particle in particles.list:
pd_dict = {
'x': particle.properties['x']['value'],
'y': particle.properties['y']['value']
}
for property in properties:
pd_dict.update({property: particle.properties[property]['value']})
pd_dict.update({'frame': particle.properties['frame']['value']})
df = df.append([pd_dict])
t = trackpy.link(df, max_dist, memory=memory)
return (t)
def test_link_memory(self):
expected = pd.DataFrame(self.coords_link,
columns=self.pos_columns + ['frame'])
expected['frame'] = expected['frame'].astype(np.int)
actual = tp.link(expected, memory=self.memory, **self.link_params)
expected['particle'] = self.expected_link_memory
assert_traj_equal(actual, expected)
def test_link_memory(self):
expected = pd.DataFrame(self.coords_link,
columns=self.pos_columns + ['frame'])
expected['frame'] = expected['frame'].astype(np.int)
actual = tp.link(expected, memory=self.memory, **self.link_params)
expected['particle'] = self.expected_link_memory
assert_traj_equal(actual, expected)
def localize(video, method='tf', background=None, dark_count=31):
'''
Returns DataFrame of particle parameters in each frame
of a video linked with their trajectory index
Args:
video: video filename
Keywords:
background: background image for normalization
dark_count: dark count of camera
'''
if method == 'tf':
trk = tracker.tracker()
# Create VideoCapture to read video
cap = cv2.VideoCapture(video)
# Initialize components to build overall dataset.
frame_no = 0
data = []
while(cap.isOpened()):
ret, image = cap.read()
if ret is False:
break
image = cv2.cvtColor(image, cv2.COLOR_BGR2GRAY)
# Normalize image
if background is not None:
image = (image - dark_count) / (background - dark_count)
# Find features in each frame
if method == 'tf':
features = trk.predict(inflate(image))
elif method == 'oat':
features, circ = oat(image, frame_no)
else:
raise(ValueError("method must be either \'oat\' or \'tf\'"))
for feature in features:
# Build dataset over all frames.
feature = np.append(feature, frame_no)
data.append(feature)
# Advance frame_no
frame_no += 1
cap.release()
# Put data set in DataFrame and link
result_df = pd.DataFrame(columns=['x', 'y', 'w', 'h', 'frame'], data=data)
linked_df = tp.link(result_df, search_range=5, pos_columns=['y', 'x'])
return linked_df
def link(self, search_range: int = 5, memory: int = 2, **configs):
# Make sure particles have been located
if self.locations is None:
print(' ! No locations found.')
return
configs['search_range'] = search_range
configs['memory'] = memory
# self.link_config has the highest priority
configs.update(self.link_configs)
# Link locations
self.trajectories = tp.link(self.locations, **configs)
console.show_status('Linking completed. Configurations:')
console.supplement(configs)
self.effective_link_config = configs
self._draw()
def get_link(locs, search_range, memory):
'''
Apply trackpy.link_df() (`trackpy`_) on localizations with given search_range and memory to get trajectories sorted by group and frame.
All tracks shorter or equal to 10 frames are removed.
Args:
locs(pandas.DataFrame): Localizations as generated by `picasso.localize`_ as pandas.DataFrame
info(picasso.io): Info _locs.yaml to _locs.hdf5 localizations as list of dictionaries.
search_range(int): Localizations within search_range (spatial) will be connected to tracks (see trackpy.link_df)
memory(int): Localizations within memory (temporal) will be connected to tracks (see trackpy.link_df)
Return:
pandas.DataFrame:
Linked trajectories using trackpy.link().
- ``group`` instead of ``particle`` column for `picasso.render`_ compatibility.
- All trajectories with <= 10 localizations are already removed!!
'''
### Link locs
link = tp.link(
locs,
search_range,
memory=memory,
link_strategy='hybrid',
)
### Sort and rename
print('Sorting by [group,frame] ... ')
link.sort_values(by=['particle', 'frame'], ascending=True, inplace=True)
link = link.rename(
columns={'particle':
'group'}) # Rename to groups for picasso compatibility
### Throw away tracks with only 10 localizations
link = drop_shorttracks(link)
return link
def link(self, search_range=15, memory=3):
self.data.df = tp.link(self.data.df.reset_index(),
search_range,
memory=memory).set_index('frame')
self.data.save()
def link(self, *args, **kwargs):
kwargs.update(self.linker_opts)
return tp.link(*args, **kwargs)
import filehandling
from particletracking import dataframes
import trackpy as tp
# direc1 = filehandling.open_directory(initialdir='/media/data/Data')
# files1 = filehandling.get_directory_filenames(direc1+'/*.hdf5')
#
# direc2 = filehandling.open_directory(initialdir='/media/data/Data')
# files2 = filehandling.get_directory_filenames(direc1+'/*.hdf5')
#
# files = files1 + files2
files = ["/media/data/Data/FirstOrder/PhaseDiagram/FlatPlate2Feb2021/2000.hdf5",]
for file in files:
with dataframes.DataStore(file) as data:
d = data.df.copy()
print(d.head())
d = d.reset_index()
print(d.head())
d = tp.link(d, d.r.mean())
def link(self, *args, **kwargs):
kwargs.update(self.linker_opts)
return tp.link(*args, **kwargs)
link_distance = tracking_setting['link_distance']
memory = tracking_setting['memory']
adaptive_step = tracking_setting['adaptive_step']
adaptive_stop = tracking_setting['adaptive_stop']
df_data_tracked = pd.DataFrame()
for pos in pos_list:
print("tracking nuclei for Pos %d...." % int(pos))
df_select = df_data.loc[df_data['Pos'] == pos].copy().reset_index(
drop=True)
df_select = tp.link(df_select,
link_distance,
pos_columns=['cent_x', 'cent_y'],
t_column='frame',
memory=memory,
adaptive_step=adaptive_step,
adaptive_stop=adaptive_stop)
df_select = df_select.rename(columns={'particle': 'ID'})
df_select = df_select.sort_values(['frame', 'ID'])
df_select['ID'] = df_select['ID'] + 1 # to make it start from 1
df_data_tracked = pd.concat((df_data_tracked, df_select))
print("Done!\n")
df_data = df_data_tracked.reset_index(drop=True)
del df_data_tracked
del df_select
path = r'C:\Users\elpresidente_2\Desktop\MatlabPA14\Particle Tracking\cropped_movie'
frames = pims.open(path + r'\*.tif')
print(type(frames))
proc_frames = []
# First detect particles in each frame
for frame in frames:
frame_ = cv2.GaussianBlur(frame, (5, 5), 0)
frame = cv2.adaptiveThreshold(frame_, 255, cv2.ADAPTIVE_THRESH_GAUSSIAN_C,
cv2.THRESH_BINARY, 9, 2)
proc_frames.append(frame)
loc = tp.batch(proc_frames, 13, invert=True,
minmass=2000) # Found minmass from histogram
t = tp.link(loc, 9, memory=4)
t1 = tp.filter_stubs(t, 20)
plt.figure()
tp.plot_traj(t1)
plt.show()
#tp.annotate(f, frames[0])
#fig, ax = plt.subplots()
#ax.hist(f['mass'], bins=20)
#ax.set_xlabel('mass')
#ax.set_ylabel('count')
#plt.show()
#f = tp.locate(th3, 13, invert=True, minmass = 2000)
#tp.annotate(f, frame)
dfn = os.path.join(output_dir, 'tracking.pickle')
if not os.path.exists(dfn):
print("Tracking points...")
import trackpy as tp
# Open the 3D movie
vol = open_3D_movie(mfn)
# Identify the points in the volume
data = tp.batch(vol, 5)
# Note: the second parameter is the size in the frame. In this case,
# 5 is approximately right, but you would need to tweak for a different
# data set!
# Link the particles into tracks
data = tp.link(data, 10, memory=3)
print(f'Found {data.particle.max()+1} particle tracks')
# Find the physical coordinates. These will appear as the columns "xc", "yc",
# and "zc" in the data frame *after* you run this command, and will be in the
# physical units of the data (i.e. L and not N)
vol.distortion.update_data_frame(data)
# Save to a pickle file
with open(dfn, "wb") as f:
pickle.dump(data, f)
else:
print(f"Found existing track data, delete {dfn} to regenerate...")
with open(dfn, "rb") as f:
data = pickle.load(f)
plt.figure()
plot_blobs(substack[i][:, :, 2], blobs)
plt.savefig(f'img_w_blobs_{i}.png')
plt.close()
#%%
all_blob_data = []
for i, each in tqdm.tqdm(enumerate(allblobs)):
blob_locs = pd.DataFrame(data={'x': [], 'y': [], 'frame': []})
blob_locs['x'] = each[:, 1]
blob_locs['y'] = each[:, 0]
blob_locs['frame'] = i
all_blob_data.append(blob_locs)
allblob_data = pd.concat(all_blob_data).reset_index(drop=True)
#%% Link blob blocations into tracks
linked = tp.link(allblob_data, search_range=30, memory=5)
filt_linked = tp.filter_stubs(linked)
by_pid = filt_linked.groupby('particle')
#%%
# Remove all tracks which are suspiciously stationary
def startend_travelled(track_df):
if track_df.shape[0] < 2:
return 0
start_pos = track_df.loc[np.min(track_df.index), ['x', 'y']]
end_pos = track_df.loc[np.max(track_df.index), ['x', 'y']]
dist = np.sqrt(np.sum((end_pos - start_pos)**2))
return dist
@author: MattiaV
"""
from MTM import matchTemplates
import cv2
from skimage import io
import matplotlib.pyplot as plt
from template_opt import Template90
from track import make_batch
import trackpy as tp
fileID = r"C:\Users\MattiaV\Desktop\università\Interships\DynamicsOfGranularShapes\ParticleTracking\Images\First frames\*.png"
images = io.imread_collection(fileID)
temp_draft1 = images[0][320:433,520:650]
temp0 = temp_draft1[23:92,28:105]
Temp = Template90(temp0)
list_template = Temp.create()
features_match = {'N_obj':50, 'threshold':0.55, 'method':cv2.TM_CCOEFF_NORMED, 'max_overlap':0.22}
batch = make_batch(images, list_template, features_match)
t0 = tp.link(batch, 50, memory = 3)
t1 = tp.filter_stubs(t0, 4)
# Compare the number of particles in the unfiltered and filtered data.
print('Before:', t0['particle'].nunique())
print('After:', t1['particle'].nunique())
plt.figure()
tp.plot_traj(t1);
v = pims.ImageSequence(impath)
# take reader that provides uint8!
assert np.issubdtype(v.dtype, np.uint8)
v0 = tp.invert_image(v[0])
v0_bp = tp.bandpass(v0, lshort=1, llong=9)
expected_find = tp.grey_dilation(v0, separation=9)
expected_find_bandpass = tp.grey_dilation(v0_bp, separation=9)
expected_refine = tp.refine_com(v0, v0_bp, radius=4,
coords=expected_find_bandpass)
expected_refine = expected_refine[expected_refine['mass'] >= 140]
expected_refine_coords = expected_refine[pos_columns].values
expected_locate = tp.locate(v0, diameter=9, minmass=140)
expected_locate_coords = expected_locate[pos_columns].values
df = tp.locate(v0, diameter=9)
df = df[(df['x'] < 64) & (df['y'] < 64)]
expected_characterize = df[pos_columns + char_columns].values
f = tp.batch(tp.invert_image(v), 9, minmass=140)
f_crop = f[(f['x'] < 320) & (f['x'] > 280) & (f['y'] < 280) & (f['x'] > 240)]
f_linked = tp.link(f_crop, search_range=5, memory=0)
f_linked_memory = tp.link(f_crop, search_range=5, memory=2)
link_coords = f_linked[pos_columns + ['frame']].values
expected_linked = f_linked['particle'].values
expected_linked_memory = f_linked_memory['particle'].values
np.savez_compressed(npzpath, expected_find, expected_find_bandpass,
expected_refine_coords, expected_locate_coords,
link_coords, expected_linked, expected_linked_memory,
expected_characterize)
def analyze(self, plot_gif=False):
self.drifts = []
self.v_drift_mag = []
self.D_constants = []
self.D_constants2 = []
self.msd_slope = []
self.msd_intercept = []
self.mu_hats = []
self.ed = []
self.em = []
self.frames = []
self.dataframes = []
for i, path in enumerate(self.SXM_PATH):
frames = SXMReader(path)
self.frames.append(frames)
self.NM_PER_PIXEL = frames.meters_per_pixel * 1e9
molecule_size, min_mass, max_mass, separation, min_size, max_ecc, adaptive_stop, search_range, _ = self.PARAMS[
i]
f = tp.batch(frames,
molecule_size,
minmass=min_mass,
separation=separation)
t = tp.link(f,
search_range=search_range,
adaptive_stop=adaptive_stop)
t1 = t[((t['mass'] > min_mass) & (t['size'] > min_size)
& (t['ecc'] < max_ecc)) & (t['mass'] < max_mass)]
t2 = tp.filter_stubs(t, 3)
# Compare the number of particles in the unfiltered and filtered data.
print('Before:', t['particle'].nunique())
print('After:', t2['particle'].nunique())
if plot_gif == True:
moviename = "{}-{}".format(min(self.fileranges[i]),
max(self.fileranges[i]))
singlemoviefolder = self.MOVIE_FOLDER + moviename + "/"
if not os.path.exists(singlemoviefolder):
os.makedirs(singlemoviefolder)
mpl.rcParams.update({'font.size': 14, 'font.weight': 'bold'})
mpl.rc('image', origin='lower')
mpl.rc('text', usetex=False)
mpl.rc('text', color='orange')
fns = []
for j, frame in enumerate(frames):
fig = plt.figure(figsize=(5, 5))
tp.plot_traj(t2[(t2['frame'] <= j)],
superimpose=frames[j],
label=True)
fn = singlemoviefolder + "Image_{}.png".format(
self.fileranges[i][j])
fig.savefig(fn)
fns.append(fn)
ax = plt.gca() # get the axis
ax.set_ylim(ax.get_ylim()[::-1]) # invert the axis
ax.xaxis.tick_top() # and move the X-Axis
ax.yaxis.set_ticks(np.arange(0, 16, 1)) # set y-ticks
ax.yaxis.tick_left() # remove right y-Ticks
plt.clf()
mpl.rc('text', color='black')
images = []
for fn in fns:
images.append(imageio.imread(fn))
imageio.mimsave(singlemoviefolder + moviename + '.gif',
images,
duration=0.5)
self._cleanup_png(singlemoviefolder)
# Compute drifts
d = tp.compute_drift(t2)
d.loc[0] = [0, 0]
t3 = t2.copy()
# Storing drifts
self.drifts.append(d)
# Method 1 of calculating D: variance of all displacements of Delta_t=1
displacements = self._calculate_displacements(t3)
self.D_constants.append(
(displacements.dx.var() + displacements.dy.var()) /
4) # r^2 = x^2 + y^2 = 2Dt + 2Dt
self.mu_hats.append(np.mean(displacements[['dx', 'dy']], axis=0))
# Method 2 of calculating D: linear fit to MSD
em = tp.emsd(t3,
frames.meters_per_pixel * 1e9,
self.DIFFUSION_TIME,
max_lagtime=len(frames),
detail=True)
self.em.append(em)
self.ed.append([em['<x>'], em['<y>']])
result = linregress(em.index[:-8] * self.DIFFUSION_TIME,
em['msd'][:-8])
self.msd_slope.append(result.slope)
self.msd_intercept.append(result.intercept)
self.D_constants2.append(result.slope / 4)
# Store dataframe for future analysis
self.dataframes.append(t3)
self.v_drift_mag = np.linalg.norm(self.mu_hats, 2, axis=1)
def linking_trackpy(features,
field_in,
dt,
dxy,
v_max=None,
d_max=None,
d_min=None,
subnetwork_size=None,
memory=0,
stubs=1,
time_cell_min=None,
order=1,
extrapolate=0,
method_linking='random',
adaptive_step=None,
adaptive_stop=None,
cell_number_start=1):
"""
Function to perform the linking of features in trajectories
Parameters:
features: pandas.DataFrame
Detected features to be linked
v_max: float
speed at which features are allowed to move
dt: float
time resolution of tracked features
dxy: float
grid spacing of input data
memory int
number of output timesteps features allowed to vanish for to be still considered tracked
subnetwork_size int
maximim size of subnetwork for linking
method_detection: str('trackpy' or 'threshold')
flag choosing method used for feature detection
method_linking: str('predict' or 'random')
flag choosing method used for trajectory linking
"""
# from trackpy import link_df
import trackpy as tp
from copy import deepcopy
# from trackpy import filter_stubs
# from .utils import add_coordinates
# calculate search range based on timestep and grid spacing
if v_max is not None:
search_range = int(dt * v_max / dxy)
# calculate search range based on timestep and grid spacing
if d_max is not None:
search_range = int(d_max / dxy)
# calculate search range based on timestep and grid spacing
if d_min is not None:
search_range = max(search_range, int(d_min / dxy))
if time_cell_min:
stubs = np.floor(time_cell_min / dt) + 1
logging.debug('stubs: ' + str(stubs))
logging.debug('start linking features into trajectories')
#If subnetwork size given, set maximum subnet size
if subnetwork_size is not None:
tp.linking.Linker.MAX_SUB_NET_SIZE = subnetwork_size
# deep copy to preserve features field:
features_linking = deepcopy(features)
if method_linking is 'random':
# link features into trajectories:
trajectories_unfiltered = tp.link(features_linking,
search_range=search_range,
memory=memory,
t_column='frame',
pos_columns=['hdim_2', 'hdim_1'],
adaptive_step=adaptive_step,
adaptive_stop=adaptive_stop,
neighbor_strategy='KDTree',
link_strategy='auto')
elif method_linking is 'predict':
pred = tp.predict.NearestVelocityPredict(span=1)
trajectories_unfiltered = pred.link_df(
features_linking,
search_range=search_range,
memory=memory,
pos_columns=['hdim_1', 'hdim_2'],
t_column='frame',
neighbor_strategy='KDTree',
link_strategy='auto',
adaptive_step=adaptive_step,
adaptive_stop=adaptive_stop
# copy_features=False, diagnostics=False,
# hash_size=None, box_size=None, verify_integrity=True,
# retain_index=False
)
else:
raise ValueError('method_linking unknown')
# Filter trajectories to exclude short trajectories that are likely to be spurious
# trajectories_filtered = filter_stubs(trajectories_unfiltered,threshold=stubs)
# trajectories_filtered=trajectories_filtered.reset_index(drop=True)
# Reset particle numbers from the arbitray numbers at the end of the feature detection and linking to consecutive cell numbers
# keep 'particle' for reference to the feature detection step.
trajectories_unfiltered['cell'] = None
for i_particle, particle in enumerate(
pd.Series.unique(trajectories_unfiltered['particle'])):
cell = int(i_particle + cell_number_start)
trajectories_unfiltered.loc[trajectories_unfiltered['particle'] ==
particle, 'cell'] = cell
trajectories_unfiltered.drop(columns=['particle'], inplace=True)
trajectories_bycell = trajectories_unfiltered.groupby('cell')
for cell, trajectories_cell in trajectories_bycell:
logging.debug("cell: " + str(cell))
logging.debug("feature: " + str(trajectories_cell['feature'].values))
logging.debug("trajectories_cell.shape[0]: " +
str(trajectories_cell.shape[0]))
if trajectories_cell.shape[0] < stubs:
logging.debug("cell" + str(cell) + " is a stub (" +
str(trajectories_cell.shape[0]) +
"), setting cell number to Nan..")
trajectories_unfiltered.loc[trajectories_unfiltered['cell'] ==
cell, 'cell'] = np.nan
trajectories_filtered = trajectories_unfiltered
#Interpolate to fill the gaps in the trajectories (left from allowing memory in the linking)
trajectories_filtered_unfilled = deepcopy(trajectories_filtered)
# trajectories_filtered_filled=fill_gaps(trajectories_filtered_unfilled,order=order,
# extrapolate=extrapolate,frame_max=field_in.shape[0]-1,
# hdim_1_max=field_in.shape[1],hdim_2_max=field_in.shape[2])
# add coorinates from input fields to output trajectories (time,dimensions)
# logging.debug('start adding coordinates to trajectories')
# trajectories_filtered_filled=add_coordinates(trajectories_filtered_filled,field_in)
# add time coordinate relative to cell initiation:
# logging.debug('start adding cell time to trajectories')
trajectories_filtered_filled = trajectories_filtered_unfilled
trajectories_final = add_cell_time(trajectories_filtered_filled)
# add coordinate to raw features identified:
logging.debug('start adding coordinates to detected features')
logging.debug('feature linking completed')
return trajectories_final
def Link(searchRange, memory, minFrames):
t = tp.link(f, search_range = searchRange, memory = memory)
t1 = tp.filter_stubs(t, minFrames)
plt.figure()
tp.plot_traj(t1, label = True)
return t1
# take reader that provides uint8!
assert np.issubdtype(v.dtype, np.uint8)
v0 = tp.invert_image(v[0])
v0_bp = tp.bandpass(v0, lshort=1, llong=9)
expected_find = tp.grey_dilation(v0, separation=9)
expected_find_bandpass = tp.grey_dilation(v0_bp, separation=9)
expected_refine = tp.refine_com(v0,
v0_bp,
radius=4,
coords=expected_find_bandpass)
expected_refine = expected_refine[expected_refine['mass'] >= 140]
expected_refine_coords = expected_refine[pos_columns].values
expected_locate = tp.locate(v0, diameter=9, minmass=140)
expected_locate_coords = expected_locate[pos_columns].values
df = tp.locate(v0, diameter=9)
df = df[(df['x'] < 64) & (df['y'] < 64)]
expected_characterize = df[pos_columns + char_columns].values
f = tp.batch(tp.invert_image(v), 9, minmass=140)
f_crop = f[(f['x'] < 320) & (f['x'] > 280) & (f['y'] < 280) & (f['x'] > 240)]
f_linked = tp.link(f_crop, search_range=5, memory=0)
f_linked_memory = tp.link(f_crop, search_range=5, memory=2)
link_coords = f_linked[pos_columns + ['frame']].values
expected_linked = f_linked['particle'].values
expected_linked_memory = f_linked_memory['particle'].values
np.savez_compressed(npzpath, expected_find, expected_find_bandpass,
expected_refine_coords, expected_locate_coords,
link_coords, expected_linked, expected_linked_memory,
expected_characterize)
median = np.median(temp, axis=2)
@pims.pipeline
def removeBack(frame, median):
frame = frame - median
return frame
frames = removeBack(frames, median)
break
#frames = cropImage(frames)
#fig.savefig(SavePath + '/Line.jpg')
f = tp.batch(frames, estimateFeatureSize, minmass=minMass)
t = tp.link(f, 60, memory=80)
t1 = tp.filter_stubs(t, 10)
tp.plot_traj(t1)
Ntrajs = np.max(np.array(t1['particle'])) + 1
minMoveDistance = 1
print('there are %s trajectories' % Ntrajs)
t2 = t1[0:0]
for i in range(Ntrajs):
tNew = t1[t1['particle'] == i]
if (len(tNew) < 30):
continue
#distData = tp.motion.msd(tNew,1,1,len(tNew))
#dist = distData.iloc[-1,:]['msd']
def link(self, *args, **kwargs):
kwargs.update(self.linker_opts)
return tp.link(args[0], validate_tuple(args[1], 2),
*args[2:], **kwargs)
def link(self, *args, **kwargs):
kwargs.update(self.linker_opts)
return tp.link(args[0], validate_tuple(args[1], 2), *args[2:],
**kwargs)
