Pyhon code for the analysis of confocal laser-scanning microscopy based fluorescence fluctuation spectroscopy (FFS) data. The code is designed to analyze FFS data obtained with a 5x5 pixel SPAD array detector, instead of the typical point-detector. Includes code for:

• reading raw data files
• calculating autocorrelations and cross-correlations
• fitting the correlation curves with various fit models

Under Examples, a Jupyter notebook is available with an example. The raw data set used here is available on . This data set contains the fluorescence intensity time traces for the 25 pixels for a freely diffusing antibody conjugated Alexa 488 dye. The time traces can be analyzed in various ways, such as spot-variation FCS, two-focus or pair-correlation FCS, and intensity mean-squared-displacement analysis.