Mostly we want to work with non-scaffold based origami, so exporting all sequences, including that of what Cadnano thinks is a scaffold is pretty important... have made the change to accomodate this.

Cadnano is computer-aided design software for DNA origami nanostructures. The original citation is here.

This version of Cadnano2 is being maintained by the Douglas Lab to preserve the lattice-based design interface for research purposes. This is not a direct fork of cadnano/cadnano2 because that would result in a 200+ MB download when cloning. We have removed the installer directory and git history, which makes this version less than 3 MB to download, or 11 MB uncompressed.

If you wish to use the newest version of Cadnano that supports Python scripting and non-lattice designs, see cadnano2.5.

OS X

• Install homebrew
• Install python3: brew install python3
• Optional: Set up a virtualenv (recommended)
• Install dependencies: pip3 install PyQt5 networkx
• Clone git repo: git clone https://github.com/douglaslab/cadnano2

Windows

• Download and install latest python3
• Optional: Set up a virtualenv (recommended)
• pip3 install PyQt5 networkx

Linux

• Optional: Set up a virtualenv (recommended)
• Install dependencies: pip3 install PyQt5 networkx
• Clone git repo: git clone https://github.com/douglaslab/cadnano2

• Open the terminal and navigate to the path of the cadnano2 git repo.
• python3 main.py

Some environment variables for debugging or customization:

• CADNANO_DISCARD_UNSAVED: Don't prompt the user to save unsaved changes; just exit.
• CADNANO_DEFAULT_DOCUMENT On creation of the default document, open the named file (put a path to the file in the value of the environment variable) instead of a blank document.
• CADNANO_IGNORE_ENV_VARS_EXCEPT_FOR_ME: Turn off other cadnano environment variables.

This version of Cadnano2 is available under the MIT License. GUI code that uses PyQt5 or PyQt3D is GPLv3 as required by Riverbank Computing.