This python script measures the quantification of the formation of spots around a certain pixel size (by default 2.07 pixels), returns a value for each time frame of the image stack.

1. Download the deltaM folder. ("Download or Clone" the project)
2. Copy the deltaM folder to the plugins folder inside Fiji. You may need to relaunch Fiji.

1. Open the image stack. Some sample image stacks are provided in my SharePoint account
2. Locate and run the script at the bottom of Plugins menu.
3. The maximum intensity projection of the image will be displayed, the measures will pop up in a plot. Values may be exported selecting the option "Save".

NOTE: Running it in Windows, will display a warning : "console: Failed to install '': java.nio.charset.UnsupportedCharsetException: cp0."

It is a known issue for Jython, adding -Dpython.console.encoding=UTF-8 as a VM argument to the run configuration for my program. For example, run from console as:

imageJ-win64.exe -Dpython.console.encoding=UTF-8

This plugin runs in a modified version of the LoG3D plugin, that can be found in website of Daniel Sage