Draws the FRAPPA region on iQ images. This is especially useful for activation or stimulation experiments to see the targeted region. A copy of the image is created in the ImageList in which the FRAPPA ROI outline is drawn destructively onto the image by setting the pixel intensity to a high value.

This plugin is for presentation purposes. The FRAP analysis ImageJ plugin reliably guesses the FRAP location without the ROI metadata, and is recommended for quantitative work.

1. Download a release and unzip in the plugins directory. Developers may instead want to clone this Git respository into the plugins directory.

2. Plugins directory for iQ 2.5.0 and above: C:/Program Files/Andor Bioimaging/PythonEngine/Plugins/

   For iQ 2.4.4 and earlier: C:/Program Files/Andor Bioimaging/Plugins/

3. (For iQ 2.5.0) The PIL module is broken, so first delete the folder C:\iQOpenSource\Python-2.6.6\Lib\site-packages\PIL-1.1.7-py2.6-win32 and install PIL 1.17 for Python 2.6

4. Now when you restart / start the Python IDE you will always see this program in the plugins menu.

5. To test, in iQ open a dataset from the data/ folder and rename it simply to demo_image