System for controlling ZP lab microscopes from python code. The system has a few moving parts (literally and figuratively), so here's an introduction. Currently Leica DM6000 and DMi8 are supported, with Andor Zyla cameras.

(1) The microscope-driving code runs as a server. Users and GUIs connect to the "scope daemon" and send it commands, over a simple RPC protocol. (JSON transmitted over ZeroMQ sockets.) These connections can be either local or over the network.

(2) In addition to the command-driven RPC connections, the server also publishes updates about changes to the state of the microscope that clients can subscribe to. This is useful for both GUIs and for getting updates about things like when a new image is available in live-viewing mode.

(3) The server transfers images to local clients via named shared memory areas, which enables fast, zero-copy transfers of large quantities of image data. Remote clients instead get data serialized over the RPC connection.

(4) On the server-side, the microscope itself is represented as a "Scope" object with sub-objects referring to various hardware components attached, including the camera, temperature controller, etc.

(5) The Leica scope itself has a fairly rich/complex serial API that involves sending and receiving commands, possibly in asynchronous fashion. So there is a set of message-manager classes that mind the serial port in a background thread and take care of sending messages to the serial port and calling the appropriate callbacks when messages are received. Any microscope components can be set to "synchronous" mode where functions don't return until the microscope is done executing them, or "async_" mode where all functions return immediately, except wait() which will block until all previous outstanding functions are done executing.

(6) The Andor camera is also rather complex, and like the Leica scope, is effectively a state machine. Lowlevel wrappers for the Andor C API are auto- generated, and then prettified into a Camera object that encapsulates most of the complexity. Advanced users will likely need to read both the camera's hardware manual and SDK documentation.

(7) Several components are integrated by a custom microcontroller that sends and receives TTL pulses and PWM analog signals. This microcontroller, IOTool, is used to sequence camera acquisitions in synch with light pulses from the transmitted lamp and the Spectra X fluorescence excitation source.

RPC Protocol The RPC client and server code is in simple_rpc/rpc_[client|server].py

Methods of the microscope controller object (and its attributes) are exposed to clients via a simple RPC protocol that consists of JSON messages sent over ZeroMQ request/reply sockets. Why? Several reasons: first, users can connect locally OR remotely, and from Python or any other language they choose if it supports ZeroMQ. Next, this allows any microscope GUIs to be totally decoupled from the "scope daemon" that actually drives the hardware. Finally, multiple clients can connect at once, which seems like a bad idea, but allows one to remotely check up on the microscope state during long data acquisition runs.

In addition to the basic RPC server, an out-of-band "Interrupt Server" is also run over a separate socket, which allows "KeyboardInterrupt" signals to be sent to break out of any RPC command.

The basic RPC protocol is that the client sends a JSON encoding of the command name, its args, and kwargs. The server then looks up the command name, calls the command, and replies in a multi-part ZeroMQ message. The first part specifies if the reply contains error data (which is JSON-serialized, and intended to be raised as an exception on the client side), JSON reply data, or binary reply data. The server can also provide detailed descriptions of all the commands in its namespace, allowing the client to build up a rich set of proxy functions to be called.

Property Protocol The property client and server code is in simple_rpc/property_[client|server].py

In addition to an imperative RPC architecture, there is an asynchronous "property server" that allows clients to be notified of any changes to the state of the microscope or its components. This is mostly useful for GUI programs, but is also used when the camera's "live mode" is on to notify clients when new images are available.

This is a fairly straightforward use of ZeroMQ publish/subscribe sockets, with some cleverness on the client side to allow callbacks to be registered for updates to specific properties, or to all properties with a common prefix. Scope properties are named e.g. scope.stage.x, so common prefixes are very useful.

Interprocess Shared Memory This uses the "ISM_Buffer" library that we wrote: https://github.com/zplab/SharedMemoryBuffer and tools in transfer_ism_buffer.py.

ISM_Buffers are named, shared-memory regions that can be opened by different processes and allow for zero-copy sharing of data between processes. (Moreover, ISM_Buffers can serve as the backing memory for numpy arrays.) To allow this to be more seamless, ISM_Buffers maintain a shared reference count, so when the last python reference to a given ISM_Buffer is deleted, it will tear down the whole ISM_Buffer too, preventing memory leaks.

These buffers must be handed from the server to the client carefully to ensure that the server doesn't acidentally tear down the ISM_Buffer before the client receives it and increments its reference count. This is accomplished as follows:

First, on the server, a named ISM_Buffer is created (in transfer_ism_buffer.py), and a numpy array that is a view onto that shared memory is created. Any function that returns an "image" to the client must first register the ISM_Buffer by name (via transfer_ism_buffer.register_array_for_transfer()) which causes the server to keep a reference to the ISM_Buffer-backed array around. Then the ISM_Buffer's name is returned to the client, which the client can use to create its own ISM_Buffer view onto that memory. Once this is accomplished, the client calls transfer_ism_buffer._server_release_array() to tell the server that it need no longer keep it's own reference.

This is all taken care of by transfer_ism_buffer.client_get_data_getter(), which returns a function called get_data() that, given a ISM_Buffer name, performs all of the above steps. The scope client even monkeypatches things so that all known calls that return an ISM_Buffer name get wrapped with this get_data() function, so that things work transparently.

Network Image Streaming The above is all well and good if the client is local on that machine and can access the shared memory. If the client is remote, then it must ask the server to pack the named ISM_Buffer's memory into binary data and send that over RPC. This is also transparently handled by transfer_ism_buffer.client_get_data_getter(), which will detect if the client and server are not on the same machine, and return a get_data() function that causes network data transfer to occur.

Message-Based Devices (Leica Scope) The relevant code is messaging/message_[device|manager].py

For the Leica microscope, a MessageManager controls access to a serial port in a background thread. Messages are queued up to be sent over, with callback functions to be called when a reply to that given message is received. (Replies and callbacks are matched up via a "key" generated based on the reply contents.)

Each class that represents a part of the microscope is a subclass of message_device.AsyncDevice. This base class interacts with a MessageManager to send and receive messages either in an async_ mode (where seveal messages can be sent before calling wait() to block until all have been replied to) or in synchronous mode where sending a message blocks until the reply for that message is received.