def mergebed(args):
"""
%prog mergebed map1.bed map2.bed map3.bed ...
Combine bed maps to bed format, adding the map name.
"""
p = OptionParser(mergebed.__doc__)
p.add_option("-w",
"--weightsfile",
default="weights.txt",
help="Write weights to file")
p.set_outfile("out.bed")
opts, args = p.parse_args(args)
if len(args) < 1:
sys.exit(not p.print_help())
maps = args
outfile = opts.outfile
fp = must_open(maps)
b = Bed()
mapnames = set()
for row in fp:
mapname = fp.filename().split(".")[0]
mapnames.add(mapname)
try:
m = BedLine(row)
m.accn = "{0}-{1}".format(mapname, m.accn)
m.extra = ["{0}:{1}".format(m.seqid, m.start)]
b.append(m)
except (IndexError, ValueError): # header or mal-formed line
continue
b.print_to_file(filename=outfile, sorted=True)
logging.debug("A total of {0} markers written to `{1}`.".\
format(len(b), outfile))
assert len(maps) == len(mapnames), "You have a collision in map names"
write_weightsfile(mapnames, weightsfile=opts.weightsfile)
def mergebed(args):
"""
%prog mergebed map1.bed map2.bed map3.bed ...
Combine bed maps to bed format, adding the map name.
"""
p = OptionParser(mergebed.__doc__)
p.add_option("-w", "--weightsfile", default="weights.txt",
help="Write weights to file")
p.set_outfile("out.bed")
opts, args = p.parse_args(args)
if len(args) < 1:
sys.exit(not p.print_help())
maps = args
outfile = opts.outfile
fp = must_open(maps)
b = Bed()
mapnames = set()
for row in fp:
mapname = fp.filename().split(".")[0]
mapnames.add(mapname)
try:
m = BedLine(row)
m.accn = "{0}-{1}".format(mapname, m.accn)
m.extra = ["{0}:{1}".format(m.seqid, m.start)]
b.append(m)
except (IndexError, ValueError): # header or mal-formed line
continue
b.print_to_file(filename=outfile, sorted=True)
logging.debug("A total of {0} markers written to `{1}`.".\
format(len(b), outfile))
assert len(maps) == len(mapnames), "You have a collision in map names"
write_weightsfile(mapnames, weightsfile=opts.weightsfile)
def shuffle_twobeds(afbed, bfbed, bbfasta, prefix=None):
# Shuffle the two bedfiles together
sz = Sizes(bbfasta)
sizes = sz.mapping
shuffled = "shuffled.bed"
border = bfbed.order
all = []
afbed.sort(key=afbed.nullkey)
totalids = len(sizes)
pad = int(math.log10(totalids)) + 1
cj = 0
seen = set()
accn = lambda x: "{0}{1:0{2}d}".format(prefix, x, pad)
for seqid, aa in afbed.sub_beds():
cj += 1
abeds, bbeds, beds = [], [], []
size = sizes[seqid]
ranges = [(x.seqid, x.start, x.end) for x in aa]
cranges = range_interleave(ranges, sizes={seqid: size}, empty=True)
for crange in cranges:
if crange:
seqid, start, end = crange
bedline = "\t".join(str(x) for x in (seqid, start - 1, end))
abeds.append(BedLine(bedline))
else:
abeds.append(None)
for a in aa:
gapid = a.accn
bi, b = border[gapid]
if a.strand == "-":
b.extra[1] = b.strand = "-" if b.strand == "+" else "+"
bbeds.append(b)
n_abeds = len(abeds)
n_bbeds = len(bbeds)
assert n_abeds - n_bbeds == 1, "abeds: {0}, bbeds: {1}".format(n_abeds, n_bbeds)
beds = [x for x in roundrobin(abeds, bbeds) if x]
if prefix:
for b in beds:
b.accn = accn(cj)
all.extend(beds)
seen.add(seqid)
# Singletons
for seqid, size in sz.iter_sizes():
if seqid in seen:
continue
bedline = "\t".join(str(x) for x in (seqid, 0, size, accn(cj)))
b = BedLine(bedline)
cj += 1
if prefix:
b.accn = accn(cj)
all.append(b)
shuffledbed = Bed()
shuffledbed.extend(all)
shuffledbed.print_to_file(shuffled)
return shuffledbed
def shuffle_twobeds(afbed, bfbed, bbfasta, prefix=None):
# Shuffle the two bedfiles together
sz = Sizes(bbfasta)
sizes = sz.mapping
shuffled = "shuffled.bed"
border = bfbed.order
all = []
afbed.sort(key=afbed.nullkey)
totalids = len(sizes)
pad = int(math.log10(totalids)) + 1
cj = 0
seen = set()
accn = lambda x: "{0}{1:0{2}d}".format(prefix, x, pad)
for seqid, aa in afbed.sub_beds():
cj += 1
abeds, bbeds, beds = [], [], []
size = sizes[seqid]
ranges = [(x.seqid, x.start, x.end) for x in aa]
cranges = range_interleave(ranges, sizes={seqid: size}, empty=True)
for crange in cranges:
if crange:
seqid, start, end = crange
bedline = "\t".join(str(x) for x in (seqid, start - 1, end))
abeds.append(BedLine(bedline))
else:
abeds.append(None)
for a in aa:
gapid = a.accn
bi, b = border[gapid]
if a.strand == '-':
b.extra[1] = b.strand = ('-' if b.strand == '+' else '+')
bbeds.append(b)
n_abeds = len(abeds)
n_bbeds = len(bbeds)
assert n_abeds - n_bbeds == 1, \
"abeds: {0}, bbeds: {1}".format(n_abeds, n_bbeds)
beds = [x for x in roundrobin(abeds, bbeds) if x]
if prefix:
for b in beds:
b.accn = accn(cj)
all.extend(beds)
seen.add(seqid)
# Singletons
for seqid, size in sz.iter_sizes():
if seqid in seen:
continue
bedline = "\t".join(str(x) for x in (seqid, 0, size, accn(cj)))
b = BedLine(bedline)
cj += 1
if prefix:
b.accn = accn(cj)
all.append(b)
shuffledbed = Bed()
shuffledbed.extend(all)
shuffledbed.print_to_file(shuffled)
return shuffledbed
def install(args):
"""
%prog install patchers.bed patchers.fasta backbone.fasta alt.fasta
Install patches into backbone, using sequences from alternative assembly.
The patches sequences are generated via jcvi.assembly.patch.fill().
The output is a bedfile that can be converted to AGP using
jcvi.formats.agp.frombed().
"""
from jcvi.apps.base import blast
from jcvi.formats.blast import BlastSlow
from jcvi.formats.fasta import SeqIO
from jcvi.utils.iter import roundrobin
p = OptionParser(install.__doc__)
p.add_option(
"--rclip",
default=1,
type="int",
help="Pair ID is derived from rstrip N chars [default: %default]")
p.add_option(
"--maxsize",
default=1000000,
type="int",
help="Maximum size of patchers to be replaced [default: %default]")
p.add_option("--prefix",
help="Prefix of the new object [default: %default]")
p.add_option(
"--strict",
default=False,
action="store_true",
help="Only update if replacement has no gaps [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 4:
sys.exit(not p.print_help())
pbed, pfasta, bbfasta, altfasta = args
Max = opts.maxsize # Max DNA size to replace gap
rclip = opts.rclip
prefix = opts.prefix
blastfile = blast([altfasta, pfasta, "--wordsize=100", "--pctid=99"])
order = Bed(pbed).order
beforebed, afterbed = "before.bed", "after.bed"
fwa = open(beforebed, "w")
fwb = open(afterbed, "w")
key1 = lambda x: x.query
key2 = lambda x: x.query[:-rclip] if rclip else key1
data = BlastSlow(blastfile)
for pe, lines in groupby(data, key=key2):
lines = list(lines)
if len(lines) != 2:
continue
a, b = lines
aquery, bquery = a.query, b.query
asubject, bsubject = a.subject, b.subject
if asubject != bsubject:
continue
astrand, bstrand = a.orientation, b.orientation
assert aquery[-1] == 'L' and bquery[-1] == 'R', str((aquery, bquery))
ai, ax = order[aquery]
bi, bx = order[bquery]
qstart, qstop = ax.start + a.qstart - 1, bx.start + b.qstop - 1
if astrand == '+' and bstrand == '+':
sstart, sstop = a.sstart, b.sstop
elif astrand == '-' and bstrand == '-':
sstart, sstop = b.sstart, a.sstop
else:
continue
if sstart > sstop:
continue
if sstop > sstart + Max:
continue
name = aquery[:-1] + "LR"
print >> fwa, "\t".join(str(x) for x in \
(ax.seqid, qstart - 1, qstop, name, 1000, "+"))
print >> fwb, "\t".join(str(x) for x in \
(asubject, sstart - 1, sstop, name, 1000, astrand))
fwa.close()
fwb.close()
beforefasta = fastaFromBed(beforebed, bbfasta, name=True, stranded=True)
afterfasta = fastaFromBed(afterbed, altfasta, name=True, stranded=True)
# Exclude the replacements that contain more Ns than before
ah = SeqIO.parse(beforefasta, "fasta")
bh = SeqIO.parse(afterfasta, "fasta")
count_Ns = lambda x: x.seq.count('n') + x.seq.count('N')
exclude = set()
for arec, brec in zip(ah, bh):
an = count_Ns(arec)
bn = count_Ns(brec)
if opts.strict:
if bn == 0:
continue
elif bn < an:
continue
id = arec.id
exclude.add(id)
logging.debug("Ignore {0} updates because of decreasing quality."\
.format(len(exclude)))
abed = Bed(beforebed, sorted=False)
bbed = Bed(afterbed, sorted=False)
abed = [x for x in abed if x.accn not in exclude]
bbed = [x for x in bbed if x.accn not in exclude]
abedfile = "before.filtered.bed"
bbedfile = "after.filtered.bed"
afbed = Bed()
afbed.extend(abed)
bfbed = Bed()
bfbed.extend(bbed)
afbed.print_to_file(abedfile)
bfbed.print_to_file(bbedfile)
# Shuffle the two bedfiles together
sz = Sizes(bbfasta)
sizes = sz.mapping
shuffled = "shuffled.bed"
border = bfbed.order
all = []
afbed.sort(key=afbed.nullkey)
totalids = len(sizes)
import math
pad = int(math.log10(totalids)) + 1
cj = 0
seen = set()
accn = lambda x: "{0}{1:0{2}d}".format(prefix, x, pad)
for seqid, aa in afbed.sub_beds():
cj += 1
abeds, bbeds, beds = [], [], []
size = sizes[seqid]
ranges = [(x.seqid, x.start, x.end) for x in aa]
cranges = range_interleave(ranges, sizes={seqid: size})
for seqid, start, end in cranges:
bedline = "\t".join(str(x) for x in (seqid, start - 1, end))
abeds.append(BedLine(bedline))
for a in aa:
gapid = a.accn
bi, b = border[gapid]
bbeds.append(b)
a = abeds[0] if abeds else []
assert abs(len(abeds) - len(bbeds)) <= 1
if (not a) or a.start > 1:
abeds, bbeds = bbeds, abeds
beds = list(roundrobin(abeds, bbeds))
if prefix:
for b in beds:
b.accn = accn(cj)
all.extend(beds)
seen.add(seqid)
# Singletons
for seqid, size in sz.iter_sizes():
if seqid in seen:
continue
bedline = "\t".join(str(x) for x in (seqid, 0, size, accn(cj)))
b = BedLine(bedline)
cj += 1
if prefix:
b.accn = accn(cj)
all.append(b)
shuffledbed = Bed()
shuffledbed.extend(all)
shuffledbed.print_to_file(shuffled)
