def gaps(args):
"""
%prog gaps OM.bed fastafile
Create patches around OM gaps.
"""
from jcvi.formats.bed import uniq
p = OptionParser(gaps.__doc__)
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
ombed, fastafile = args
ombed = uniq([ombed])
bed = Bed(ombed)
for a, b in pairwise(bed):
om_a = (a.seqid, a.start, a.end, "+")
om_b = (b.seqid, b.start, b.end, "+")
ch_a = range_parse(a.accn)
ch_b = range_parse(b.accn)
ch_a = (ch_a.seqid, ch_a.start, ch_a.end, "+")
ch_b = (ch_b.seqid, ch_b.start, ch_b.end, "+")
om_dist, x = range_distance(om_a, om_b, distmode="ee")
ch_dist, x = range_distance(ch_a, ch_b, distmode="ee")
if om_dist <= 0 and ch_dist <= 0:
continue
print(a)
print(b)
print(om_dist, ch_dist)
def distance(args):
"""
%prog distance bedfile
Calculate distance between bed features. The output file is a list of
distances, which can be used to plot histogram, etc.
"""
from jcvi.utils.iter import pairwise
p = OptionParser(distance.__doc__)
p.add_option("--distmode", default="ss", choices=("ss", "ee"),
help="Distance mode between paired reads. ss is outer distance, " \
"ee is inner distance [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(not p.print_help())
bedfile, = args
sortedbedfile = sort([bedfile])
valid = total = 0
fp = open(sortedbedfile)
for a, b in pairwise(fp):
a = BedLine(a)
b = BedLine(b)
ar = (a.seqid, a.start, a.end, "+")
br = (b.seqid, b.start, b.end, "+")
dist, oo = range_distance(ar, br, distmode=opts.distmode)
total += 1
if dist > 0:
print dist
valid += 1
logging.debug("Total valid (> 0) distances: {0}.".\
format(percentage(valid, total)))
def distance(args):
"""
%prog distance bedfile
Calculate distance between bed features. The output file is a list of
distances, which can be used to plot histogram, etc.
"""
from jcvi.utils.iter import pairwise
p = OptionParser(distance.__doc__)
p.add_option("--distmode", default="ss", choices=("ss", "ee"),
help="Distance mode between paired reads. ss is outer distance, " \
"ee is inner distance [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(not p.print_help())
bedfile, = args
sortedbedfile = sort([bedfile])
valid = total = 0
fp = open(sortedbedfile)
for a, b in pairwise(fp):
a = BedLine(a)
b = BedLine(b)
ar = (a.seqid, a.start, a.end, "+")
br = (b.seqid, b.start, b.end, "+")
dist, oo = range_distance(ar, br, distmode=opts.distmode)
total += 1
if dist > 0:
print dist
valid += 1
logging.debug("Total valid (> 0) distances: {0}.".\
format(percentage(valid, total)))
def fromblast(args):
"""
%prog fromblast blastfile subject.fasta
Generate path from BLAST file. If multiple subjects map to the same query,
an edge is constructed between them (with the link provided by the query).
The BLAST file MUST be filtered, chained, supermapped.
"""
from jcvi.formats.blast import sort
from jcvi.utils.range import range_distance
p = OptionParser(fromblast.__doc__)
p.add_option(
"--clique",
default=False,
action="store_true",
help="Populate clique instead of linear path",
)
p.add_option(
"--maxdist",
default=100000,
type="int",
help="Create edge within certain distance",
)
p.set_verbose(help="Print verbose reports to stdout")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
blastfile, subjectfasta = args
clique = opts.clique
maxdist = opts.maxdist
sort([blastfile, "--query"])
blast = BlastSlow(blastfile, sorted=True)
g = BiGraph()
for query, blines in groupby(blast, key=lambda x: x.query):
blines = list(blines)
iterator = combinations(blines, 2) if clique else pairwise(blines)
for a, b in iterator:
asub, bsub = a.subject, b.subject
if asub == bsub:
continue
arange = (a.query, a.qstart, a.qstop, "+")
brange = (b.query, b.qstart, b.qstop, "+")
dist, oo = range_distance(arange, brange, distmode="ee")
if dist > maxdist:
continue
atag = ">" if a.orientation == "+" else "<"
btag = ">" if b.orientation == "+" else "<"
g.add_edge(asub, bsub, atag, btag)
graph_to_agp(g, blastfile, subjectfasta, verbose=opts.verbose)
def fromblast(args):
"""
%prog fromblast blastfile subject.fasta
Generate path from BLAST file. If multiple subjects map to the same query,
an edge is constructed between them (with the link provided by the query).
The BLAST file MUST be filtered, chained, supermapped.
"""
from jcvi.formats.blast import sort
from jcvi.utils.range import range_distance
p = OptionParser(fromblast.__doc__)
p.add_option("--clique", default=False, action="store_true",
help="Populate clique instead of linear path [default: %default]")
p.add_option("--maxdist", default=100000, type="int",
help="Create edge within certain distance [default: %default]")
p.add_option("--verbose", default=False, action="store_true",
help="Print verbose reports to stdout [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
blastfile, subjectfasta = args
clique = opts.clique
maxdist = opts.maxdist
sort([blastfile, "--query"])
blast = BlastSlow(blastfile, sorted=True)
g = BiGraph()
for query, blines in groupby(blast, key=lambda x: x.query):
blines = list(blines)
iterator = combinations(blines, 2) if clique else pairwise(blines)
for a, b in iterator:
asub, bsub = a.subject, b.subject
if asub == bsub:
continue
arange = (a.query, a.qstart, a.qstop, "+")
brange = (b.query, b.qstart, b.qstop, "+")
dist, oo = range_distance(arange, brange, distmode="ee")
if dist > maxdist:
continue
atag = ">" if a.orientation == "+" else "<"
btag = ">" if b.orientation == "+" else "<"
g.add_edge(BiEdge(asub, bsub, atag, btag))
graph_to_agp(g, blastfile, subjectfasta, verbose=opts.verbose)
def get_distance(a, b, xaxis=True):
"""
Returns the distance between two blast HSPs.
"""
if xaxis:
arange = ("0", a.qstart, a.qstop, a.orientation) # 0 is the dummy chromosome
brange = ("0", b.qstart, b.qstop, b.orientation)
else:
arange = ("0", a.sstart, a.sstop, a.orientation)
brange = ("0", b.sstart, b.sstop, b.orientation)
dist, oo = range_distance(arange, brange, distmode="ee")
dist = abs(dist)
return dist
def get_distance(a, b, xaxis=True):
"""
Returns the distance between two blast HSPs.
"""
if xaxis:
arange = ("0", a.qstart, a.qstop, a.orientation) # 0 is the dummy chromosome
brange = ("0", b.qstart, b.qstop, b.orientation)
else:
arange = ("0", a.sstart, a.sstop, a.orientation)
brange = ("0", b.sstart, b.sstop, b.orientation)
dist, oo = range_distance(arange, brange, distmode="ee")
dist = abs(dist)
return dist
def gaps(args):
"""
%prog gaps OM.bed fastafile
Create patches around OM gaps.
"""
from jcvi.formats.bed import uniq
from jcvi.utils.iter import pairwise
p = OptionParser(gaps.__doc__)
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
ombed, fastafile = args
ombed = uniq([ombed])
bed = Bed(ombed)
for a, b in pairwise(bed):
om_a = (a.seqid, a.start, a.end, "+")
om_b = (b.seqid, b.start, b.end, "+")
ch_a = range_parse(a.accn)
ch_b = range_parse(b.accn)
ch_a = (ch_a.seqid, ch_a.start, ch_a.end, "+")
ch_b = (ch_b.seqid, ch_b.start, ch_b.end, "+")
om_dist, x = range_distance(om_a, om_b, distmode="ee")
ch_dist, x = range_distance(ch_a, ch_b, distmode="ee")
if om_dist <= 0 and ch_dist <= 0:
continue
print a
print b
print om_dist, ch_dist
def estimate_size(accns, bed, order, conservative=True):
"""
Estimate the bp length for the deletion tracks, indicated by the gene accns.
True different levels of estimates vary on conservativeness.
"""
accns = [order[x] for x in accns]
ii, bb = zip(*accns)
mini, maxi = min(ii), max(ii)
if not conservative: # extend one gene
mini -= 1
maxi += 1
minb = bed[mini]
maxb = bed[maxi]
assert minb.seqid == maxb.seqid
distmode = "ss" if conservative else "ee"
ra = (minb.seqid, minb.start, minb.end, "+")
rb = (maxb.seqid, maxb.start, maxb.end, "+")
dist, orientation = range_distance(ra, rb, distmode=distmode)
assert dist != -1
return dist
def estimate_size(accns, bed, order, conservative=True):
"""
Estimate the bp length for the deletion tracks, indicated by the gene accns.
True different levels of estimates vary on conservativeness.
"""
accns = [order[x] for x in accns]
ii, bb = zip(*accns)
mini, maxi = min(ii), max(ii)
if not conservative: # extend one gene
mini -= 1
maxi += 1
minb = bed[mini]
maxb = bed[maxi]
assert minb.seqid == maxb.seqid
distmode = "ss" if conservative else "ee"
ra = (minb.seqid, minb.start, minb.end, "+")
rb = (maxb.seqid, maxb.start, maxb.end, "+")
dist, orientation = range_distance(ra, rb, distmode=distmode)
assert dist != -1
return dist
def fastpairs(args):
"""
%prog fastpairs castabfile
Assuming paired reads are adjacent in the castabfile. Print pair distance
and orientations.
"""
from jcvi.utils.range import range_distance
from jcvi.assembly.base import orientationlabels
p = OptionParser(fastpairs.__doc__)
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(p.print_help())
castabfile, = args
fp = open(castabfile)
arow = fp.readline()
while arow:
brow = fp.readline()
a, b = CasTabLine(arow), CasTabLine(brow)
asubject, astart, astop = a.refnum, a.refstart, a.refstop
bsubject, bstart, bstop = b.refnum, b.refstart, b.refstop
if -1 not in (astart, bstart):
aquery, bquery = a.readname, b.readname
astrand, bstrand = a.strand, b.strand
dist, orientation = range_distance(\
(asubject, astart, astop, astrand),
(bsubject, bstart, bstop, bstrand)
)
orientation = orientationlabels[orientation]
if dist != -1:
print "\t".join(
str(x) for x in (aquery, bquery, dist, orientation))
arow = fp.readline()
def fastpairs(args):
"""
%prog fastpairs castabfile
Assuming paired reads are adjacent in the castabfile. Print pair distance
and orientations.
"""
from jcvi.utils.range import range_distance
from jcvi.assembly.base import orientationlabels
p = OptionParser(fastpairs.__doc__)
opts, args = p.parse_args(args)
if len(args) != 1:
sys.exit(p.print_help())
castabfile, = args
fp = open(castabfile)
arow = fp.readline()
while arow:
brow = fp.readline()
a, b = CasTabLine(arow), CasTabLine(brow)
asubject, astart, astop = a.refnum, a.refstart, a.refstop
bsubject, bstart, bstop = b.refnum, b.refstart, b.refstop
if -1 not in (astart, bstart):
aquery, bquery = a.readname, b.readname
astrand, bstrand = a.strand, b.strand
dist, orientation = range_distance(\
(asubject, astart, astop, astrand),
(bsubject, bstart, bstop, bstrand)
)
orientation = orientationlabels[orientation]
if dist != -1:
print "\t".join(str(x) for x in (aquery, bquery, dist, orientation))
arow = fp.readline()
def pastegenes(args):
"""
%prog pastegenes coverage.list old.genes.bed new.genes.bed old.assembly
Paste in zero or low coverage genes. For a set of neighboring genes
missing, add the whole cassette as unplaced scaffolds. For singletons the
program will try to make a patch.
"""
from jcvi.formats.base import DictFile
from jcvi.utils.cbook import gene_name
p = OptionParser(pastegenes.__doc__)
p.add_option("--cutoff", default=90, type="int",
help="Coverage cutoff to call gene missing [default: %default]")
p.add_option("--flank", default=2000, type="int",
help="Get the seq of size on two ends [default: %default]")
p.add_option("--maxsize", default=50000, type="int",
help="Maximum size of patchers to be replaced [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 4:
sys.exit(not p.print_help())
coveragefile, oldbed, newbed, oldassembly = args
cutoff = opts.cutoff
flank = opts.flank
maxsize = opts.maxsize
coverage = DictFile(coveragefile, valuepos=2, cast=float)
obed = Bed(oldbed)
order = obed.order
bed = [x for x in obed if x.accn in coverage]
key = lambda x: coverage[x.accn] >= cutoff
extrabed = "extra.bed"
extendbed = "extend.bed"
pastebed = "paste.bed"
fw = open(extrabed, "w")
fwe = open(extendbed, "w")
fwp = open(pastebed, "w")
fw_ids = open(extendbed + ".ids", "w")
singletons, large, large_genes = 0, 0, 0
for chr, chrbed in groupby(bed, key=lambda x: x.seqid):
chrbed = list(chrbed)
for good, beds in groupby(chrbed, key=key):
if good:
continue
beds = list(beds)
blocksize = len(set([gene_name(x.accn) for x in beds]))
if blocksize == 1:
singletons += 1
accn = beds[0].accn
gi, gb = order[accn]
leftb = obed[gi - 1]
rightb = obed[gi + 1]
leftr = leftb.range
rightr = rightb.range
cur = gb.range
distance_to_left, oo = range_distance(leftr, cur)
distance_to_right, oo = range_distance(cur, rightr)
span, oo = range_distance(leftr, rightr)
if distance_to_left <= distance_to_right and \
distance_to_left > 0:
label = "LEFT"
else:
label = "RIGHT"
if 0 < span <= maxsize:
print >> fwp, "\t".join(str(x) for x in \
(chr, leftb.start, rightb.end, gb.accn))
print >> fwe, leftb
print >> fwe, gb
print >> fwe, rightb
print >> fwe, "L:{0} R:{1} [{2}]".format(distance_to_left, \
distance_to_right, label)
print >> fw_ids, gb.accn
continue
large += 1
large_genes += blocksize
ranges = [(x.start, x.end) for x in beds]
rmin, rmax = range_minmax(ranges)
rmin -= flank
rmax += flank
name = "-".join((beds[0].accn, beds[-1].accn))
print >> fw, "\t".join(str(x) for x in (chr, rmin - 1, rmax, name))
fw.close()
fwe.close()
extrabed = mergeBed(extrabed, d=flank, nms=True)
fastaFromBed(extrabed, oldassembly, name=True)
summary([extrabed])
logging.debug("Singleton blocks : {0}".format(singletons))
logging.debug("Large blocks : {0} ({1} genes)".format(large, large_genes))
def test_range_distance(a, b, distmode, expected):
from jcvi.utils.range import range_distance
assert range_distance(a, b, distmode) == expected
def report_pairs(data,
cutoff=0,
mateorientation=None,
pairsfile=None,
insertsfile=None,
rclip=1,
ascii=False,
bins=20,
distmode="ss",
mpcutoff=1000):
"""
This subroutine is used by the pairs function in blast.py and cas.py.
Reports number of fragments and pairs as well as linked pairs
"""
allowed_mateorientations = ("++", "--", "+-", "-+")
if mateorientation:
assert mateorientation in allowed_mateorientations
num_fragments, num_pairs = 0, 0
all_dist = []
linked_dist = []
# +- (forward-backward) is `innie`, -+ (backward-forward) is `outie`
orientations = defaultdict(int)
# clip how many chars from end of the read name to get pair name
key = (lambda x: x.accn[:-rclip]) if rclip else (lambda x: x.accn)
data.sort(key=key)
if pairsfile:
pairsfw = open(pairsfile, "w")
if insertsfile:
insertsfw = open(insertsfile, "w")
for pe, lines in groupby(data, key=key):
lines = list(lines)
if len(lines) != 2:
num_fragments += len(lines)
continue
num_pairs += 1
a, b = lines
asubject, astart, astop = a.seqid, a.start, a.end
bsubject, bstart, bstop = b.seqid, b.start, b.end
aquery, bquery = a.accn, b.accn
astrand, bstrand = a.strand, b.strand
dist, orientation = range_distance(\
(asubject, astart, astop, astrand),
(bsubject, bstart, bstop, bstrand),
distmode=distmode)
if dist >= 0:
all_dist.append((dist, orientation, aquery, bquery))
# select only pairs with certain orientations - e.g. innies, outies, etc.
if mateorientation:
all_dist = [x for x in all_dist if x[1] == mateorientation]
# try to infer cutoff as twice the median until convergence
if cutoff <= 0:
dists = np.array([x[0] for x in all_dist], dtype="int")
p0 = analyze_dists(dists, cutoff=mpcutoff)
cutoff = int(2 * p0) # initial estimate
cutoff = int(math.ceil(cutoff / bins)) * bins
logging.debug("Insert size cutoff set to {0}, ".format(cutoff) +
"use '--cutoff' to override")
for dist, orientation, aquery, bquery in all_dist:
if dist > cutoff:
continue
if cutoff > 2 * mpcutoff and dist < mpcutoff:
continue
linked_dist.append(dist)
if pairsfile:
print >> pairsfw, "{0}\t{1}\t{2}".format(aquery, bquery, dist)
orientations[orientation] += 1
print >>sys.stderr, "{0} fragments, {1} pairs ({2} total)".\
format(num_fragments, num_pairs, num_fragments + num_pairs * 2)
s = SummaryStats(linked_dist, dtype="int")
num_links = s.size
meandist, stdev = s.mean, s.sd
p0, p1, p2 = s.median, s.p1, s.p2
print >>sys.stderr, "%d pairs (%.1f%%) are linked (cutoff=%d)" % \
(num_links, num_links * 100. / num_pairs, cutoff)
print >>sys.stderr, "mean distance between mates: {0} +/- {1}".\
format(meandist, stdev)
print >> sys.stderr, "median distance between mates: {0}".format(p0)
print >> sys.stderr, "95% distance range: {0} - {1}".format(p1, p2)
print >> sys.stderr, "\nOrientations:"
orientation_summary = []
for orientation, count in sorted(orientations.items()):
o = "{0}:{1}".format(orientation, \
percentage(count, num_links, mode=1))
orientation_summary.append(o.split()[0])
print >> sys.stderr, o
if insertsfile:
from jcvi.graphics.histogram import histogram
print >> insertsfw, "\n".join(str(x) for x in linked_dist)
insertsfw.close()
prefix = insertsfile.rsplit(".", 1)[0]
if prefix > 10:
prefix = prefix.split("-")[0]
osummary = " ".join(orientation_summary)
title = "{0} ({1}; median:{2} bp)".format(prefix, osummary, p0)
histogram(insertsfile,
vmin=0,
vmax=cutoff,
bins=bins,
xlabel="Insertsize",
title=title,
ascii=ascii)
if op.exists(insertsfile):
os.remove(insertsfile)
return s
def fromblast(args):
"""
%prog fromblast blastfile subject.fasta
Generate path from BLAST file. If multiple subjects map to the same query,
an edge is constructed between them (with the link provided by the query).
The BLAST file MUST be filtered, chained, supermapped.
"""
from jcvi.formats.blast import sort
from jcvi.utils.range import range_distance
p = OptionParser(fromblast.__doc__)
p.add_option("--clique", default=False, action="store_true",
help="Populate clique instead of linear path [default: %default]")
p.add_option("--maxdist", default=100000, type="int",
help="Create edge within certain distance [default: %default]")
p.add_option("--verbose", default=False, action="store_true",
help="Print verbose reports to stdout [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
blastfile, subjectfasta = args
clique = opts.clique
maxdist = opts.maxdist
sort([blastfile, "--query"])
blast = BlastSlow(blastfile, sorted=True)
g = BiGraph()
for query, blines in groupby(blast, key=lambda x: x.query):
blines = list(blines)
iterator = combinations(blines, 2) if clique else pairwise(blines)
for a, b in iterator:
asub, bsub = a.subject, b.subject
if asub == bsub:
continue
arange = (a.query, a.qstart, a.qstop, "+")
brange = (b.query, b.qstart, b.qstop, "+")
dist, oo = range_distance(arange, brange, distmode="ee")
if dist > maxdist:
continue
atag = ">" if a.orientation == "+" else "<"
btag = ">" if b.orientation == "+" else "<"
g.add_edge(BiEdge(asub, bsub, atag, btag))
g.write("graph.txt")
#g.draw("graph.pdf")
logging.debug(str(g))
paths = []
for path in g.iter_paths():
m, oo = g.path(path)
if len(oo) == 1: # Singleton path
continue
paths.append(oo)
if opts.verbose:
print m
print oo
npaths = len(paths)
ntigs = sum(len(x) for x in paths)
logging.debug("Graph decomposed to {0} paths with {1} components.".\
format(npaths, ntigs))
agpfile = blastfile + ".agp"
sizes = Sizes(subjectfasta)
fwagp = open(agpfile, "w")
scaffolded = set()
for i, oo in enumerate(paths):
ctgorder = [(str(ctg), ("+" if strand else "-")) \
for ctg, strand in oo]
scaffolded |= set(ctg for ctg, strand in ctgorder)
object = "pmol_{0:04d}".format(i)
order_to_agp(object, ctgorder, sizes.mapping, fwagp)
# Get the singletons as well
nsingletons = 0
for ctg, size in sizes.iter_sizes():
if ctg in scaffolded:
continue
ctgorder = [(ctg, "+")]
object = ctg
order_to_agp(object, ctgorder, sizes.mapping, fwagp)
nsingletons += 1
logging.debug("Written {0} unscaffolded singletons.".format(nsingletons))
fwagp.close()
logging.debug("AGP file written to `{0}`.".format(agpfile))
def fromblast(args):
"""
%prog fromblast blastfile subject.fasta
Generate path from BLAST file. If multiple subjects map to the same query,
an edge is constructed between them (with the link provided by the query).
The BLAST file MUST be filtered, chained, supermapped.
"""
from jcvi.formats.blast import sort
from jcvi.utils.range import range_distance
p = OptionParser(fromblast.__doc__)
p.add_option(
"--clique",
default=False,
action="store_true",
help="Populate clique instead of linear path [default: %default]")
p.add_option(
"--maxdist",
default=100000,
type="int",
help="Create edge within certain distance [default: %default]")
p.add_option("--verbose",
default=False,
action="store_true",
help="Print verbose reports to stdout [default: %default]")
opts, args = p.parse_args(args)
if len(args) != 2:
sys.exit(not p.print_help())
blastfile, subjectfasta = args
clique = opts.clique
maxdist = opts.maxdist
sort([blastfile, "--query"])
blast = BlastSlow(blastfile, sorted=True)
g = BiGraph()
for query, blines in groupby(blast, key=lambda x: x.query):
blines = list(blines)
iterator = combinations(blines, 2) if clique else pairwise(blines)
for a, b in iterator:
asub, bsub = a.subject, b.subject
if asub == bsub:
continue
arange = (a.query, a.qstart, a.qstop, "+")
brange = (b.query, b.qstart, b.qstop, "+")
dist, oo = range_distance(arange, brange, distmode="ee")
if dist > maxdist:
continue
atag = ">" if a.orientation == "+" else "<"
btag = ">" if b.orientation == "+" else "<"
g.add_edge(BiEdge(asub, bsub, atag, btag))
g.write("graph.txt")
#g.draw("graph.pdf")
logging.debug(str(g))
paths = []
for path in g.iter_paths():
m, oo = g.path(path)
if len(oo) == 1: # Singleton path
continue
paths.append(oo)
if opts.verbose:
print m
print oo
npaths = len(paths)
ntigs = sum(len(x) for x in paths)
logging.debug("Graph decomposed to {0} paths with {1} components.".\
format(npaths, ntigs))
agpfile = blastfile + ".agp"
sizes = Sizes(subjectfasta)
fwagp = open(agpfile, "w")
scaffolded = set()
for i, oo in enumerate(paths):
ctgorder = [(str(ctg), ("+" if strand else "-")) \
for ctg, strand in oo]
scaffolded |= set(ctg for ctg, strand in ctgorder)
object = "pmol_{0:04d}".format(i)
order_to_agp(object, ctgorder, sizes.mapping, fwagp)
# Get the singletons as well
nsingletons = 0
for ctg, size in sizes.iter_sizes():
if ctg in scaffolded:
continue
ctgorder = [(ctg, "+")]
object = ctg
order_to_agp(object, ctgorder, sizes.mapping, fwagp)
nsingletons += 1
logging.debug("Written {0} unscaffolded singletons.".format(nsingletons))
fwagp.close()
logging.debug("AGP file written to `{0}`.".format(agpfile))
def pastegenes(args):
"""
%prog pastegenes coverage.list old.genes.bed new.genes.bed old.assembly
Paste in zero or low coverage genes. For a set of neighboring genes
missing, add the whole cassette as unplaced scaffolds. For singletons the
program will try to make a patch.
"""
from jcvi.formats.base import DictFile
from jcvi.utils.cbook import gene_name
p = OptionParser(pastegenes.__doc__)
p.add_option(
"--cutoff",
default=90,
type="int",
help="Coverage cutoff to call gene missing",
)
p.add_option(
"--flank",
default=2000,
type="int",
help="Get the seq of size on two ends",
)
p.add_option(
"--maxsize",
default=50000,
type="int",
help="Maximum size of patchers to be replaced",
)
opts, args = p.parse_args(args)
if len(args) != 4:
sys.exit(not p.print_help())
coveragefile, oldbed, newbed, oldassembly = args
cutoff = opts.cutoff
flank = opts.flank
maxsize = opts.maxsize
coverage = DictFile(coveragefile, valuepos=2, cast=float)
obed = Bed(oldbed)
order = obed.order
bed = [x for x in obed if x.accn in coverage]
key = lambda x: coverage[x.accn] >= cutoff
extrabed = "extra.bed"
extendbed = "extend.bed"
pastebed = "paste.bed"
fw = open(extrabed, "w")
fwe = open(extendbed, "w")
fwp = open(pastebed, "w")
fw_ids = open(extendbed + ".ids", "w")
singletons, large, large_genes = 0, 0, 0
for chr, chrbed in groupby(bed, key=lambda x: x.seqid):
chrbed = list(chrbed)
for good, beds in groupby(chrbed, key=key):
if good:
continue
beds = list(beds)
blocksize = len(set([gene_name(x.accn) for x in beds]))
if blocksize == 1:
singletons += 1
accn = beds[0].accn
gi, gb = order[accn]
leftb = obed[gi - 1]
rightb = obed[gi + 1]
leftr = leftb.range
rightr = rightb.range
cur = gb.range
distance_to_left, oo = range_distance(leftr, cur)
distance_to_right, oo = range_distance(cur, rightr)
span, oo = range_distance(leftr, rightr)
if distance_to_left <= distance_to_right and distance_to_left > 0:
label = "LEFT"
else:
label = "RIGHT"
if 0 < span <= maxsize:
print(
"\t".join(
str(x) for x in (chr, leftb.start, rightb.end, gb.accn)
),
file=fwp,
)
print(leftb, file=fwe)
print(gb, file=fwe)
print(rightb, file=fwe)
print(
"L:{0} R:{1} [{2}]".format(
distance_to_left, distance_to_right, label
),
file=fwe,
)
print(gb.accn, file=fw_ids)
continue
large += 1
large_genes += blocksize
ranges = [(x.start, x.end) for x in beds]
rmin, rmax = range_minmax(ranges)
rmin -= flank
rmax += flank
name = "-".join((beds[0].accn, beds[-1].accn))
print("\t".join(str(x) for x in (chr, rmin - 1, rmax, name)), file=fw)
fw.close()
fwe.close()
extrabed = mergeBed(extrabed, d=flank, nms=True)
fastaFromBed(extrabed, oldassembly, name=True)
summary([extrabed])
logging.debug("Singleton blocks : {0}".format(singletons))
logging.debug("Large blocks : {0} ({1} genes)".format(large, large_genes))
def report_pairs(data, cutoff=0, mateorientation=None,
pairsfile=None, insertsfile=None, rclip=1, ascii=False, bins=20,
distmode="ss"):
"""
This subroutine is used by the pairs function in blast.py and cas.py.
Reports number of fragments and pairs as well as linked pairs
"""
from jcvi.utils.cbook import percentage
allowed_mateorientations = ("++", "--", "+-", "-+")
if mateorientation:
assert mateorientation in allowed_mateorientations
num_fragments, num_pairs = 0, 0
all_dist = []
linked_dist = []
# +- (forward-backward) is `innie`, -+ (backward-forward) is `outie`
orientations = defaultdict(int)
# clip how many chars from end of the read name to get pair name
key = (lambda x: x.accn[:-rclip]) if rclip else (lambda x: x.accn)
data.sort(key=key)
if pairsfile:
pairsfw = open(pairsfile, "w")
if insertsfile:
insertsfw = open(insertsfile, "w")
for pe, lines in groupby(data, key=key):
lines = list(lines)
if len(lines) != 2:
num_fragments += len(lines)
continue
num_pairs += 1
a, b = lines
asubject, astart, astop = a.seqid, a.start, a.end
bsubject, bstart, bstop = b.seqid, b.start, b.end
aquery, bquery = a.accn, b.accn
astrand, bstrand = a.strand, b.strand
dist, orientation = range_distance(\
(asubject, astart, astop, astrand),
(bsubject, bstart, bstop, bstrand),
distmode=distmode)
if dist >= 0:
all_dist.append((dist, orientation, aquery, bquery))
# select only pairs with certain orientations - e.g. innies, outies, etc.
if mateorientation:
all_dist = [x for x in all_dist if x[1] == mateorientation]
# try to infer cutoff as twice the median until convergence
if cutoff <= 0:
dists = np.array([x[0] for x in all_dist], dtype="int")
p0 = np.median(dists)
cutoff = int(2 * p0) # initial estimate
cutoff = int(math.ceil(cutoff / bins)) * bins
logging.debug("Insert size cutoff set to {0}, ".format(cutoff) +
"use '--cutoff' to override")
for dist, orientation, aquery, bquery in all_dist:
if dist > cutoff:
continue
linked_dist.append(dist)
if pairsfile:
print >> pairsfw, "{0}\t{1}\t{2}".format(aquery, bquery, dist)
orientations[orientation] += 1
print >>sys.stderr, "%d fragments, %d pairs" % (num_fragments, num_pairs)
num_links = len(linked_dist)
linked_dist = np.array(linked_dist, dtype="int")
linked_dist = np.sort(linked_dist)
meandist = np.mean(linked_dist)
stdev = np.std(linked_dist)
p0 = np.median(linked_dist)
p1 = linked_dist[int(num_links * .025)]
p2 = linked_dist[int(num_links * .975)]
meandist, stdev = int(meandist), int(stdev)
p0 = int(p0)
print >>sys.stderr, "%d pairs (%.1f%%) are linked (cutoff=%d)" % \
(num_links, num_links * 100. / num_pairs, cutoff)
print >>sys.stderr, "mean distance between mates: {0} +/- {1}".\
format(meandist, stdev)
print >>sys.stderr, "median distance between mates: {0}".format(p0)
print >>sys.stderr, "95% distance range: {0} - {1}".format(p1, p2)
print >>sys.stderr, "\nOrientations:"
orientation_summary = []
for orientation, count in sorted(orientations.items()):
o = "{0}:{1}".format(orientation, \
percentage(count, num_links, denominator=False))
orientation_summary.append(o.split()[0])
print >>sys.stderr, o
if insertsfile:
from jcvi.graphics.histogram import histogram
print >>insertsfw, "\n".join(str(x) for x in linked_dist)
insertsfw.close()
prefix = insertsfile.rsplit(".", 1)[0]
osummary = " ".join(orientation_summary)
title="{0} ({1}; median dist:{2})".format(prefix, osummary, p0)
histogram(insertsfile, vmin=0, vmax=cutoff, bins=bins,
xlabel="Insertsize", title=title, ascii=ascii)
if op.exists(insertsfile):
os.remove(insertsfile)
return meandist, stdev, p0, p1, p2