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Original implementation of the glomeruli segmentation, published by Klingberg et. al 2017
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applied-systems-biology/python2-custom-segment-glomeruli
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README ------------------------------------------------------------------------------- This package contains code for counting glomeruli in Light-Sheet microscopy images of kidney. Full details of the alogrithm can be found in the paper Klingberg et al. (2017) Fully Automated Evaluation of Total Glomerular Number and Capillary Tuft Size in Nephritic Kidneys Using Lightsheet Microscopy, J. Am. Soc. Nephrol., 28: 452-459. If any part of this code is used for academic purposes please cite the paper above. Contents ------------------------------------------------------------------------------- analyze_kidney.py: Script for counting glomeruli compute_volume.py: Script for computing the volume of the kidney LICENCE: Licencing details of this software (BSD License 2.0). include/boost.py: functions for parallel image processing include/filelib.py: functions for working with files Requirements: ------------------------------------------------------------------------------- Python 2.7+: Code is developed and tested using Python 2.7.3 numpy: Tested on numpy 1.9.1. http://www.numpy.org/ Scipy.ndimage: Tested on scipy.ndimage 2.0 http://www.scipy.org/ mahotas: Tested on Mahotas 1.0.3 argparse: Tested on argparse 1.1 pandas: Tested on pandas 0.15.2 http://pandas.pydata.org/ Usage: ------------------------------------------------------------------------------- To count glomeruli: Run in command line: ``python analyze_kidney.py -i settings.csv`` To compute kindney volume: Run in command line: ``python compute_volume.py -i settings.csv``
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