Anaconda Python3.5+
Packages needed:
- numpy 1.10+
- scipy 0.17+
- pandas 0.18+
- networkx 1.10+
- scikit-learn 0.17+
- pyteomics 3.3+
pip install numpy scipy pandas networkx scikit-learn pyteomicsconda env create -f environment.yml
source activate diffacto_35run_diffacto.py [-h] -i I [-db [DB]] [-samples [SAMPLES]] [-log2 LOG2]
[-normalize {average,median,GMM,None}]
[-farms_mu FARMS_MU] [-farms_alpha FARMS_ALPHA]
[-reference REFERENCE] [-min_samples MIN_SAMPLES]
[-use_unique USE_UNIQUE]
[-impute_threshold IMPUTE_THRESHOLD]
[-cutoff_weight CUTOFF_WEIGHT] [-fast FAST] [-out OUT]
[-mc_out MC_OUT]
optional arguments:
-h, --help show this help message and exit
-i I Peptides abundances in CSV format. The first row
should contain names for all samples. The first column
should contain unique peptide sequences. Missing
values should be empty instead of zeros. (default:
None)
-db [DB] Protein database in FASTA format. If None, the peptide
file must have protein ID(s) in the second column.
(default: None)
-samples [SAMPLES] File of the sample list. One run and its sample group
per line, separated by tab. If None, read from peptide
file headings, then each run will be summarized as a
group. (default: None)
-log2 LOG2 Input abundances are in log scale (True) or linear
scale (False) (default: False)
-normalize {average,median,GMM,None}
Method for sample-wise normalization. (default: None)
-farms_mu FARMS_MU Hyperparameter mu (default: 0.1)
-farms_alpha FARMS_ALPHA
Hyperparameter weight of prior probability (default:
0.1)
-reference REFERENCE Names of reference sample groups (separated by
semicolon) (default: average)
-min_samples MIN_SAMPLES
Minimum number of samples peptides needed to be
quantified in (default: 1)
-use_unique USE_UNIQUE
Use unique peptides only (default: False)
-impute_threshold IMPUTE_THRESHOLD
Minimum fraction of missing values in the group.
Impute missing values if missing fraction is larger
than the threshold. (default: 0.99)
-cutoff_weight CUTOFF_WEIGHT
Peptides weighted lower than the cutoff will be
excluded (default: 0.5)
-fast FAST Allow early termination in EM calculation when noise
is sufficiently small. (default: False)
-out OUT Path to output file (writing in TSV format).
-mc_out MC_OUT Path to MCFDR output (writing in TSV format).
(default: None)Example -----
- Peptide abundances recorded in log scale. map peptides to the protein database HUMAN.fa, using GMM (Gaussian Mixture Model) for per-sample normalization, read sample groups in the file sampleLables.txt, and output protein quantification result to the file protein.txt. Peptide abundance will be scaled by comparing average abundances of all samples.
python run_diffacto.py -i peptides.csv -log2 True -db HUMAN.fa -normalize GMM -samples sampleLables.txt -out protein.txt- Peptide abundances recorded in linear scale, using median abundances for per-sample normalization, read sample groups in the file sampleLables.txt, and output protein quantification result to the file protein.txt. Peptide abundance will be scaled by comparing to average abundances of samples labeled as of Sample1 and Sample3 in the sample list. Use peptides unique to the protein and quantified at least in 20 samples. For a given group of sample, if missing values consist more than 70% of the results, impute missing values at half of the minimum non-missing abundance. Apply sequential Monte Carlo permutation tests and estimate MCFDR for differentially expressed proteins.
python run_diffacto.py -i peptides.csv -out protein.txt -normalize median -samples sampleLables.txt -ref Sample1;Sample3 -use_unique True -min_samples 20 -impute_threshold 0.7 -mc_out protein.MCFDR.txt