def main(args):
if ' ' in qconfig.QUAST_HOME:
logger.error(
'QUAST does not support spaces in paths. \n'
'You are trying to run it from ' + str(qconfig.QUAST_HOME) + '\n'
'Please, put QUAST in a different directory, then try again.\n',
to_stderr=True,
exit_with_code=3)
if not args:
qconfig.usage(meta=True)
sys.exit(0)
genes = []
operons = []
html_report = qconfig.html_report
make_latest_symlink = True
ref_txt_fpath = None
try:
options, contigs_fpaths = getopt.gnu_getopt(args,
qconfig.short_options,
qconfig.long_options)
except getopt.GetoptError:
_, exc_value, _ = sys.exc_info()
print >> sys.stderr, exc_value
print >> sys.stderr
qconfig.usage(meta=True)
sys.exit(2)
quast_py_args = args[:]
test_mode = False
for opt, arg in options:
if opt in ('-d', '--debug'):
options.remove((opt, arg))
qconfig.debug = True
logger.set_up_console_handler(debug=True)
elif opt == '--test' or opt == '--test-no-ref':
options.remove((opt, arg))
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt)
options += [('-o', 'quast_test_output')]
if opt == '--test':
options += [('-R', ','.join([
os.path.join(qconfig.QUAST_HOME, 'test_data',
'meta_ref_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data',
'meta_ref_2.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data',
'meta_ref_3.fasta')
]))]
contigs_fpaths += [
os.path.join(qconfig.QUAST_HOME, 'test_data',
'meta_contigs_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data',
'meta_contigs_2.fasta')
]
test_mode = True
elif opt.startswith('--help') or opt == '-h':
qconfig.usage(opt == "--help-hidden", meta=True, short=False)
sys.exit(0)
elif opt.startswith('--version') or opt == '-v':
qconfig.print_version(meta=True)
sys.exit(0)
if not contigs_fpaths:
logger.error("You should specify at least one file with contigs!\n")
qconfig.usage(meta=True)
sys.exit(2)
ref_fpaths = []
combined_ref_fpath = ''
reads_fpath_f = ''
reads_fpath_r = ''
output_dirpath = None
labels = None
all_labels_from_dirs = False
for opt, arg in options:
if opt in ('-o', "--output-dir"):
# Removing output dir arg in order to further
# construct other quast calls from this options
if opt in quast_py_args and arg in quast_py_args:
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
output_dirpath = os.path.abspath(arg)
make_latest_symlink = False
elif opt in ('-G', "--genes"):
assert_file_exists(arg, 'genes')
genes += arg
elif opt in ('-O', "--operons"):
assert_file_exists(arg, 'operons')
operons += arg
elif opt in ('-R', "--reference"):
# Removing reference args in order to further
# construct quast calls from this args with other reference options
if opt in quast_py_args and arg in quast_py_args:
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
if os.path.isdir(arg):
ref_fpaths = [
os.path.join(path, file)
for (path, dirs, files) in os.walk(arg) for file in files
if qutils.check_is_fasta_file(file)
]
ref_fpaths.sort()
else:
ref_fpaths = arg.split(',')
for i, ref_fpath in enumerate(ref_fpaths):
assert_file_exists(ref_fpath, 'reference')
ref_fpaths[i] = ref_fpath
elif opt == '--max-ref-number':
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
qconfig.max_references = int(arg)
if qconfig.max_references < 0:
qconfig.max_references = 0
elif opt in ('-m', "--min-contig"):
qconfig.min_contig = int(arg)
elif opt in ('-t', "--threads"):
qconfig.max_threads = int(arg)
if qconfig.max_threads < 1:
qconfig.max_threads = 1
elif opt in ('-l', '--labels'):
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
labels = quast.parse_labels(arg, contigs_fpaths)
elif opt == '-L':
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt)
all_labels_from_dirs = True
elif opt in ('-j', '--save-json'):
pass
elif opt in ('-J', '--save-json-to'):
pass
elif opt == "--contig-thresholds":
pass
elif opt in ('-c', "--mincluster"):
pass
elif opt == "--est-ref-size":
pass
elif opt == "--gene-thresholds":
pass
elif opt in ('-s', "--scaffolds"):
pass
elif opt == "--gage":
pass
elif opt == "--debug":
pass
elif opt in ('-e', "--eukaryote"):
pass
elif opt in ('-f', "--gene-finding"):
pass
elif opt in ('-i', "--min-alignment"):
pass
elif opt in ('-c', "--min-cluster"):
pass
elif opt in ('-a', "--ambiguity-usage"):
pass
elif opt in ('-u', "--use-all-alignments"):
pass
elif opt == "--strict-NA":
pass
elif opt in ('-x', "--extensive-mis-size"):
pass
elif opt == "--meta":
pass
elif opt == '--references-list':
ref_txt_fpath = arg
elif opt == '--glimmer':
pass
elif opt == '--no-snps':
pass
elif opt == '--no-check':
pass
elif opt == '--no-gc':
pass
elif opt == '--no-plots':
pass
elif opt == '--no-html':
html_report = False
elif opt == '--fast': # --no-check, --no-gc, --no-snps will automatically set in QUAST runs
html_report = False
elif opt == '--plots-format':
pass
elif opt == '--memory-efficient':
pass
elif opt == '--silent':
qconfig.silent = True
elif opt in ('-1', '--reads1'):
reads_fpath_f = arg
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
elif opt in ('-2', '--reads2'):
reads_fpath_r = arg
quast_py_args = __remove_from_quast_py_args(
quast_py_args, opt, arg)
elif opt == '--contig-alignment-html':
qconfig.create_contig_alignment_html = True
else:
logger.error('Unknown option: %s. Use -h for help.' %
(opt + ' ' + arg),
to_stderr=True,
exit_with_code=2)
for c_fpath in contigs_fpaths:
assert_file_exists(c_fpath, 'contigs')
labels = quast.process_labels(contigs_fpaths, labels, all_labels_from_dirs)
for contigs_fpath in contigs_fpaths:
if contigs_fpath in quast_py_args:
quast_py_args.remove(contigs_fpath)
# Directories
output_dirpath, _, _ = quast._set_up_output_dir(output_dirpath,
None,
make_latest_symlink,
save_json=False)
corrected_dirpath = os.path.join(output_dirpath, qconfig.corrected_dirname)
logger.set_up_file_handler(output_dirpath)
args = [os.path.realpath(__file__)]
for k, v in options:
args.extend([k, v])
args.extend(contigs_fpaths)
logger.print_command_line(args, wrap_after=None)
logger.start()
qconfig.set_max_threads(logger)
########################################################################
from libs import reporting
reload(reporting)
if os.path.isdir(corrected_dirpath):
shutil.rmtree(corrected_dirpath)
os.mkdir(corrected_dirpath)
# PROCESSING REFERENCES
if ref_fpaths:
logger.main_info()
logger.main_info('Reference(s):')
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(ref_fpaths, corrected_dirpath)
# PROCESSING CONTIGS
logger.main_info()
logger.main_info('Contigs:')
assemblies, correct_assemblies = _correct_contigs(contigs_fpaths,
output_dirpath, labels)
if not assemblies:
logger.error(
"None of the assembly files contains correct contigs. "
"Please, provide different files or decrease --min-contig threshold."
)
return 4
# Running QUAST(s)
quast_py_args += ['--meta']
downloaded_refs = False
# SEARCHING REFERENCES
if not ref_fpaths:
logger.main_info()
if qconfig.max_references == 0:
logger.notice(
"Maximum number of references (--max-ref-number) is set to 0, search in SILVA 16S rRNA database is disabled"
)
else:
if ref_txt_fpath:
logger.main_info(
"List of references was provided, starting to download reference genomes from NCBI..."
)
else:
logger.main_info(
"No references are provided, starting to search for reference genomes in SILVA 16S rRNA database "
"and to download them from NCBI...")
downloaded_dirpath = os.path.join(output_dirpath,
qconfig.downloaded_dirname)
if not os.path.isdir(downloaded_dirpath):
os.mkdir(downloaded_dirpath)
ref_fpaths = search_references_meta.do(assemblies, labels,
downloaded_dirpath,
ref_txt_fpath)
if ref_fpaths:
search_references_meta.is_quast_first_run = True
if not ref_txt_fpath:
downloaded_refs = True
logger.main_info()
logger.main_info('Downloaded reference(s):')
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(ref_fpaths, corrected_dirpath)
elif test_mode and ref_fpaths is None:
logger.error(
'Failed to download or setup SILVA 16S rRNA database for working without '
'references on metagenome datasets!',
to_stderr=True,
exit_with_code=4)
if not ref_fpaths:
# No references, running regular quast with MetaGenemark gene finder
logger.main_info()
logger.notice(
'No references are provided, starting regular QUAST with MetaGeneMark gene finder'
)
_start_quast_main(None,
quast_py_args,
assemblies=assemblies,
output_dirpath=output_dirpath,
exit_on_exception=True)
exit(0)
# Running combined reference
combined_output_dirpath = os.path.join(output_dirpath,
qconfig.combined_output_name)
reads_fpaths = []
if reads_fpath_f:
reads_fpaths.append(reads_fpath_f)
if reads_fpath_r:
reads_fpaths.append(reads_fpath_r)
if reads_fpaths:
bed_fpath = reads_analyzer.do(combined_ref_fpath,
contigs_fpaths,
reads_fpaths,
corrected_ref_fpaths,
os.path.join(combined_output_dirpath,
qconfig.variation_dirname),
external_logger=logger)
if bed_fpath:
quast_py_args += ['--bed-file']
quast_py_args += [bed_fpath]
quast_py_args += ['--combined-ref']
run_name = 'for the combined reference'
logger.main_info()
logger.main_info('Starting quast.py ' + run_name + '...')
total_num_notices = 0
total_num_warnings = 0
total_num_nf_errors = 0
total_num_notifications = (total_num_notices, total_num_warnings,
total_num_nf_errors)
if qconfig.html_report:
from libs.html_saver import json_saver
json_texts = []
else:
json_texts = None
return_code, total_num_notifications, assemblies, labels = _start_quast_main(
run_name,
quast_py_args + ["--ambiguity-usage"] + ['all'],
assemblies=assemblies,
reference_fpath=combined_ref_fpath,
output_dirpath=combined_output_dirpath,
num_notifications_tuple=total_num_notifications,
is_first_run=True)
for arg in args:
if arg in ('-s', "--scaffolds"):
quast_py_args.remove(arg)
if json_texts is not None:
json_texts.append(json_saver.json_text)
search_references_meta.is_quast_first_run = False
genome_info_dirpath = os.path.join(output_dirpath,
qconfig.combined_output_name,
'genome_stats')
genome_info_fpath = os.path.join(genome_info_dirpath, 'genome_info.txt')
if not os.path.exists(genome_info_fpath):
logger.main_info('')
logger.main_info(
'Failed aligning the contigs for all the references. ' +
('Try to restart MetaQUAST with another references.'
if not downloaded_refs else
'Try to use option --max-ref-number to change maximum number of references '
'(per each assembly) to download.'))
logger.main_info('')
quast._cleanup(corrected_dirpath)
logger.main_info('MetaQUAST finished.')
logger.finish_up(numbers=tuple(total_num_notifications),
check_test=test_mode)
return
if downloaded_refs:
logger.main_info()
logger.main_info(
'Excluding downloaded references with low genome fraction from further analysis..'
)
corr_ref_fpaths = remove_unaligned_downloaded_refs(
genome_info_fpath, ref_fpaths, chromosomes_by_refs)
if corr_ref_fpaths and corr_ref_fpaths != ref_fpaths:
logger.main_info()
logger.main_info('Filtered reference(s):')
os.remove(combined_ref_fpath)
contigs_analyzer.ref_labels_by_chromosomes = {}
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(corr_ref_fpaths, corrected_dirpath)
run_name = 'for the corrected combined reference'
logger.main_info()
logger.main_info('Starting quast.py ' + run_name + '...')
return_code, total_num_notifications, assemblies, labels = _start_quast_main(
run_name,
quast_py_args + ["--ambiguity-usage"] + ['all'],
assemblies=assemblies,
reference_fpath=combined_ref_fpath,
output_dirpath=combined_output_dirpath,
num_notifications_tuple=total_num_notifications,
is_first_run=True)
if json_texts is not None:
json_texts = json_texts[:-1]
json_texts.append(json_saver.json_text)
elif corr_ref_fpaths == ref_fpaths:
logger.main_info(
'All downloaded references have genome fraction more than 10%. Nothing was excluded.'
)
else:
logger.main_info(
'All downloaded references have low genome fraction. Nothing was excluded for now.'
)
quast_py_args += ['--no-check-meta']
qconfig.contig_thresholds = ','.join([
str(threshold) for threshold in qconfig.contig_thresholds
if threshold > qconfig.min_contig
])
if not qconfig.contig_thresholds:
qconfig.contig_thresholds = 'None'
quast_py_args = __remove_from_quast_py_args(quast_py_args,
'--contig-thresholds',
qconfig.contig_thresholds)
quast_py_args += ['--contig-thresholds']
quast_py_args += [qconfig.contig_thresholds]
quast_py_args.remove('--combined-ref')
logger.main_info()
logger.main_info(
'Partitioning contigs into bins aligned to each reference..')
assemblies_by_reference, not_aligned_assemblies = _partition_contigs(
assemblies, corrected_ref_fpaths, corrected_dirpath,
os.path.join(combined_output_dirpath, 'contigs_reports',
'alignments_%s.tsv'), labels)
ref_names = []
output_dirpath_per_ref = os.path.join(output_dirpath,
qconfig.per_ref_dirname)
for ref_fpath, ref_assemblies in assemblies_by_reference:
ref_name = qutils.name_from_fpath(ref_fpath)
logger.main_info('')
if not ref_assemblies:
logger.main_info('No contigs were aligned to the reference ' +
ref_name + ', skipping..')
else:
ref_names.append(ref_name)
run_name = 'for the contigs aligned to ' + ref_name
logger.main_info('Starting quast.py ' + run_name)
return_code, total_num_notifications = _start_quast_main(
run_name,
quast_py_args,
assemblies=ref_assemblies,
reference_fpath=ref_fpath,
output_dirpath=os.path.join(output_dirpath_per_ref, ref_name),
exit_on_exception=False,
num_notifications_tuple=total_num_notifications)
if json_texts is not None:
json_texts.append(json_saver.json_text)
# Finally running for the contigs that has not been aligned to any reference
no_unaligned_contigs = True
for assembly in not_aligned_assemblies:
if os.path.isfile(
assembly.fpath) and os.stat(assembly.fpath).st_size != 0:
no_unaligned_contigs = False
break
run_name = 'for the contigs not aligned anywhere'
logger.main_info()
if no_unaligned_contigs:
logger.main_info('Skipping quast.py ' + run_name +
' (everything is aligned!)')
else:
logger.main_info('Starting quast.py ' + run_name + '...')
return_code, total_num_notifications = _start_quast_main(
run_name,
quast_py_args,
assemblies=not_aligned_assemblies,
output_dirpath=os.path.join(output_dirpath,
qconfig.not_aligned_name),
exit_on_exception=False,
num_notifications_tuple=total_num_notifications)
if return_code not in [0, 4]:
logger.error(
'Error running quast.py for the contigs not aligned anywhere')
elif return_code == 4: # no unaligned contigs, i.e. everything aligned
no_unaligned_contigs = True
if not no_unaligned_contigs:
if json_texts is not None:
json_texts.append(json_saver.json_text)
if ref_names:
logger.print_timestamp()
logger.main_info("Summarizing results...")
summary_output_dirpath = os.path.join(output_dirpath,
qconfig.meta_summary_dir)
if not os.path.isdir(summary_output_dirpath):
os.makedirs(summary_output_dirpath)
if html_report and json_texts:
from libs.html_saver import html_saver
html_summary_report_fpath = html_saver.init_meta_report(
output_dirpath)
else:
html_summary_report_fpath = None
from libs import create_meta_summary
metrics_for_plots = reporting.Fields.main_metrics
misassembl_metrics = [
reporting.Fields.MIS_RELOCATION,
reporting.Fields.MIS_TRANSLOCATION, reporting.Fields.MIS_INVERTION,
reporting.Fields.MIS_ISTRANSLOCATIONS
]
create_meta_summary.do(
html_summary_report_fpath, summary_output_dirpath,
combined_output_dirpath, output_dirpath_per_ref, metrics_for_plots,
misassembl_metrics,
ref_names if no_unaligned_contigs else ref_names +
[qconfig.not_aligned_name])
if html_report and json_texts:
from libs import plotter
html_saver.save_colors(output_dirpath,
contigs_fpaths,
plotter.dict_color_and_ls,
meta=True)
html_saver.create_meta_report(output_dirpath, json_texts)
quast._cleanup(corrected_dirpath)
logger.main_info('')
logger.main_info('MetaQUAST finished.')
logger.finish_up(numbers=tuple(total_num_notifications),
check_test=test_mode)
def main(args):
if ' ' in qconfig.QUAST_HOME:
logger.error('QUAST does not support spaces in paths. \n'
'You are trying to run it from ' + str(qconfig.QUAST_HOME) + '\n'
'Please, put QUAST in a different directory, then try again.\n',
to_stderr=True,
exit_with_code=3)
if not args:
qconfig.usage()
sys.exit(0)
reload(qconfig)
try:
options, contigs_fpaths = getopt.gnu_getopt(args, qconfig.short_options, qconfig.long_options)
except getopt.GetoptError:
_, exc_value, _ = sys.exc_info()
print >> sys.stderr, exc_value
print >> sys.stderr
qconfig.usage()
sys.exit(2)
for opt, arg in options[:]:
if opt == '--test' or opt == '--test-sv':
options.remove((opt, arg))
options += [('-o', 'quast_test_output'),
('-R', os.path.join(qconfig.QUAST_HOME, 'test_data', 'reference.fasta.gz')), # for compiling MUMmer
('-O', os.path.join(qconfig.QUAST_HOME, 'test_data', 'operons.gff')),
('-G', os.path.join(qconfig.QUAST_HOME, 'test_data', 'genes.gff')),
('--gage', ''), # for compiling GAGE Java classes
('--gene-finding', ''), ('--eukaryote', ''), ('--glimmer', '')] # for compiling GlimmerHMM
if opt == '--test-sv':
options += [('-1', os.path.join(qconfig.QUAST_HOME, 'test_data', 'reads1.fastq.gz')),
('-2', os.path.join(qconfig.QUAST_HOME, 'test_data', 'reads2.fastq.gz'))]
contigs_fpaths += [os.path.join(qconfig.QUAST_HOME, 'test_data', 'contigs_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data', 'contigs_2.fasta')]
qconfig.test = True
if opt.startswith('--help') or opt == '-h':
qconfig.usage(opt == "--help-hidden", short=False)
sys.exit(0)
elif opt.startswith('--version') or opt == '-v':
qconfig.print_version()
sys.exit(0)
if not contigs_fpaths:
logger.error("You should specify at least one file with contigs!\n")
qconfig.usage()
sys.exit(2)
json_output_dirpath = None
output_dirpath = None
labels = None
all_labels_from_dirs = False
qconfig.is_combined_ref = False
ref_fpath = ''
genes_fpaths = []
operons_fpaths = []
bed_fpath = None
reads_fpath_f = ''
reads_fpath_r = ''
# Yes, this is a code duplicating. But OptionParser is deprecated since version 2.7.
for opt, arg in options:
if opt in ('-d', '--debug'):
qconfig.debug = True
logger.set_up_console_handler(debug=True)
elif opt in ('-o', "--output-dir"):
output_dirpath = os.path.abspath(arg)
qconfig.make_latest_symlink = False
if ' ' in output_dirpath:
logger.error('QUAST does not support spaces in paths. \n'
'You have specified ' + str(output_dirpath) + ' as an output path.\n'
'Please, use a different directory.\n',
to_stderr=True,
exit_with_code=3)
elif opt in ('-G', "--genes"):
genes_fpaths.append(assert_file_exists(arg, 'genes'))
elif opt in ('-O', "--operons"):
operons_fpaths.append(assert_file_exists(arg, 'operons'))
elif opt in ('-R', "--reference"):
ref_fpath = assert_file_exists(arg, 'reference')
elif opt == "--contig-thresholds":
qconfig.contig_thresholds = arg
elif opt in ('-m', "--min-contig"):
qconfig.min_contig = int(arg)
elif opt in ('-t', "--threads"):
qconfig.max_threads = int(arg)
if qconfig.max_threads < 1:
qconfig.max_threads = 1
elif opt in ('-c', "--min-cluster"):
qconfig.min_cluster = int(arg)
elif opt in ('-i', "--min-alignment"):
qconfig.min_alignment = int(arg)
elif opt == "--est-ref-size":
qconfig.estimated_reference_size = int(arg)
elif opt == "--gene-thresholds":
qconfig.genes_lengths = arg
elif opt in ('-j', '--save-json'):
qconfig.save_json = True
elif opt in ('-J', '--save-json-to'):
qconfig.save_json = True
qconfig.make_latest_symlink = False
json_output_dirpath = arg
elif opt == '--err-fpath': # for web-quast
qconfig.save_error = True
qconfig.error_log_fname = arg
elif opt in ('-s', "--scaffolds"):
qconfig.scaffolds = True
elif opt == "--gage":
qconfig.with_gage = True
elif opt in ('-e', "--eukaryote"):
qconfig.prokaryote = False
elif opt in ('-f', "--gene-finding"):
qconfig.gene_finding = True
elif opt in ('-a', "--ambiguity-usage"):
if arg in ["none", "one", "all"]:
qconfig.ambiguity_usage = arg
elif opt in ('-u', "--use-all-alignments"):
qconfig.use_all_alignments = True
elif opt == "--strict-NA":
qconfig.strict_NA = True
elif opt in ('-x', "--extensive-mis-size"):
if int(arg) <= qconfig.MAX_INDEL_LENGTH:
logger.error("--extensive-mis-size should be greater than maximum indel length (%d)!"
% qconfig.MAX_INDEL_LENGTH, 1, to_stderr=True)
qconfig.extensive_misassembly_threshold = int(arg)
elif opt == '--no-snps':
qconfig.show_snps = False
elif opt == '--no-plots':
qconfig.draw_plots = False
elif opt == '--no-html':
qconfig.html_report = False
elif opt == '--no-check':
qconfig.no_check = True
elif opt == '--no-gc':
qconfig.no_gc = True
elif opt == '--fast': # --no-gc, --no-plots, --no-snps
#qconfig.no_check = True # too risky to include
qconfig.no_gc = True
qconfig.show_snps = False
qconfig.draw_plots = False
qconfig.html_report = False
elif opt == '--plots-format':
if arg.lower() in qconfig.supported_plot_extensions:
qconfig.plot_extension = arg.lower()
else:
logger.error('Format "%s" is not supported. Please, use one of the supported formats: %s.' %
(arg, ', '.join(qconfig.supported_plot_extensions)), to_stderr=True, exit_with_code=2)
elif opt == '--meta':
qconfig.meta = True
elif opt == '--no-check-meta':
qconfig.no_check = True
qconfig.no_check_meta = True
elif opt == '--references-list':
pass
elif opt in ('-l', '--labels'):
labels = parse_labels(arg, contigs_fpaths)
elif opt == '-L':
all_labels_from_dirs = True
elif opt == '--glimmer':
qconfig.glimmer = True
elif opt == '--combined-ref':
qconfig.is_combined_ref = True
elif opt == '--memory-efficient':
qconfig.memory_efficient = True
elif opt == '--silent':
qconfig.silent = True
elif opt in ('-1', '--reads1'):
reads_fpath_f = arg
elif opt in ('-2', '--reads2'):
reads_fpath_r = arg
elif opt == '--bed-file':
bed_fpath = arg
elif opt == '--contig-alignment-html':
qconfig.create_contig_alignment_html = True
else:
logger.error('Unknown option: %s. Use -h for help.' % (opt + ' ' + arg), to_stderr=True, exit_with_code=2)
for contigs_fpath in contigs_fpaths:
assert_file_exists(contigs_fpath, 'contigs')
labels = process_labels(contigs_fpaths, labels, all_labels_from_dirs)
output_dirpath, json_output_dirpath, existing_alignments = \
_set_up_output_dir(output_dirpath, json_output_dirpath, qconfig.make_latest_symlink, qconfig.save_json)
corrected_dirpath = os.path.join(output_dirpath, qconfig.corrected_dirname)
logger.set_up_file_handler(output_dirpath)
args = [os.path.realpath(__file__)]
for k, v in options: args.extend([k, v])
args.extend(contigs_fpaths)
logger.print_command_line(args, wrap_after=None, is_main=True)
logger.start()
if existing_alignments:
logger.main_info()
logger.notice("Output directory already exists. Existing Nucmer alignments can be used.")
qutils.remove_reports(output_dirpath)
if qconfig.contig_thresholds == "None":
qconfig.contig_thresholds = []
else:
qconfig.contig_thresholds = map(int, qconfig.contig_thresholds.split(","))
if qconfig.genes_lengths == "None":
qconfig.genes_lengths = []
else:
qconfig.genes_lengths = map(int, qconfig.genes_lengths.split(","))
qconfig.set_max_threads(logger)
logger.main_info()
logger.print_params()
########################################################################
from libs import reporting
reload(reporting)
if qconfig.is_combined_ref:
corrected_dirpath = os.path.join(output_dirpath, '..', qconfig.corrected_dirname)
else:
if os.path.isdir(corrected_dirpath):
shutil.rmtree(corrected_dirpath)
os.mkdir(corrected_dirpath)
# PROCESSING REFERENCE
if ref_fpath:
logger.main_info()
logger.main_info('Reference:')
ref_fpath = _correct_reference(ref_fpath, corrected_dirpath)
else:
ref_fpath = ''
# PROCESSING CONTIGS
logger.main_info()
logger.main_info('Contigs:')
contigs_fpaths, old_contigs_fpaths = _correct_contigs(contigs_fpaths, corrected_dirpath, reporting, labels)
for contigs_fpath in contigs_fpaths:
report = reporting.get(contigs_fpath)
report.add_field(reporting.Fields.NAME, qutils.label_from_fpath(contigs_fpath))
qconfig.assemblies_num = len(contigs_fpaths)
reads_fpaths = []
if reads_fpath_f:
reads_fpaths.append(reads_fpath_f)
if reads_fpath_r:
reads_fpaths.append(reads_fpath_r)
if reads_fpaths:
bed_fpath = reads_analyzer.do(ref_fpath, contigs_fpaths, reads_fpaths, None,
os.path.join(output_dirpath, qconfig.variation_dirname),
external_logger=logger)
if not contigs_fpaths:
logger.error("None of the assembly files contains correct contigs. "
"Please, provide different files or decrease --min-contig threshold.",
fake_if_nested_run=True)
return 4
qconfig.assemblies_fpaths = contigs_fpaths
if qconfig.with_gage:
########################################################################
### GAGE
########################################################################
if not ref_fpath:
logger.warning("GAGE can't be run without a reference and will be skipped.")
else:
from libs import gage
gage.do(ref_fpath, contigs_fpaths, output_dirpath)
# Where all pdfs will be saved
all_pdf_fpath = os.path.join(output_dirpath, qconfig.plots_fname)
all_pdf_file = None
if qconfig.draw_plots or qconfig.html_report:
from libs import plotter # Do not remove this line! It would lead to a warning in matplotlib.
try:
from matplotlib.backends.backend_pdf import PdfPages
all_pdf_file = PdfPages(all_pdf_fpath)
except:
all_pdf_file = None
if json_output_dirpath:
from libs.html_saver import json_saver
if json_saver.simplejson_error:
json_output_dirpath = None
########################################################################
### Stats and plots
########################################################################
from libs import basic_stats
basic_stats.do(ref_fpath, contigs_fpaths, os.path.join(output_dirpath, 'basic_stats'),
json_output_dirpath, output_dirpath)
aligned_contigs_fpaths = []
aligned_lengths_lists = []
contig_alignment_plot_fpath = None
if ref_fpath:
########################################################################
### former PLANTAKOLYA, PLANTAGORA
########################################################################
from libs import contigs_analyzer
nucmer_statuses, aligned_lengths_per_fpath = contigs_analyzer.do(
ref_fpath, contigs_fpaths, qconfig.prokaryote, os.path.join(output_dirpath, 'contigs_reports'), old_contigs_fpaths, bed_fpath)
for contigs_fpath in contigs_fpaths:
if nucmer_statuses[contigs_fpath] == contigs_analyzer.NucmerStatus.OK:
aligned_contigs_fpaths.append(contigs_fpath)
aligned_lengths_lists.append(aligned_lengths_per_fpath[contigs_fpath])
# Before continue evaluating, check if nucmer didn't skip all of the contigs files.
detailed_contigs_reports_dirpath = None
if len(aligned_contigs_fpaths) and ref_fpath:
detailed_contigs_reports_dirpath = os.path.join(output_dirpath, 'contigs_reports')
########################################################################
### NAx and NGAx ("aligned Nx and NGx")
########################################################################
from libs import aligned_stats
aligned_stats.do(
ref_fpath, aligned_contigs_fpaths, output_dirpath, json_output_dirpath,
aligned_lengths_lists, os.path.join(output_dirpath, 'aligned_stats'))
########################################################################
### GENOME_ANALYZER
########################################################################
from libs import genome_analyzer
genome_analyzer.do(
ref_fpath, aligned_contigs_fpaths, output_dirpath, json_output_dirpath,
genes_fpaths, operons_fpaths, detailed_contigs_reports_dirpath, os.path.join(output_dirpath, 'genome_stats'))
if qconfig.gene_finding or qconfig.glimmer:
if qconfig.glimmer:
########################################################################
### Glimmer
########################################################################
from libs import glimmer
glimmer.do(contigs_fpaths, qconfig.genes_lengths, os.path.join(output_dirpath, 'predicted_genes'))
else:
########################################################################
### GeneMark
########################################################################
from libs import genemark
genemark.do(contigs_fpaths, qconfig.genes_lengths, os.path.join(output_dirpath, 'predicted_genes'), qconfig.prokaryote,
qconfig.meta)
else:
logger.main_info("")
logger.notice("Genes are not predicted by default. Use --gene-finding option to enable it.")
########################################################################
reports_fpaths, transposed_reports_fpaths = reporting.save_total(output_dirpath)
########################################################################
### LARGE DRAWING TASKS
########################################################################
if qconfig.draw_plots:
logger.print_timestamp()
logger.main_info('Drawing large plots...')
logger.main_info('This may take a while: press Ctrl-C to skip this step..')
try:
if detailed_contigs_reports_dirpath and qconfig.show_snps:
contig_report_fpath_pattern = os.path.join(detailed_contigs_reports_dirpath, 'contigs_report_%s.stdout')
else:
contig_report_fpath_pattern = None
number_of_steps = sum([int(bool(value)) for value in [contig_report_fpath_pattern, all_pdf_file]])
if contig_report_fpath_pattern:
########################################################################
### VISUALIZE CONTIG ALIGNMENT
########################################################################
logger.main_info(' 1 of %d: Creating contig alignment plot...' % number_of_steps)
from libs import contig_alignment_plotter
contig_alignment_plot_fpath = contig_alignment_plotter.do(
contigs_fpaths, contig_report_fpath_pattern,
output_dirpath, ref_fpath, similar=True)
if all_pdf_file:
# full report in PDF format: all tables and plots
logger.main_info(' %d of %d: Creating PDF with all tables and plots...' % (number_of_steps, number_of_steps))
plotter.fill_all_pdf_file(all_pdf_file)
logger.main_info('Done')
except KeyboardInterrupt:
logger.main_info('..step skipped!')
os.remove(all_pdf_fpath)
########################################################################
### TOTAL REPORT
########################################################################
logger.print_timestamp()
logger.main_info('RESULTS:')
logger.main_info(' Text versions of total report are saved to ' + reports_fpaths)
logger.main_info(' Text versions of transposed total report are saved to ' + transposed_reports_fpaths)
if json_output_dirpath:
json_saver.save_total_report(json_output_dirpath, qconfig.min_contig, ref_fpath)
if qconfig.html_report:
from libs.html_saver import html_saver
html_saver.save_colors(output_dirpath, contigs_fpaths, plotter.dict_color_and_ls)
html_saver.save_total_report(output_dirpath, qconfig.min_contig, ref_fpath)
if os.path.isfile(all_pdf_fpath):
logger.main_info(' PDF version (tables and plots) saved to ' + all_pdf_fpath)
if contig_alignment_plot_fpath:
logger.main_info(' Contig alignment plot: %s' % contig_alignment_plot_fpath)
_cleanup(corrected_dirpath)
logger.finish_up(check_test=qconfig.test)
return 0
def main(args):
if ' ' in qconfig.QUAST_HOME:
logger.error('QUAST does not support spaces in paths. \n'
'You are trying to run it from ' + str(qconfig.QUAST_HOME) + '\n'
'Please, put QUAST in a different directory, then try again.\n',
to_stderr=True,
exit_with_code=3)
if not args:
qconfig.usage(meta=True)
sys.exit(0)
genes = []
operons = []
html_report = qconfig.html_report
make_latest_symlink = True
ref_txt_fpath = None
try:
options, contigs_fpaths = getopt.gnu_getopt(args, qconfig.short_options, qconfig.long_options)
except getopt.GetoptError:
_, exc_value, _ = sys.exc_info()
print >> sys.stderr, exc_value
print >> sys.stderr
qconfig.usage(meta=True)
sys.exit(2)
quast_py_args = args[:]
test_mode = False
for opt, arg in options:
if opt in ('-d', '--debug'):
options.remove((opt, arg))
qconfig.debug = True
logger.set_up_console_handler(debug=True)
elif opt == '--test' or opt == '--test-no-ref':
options.remove((opt, arg))
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt)
options += [('-o', 'quast_test_output')]
if opt == '--test':
options += [('-R', ','.join([os.path.join(qconfig.QUAST_HOME, 'test_data', 'meta_ref_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data', 'meta_ref_2.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data', 'meta_ref_3.fasta')]))]
contigs_fpaths += [os.path.join(qconfig.QUAST_HOME, 'test_data', 'meta_contigs_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data', 'meta_contigs_2.fasta')]
test_mode = True
elif opt.startswith('--help') or opt == '-h':
qconfig.usage(opt == "--help-hidden", meta=True, short=False)
sys.exit(0)
elif opt.startswith('--version') or opt == '-v':
qconfig.print_version(meta=True)
sys.exit(0)
if not contigs_fpaths:
logger.error("You should specify at least one file with contigs!\n")
qconfig.usage(meta=True)
sys.exit(2)
ref_fpaths = []
combined_ref_fpath = ''
reads_fpath_f = ''
reads_fpath_r = ''
output_dirpath = None
labels = None
all_labels_from_dirs = False
for opt, arg in options:
if opt in ('-o', "--output-dir"):
# Removing output dir arg in order to further
# construct other quast calls from this options
if opt in quast_py_args and arg in quast_py_args:
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
output_dirpath = os.path.abspath(arg)
make_latest_symlink = False
elif opt in ('-G', "--genes"):
assert_file_exists(arg, 'genes')
genes += arg
elif opt in ('-O', "--operons"):
assert_file_exists(arg, 'operons')
operons += arg
elif opt in ('-R', "--reference"):
# Removing reference args in order to further
# construct quast calls from this args with other reference options
if opt in quast_py_args and arg in quast_py_args:
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
if os.path.isdir(arg):
ref_fpaths = [os.path.join(path,file) for (path, dirs, files) in os.walk(arg) for file in files if qutils.check_is_fasta_file(file)]
ref_fpaths.sort()
else:
ref_fpaths = arg.split(',')
for i, ref_fpath in enumerate(ref_fpaths):
assert_file_exists(ref_fpath, 'reference')
ref_fpaths[i] = ref_fpath
elif opt == '--max-ref-number':
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
qconfig.max_references = int(arg)
if qconfig.max_references < 0:
qconfig.max_references = 0
elif opt in ('-m', "--min-contig"):
qconfig.min_contig = int(arg)
elif opt in ('-t', "--threads"):
qconfig.max_threads = int(arg)
if qconfig.max_threads < 1:
qconfig.max_threads = 1
elif opt in ('-l', '--labels'):
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
labels = quast.parse_labels(arg, contigs_fpaths)
elif opt == '-L':
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt)
all_labels_from_dirs = True
elif opt in ('-j', '--save-json'):
pass
elif opt in ('-J', '--save-json-to'):
pass
elif opt == "--contig-thresholds":
pass
elif opt in ('-c', "--mincluster"):
pass
elif opt == "--est-ref-size":
pass
elif opt == "--gene-thresholds":
pass
elif opt in ('-s', "--scaffolds"):
pass
elif opt == "--gage":
pass
elif opt == "--debug":
pass
elif opt in ('-e', "--eukaryote"):
pass
elif opt in ('-f', "--gene-finding"):
pass
elif opt in ('-i', "--min-alignment"):
pass
elif opt in ('-c', "--min-cluster"):
pass
elif opt in ('-a', "--ambiguity-usage"):
pass
elif opt in ('-u', "--use-all-alignments"):
pass
elif opt == "--strict-NA":
pass
elif opt in ('-x', "--extensive-mis-size"):
pass
elif opt == "--meta":
pass
elif opt == '--references-list':
ref_txt_fpath = arg
elif opt == '--glimmer':
pass
elif opt == '--no-snps':
pass
elif opt == '--no-check':
pass
elif opt == '--no-gc':
pass
elif opt == '--no-plots':
pass
elif opt == '--no-html':
html_report = False
elif opt == '--fast': # --no-check, --no-gc, --no-snps will automatically set in QUAST runs
html_report = False
elif opt == '--plots-format':
pass
elif opt == '--memory-efficient':
pass
elif opt == '--silent':
qconfig.silent = True
elif opt in ('-1', '--reads1'):
reads_fpath_f = arg
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
elif opt in ('-2', '--reads2'):
reads_fpath_r = arg
quast_py_args = __remove_from_quast_py_args(quast_py_args, opt, arg)
elif opt == '--contig-alignment-html':
qconfig.create_contig_alignment_html = True
else:
logger.error('Unknown option: %s. Use -h for help.' % (opt + ' ' + arg), to_stderr=True, exit_with_code=2)
for c_fpath in contigs_fpaths:
assert_file_exists(c_fpath, 'contigs')
labels = quast.process_labels(contigs_fpaths, labels, all_labels_from_dirs)
for contigs_fpath in contigs_fpaths:
if contigs_fpath in quast_py_args:
quast_py_args.remove(contigs_fpath)
# Directories
output_dirpath, _, _ = quast._set_up_output_dir(
output_dirpath, None, make_latest_symlink,
save_json=False)
corrected_dirpath = os.path.join(output_dirpath, qconfig.corrected_dirname)
logger.set_up_file_handler(output_dirpath)
args = [os.path.realpath(__file__)]
for k, v in options: args.extend([k, v])
args.extend(contigs_fpaths)
logger.print_command_line(args, wrap_after=None)
logger.start()
qconfig.set_max_threads(logger)
########################################################################
from libs import reporting
reload(reporting)
if os.path.isdir(corrected_dirpath):
shutil.rmtree(corrected_dirpath)
os.mkdir(corrected_dirpath)
# PROCESSING REFERENCES
if ref_fpaths:
logger.main_info()
logger.main_info('Reference(s):')
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(ref_fpaths, corrected_dirpath)
# PROCESSING CONTIGS
logger.main_info()
logger.main_info('Contigs:')
assemblies, correct_assemblies = _correct_contigs(contigs_fpaths, output_dirpath, labels)
if not assemblies:
logger.error("None of the assembly files contains correct contigs. "
"Please, provide different files or decrease --min-contig threshold.")
return 4
# Running QUAST(s)
quast_py_args += ['--meta']
downloaded_refs = False
# SEARCHING REFERENCES
if not ref_fpaths:
logger.main_info()
if qconfig.max_references == 0:
logger.notice("Maximum number of references (--max-ref-number) is set to 0, search in SILVA 16S rRNA database is disabled")
else:
if ref_txt_fpath:
logger.main_info("List of references was provided, starting to download reference genomes from NCBI...")
else:
logger.main_info("No references are provided, starting to search for reference genomes in SILVA 16S rRNA database "
"and to download them from NCBI...")
downloaded_dirpath = os.path.join(output_dirpath, qconfig.downloaded_dirname)
if not os.path.isdir(downloaded_dirpath):
os.mkdir(downloaded_dirpath)
ref_fpaths = search_references_meta.do(assemblies, labels, downloaded_dirpath, ref_txt_fpath)
if ref_fpaths:
search_references_meta.is_quast_first_run = True
if not ref_txt_fpath:
downloaded_refs = True
logger.main_info()
logger.main_info('Downloaded reference(s):')
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(ref_fpaths, corrected_dirpath)
elif test_mode and ref_fpaths is None:
logger.error('Failed to download or setup SILVA 16S rRNA database for working without '
'references on metagenome datasets!', to_stderr=True, exit_with_code=4)
if not ref_fpaths:
# No references, running regular quast with MetaGenemark gene finder
logger.main_info()
logger.notice('No references are provided, starting regular QUAST with MetaGeneMark gene finder')
_start_quast_main(
None,
quast_py_args,
assemblies=assemblies,
output_dirpath=output_dirpath,
exit_on_exception=True)
exit(0)
# Running combined reference
combined_output_dirpath = os.path.join(output_dirpath, qconfig.combined_output_name)
reads_fpaths = []
if reads_fpath_f:
reads_fpaths.append(reads_fpath_f)
if reads_fpath_r:
reads_fpaths.append(reads_fpath_r)
if reads_fpaths:
bed_fpath = reads_analyzer.do(combined_ref_fpath, contigs_fpaths, reads_fpaths, corrected_ref_fpaths,
os.path.join(combined_output_dirpath, qconfig.variation_dirname),
external_logger=logger)
if bed_fpath:
quast_py_args += ['--bed-file']
quast_py_args += [bed_fpath]
quast_py_args += ['--combined-ref']
run_name = 'for the combined reference'
logger.main_info()
logger.main_info('Starting quast.py ' + run_name + '...')
total_num_notices = 0
total_num_warnings = 0
total_num_nf_errors = 0
total_num_notifications = (total_num_notices, total_num_warnings, total_num_nf_errors)
if qconfig.html_report:
from libs.html_saver import json_saver
json_texts = []
else:
json_texts = None
return_code, total_num_notifications, assemblies, labels = _start_quast_main(run_name, quast_py_args + ["--ambiguity-usage"] + ['all'],
assemblies=assemblies,
reference_fpath=combined_ref_fpath,
output_dirpath=combined_output_dirpath,
num_notifications_tuple=total_num_notifications, is_first_run=True)
for arg in args:
if arg in ('-s', "--scaffolds"):
quast_py_args.remove(arg)
if json_texts is not None:
json_texts.append(json_saver.json_text)
search_references_meta.is_quast_first_run = False
genome_info_dirpath = os.path.join(output_dirpath, qconfig.combined_output_name, 'genome_stats')
genome_info_fpath = os.path.join(genome_info_dirpath, 'genome_info.txt')
if not os.path.exists(genome_info_fpath):
logger.main_info('')
logger.main_info('Failed aligning the contigs for all the references. ' + ('Try to restart MetaQUAST with another references.'
if not downloaded_refs else 'Try to use option --max-ref-number to change maximum number of references '
'(per each assembly) to download.'))
logger.main_info('')
quast._cleanup(corrected_dirpath)
logger.main_info('MetaQUAST finished.')
logger.finish_up(numbers=tuple(total_num_notifications), check_test=test_mode)
return
if downloaded_refs:
logger.main_info()
logger.main_info('Excluding downloaded references with low genome fraction from further analysis..')
corr_ref_fpaths = remove_unaligned_downloaded_refs(genome_info_fpath, ref_fpaths, chromosomes_by_refs)
if corr_ref_fpaths and corr_ref_fpaths != ref_fpaths:
logger.main_info()
logger.main_info('Filtered reference(s):')
os.remove(combined_ref_fpath)
contigs_analyzer.ref_labels_by_chromosomes = {}
corrected_ref_fpaths, combined_ref_fpath, chromosomes_by_refs, ref_names =\
_correct_references(corr_ref_fpaths, corrected_dirpath)
run_name = 'for the corrected combined reference'
logger.main_info()
logger.main_info('Starting quast.py ' + run_name + '...')
return_code, total_num_notifications, assemblies, labels = _start_quast_main(run_name, quast_py_args + ["--ambiguity-usage"] + ['all'],
assemblies=assemblies,
reference_fpath=combined_ref_fpath,
output_dirpath=combined_output_dirpath,
num_notifications_tuple=total_num_notifications, is_first_run=True)
if json_texts is not None:
json_texts = json_texts[:-1]
json_texts.append(json_saver.json_text)
elif corr_ref_fpaths == ref_fpaths:
logger.main_info('All downloaded references have genome fraction more than 10%. Nothing was excluded.')
else:
logger.main_info('All downloaded references have low genome fraction. Nothing was excluded for now.')
quast_py_args += ['--no-check-meta']
qconfig.contig_thresholds = ','.join([str(threshold) for threshold in qconfig.contig_thresholds if threshold > qconfig.min_contig])
if not qconfig.contig_thresholds:
qconfig.contig_thresholds = 'None'
quast_py_args = __remove_from_quast_py_args(quast_py_args, '--contig-thresholds', qconfig.contig_thresholds)
quast_py_args += ['--contig-thresholds']
quast_py_args += [qconfig.contig_thresholds]
quast_py_args.remove('--combined-ref')
logger.main_info()
logger.main_info('Partitioning contigs into bins aligned to each reference..')
assemblies_by_reference, not_aligned_assemblies = _partition_contigs(
assemblies, corrected_ref_fpaths, corrected_dirpath,
os.path.join(combined_output_dirpath, 'contigs_reports', 'alignments_%s.tsv'), labels)
ref_names = []
output_dirpath_per_ref = os.path.join(output_dirpath, qconfig.per_ref_dirname)
for ref_fpath, ref_assemblies in assemblies_by_reference:
ref_name = qutils.name_from_fpath(ref_fpath)
logger.main_info('')
if not ref_assemblies:
logger.main_info('No contigs were aligned to the reference ' + ref_name + ', skipping..')
else:
ref_names.append(ref_name)
run_name = 'for the contigs aligned to ' + ref_name
logger.main_info('Starting quast.py ' + run_name)
return_code, total_num_notifications = _start_quast_main(run_name, quast_py_args,
assemblies=ref_assemblies,
reference_fpath=ref_fpath,
output_dirpath=os.path.join(output_dirpath_per_ref, ref_name),
exit_on_exception=False, num_notifications_tuple=total_num_notifications)
if json_texts is not None:
json_texts.append(json_saver.json_text)
# Finally running for the contigs that has not been aligned to any reference
no_unaligned_contigs = True
for assembly in not_aligned_assemblies:
if os.path.isfile(assembly.fpath) and os.stat(assembly.fpath).st_size != 0:
no_unaligned_contigs = False
break
run_name = 'for the contigs not aligned anywhere'
logger.main_info()
if no_unaligned_contigs:
logger.main_info('Skipping quast.py ' + run_name + ' (everything is aligned!)')
else:
logger.main_info('Starting quast.py ' + run_name + '...')
return_code, total_num_notifications = _start_quast_main(run_name, quast_py_args,
assemblies=not_aligned_assemblies,
output_dirpath=os.path.join(output_dirpath, qconfig.not_aligned_name),
exit_on_exception=False, num_notifications_tuple=total_num_notifications)
if return_code not in [0, 4]:
logger.error('Error running quast.py for the contigs not aligned anywhere')
elif return_code == 4: # no unaligned contigs, i.e. everything aligned
no_unaligned_contigs = True
if not no_unaligned_contigs:
if json_texts is not None:
json_texts.append(json_saver.json_text)
if ref_names:
logger.print_timestamp()
logger.main_info("Summarizing results...")
summary_output_dirpath = os.path.join(output_dirpath, qconfig.meta_summary_dir)
if not os.path.isdir(summary_output_dirpath):
os.makedirs(summary_output_dirpath)
if html_report and json_texts:
from libs.html_saver import html_saver
html_summary_report_fpath = html_saver.init_meta_report(output_dirpath)
else:
html_summary_report_fpath = None
from libs import create_meta_summary
metrics_for_plots = reporting.Fields.main_metrics
misassembl_metrics = [reporting.Fields.MIS_RELOCATION, reporting.Fields.MIS_TRANSLOCATION, reporting.Fields.MIS_INVERTION,
reporting.Fields.MIS_ISTRANSLOCATIONS]
create_meta_summary.do(html_summary_report_fpath, summary_output_dirpath, combined_output_dirpath, output_dirpath_per_ref, metrics_for_plots, misassembl_metrics,
ref_names if no_unaligned_contigs else ref_names + [qconfig.not_aligned_name])
if html_report and json_texts:
from libs import plotter
html_saver.save_colors(output_dirpath, contigs_fpaths, plotter.dict_color_and_ls, meta=True)
html_saver.create_meta_report(output_dirpath, json_texts)
quast._cleanup(corrected_dirpath)
logger.main_info('')
logger.main_info('MetaQUAST finished.')
logger.finish_up(numbers=tuple(total_num_notifications), check_test=test_mode)
def main(args):
if ' ' in qconfig.QUAST_HOME:
logger.error(
'QUAST does not support spaces in paths. \n'
'You are trying to run it from ' + str(qconfig.QUAST_HOME) + '\n'
'Please, put QUAST in a different directory, then try again.\n',
to_stderr=True,
exit_with_code=3)
if not args:
qconfig.usage()
sys.exit(0)
reload(qconfig)
try:
options, contigs_fpaths = getopt.gnu_getopt(args,
qconfig.short_options,
qconfig.long_options)
except getopt.GetoptError:
_, exc_value, _ = sys.exc_info()
print >> sys.stderr, exc_value
print >> sys.stderr
qconfig.usage()
sys.exit(2)
for opt, arg in options[:]:
if opt == '--test' or opt == '--test-sv':
options.remove((opt, arg))
options += [
('-o', 'quast_test_output'),
('-R',
os.path.join(qconfig.QUAST_HOME, 'test_data',
'reference.fasta.gz')), # for compiling MUMmer
('-O',
os.path.join(qconfig.QUAST_HOME, 'test_data', 'operons.gff')),
('-G',
os.path.join(qconfig.QUAST_HOME, 'test_data', 'genes.gff')),
('--gage', ''), # for compiling GAGE Java classes
('--gene-finding', ''),
('--eukaryote', ''),
('--glimmer', '')
] # for compiling GlimmerHMM
if opt == '--test-sv':
options += [('-1',
os.path.join(qconfig.QUAST_HOME, 'test_data',
'reads1.fastq.gz')),
('-2',
os.path.join(qconfig.QUAST_HOME, 'test_data',
'reads2.fastq.gz'))]
contigs_fpaths += [
os.path.join(qconfig.QUAST_HOME, 'test_data',
'contigs_1.fasta'),
os.path.join(qconfig.QUAST_HOME, 'test_data',
'contigs_2.fasta')
]
qconfig.test = True
if opt.startswith('--help') or opt == '-h':
qconfig.usage(opt == "--help-hidden", short=False)
sys.exit(0)
elif opt.startswith('--version') or opt == '-v':
qconfig.print_version()
sys.exit(0)
if not contigs_fpaths:
logger.error("You should specify at least one file with contigs!\n")
qconfig.usage()
sys.exit(2)
json_output_dirpath = None
output_dirpath = None
labels = None
all_labels_from_dirs = False
qconfig.is_combined_ref = False
ref_fpath = ''
genes_fpaths = []
operons_fpaths = []
bed_fpath = None
reads_fpath_f = ''
reads_fpath_r = ''
# Yes, this is a code duplicating. But OptionParser is deprecated since version 2.7.
for opt, arg in options:
if opt in ('-d', '--debug'):
qconfig.debug = True
logger.set_up_console_handler(debug=True)
elif opt in ('-o', "--output-dir"):
output_dirpath = os.path.abspath(arg)
qconfig.make_latest_symlink = False
if ' ' in output_dirpath:
logger.error('QUAST does not support spaces in paths. \n'
'You have specified ' + str(output_dirpath) +
' as an output path.\n'
'Please, use a different directory.\n',
to_stderr=True,
exit_with_code=3)
elif opt in ('-G', "--genes"):
genes_fpaths.append(assert_file_exists(arg, 'genes'))
elif opt in ('-O', "--operons"):
operons_fpaths.append(assert_file_exists(arg, 'operons'))
elif opt in ('-R', "--reference"):
ref_fpath = assert_file_exists(arg, 'reference')
elif opt == "--contig-thresholds":
qconfig.contig_thresholds = arg
elif opt in ('-m', "--min-contig"):
qconfig.min_contig = int(arg)
elif opt in ('-t', "--threads"):
qconfig.max_threads = int(arg)
if qconfig.max_threads < 1:
qconfig.max_threads = 1
elif opt in ('-c', "--min-cluster"):
qconfig.min_cluster = int(arg)
elif opt in ('-i', "--min-alignment"):
qconfig.min_alignment = int(arg)
elif opt == "--est-ref-size":
qconfig.estimated_reference_size = int(arg)
elif opt == "--gene-thresholds":
qconfig.genes_lengths = arg
elif opt in ('-j', '--save-json'):
qconfig.save_json = True
elif opt in ('-J', '--save-json-to'):
qconfig.save_json = True
qconfig.make_latest_symlink = False
json_output_dirpath = arg
elif opt == '--err-fpath': # for web-quast
qconfig.save_error = True
qconfig.error_log_fname = arg
elif opt in ('-s', "--scaffolds"):
qconfig.scaffolds = True
elif opt == "--gage":
qconfig.with_gage = True
elif opt in ('-e', "--eukaryote"):
qconfig.prokaryote = False
elif opt in ('-f', "--gene-finding"):
qconfig.gene_finding = True
elif opt in ('-a', "--ambiguity-usage"):
if arg in ["none", "one", "all"]:
qconfig.ambiguity_usage = arg
elif opt in ('-u', "--use-all-alignments"):
qconfig.use_all_alignments = True
elif opt == "--strict-NA":
qconfig.strict_NA = True
elif opt in ('-x', "--extensive-mis-size"):
if int(arg) <= qconfig.MAX_INDEL_LENGTH:
logger.error(
"--extensive-mis-size should be greater than maximum indel length (%d)!"
% qconfig.MAX_INDEL_LENGTH,
1,
to_stderr=True)
qconfig.extensive_misassembly_threshold = int(arg)
elif opt == '--no-snps':
qconfig.show_snps = False
elif opt == '--no-plots':
qconfig.draw_plots = False
elif opt == '--no-html':
qconfig.html_report = False
elif opt == '--no-check':
qconfig.no_check = True
elif opt == '--no-gc':
qconfig.no_gc = True
elif opt == '--fast': # --no-gc, --no-plots, --no-snps
#qconfig.no_check = True # too risky to include
qconfig.no_gc = True
qconfig.show_snps = False
qconfig.draw_plots = False
qconfig.html_report = False
elif opt == '--plots-format':
if arg.lower() in qconfig.supported_plot_extensions:
qconfig.plot_extension = arg.lower()
else:
logger.error(
'Format "%s" is not supported. Please, use one of the supported formats: %s.'
% (arg, ', '.join(qconfig.supported_plot_extensions)),
to_stderr=True,
exit_with_code=2)
elif opt == '--meta':
qconfig.meta = True
elif opt == '--no-check-meta':
qconfig.no_check = True
qconfig.no_check_meta = True
elif opt == '--references-list':
pass
elif opt in ('-l', '--labels'):
labels = parse_labels(arg, contigs_fpaths)
elif opt == '-L':
all_labels_from_dirs = True
elif opt == '--glimmer':
qconfig.glimmer = True
elif opt == '--combined-ref':
qconfig.is_combined_ref = True
elif opt == '--memory-efficient':
qconfig.memory_efficient = True
elif opt == '--silent':
qconfig.silent = True
elif opt in ('-1', '--reads1'):
reads_fpath_f = arg
elif opt in ('-2', '--reads2'):
reads_fpath_r = arg
elif opt == '--bed-file':
bed_fpath = arg
elif opt == '--contig-alignment-html':
qconfig.create_contig_alignment_html = True
else:
logger.error('Unknown option: %s. Use -h for help.' %
(opt + ' ' + arg),
to_stderr=True,
exit_with_code=2)
for contigs_fpath in contigs_fpaths:
assert_file_exists(contigs_fpath, 'contigs')
labels = process_labels(contigs_fpaths, labels, all_labels_from_dirs)
output_dirpath, json_output_dirpath, existing_alignments = \
_set_up_output_dir(output_dirpath, json_output_dirpath, qconfig.make_latest_symlink, qconfig.save_json)
corrected_dirpath = os.path.join(output_dirpath, qconfig.corrected_dirname)
logger.set_up_file_handler(output_dirpath)
args = [os.path.realpath(__file__)]
for k, v in options:
args.extend([k, v])
args.extend(contigs_fpaths)
logger.print_command_line(args, wrap_after=None, is_main=True)
logger.start()
if existing_alignments:
logger.main_info()
logger.notice(
"Output directory already exists. Existing Nucmer alignments can be used."
)
qutils.remove_reports(output_dirpath)
if qconfig.contig_thresholds == "None":
qconfig.contig_thresholds = []
else:
qconfig.contig_thresholds = map(int,
qconfig.contig_thresholds.split(","))
if qconfig.genes_lengths == "None":
qconfig.genes_lengths = []
else:
qconfig.genes_lengths = map(int, qconfig.genes_lengths.split(","))
qconfig.set_max_threads(logger)
logger.main_info()
logger.print_params()
########################################################################
from libs import reporting
reload(reporting)
if qconfig.is_combined_ref:
corrected_dirpath = os.path.join(output_dirpath, '..',
qconfig.corrected_dirname)
else:
if os.path.isdir(corrected_dirpath):
shutil.rmtree(corrected_dirpath)
os.mkdir(corrected_dirpath)
# PROCESSING REFERENCE
if ref_fpath:
logger.main_info()
logger.main_info('Reference:')
ref_fpath = _correct_reference(ref_fpath, corrected_dirpath)
else:
ref_fpath = ''
# PROCESSING CONTIGS
logger.main_info()
logger.main_info('Contigs:')
contigs_fpaths, old_contigs_fpaths = _correct_contigs(
contigs_fpaths, corrected_dirpath, reporting, labels)
for contigs_fpath in contigs_fpaths:
report = reporting.get(contigs_fpath)
report.add_field(reporting.Fields.NAME,
qutils.label_from_fpath(contigs_fpath))
qconfig.assemblies_num = len(contigs_fpaths)
reads_fpaths = []
if reads_fpath_f:
reads_fpaths.append(reads_fpath_f)
if reads_fpath_r:
reads_fpaths.append(reads_fpath_r)
if reads_fpaths:
bed_fpath = reads_analyzer.do(ref_fpath,
contigs_fpaths,
reads_fpaths,
None,
os.path.join(output_dirpath,
qconfig.variation_dirname),
external_logger=logger)
if not contigs_fpaths:
logger.error(
"None of the assembly files contains correct contigs. "
"Please, provide different files or decrease --min-contig threshold.",
fake_if_nested_run=True)
return 4
qconfig.assemblies_fpaths = contigs_fpaths
if qconfig.with_gage:
########################################################################
### GAGE
########################################################################
if not ref_fpath:
logger.warning(
"GAGE can't be run without a reference and will be skipped.")
else:
from libs import gage
gage.do(ref_fpath, contigs_fpaths, output_dirpath)
# Where all pdfs will be saved
all_pdf_fpath = os.path.join(output_dirpath, qconfig.plots_fname)
all_pdf_file = None
if qconfig.draw_plots or qconfig.html_report:
from libs import plotter # Do not remove this line! It would lead to a warning in matplotlib.
try:
from matplotlib.backends.backend_pdf import PdfPages
all_pdf_file = PdfPages(all_pdf_fpath)
except:
all_pdf_file = None
if json_output_dirpath:
from libs.html_saver import json_saver
if json_saver.simplejson_error:
json_output_dirpath = None
########################################################################
### Stats and plots
########################################################################
from libs import basic_stats
basic_stats.do(ref_fpath, contigs_fpaths,
os.path.join(output_dirpath, 'basic_stats'),
json_output_dirpath, output_dirpath)
aligned_contigs_fpaths = []
aligned_lengths_lists = []
contig_alignment_plot_fpath = None
if ref_fpath:
########################################################################
### former PLANTAKOLYA, PLANTAGORA
########################################################################
from libs import contigs_analyzer
nucmer_statuses, aligned_lengths_per_fpath = contigs_analyzer.do(
ref_fpath, contigs_fpaths, qconfig.prokaryote,
os.path.join(output_dirpath, 'contigs_reports'),
old_contigs_fpaths, bed_fpath)
for contigs_fpath in contigs_fpaths:
if nucmer_statuses[
contigs_fpath] == contigs_analyzer.NucmerStatus.OK:
aligned_contigs_fpaths.append(contigs_fpath)
aligned_lengths_lists.append(
aligned_lengths_per_fpath[contigs_fpath])
# Before continue evaluating, check if nucmer didn't skip all of the contigs files.
detailed_contigs_reports_dirpath = None
if len(aligned_contigs_fpaths) and ref_fpath:
detailed_contigs_reports_dirpath = os.path.join(
output_dirpath, 'contigs_reports')
########################################################################
### NAx and NGAx ("aligned Nx and NGx")
########################################################################
from libs import aligned_stats
aligned_stats.do(ref_fpath, aligned_contigs_fpaths, output_dirpath,
json_output_dirpath, aligned_lengths_lists,
os.path.join(output_dirpath, 'aligned_stats'))
########################################################################
### GENOME_ANALYZER
########################################################################
from libs import genome_analyzer
genome_analyzer.do(ref_fpath, aligned_contigs_fpaths, output_dirpath,
json_output_dirpath, genes_fpaths, operons_fpaths,
detailed_contigs_reports_dirpath,
os.path.join(output_dirpath, 'genome_stats'))
if qconfig.gene_finding or qconfig.glimmer:
if qconfig.glimmer:
########################################################################
### Glimmer
########################################################################
from libs import glimmer
glimmer.do(contigs_fpaths, qconfig.genes_lengths,
os.path.join(output_dirpath, 'predicted_genes'))
else:
########################################################################
### GeneMark
########################################################################
from libs import genemark
genemark.do(contigs_fpaths, qconfig.genes_lengths,
os.path.join(output_dirpath, 'predicted_genes'),
qconfig.prokaryote, qconfig.meta)
else:
logger.main_info("")
logger.notice(
"Genes are not predicted by default. Use --gene-finding option to enable it."
)
########################################################################
reports_fpaths, transposed_reports_fpaths = reporting.save_total(
output_dirpath)
########################################################################
### LARGE DRAWING TASKS
########################################################################
if qconfig.draw_plots:
logger.print_timestamp()
logger.main_info('Drawing large plots...')
logger.main_info(
'This may take a while: press Ctrl-C to skip this step..')
try:
if detailed_contigs_reports_dirpath and qconfig.show_snps:
contig_report_fpath_pattern = os.path.join(
detailed_contigs_reports_dirpath,
'contigs_report_%s.stdout')
else:
contig_report_fpath_pattern = None
number_of_steps = sum([
int(bool(value))
for value in [contig_report_fpath_pattern, all_pdf_file]
])
if contig_report_fpath_pattern:
########################################################################
### VISUALIZE CONTIG ALIGNMENT
########################################################################
logger.main_info(
' 1 of %d: Creating contig alignment plot...' %
number_of_steps)
from libs import contig_alignment_plotter
contig_alignment_plot_fpath = contig_alignment_plotter.do(
contigs_fpaths,
contig_report_fpath_pattern,
output_dirpath,
ref_fpath,
similar=True)
if all_pdf_file:
# full report in PDF format: all tables and plots
logger.main_info(
' %d of %d: Creating PDF with all tables and plots...' %
(number_of_steps, number_of_steps))
plotter.fill_all_pdf_file(all_pdf_file)
logger.main_info('Done')
except KeyboardInterrupt:
logger.main_info('..step skipped!')
os.remove(all_pdf_fpath)
########################################################################
### TOTAL REPORT
########################################################################
logger.print_timestamp()
logger.main_info('RESULTS:')
logger.main_info(' Text versions of total report are saved to ' +
reports_fpaths)
logger.main_info(
' Text versions of transposed total report are saved to ' +
transposed_reports_fpaths)
if json_output_dirpath:
json_saver.save_total_report(json_output_dirpath, qconfig.min_contig,
ref_fpath)
if qconfig.html_report:
from libs.html_saver import html_saver
html_saver.save_colors(output_dirpath, contigs_fpaths,
plotter.dict_color_and_ls)
html_saver.save_total_report(output_dirpath, qconfig.min_contig,
ref_fpath)
if os.path.isfile(all_pdf_fpath):
logger.main_info(' PDF version (tables and plots) saved to ' +
all_pdf_fpath)
if contig_alignment_plot_fpath:
logger.main_info(' Contig alignment plot: %s' %
contig_alignment_plot_fpath)
_cleanup(corrected_dirpath)
logger.finish_up(check_test=qconfig.test)
return 0
