def main(args=None): # Initialization param = Param() start_time = time.time() parser = get_parser() arguments = parser.parse_args(args=None if sys.argv[1:] else ['--help']) fname_anat = arguments.i fname_centerline = arguments.s param.algo_fitting = arguments.algo_fitting if arguments.smooth is not None: sigma = arguments.smooth remove_temp_files = arguments.r verbose = int(arguments.v) init_sct(log_level=verbose, update=True) # Update log level # Display arguments printv('\nCheck input arguments...') printv(' Volume to smooth .................. ' + fname_anat) printv(' Centerline ........................ ' + fname_centerline) printv(' Sigma (mm) ........................ ' + str(sigma)) printv(' Verbose ........................... ' + str(verbose)) # Check that input is 3D: nx, ny, nz, nt, px, py, pz, pt = Image(fname_anat).dim dim = 4 # by default, will be adjusted later if nt == 1: dim = 3 if nz == 1: dim = 2 if dim == 4: printv('WARNING: the input image is 4D, please split your image to 3D before smoothing spinalcord using :\n' 'sct_image -i ' + fname_anat + ' -split t -o ' + fname_anat, verbose, 'warning') printv('4D images not supported, aborting ...', verbose, 'error') # Extract path/file/extension path_anat, file_anat, ext_anat = extract_fname(fname_anat) path_centerline, file_centerline, ext_centerline = extract_fname(fname_centerline) path_tmp = tmp_create(basename="smooth_spinalcord") # Copying input data to tmp folder printv('\nCopying input data to tmp folder and convert to nii...', verbose) copy(fname_anat, os.path.join(path_tmp, "anat" + ext_anat)) copy(fname_centerline, os.path.join(path_tmp, "centerline" + ext_centerline)) # go to tmp folder curdir = os.getcwd() os.chdir(path_tmp) # convert to nii format convert('anat' + ext_anat, 'anat.nii') convert('centerline' + ext_centerline, 'centerline.nii') # Change orientation of the input image into RPI printv('\nOrient input volume to RPI orientation...') fname_anat_rpi = Image("anat.nii") \ .change_orientation("RPI", generate_path=True) \ .save() \ .absolutepath # Change orientation of the input image into RPI printv('\nOrient centerline to RPI orientation...') fname_centerline_rpi = Image("centerline.nii") \ .change_orientation("RPI", generate_path=True) \ .save() \ .absolutepath # Straighten the spinal cord # straighten segmentation printv('\nStraighten the spinal cord using centerline/segmentation...', verbose) cache_sig = cache_signature(input_files=[fname_anat_rpi, fname_centerline_rpi], input_params={"x": "spline"}) cachefile = os.path.join(curdir, "straightening.cache") if cache_valid(cachefile, cache_sig) and os.path.isfile(os.path.join(curdir, 'warp_curve2straight.nii.gz')) and os.path.isfile(os.path.join(curdir, 'warp_straight2curve.nii.gz')) and os.path.isfile(os.path.join(curdir, 'straight_ref.nii.gz')): # if they exist, copy them into current folder printv('Reusing existing warping field which seems to be valid', verbose, 'warning') copy(os.path.join(curdir, 'warp_curve2straight.nii.gz'), 'warp_curve2straight.nii.gz') copy(os.path.join(curdir, 'warp_straight2curve.nii.gz'), 'warp_straight2curve.nii.gz') copy(os.path.join(curdir, 'straight_ref.nii.gz'), 'straight_ref.nii.gz') # apply straightening run_proc(['sct_apply_transfo', '-i', fname_anat_rpi, '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', 'anat_rpi_straight.nii', '-x', 'spline'], verbose) else: run_proc(['sct_straighten_spinalcord', '-i', fname_anat_rpi, '-o', 'anat_rpi_straight.nii', '-s', fname_centerline_rpi, '-x', 'spline', '-param', 'algo_fitting=' + param.algo_fitting], verbose) cache_save(cachefile, cache_sig) # move warping fields locally (to use caching next time) copy('warp_curve2straight.nii.gz', os.path.join(curdir, 'warp_curve2straight.nii.gz')) copy('warp_straight2curve.nii.gz', os.path.join(curdir, 'warp_straight2curve.nii.gz')) # Smooth the straightened image along z printv('\nSmooth the straightened image...') sigma_smooth = ",".join([str(i) for i in sigma]) sct_maths.main(args=['-i', 'anat_rpi_straight.nii', '-smooth', sigma_smooth, '-o', 'anat_rpi_straight_smooth.nii', '-v', '0']) # Apply the reversed warping field to get back the curved spinal cord printv('\nApply the reversed warping field to get back the curved spinal cord...') run_proc(['sct_apply_transfo', '-i', 'anat_rpi_straight_smooth.nii', '-o', 'anat_rpi_straight_smooth_curved.nii', '-d', 'anat.nii', '-w', 'warp_straight2curve.nii.gz', '-x', 'spline'], verbose) # replace zeroed voxels by original image (issue #937) printv('\nReplace zeroed voxels by original image...', verbose) nii_smooth = Image('anat_rpi_straight_smooth_curved.nii') data_smooth = nii_smooth.data data_input = Image('anat.nii').data indzero = np.where(data_smooth == 0) data_smooth[indzero] = data_input[indzero] nii_smooth.data = data_smooth nii_smooth.save('anat_rpi_straight_smooth_curved_nonzero.nii') # come back os.chdir(curdir) # Generate output file printv('\nGenerate output file...') generate_output_file(os.path.join(path_tmp, "anat_rpi_straight_smooth_curved_nonzero.nii"), file_anat + '_smooth' + ext_anat) # Remove temporary files if remove_temp_files == 1: printv('\nRemove temporary files...') rmtree(path_tmp) # Display elapsed time elapsed_time = time.time() - start_time printv('\nFinished! Elapsed time: ' + str(int(np.round(elapsed_time))) + 's\n') display_viewer_syntax([file_anat, file_anat + '_smooth'], verbose=verbose)
def compute_texture(self): offset = int(self.param_glcm.distance) printv('\nCompute texture metrics...', self.param.verbose, 'normal') # open image and re-orient it to RPI if needed im_tmp = Image(self.param.fname_im) if self.orientation_im != self.orientation_extraction: im_tmp.change_orientation(self.orientation_extraction) dct_metric = {} for m in self.metric_lst: im_2save = zeros_like(im_tmp, dtype='float64') dct_metric[m] = im_2save # dct_metric[m] = Image(self.fname_metric_lst[m]) with sct_progress_bar() as pbar: for im_z, seg_z, zz in zip(self.dct_im_seg['im'], self.dct_im_seg['seg'], range(len(self.dct_im_seg['im']))): for xx in range(im_z.shape[0]): for yy in range(im_z.shape[1]): if not seg_z[xx, yy]: continue if xx < offset or yy < offset: continue if xx > (im_z.shape[0] - offset - 1) or yy > (im_z.shape[1] - offset - 1): continue # to check if the whole glcm_window is in the axial_slice if False in np.unique( seg_z[xx - offset:xx + offset + 1, yy - offset:yy + offset + 1]): continue # to check if the whole glcm_window is in the mask of the axial_slice glcm_window = im_z[xx - offset:xx + offset + 1, yy - offset:yy + offset + 1] glcm_window = glcm_window.astype(np.uint8) dct_glcm = {} for a in self.param_glcm.angle.split( ',' ): # compute the GLCM for self.param_glcm.distance and for each self.param_glcm.angle dct_glcm[a] = greycomatrix( glcm_window, [self.param_glcm.distance], [np.radians(int(a))], symmetric=self.param_glcm.symmetric, normed=self.param_glcm.normed) for m in self.metric_lst: # compute the GLCM property (m.split('_')[0]) of the voxel xx,yy,zz dct_metric[m].data[xx, yy, zz] = greycoprops( dct_glcm[m.split('_')[2]], m.split('_')[0])[0][0] pbar.set_postfix( pos="{}/{}".format(zz, len(self.dct_im_seg["im"]))) pbar.update(1) for m in self.metric_lst: fname_out = add_suffix( "".join(extract_fname(self.param.fname_im)[1:]), '_' + m) dct_metric[m].save(fname_out) self.fname_metric_lst[m] = fname_out
def create_mask(param): # parse argument for method method_type = param.process[0] # check method val if not method_type == 'center': method_val = param.process[1] # check existence of input files if method_type == 'centerline': check_file_exist(method_val, param.verbose) # Extract path/file/extension path_data, file_data, ext_data = extract_fname(param.fname_data) # Get output folder and file name if param.fname_out == '': param.fname_out = os.path.abspath(param.file_prefix + file_data + ext_data) path_tmp = tmp_create(basename="create_mask") printv('\nOrientation:', param.verbose) orientation_input = Image(param.fname_data).orientation printv(' ' + orientation_input, param.verbose) # copy input data to tmp folder and re-orient to RPI Image(param.fname_data).change_orientation("RPI").save( os.path.join(path_tmp, "data_RPI.nii")) if method_type == 'centerline': Image(method_val).change_orientation("RPI").save( os.path.join(path_tmp, "centerline_RPI.nii")) if method_type == 'point': Image(method_val).change_orientation("RPI").save( os.path.join(path_tmp, "point_RPI.nii")) # go to tmp folder curdir = os.getcwd() os.chdir(path_tmp) # Get dimensions of data im_data = Image('data_RPI.nii') nx, ny, nz, nt, px, py, pz, pt = im_data.dim printv('\nDimensions:', param.verbose) printv(im_data.dim, param.verbose) # in case user input 4d data if nt != 1: printv( 'WARNING in ' + os.path.basename(__file__) + ': Input image is 4d but output mask will be 3D from first time slice.', param.verbose, 'warning') # extract first volume to have 3d reference nii = empty_like(Image('data_RPI.nii')) data3d = nii.data[:, :, :, 0] nii.data = data3d nii.save('data_RPI.nii') if method_type == 'coord': # parse to get coordinate coord = [x for x in map(int, method_val.split('x'))] if method_type == 'point': # extract coordinate of point printv('\nExtract coordinate of point...', param.verbose) coord = Image("point_RPI.nii").getNonZeroCoordinates() if method_type == 'center': # set coordinate at center of FOV coord = np.round(float(nx) / 2), np.round(float(ny) / 2) if method_type == 'centerline': # get name of centerline from user argument fname_centerline = 'centerline_RPI.nii' else: # generate volume with line along Z at coordinates 'coord' printv('\nCreate line...', param.verbose) fname_centerline = create_line(param, 'data_RPI.nii', coord, nz) # create mask printv('\nCreate mask...', param.verbose) centerline = nibabel.load(fname_centerline) # open centerline hdr = centerline.get_header() # get header hdr.set_data_dtype('uint8') # set imagetype to uint8 spacing = hdr.structarr['pixdim'] data_centerline = centerline.get_data() # get centerline # if data is 2D, reshape with empty third dimension if len(data_centerline.shape) == 2: data_centerline_shape = list(data_centerline.shape) data_centerline_shape.append(1) data_centerline = data_centerline.reshape(data_centerline_shape) z_centerline_not_null = [ iz for iz in range(0, nz, 1) if data_centerline[:, :, iz].any() ] # get center of mass of the centerline cx = [0] * nz cy = [0] * nz for iz in range(0, nz, 1): if iz in z_centerline_not_null: cx[iz], cy[iz] = ndimage.measurements.center_of_mass( np.array(data_centerline[:, :, iz])) # create 2d masks file_mask = 'data_mask' for iz in range(nz): if iz not in z_centerline_not_null: # write an empty nifty volume img = nibabel.Nifti1Image(data_centerline[:, :, iz], None, hdr) nibabel.save(img, (file_mask + str(iz) + '.nii')) else: center = np.array([cx[iz], cy[iz]]) mask2d = create_mask2d(param, center, param.shape, param.size, im_data=im_data) # Write NIFTI volumes img = nibabel.Nifti1Image(mask2d, None, hdr) nibabel.save(img, (file_mask + str(iz) + '.nii')) fname_list = [file_mask + str(iz) + '.nii' for iz in range(nz)] im_out = concat_data(fname_list, dim=2).save('mask_RPI.nii.gz') im_out.change_orientation(orientation_input) im_out.header = Image(param.fname_data).header im_out.save(param.fname_out) # come back os.chdir(curdir) # Remove temporary files if param.remove_temp_files == 1: printv('\nRemove temporary files...', param.verbose) rmtree(path_tmp) display_viewer_syntax([param.fname_data, param.fname_out], colormaps=['gray', 'red'], opacities=['', '0.5'])
def main(argv=None): parser = get_parser() arguments = parser.parse_args(argv) verbose = arguments.v set_loglevel(verbose=verbose) # Input filename fname_input_data = arguments.i fname_data = os.path.abspath(fname_input_data) # Method used method = arguments.method # Contrast type contrast_type = arguments.c if method == 'optic' and not contrast_type: # Contrast must be error = "ERROR: -c is a mandatory argument when using 'optic' method." printv(error, type='error') return # Gap between slices interslice_gap = arguments.gap param_centerline = ParamCenterline(algo_fitting=arguments.centerline_algo, smooth=arguments.centerline_smooth, minmax=True) # Output folder if arguments.o is not None: path_data, file_data, ext_data = extract_fname(arguments.o) if not ext_data: ext_data = '.nii.gz' file_output = os.path.join(path_data, file_data + ext_data) else: path_data, file_data, ext_data = extract_fname(fname_data) file_output = os.path.join(path_data, file_data + '_centerline.nii.gz') if method == 'viewer': # Manual labeling of cord centerline im_labels = _call_viewer_centerline(Image(fname_data), interslice_gap=interslice_gap) elif method == 'fitseg': im_labels = Image(fname_data) elif method == 'optic': # Automatic detection of cord centerline im_labels = Image(fname_data) param_centerline.algo_fitting = 'optic' param_centerline.contrast = contrast_type else: printv( "ERROR: The selected method is not available: {}. Please look at the help." .format(method), type='error') return # Extrapolate and regularize (or detect if optic) cord centerline im_centerline, arr_centerline, _, _ = get_centerline( im_labels, param=param_centerline, verbose=verbose) # save centerline as nifti (discrete) and csv (continuous) files im_centerline.save(file_output) np.savetxt(file_output + '.csv', arr_centerline.transpose(), delimiter=",") path_qc = arguments.qc qc_dataset = arguments.qc_dataset qc_subject = arguments.qc_subject # Generate QC report if path_qc is not None: generate_qc(fname_input_data, fname_seg=file_output, args=sys.argv[1:], path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject, process='sct_get_centerline') display_viewer_syntax([fname_input_data, file_output], colormaps=['gray', 'red'], opacities=['', '0.7'])
def main(argv=None): parser = get_parser() arguments = parser.parse_args(argv) verbose = arguments.v set_loglevel(verbose=verbose) fname_in = os.path.abspath(arguments.i) fname_seg = os.path.abspath(arguments.s) contrast = arguments.c path_template = os.path.abspath(arguments.t) scale_dist = arguments.scale_dist path_output = os.path.abspath(arguments.ofolder) fname_disc = arguments.discfile if fname_disc is not None: fname_disc = os.path.abspath(fname_disc) initz = arguments.initz initcenter = arguments.initcenter fname_initlabel = arguments.initlabel if fname_initlabel is not None: fname_initlabel = os.path.abspath(fname_initlabel) remove_temp_files = arguments.r clean_labels = arguments.clean_labels path_tmp = tmp_create(basename="label_vertebrae") # Copying input data to tmp folder printv('\nCopying input data to tmp folder...', verbose) Image(fname_in).save(os.path.join(path_tmp, "data.nii")) Image(fname_seg).save(os.path.join(path_tmp, "segmentation.nii")) # Go go temp folder curdir = os.getcwd() os.chdir(path_tmp) # Straighten spinal cord printv('\nStraighten spinal cord...', verbose) # check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time) cache_sig = cache_signature(input_files=[fname_in, fname_seg], ) fname_cache = "straightening.cache" if (cache_valid(os.path.join(curdir, fname_cache), cache_sig) and os.path.isfile( os.path.join(curdir, "warp_curve2straight.nii.gz")) and os.path.isfile( os.path.join(curdir, "warp_straight2curve.nii.gz")) and os.path.isfile(os.path.join(curdir, "straight_ref.nii.gz"))): # if they exist, copy them into current folder printv('Reusing existing warping field which seems to be valid', verbose, 'warning') copy(os.path.join(curdir, "warp_curve2straight.nii.gz"), 'warp_curve2straight.nii.gz') copy(os.path.join(curdir, "warp_straight2curve.nii.gz"), 'warp_straight2curve.nii.gz') copy(os.path.join(curdir, "straight_ref.nii.gz"), 'straight_ref.nii.gz') # apply straightening s, o = run_proc([ 'sct_apply_transfo', '-i', 'data.nii', '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', 'data_straight.nii' ]) else: sct_straighten_spinalcord.main(argv=[ '-i', 'data.nii', '-s', 'segmentation.nii', '-r', str(remove_temp_files), '-v', '0', ]) cache_save(os.path.join(path_output, fname_cache), cache_sig) # resample to 0.5mm isotropic to match template resolution printv('\nResample to 0.5mm isotropic...', verbose) s, o = run_proc([ 'sct_resample', '-i', 'data_straight.nii', '-mm', '0.5x0.5x0.5', '-x', 'linear', '-o', 'data_straightr.nii' ], verbose=verbose) # Apply straightening to segmentation # N.B. Output is RPI printv('\nApply straightening to segmentation...', verbose) sct_apply_transfo.main([ '-i', 'segmentation.nii', '-d', 'data_straightr.nii', '-w', 'warp_curve2straight.nii.gz', '-o', 'segmentation_straight.nii', '-x', 'linear', '-v', '0' ]) # Threshold segmentation at 0.5 img = Image('segmentation_straight.nii') img.data = threshold(img.data, 0.5) img.save() # If disc label file is provided, label vertebrae using that file instead of automatically if fname_disc: # Apply straightening to disc-label printv('\nApply straightening to disc labels...', verbose) run_proc( 'sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' % (fname_disc, 'data_straightr.nii', 'warp_curve2straight.nii.gz', 'labeldisc_straight.nii.gz', 'label'), verbose=verbose) label_vert('segmentation_straight.nii', 'labeldisc_straight.nii.gz', verbose=1) else: printv('\nCreate label to identify disc...', verbose) fname_labelz = os.path.join(path_tmp, 'labelz.nii.gz') if initcenter is not None: # find z centered in FOV nii = Image('segmentation.nii').change_orientation("RPI") nx, ny, nz, nt, px, py, pz, pt = nii.dim z_center = round(nz / 2) initz = [z_center, initcenter] if initz is not None: im_label = create_labels_along_segmentation( Image('segmentation.nii'), [tuple(initz)]) im_label.save(fname_labelz) elif fname_initlabel is not None: Image(fname_initlabel).save(fname_labelz) else: # automatically finds C2-C3 disc im_data = Image('data.nii') im_seg = Image('segmentation.nii') # because verbose is also used for keeping temp files verbose_detect_c2c3 = 0 if remove_temp_files else 2 im_label_c2c3 = detect_c2c3(im_data, im_seg, contrast, verbose=verbose_detect_c2c3) ind_label = np.where(im_label_c2c3.data) if np.size(ind_label) == 0: printv( 'Automatic C2-C3 detection failed. Please provide manual label with sct_label_utils', 1, 'error') sys.exit(1) im_label_c2c3.data[ind_label] = 3 im_label_c2c3.save(fname_labelz) # dilate label so it is not lost when applying warping dilate(Image(fname_labelz), 3, 'ball').save(fname_labelz) # Apply straightening to z-label printv('\nAnd apply straightening to label...', verbose) sct_apply_transfo.main([ '-i', 'labelz.nii.gz', '-d', 'data_straightr.nii', '-w', 'warp_curve2straight.nii.gz', '-o', 'labelz_straight.nii.gz', '-x', 'nn', '-v', '0' ]) # get z value and disk value to initialize labeling printv('\nGet z and disc values from straight label...', verbose) init_disc = get_z_and_disc_values_from_label('labelz_straight.nii.gz') printv('.. ' + str(init_disc), verbose) # apply laplacian filtering if arguments.laplacian: printv('\nApply Laplacian filter...', verbose) img = Image("data_straightr.nii") # apply std dev to each axis of the image sigmas = [1 for i in range(len(img.data.shape))] # adjust sigma based on voxel size sigmas = [sigmas[i] / img.dim[i + 4] for i in range(3)] # smooth data img.data = laplacian(img.data, sigmas) img.save() # detect vertebral levels on straight spinal cord init_disc[1] = init_disc[1] - 1 vertebral_detection('data_straightr.nii', 'segmentation_straight.nii', contrast, arguments.param, init_disc=init_disc, verbose=verbose, path_template=path_template, path_output=path_output, scale_dist=scale_dist) # un-straighten labeled spinal cord printv('\nUn-straighten labeling...', verbose) sct_apply_transfo.main([ '-i', 'segmentation_straight_labeled.nii', '-d', 'segmentation.nii', '-w', 'warp_straight2curve.nii.gz', '-o', 'segmentation_labeled.nii', '-x', 'nn', '-v', '0' ]) if clean_labels >= 1: printv('\nCleaning labeled segmentation:', verbose) im_labeled_seg = Image('segmentation_labeled.nii') im_seg = Image('segmentation.nii') if clean_labels >= 2: printv(' filling in missing label voxels ...', verbose) expand_labels(im_labeled_seg) printv(' removing labeled voxels outside segmentation...', verbose) crop_labels(im_labeled_seg, im_seg) printv('Done cleaning.', verbose) im_labeled_seg.save() # label discs printv('\nLabel discs...', verbose) printv('\nUn-straighten labeled discs...', verbose) run_proc( 'sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' % ('segmentation_straight_labeled_disc.nii', 'segmentation.nii', 'warp_straight2curve.nii.gz', 'segmentation_labeled_disc.nii', 'label'), verbose=verbose, is_sct_binary=True, ) # come back os.chdir(curdir) # Generate output files path_seg, file_seg, ext_seg = extract_fname(fname_seg) fname_seg_labeled = os.path.join(path_output, file_seg + '_labeled' + ext_seg) printv('\nGenerate output files...', verbose) generate_output_file(os.path.join(path_tmp, "segmentation_labeled.nii"), fname_seg_labeled) generate_output_file( os.path.join(path_tmp, "segmentation_labeled_disc.nii"), os.path.join(path_output, file_seg + '_labeled_discs' + ext_seg)) # copy straightening files in case subsequent SCT functions need them generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose=verbose) generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose=verbose) generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose=verbose) # Remove temporary files if remove_temp_files == 1: printv('\nRemove temporary files...', verbose) rmtree(path_tmp) # Generate QC report if arguments.qc is not None: path_qc = os.path.abspath(arguments.qc) qc_dataset = arguments.qc_dataset qc_subject = arguments.qc_subject labeled_seg_file = os.path.join(path_output, file_seg + '_labeled' + ext_seg) generate_qc(fname_in, fname_seg=labeled_seg_file, args=argv, path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject, process='sct_label_vertebrae') display_viewer_syntax([fname_in, fname_seg_labeled], colormaps=['', 'subcortical'], opacities=['1', '0.5'])
def apply(self): # Initialization fname_src = self.input_filename # source image (moving) list_warp = self.list_warp # list of warping fields fname_out = self.output_filename # output fname_dest = self.fname_dest # destination image (fix) verbose = self.verbose remove_temp_files = self.remove_temp_files crop_reference = self.crop # if = 1, put 0 everywhere around warping field, if = 2, real crop islabel = False if self.interp == 'label': islabel = True self.interp = 'nn' interp = get_interpolation('isct_antsApplyTransforms', self.interp) # Parse list of warping fields printv('\nParse list of warping fields...', verbose) use_inverse = [] fname_warp_list_invert = [] # list_warp = list_warp.replace(' ', '') # remove spaces # list_warp = list_warp.split(",") # parse with comma for idx_warp, path_warp in enumerate(self.list_warp): # Check if this transformation should be inverted if path_warp in self.list_warpinv: use_inverse.append('-i') # list_warp[idx_warp] = path_warp[1:] # remove '-' fname_warp_list_invert += [[ use_inverse[idx_warp], list_warp[idx_warp] ]] else: use_inverse.append('') fname_warp_list_invert += [[path_warp]] path_warp = list_warp[idx_warp] if path_warp.endswith((".nii", ".nii.gz")) \ and Image(list_warp[idx_warp]).header.get_intent()[0] != 'vector': raise ValueError( "Displacement field in {} is invalid: should be encoded" " in a 5D file with vector intent code" " (see https://nifti.nimh.nih.gov/pub/dist/src/niftilib/nifti1.h" .format(path_warp)) # need to check if last warping field is an affine transfo isLastAffine = False path_fname, file_fname, ext_fname = extract_fname( fname_warp_list_invert[-1][-1]) if ext_fname in ['.txt', '.mat']: isLastAffine = True # check if destination file is 3d # check_dim(fname_dest, dim_lst=[3]) # PR 2598: we decided to skip this line. # N.B. Here we take the inverse of the warp list, because sct_WarpImageMultiTransform concatenates in the reverse order fname_warp_list_invert.reverse() fname_warp_list_invert = functools.reduce(lambda x, y: x + y, fname_warp_list_invert) # Extract path, file and extension path_src, file_src, ext_src = extract_fname(fname_src) path_dest, file_dest, ext_dest = extract_fname(fname_dest) # Get output folder and file name if fname_out == '': path_out = '' # output in user's current directory file_out = file_src + '_reg' ext_out = ext_src fname_out = os.path.join(path_out, file_out + ext_out) # Get dimensions of data printv('\nGet dimensions of data...', verbose) img_src = Image(fname_src) nx, ny, nz, nt, px, py, pz, pt = img_src.dim # nx, ny, nz, nt, px, py, pz, pt = get_dimension(fname_src) printv( ' ' + str(nx) + ' x ' + str(ny) + ' x ' + str(nz) + ' x ' + str(nt), verbose) # if 3d if nt == 1: # Apply transformation printv('\nApply transformation...', verbose) if nz in [0, 1]: dim = '2' else: dim = '3' # if labels, dilate before resampling if islabel: printv("\nDilate labels before warping...") path_tmp = tmp_create(basename="apply_transfo") fname_dilated_labels = os.path.join(path_tmp, "dilated_data.nii") # dilate points dilate(Image(fname_src), 4, 'ball').save(fname_dilated_labels) fname_src = fname_dilated_labels printv( "\nApply transformation and resample to destination space...", verbose) run_proc([ 'isct_antsApplyTransforms', '-d', dim, '-i', fname_src, '-o', fname_out, '-t' ] + fname_warp_list_invert + ['-r', fname_dest] + interp, is_sct_binary=True) # if 4d, loop across the T dimension else: if islabel: raise NotImplementedError dim = '4' path_tmp = tmp_create(basename="apply_transfo") # convert to nifti into temp folder printv('\nCopying input data to tmp folder and convert to nii...', verbose) img_src.save(os.path.join(path_tmp, "data.nii")) copy(fname_dest, os.path.join(path_tmp, file_dest + ext_dest)) fname_warp_list_tmp = [] for fname_warp in list_warp: path_warp, file_warp, ext_warp = extract_fname(fname_warp) copy(fname_warp, os.path.join(path_tmp, file_warp + ext_warp)) fname_warp_list_tmp.append(file_warp + ext_warp) fname_warp_list_invert_tmp = fname_warp_list_tmp[::-1] curdir = os.getcwd() os.chdir(path_tmp) # split along T dimension printv('\nSplit along T dimension...', verbose) im_dat = Image('data.nii') im_header = im_dat.hdr data_split_list = sct_image.split_data(im_dat, 3) for im in data_split_list: im.save() # apply transfo printv('\nApply transformation to each 3D volume...', verbose) for it in range(nt): file_data_split = 'data_T' + str(it).zfill(4) + '.nii' file_data_split_reg = 'data_reg_T' + str(it).zfill(4) + '.nii' status, output = run_proc([ 'isct_antsApplyTransforms', '-d', '3', '-i', file_data_split, '-o', file_data_split_reg, '-t', ] + fname_warp_list_invert_tmp + [ '-r', file_dest + ext_dest, ] + interp, verbose, is_sct_binary=True) # Merge files back printv('\nMerge file back...', verbose) import glob path_out, name_out, ext_out = extract_fname(fname_out) # im_list = [Image(file_name) for file_name in glob.glob('data_reg_T*.nii')] # concat_data use to take a list of image in input, now takes a list of file names to open the files one by one (see issue #715) fname_list = glob.glob('data_reg_T*.nii') fname_list.sort() im_list = [Image(fname) for fname in fname_list] im_out = sct_image.concat_data(im_list, 3, im_header['pixdim']) im_out.save(name_out + ext_out) os.chdir(curdir) generate_output_file(os.path.join(path_tmp, name_out + ext_out), fname_out) # Delete temporary folder if specified if remove_temp_files: printv('\nRemove temporary files...', verbose) rmtree(path_tmp, verbose=verbose) # Copy affine matrix from destination space to make sure qform/sform are the same printv( "Copy affine matrix from destination space to make sure qform/sform are the same.", verbose) im_src_reg = Image(fname_out) im_src_reg.copy_qform_from_ref(Image(fname_dest)) im_src_reg.save( verbose=0 ) # set verbose=0 to avoid warning message about rewriting file if islabel: printv( "\nTake the center of mass of each registered dilated labels..." ) labeled_img = cubic_to_point(im_src_reg) labeled_img.save(path=fname_out) if remove_temp_files: printv('\nRemove temporary files...', verbose) rmtree(path_tmp, verbose=verbose) # Crop the resulting image using dimensions from the warping field warping_field = fname_warp_list_invert[-1] # If the last transformation is not an affine transfo, we need to compute the matrix space of the concatenated # warping field if not isLastAffine and crop_reference in [1, 2]: printv('Last transformation is not affine.') if crop_reference in [1, 2]: # Extract only the first ndim of the warping field img_warp = Image(warping_field) if dim == '2': img_warp_ndim = Image(img_src.data[:, :], hdr=img_warp.hdr) elif dim in ['3', '4']: img_warp_ndim = Image(img_src.data[:, :, :], hdr=img_warp.hdr) # Set zero to everything outside the warping field cropper = ImageCropper(Image(fname_out)) cropper.get_bbox_from_ref(img_warp_ndim) if crop_reference == 1: printv( 'Cropping strategy is: keep same matrix size, put 0 everywhere around warping field' ) img_out = cropper.crop(background=0) elif crop_reference == 2: printv( 'Cropping strategy is: crop around warping field (the size of warping field will ' 'change)') img_out = cropper.crop() img_out.save(fname_out) display_viewer_syntax([fname_dest, fname_out], verbose=verbose)
def straighten(self): """ Straighten spinal cord. Steps: (everything is done in physical space) 1. open input image and centreline image 2. extract bspline fitting of the centreline, and its derivatives 3. compute length of centerline 4. compute and generate straight space 5. compute transformations for each voxel of one space: (done using matrices --> improves speed by a factor x300) a. determine which plane of spinal cord centreline it is included b. compute the position of the voxel in the plane (X and Y distance from centreline, along the plane) c. find the correspondant centreline point in the other space d. find the correspondance of the voxel in the corresponding plane 6. generate warping fields for each transformations 7. write warping fields and apply them step 5.b: how to find the corresponding plane? The centerline plane corresponding to a voxel correspond to the nearest point of the centerline. However, we need to compute the distance between the voxel position and the plane to be sure it is part of the plane and not too distant. If it is more far than a threshold, warping value should be 0. step 5.d: how to make the correspondance between centerline point in both images? Both centerline have the same lenght. Therefore, we can map centerline point via their position along the curve. If we use the same number of points uniformely along the spinal cord (1000 for example), the correspondance is straight-forward. :return: """ # Initialization fname_anat = self.input_filename fname_centerline = self.centerline_filename fname_output = self.output_filename remove_temp_files = self.remove_temp_files verbose = self.verbose interpolation_warp = self.interpolation_warp # TODO: remove this # start timer start_time = time.time() # Extract path/file/extension path_anat, file_anat, ext_anat = extract_fname(fname_anat) path_tmp = tmp_create(basename="straighten_spinalcord") # Copying input data to tmp folder logger.info('Copy files to tmp folder...') Image(fname_anat).save(os.path.join(path_tmp, "data.nii")) Image(fname_centerline).save(os.path.join(path_tmp, "centerline.nii.gz")) if self.use_straight_reference: Image(self.centerline_reference_filename).save(os.path.join(path_tmp, "centerline_ref.nii.gz")) if self.discs_input_filename != '': Image(self.discs_input_filename).save(os.path.join(path_tmp, "labels_input.nii.gz")) if self.discs_ref_filename != '': Image(self.discs_ref_filename).save(os.path.join(path_tmp, "labels_ref.nii.gz")) # go to tmp folder curdir = os.getcwd() os.chdir(path_tmp) # Change orientation of the input centerline into RPI image_centerline = Image("centerline.nii.gz").change_orientation("RPI").save("centerline_rpi.nii.gz", mutable=True) # Get dimension nx, ny, nz, nt, px, py, pz, pt = image_centerline.dim if self.speed_factor != 1.0: intermediate_resampling = True px_r, py_r, pz_r = px * self.speed_factor, py * self.speed_factor, pz * self.speed_factor else: intermediate_resampling = False if intermediate_resampling: mv('centerline_rpi.nii.gz', 'centerline_rpi_native.nii.gz') pz_native = pz # TODO: remove system call run_proc(['sct_resample', '-i', 'centerline_rpi_native.nii.gz', '-mm', str(px_r) + 'x' + str(py_r) + 'x' + str(pz_r), '-o', 'centerline_rpi.nii.gz']) image_centerline = Image('centerline_rpi.nii.gz') nx, ny, nz, nt, px, py, pz, pt = image_centerline.dim if np.min(image_centerline.data) < 0 or np.max(image_centerline.data) > 1: image_centerline.data[image_centerline.data < 0] = 0 image_centerline.data[image_centerline.data > 1] = 1 image_centerline.save() # 2. extract bspline fitting of the centerline, and its derivatives img_ctl = Image('centerline_rpi.nii.gz') centerline = _get_centerline(img_ctl, self.param_centerline, verbose) number_of_points = centerline.number_of_points # ========================================================================================== logger.info('Create the straight space and the safe zone') # 3. compute length of centerline # compute the length of the spinal cord based on fitted centerline and size of centerline in z direction # Computation of the safe zone. # The safe zone is defined as the length of the spinal cord for which an axial segmentation will be complete # The safe length (to remove) is computed using the safe radius (given as parameter) and the angle of the # last centerline point with the inferior-superior direction. Formula: Ls = Rs * sin(angle) # Calculate Ls for both edges and remove appropriate number of centerline points radius_safe = 0.0 # mm # inferior edge u = centerline.derivatives[0] v = np.array([0, 0, -1]) angle_inferior = np.arctan2(np.linalg.norm(np.cross(u, v)), np.dot(u, v)) length_safe_inferior = radius_safe * np.sin(angle_inferior) # superior edge u = centerline.derivatives[-1] v = np.array([0, 0, 1]) angle_superior = np.arctan2(np.linalg.norm(np.cross(u, v)), np.dot(u, v)) length_safe_superior = radius_safe * np.sin(angle_superior) # remove points inferior_bound = bisect.bisect(centerline.progressive_length, length_safe_inferior) - 1 superior_bound = centerline.number_of_points - bisect.bisect(centerline.progressive_length_inverse, length_safe_superior) z_centerline = centerline.points[:, 2] length_centerline = centerline.length size_z_centerline = z_centerline[-1] - z_centerline[0] # compute the size factor between initial centerline and straight bended centerline factor_curved_straight = length_centerline / size_z_centerline middle_slice = (z_centerline[0] + z_centerline[-1]) / 2.0 bound_curved = [z_centerline[inferior_bound], z_centerline[superior_bound]] bound_straight = [(z_centerline[inferior_bound] - middle_slice) * factor_curved_straight + middle_slice, (z_centerline[superior_bound] - middle_slice) * factor_curved_straight + middle_slice] logger.info('Length of spinal cord: {}'.format(length_centerline)) logger.info('Size of spinal cord in z direction: {}'.format(size_z_centerline)) logger.info('Ratio length/size: {}'.format(factor_curved_straight)) logger.info('Safe zone boundaries (curved space): {}'.format(bound_curved)) logger.info('Safe zone boundaries (straight space): {}'.format(bound_straight)) # 4. compute and generate straight space # points along curved centerline are already regularly spaced. # calculate position of points along straight centerline # Create straight NIFTI volumes. # ========================================================================================== # TODO: maybe this if case is not needed? if self.use_straight_reference: image_centerline_pad = Image('centerline_rpi.nii.gz') nx, ny, nz, nt, px, py, pz, pt = image_centerline_pad.dim fname_ref = 'centerline_ref_rpi.nii.gz' image_centerline_straight = Image('centerline_ref.nii.gz') \ .change_orientation("RPI") \ .save(fname_ref, mutable=True) centerline_straight = _get_centerline(image_centerline_straight, self.param_centerline, verbose) nx_s, ny_s, nz_s, nt_s, px_s, py_s, pz_s, pt_s = image_centerline_straight.dim # Prepare warping fields headers hdr_warp = image_centerline_pad.hdr.copy() hdr_warp.set_data_dtype('float32') hdr_warp_s = image_centerline_straight.hdr.copy() hdr_warp_s.set_data_dtype('float32') if self.discs_input_filename != "" and self.discs_ref_filename != "": discs_input_image = Image('labels_input.nii.gz') coord = discs_input_image.getNonZeroCoordinates(sorting='z', reverse_coord=True) coord_physical = [] for c in coord: c_p = discs_input_image.transfo_pix2phys([[c.x, c.y, c.z]]).tolist()[0] c_p.append(c.value) coord_physical.append(c_p) centerline.compute_vertebral_distribution(coord_physical) centerline.save_centerline(image=discs_input_image, fname_output='discs_input_image.nii.gz') discs_ref_image = Image('labels_ref.nii.gz') coord = discs_ref_image.getNonZeroCoordinates(sorting='z', reverse_coord=True) coord_physical = [] for c in coord: c_p = discs_ref_image.transfo_pix2phys([[c.x, c.y, c.z]]).tolist()[0] c_p.append(c.value) coord_physical.append(c_p) centerline_straight.compute_vertebral_distribution(coord_physical) centerline_straight.save_centerline(image=discs_ref_image, fname_output='discs_ref_image.nii.gz') else: logger.info('Pad input volume to account for spinal cord length...') start_point, end_point = bound_straight[0], bound_straight[1] offset_z = 0 # if the destination image is resampled, we still create the straight reference space with the native # resolution. # TODO: Maybe this if case is not needed? if intermediate_resampling: padding_z = int(np.ceil(1.5 * ((length_centerline - size_z_centerline) / 2.0) / pz_native)) run_proc( ['sct_image', '-i', 'centerline_rpi_native.nii.gz', '-o', 'tmp.centerline_pad_native.nii.gz', '-pad', '0,0,' + str(padding_z)]) image_centerline_pad = Image('centerline_rpi_native.nii.gz') nx, ny, nz, nt, px, py, pz, pt = image_centerline_pad.dim start_point_coord_native = image_centerline_pad.transfo_phys2pix([[0, 0, start_point]])[0] end_point_coord_native = image_centerline_pad.transfo_phys2pix([[0, 0, end_point]])[0] straight_size_x = int(self.xy_size / px) straight_size_y = int(self.xy_size / py) warp_space_x = [int(np.round(nx / 2)) - straight_size_x, int(np.round(nx / 2)) + straight_size_x] warp_space_y = [int(np.round(ny / 2)) - straight_size_y, int(np.round(ny / 2)) + straight_size_y] if warp_space_x[0] < 0: warp_space_x[1] += warp_space_x[0] - 2 warp_space_x[0] = 0 if warp_space_y[0] < 0: warp_space_y[1] += warp_space_y[0] - 2 warp_space_y[0] = 0 spec = dict(( (0, warp_space_x), (1, warp_space_y), (2, (0, end_point_coord_native[2] - start_point_coord_native[2])), )) spatial_crop(Image("tmp.centerline_pad_native.nii.gz"), spec).save( "tmp.centerline_pad_crop_native.nii.gz") fname_ref = 'tmp.centerline_pad_crop_native.nii.gz' offset_z = 4 else: fname_ref = 'tmp.centerline_pad_crop.nii.gz' nx, ny, nz, nt, px, py, pz, pt = image_centerline.dim padding_z = int(np.ceil(1.5 * ((length_centerline - size_z_centerline) / 2.0) / pz)) + offset_z image_centerline_pad = pad_image(image_centerline, pad_z_i=padding_z, pad_z_f=padding_z) nx, ny, nz = image_centerline_pad.data.shape hdr_warp = image_centerline_pad.hdr.copy() hdr_warp.set_data_dtype('float32') start_point_coord = image_centerline_pad.transfo_phys2pix([[0, 0, start_point]])[0] end_point_coord = image_centerline_pad.transfo_phys2pix([[0, 0, end_point]])[0] straight_size_x = int(self.xy_size / px) straight_size_y = int(self.xy_size / py) warp_space_x = [int(np.round(nx / 2)) - straight_size_x, int(np.round(nx / 2)) + straight_size_x] warp_space_y = [int(np.round(ny / 2)) - straight_size_y, int(np.round(ny / 2)) + straight_size_y] if warp_space_x[0] < 0: warp_space_x[1] += warp_space_x[0] - 2 warp_space_x[0] = 0 if warp_space_x[1] >= nx: warp_space_x[1] = nx - 1 if warp_space_y[0] < 0: warp_space_y[1] += warp_space_y[0] - 2 warp_space_y[0] = 0 if warp_space_y[1] >= ny: warp_space_y[1] = ny - 1 spec = dict(( (0, warp_space_x), (1, warp_space_y), (2, (0, end_point_coord[2] - start_point_coord[2] + offset_z)), )) image_centerline_straight = spatial_crop(image_centerline_pad, spec) nx_s, ny_s, nz_s, nt_s, px_s, py_s, pz_s, pt_s = image_centerline_straight.dim hdr_warp_s = image_centerline_straight.hdr.copy() hdr_warp_s.set_data_dtype('float32') if self.template_orientation == 1: raise NotImplementedError() start_point_coord = image_centerline_pad.transfo_phys2pix([[0, 0, start_point]])[0] end_point_coord = image_centerline_pad.transfo_phys2pix([[0, 0, end_point]])[0] number_of_voxel = nx * ny * nz logger.debug('Number of voxels: {}'.format(number_of_voxel)) time_centerlines = time.time() coord_straight = np.empty((number_of_points, 3)) coord_straight[..., 0] = int(np.round(nx_s / 2)) coord_straight[..., 1] = int(np.round(ny_s / 2)) coord_straight[..., 2] = np.linspace(0, end_point_coord[2] - start_point_coord[2], number_of_points) coord_phys_straight = image_centerline_straight.transfo_pix2phys(coord_straight) derivs_straight = np.empty((number_of_points, 3)) derivs_straight[..., 0] = derivs_straight[..., 1] = 0 derivs_straight[..., 2] = 1 dx_straight, dy_straight, dz_straight = derivs_straight.T centerline_straight = Centerline(coord_phys_straight[:, 0], coord_phys_straight[:, 1], coord_phys_straight[:, 2], dx_straight, dy_straight, dz_straight) time_centerlines = time.time() - time_centerlines logger.info('Time to generate centerline: {} ms'.format(np.round(time_centerlines * 1000.0))) if verbose == 2: # TODO: use OO import matplotlib.pyplot as plt from datetime import datetime curved_points = centerline.progressive_length straight_points = centerline_straight.progressive_length range_points = np.linspace(0, 1, number_of_points) dist_curved = np.zeros(number_of_points) dist_straight = np.zeros(number_of_points) for i in range(1, number_of_points): dist_curved[i] = dist_curved[i - 1] + curved_points[i - 1] / centerline.length dist_straight[i] = dist_straight[i - 1] + straight_points[i - 1] / centerline_straight.length plt.plot(range_points, dist_curved) plt.plot(range_points, dist_straight) plt.grid(True) plt.savefig('fig_straighten_' + datetime.now().strftime("%y%m%d%H%M%S%f") + '.png') plt.close() # alignment_mode = 'length' alignment_mode = 'levels' lookup_curved2straight = list(range(centerline.number_of_points)) if self.discs_input_filename != "": # create look-up table curved to straight for index in range(centerline.number_of_points): disc_label = centerline.l_points[index] if alignment_mode == 'length': relative_position = centerline.dist_points[index] else: relative_position = centerline.dist_points_rel[index] idx_closest = centerline_straight.get_closest_to_absolute_position(disc_label, relative_position, backup_index=index, backup_centerline=centerline_straight, mode=alignment_mode) if idx_closest is not None: lookup_curved2straight[index] = idx_closest else: lookup_curved2straight[index] = 0 for p in range(0, len(lookup_curved2straight) // 2): if lookup_curved2straight[p] == lookup_curved2straight[p + 1]: lookup_curved2straight[p] = 0 else: break for p in range(len(lookup_curved2straight) - 1, len(lookup_curved2straight) // 2, -1): if lookup_curved2straight[p] == lookup_curved2straight[p - 1]: lookup_curved2straight[p] = 0 else: break lookup_curved2straight = np.array(lookup_curved2straight) lookup_straight2curved = list(range(centerline_straight.number_of_points)) if self.discs_input_filename != "": for index in range(centerline_straight.number_of_points): disc_label = centerline_straight.l_points[index] if alignment_mode == 'length': relative_position = centerline_straight.dist_points[index] else: relative_position = centerline_straight.dist_points_rel[index] idx_closest = centerline.get_closest_to_absolute_position(disc_label, relative_position, backup_index=index, backup_centerline=centerline_straight, mode=alignment_mode) if idx_closest is not None: lookup_straight2curved[index] = idx_closest for p in range(0, len(lookup_straight2curved) // 2): if lookup_straight2curved[p] == lookup_straight2curved[p + 1]: lookup_straight2curved[p] = 0 else: break for p in range(len(lookup_straight2curved) - 1, len(lookup_straight2curved) // 2, -1): if lookup_straight2curved[p] == lookup_straight2curved[p - 1]: lookup_straight2curved[p] = 0 else: break lookup_straight2curved = np.array(lookup_straight2curved) # Create volumes containing curved and straight warping fields data_warp_curved2straight = np.zeros((nx_s, ny_s, nz_s, 1, 3)) data_warp_straight2curved = np.zeros((nx, ny, nz, 1, 3)) # 5. compute transformations # Curved and straight images and the same dimensions, so we compute both warping fields at the same time. # b. determine which plane of spinal cord centreline it is included if self.curved2straight: for u in sct_progress_bar(range(nz_s)): x_s, y_s, z_s = np.mgrid[0:nx_s, 0:ny_s, u:u + 1] indexes_straight = np.array(list(zip(x_s.ravel(), y_s.ravel(), z_s.ravel()))) physical_coordinates_straight = image_centerline_straight.transfo_pix2phys(indexes_straight) nearest_indexes_straight = centerline_straight.find_nearest_indexes(physical_coordinates_straight) distances_straight = centerline_straight.get_distances_from_planes(physical_coordinates_straight, nearest_indexes_straight) lookup = lookup_straight2curved[nearest_indexes_straight] indexes_out_distance_straight = np.logical_or( np.logical_or(distances_straight > self.threshold_distance, distances_straight < -self.threshold_distance), lookup == 0) projected_points_straight = centerline_straight.get_projected_coordinates_on_planes( physical_coordinates_straight, nearest_indexes_straight) coord_in_planes_straight = centerline_straight.get_in_plans_coordinates(projected_points_straight, nearest_indexes_straight) coord_straight2curved = centerline.get_inverse_plans_coordinates(coord_in_planes_straight, lookup) displacements_straight = coord_straight2curved - physical_coordinates_straight # Invert Z coordinate as ITK & ANTs physical coordinate system is LPS- (RAI+) # while ours is LPI- # Refs: https://sourceforge.net/p/advants/discussion/840261/thread/2a1e9307/#fb5a # https://www.slicer.org/wiki/Coordinate_systems displacements_straight[:, 2] = -displacements_straight[:, 2] displacements_straight[indexes_out_distance_straight] = [100000.0, 100000.0, 100000.0] data_warp_curved2straight[indexes_straight[:, 0], indexes_straight[:, 1], indexes_straight[:, 2], 0, :]\ = -displacements_straight if self.straight2curved: for u in sct_progress_bar(range(nz)): x, y, z = np.mgrid[0:nx, 0:ny, u:u + 1] indexes = np.array(list(zip(x.ravel(), y.ravel(), z.ravel()))) physical_coordinates = image_centerline_pad.transfo_pix2phys(indexes) nearest_indexes_curved = centerline.find_nearest_indexes(physical_coordinates) distances_curved = centerline.get_distances_from_planes(physical_coordinates, nearest_indexes_curved) lookup = lookup_curved2straight[nearest_indexes_curved] indexes_out_distance_curved = np.logical_or( np.logical_or(distances_curved > self.threshold_distance, distances_curved < -self.threshold_distance), lookup == 0) projected_points_curved = centerline.get_projected_coordinates_on_planes(physical_coordinates, nearest_indexes_curved) coord_in_planes_curved = centerline.get_in_plans_coordinates(projected_points_curved, nearest_indexes_curved) coord_curved2straight = centerline_straight.points[lookup] coord_curved2straight[:, 0:2] += coord_in_planes_curved[:, 0:2] coord_curved2straight[:, 2] += distances_curved displacements_curved = coord_curved2straight - physical_coordinates displacements_curved[:, 2] = -displacements_curved[:, 2] displacements_curved[indexes_out_distance_curved] = [100000.0, 100000.0, 100000.0] data_warp_straight2curved[indexes[:, 0], indexes[:, 1], indexes[:, 2], 0, :] = -displacements_curved # Creation of the safe zone based on pre-calculated safe boundaries coord_bound_curved_inf, coord_bound_curved_sup = image_centerline_pad.transfo_phys2pix( [[0, 0, bound_curved[0]]]), image_centerline_pad.transfo_phys2pix([[0, 0, bound_curved[1]]]) coord_bound_straight_inf, coord_bound_straight_sup = image_centerline_straight.transfo_phys2pix( [[0, 0, bound_straight[0]]]), image_centerline_straight.transfo_phys2pix([[0, 0, bound_straight[1]]]) if radius_safe > 0: data_warp_curved2straight[:, :, 0:coord_bound_straight_inf[0][2], 0, :] = 100000.0 data_warp_curved2straight[:, :, coord_bound_straight_sup[0][2]:, 0, :] = 100000.0 data_warp_straight2curved[:, :, 0:coord_bound_curved_inf[0][2], 0, :] = 100000.0 data_warp_straight2curved[:, :, coord_bound_curved_sup[0][2]:, 0, :] = 100000.0 # Generate warp files as a warping fields hdr_warp_s.set_intent('vector', (), '') hdr_warp_s.set_data_dtype('float32') hdr_warp.set_intent('vector', (), '') hdr_warp.set_data_dtype('float32') if self.curved2straight: img = Nifti1Image(data_warp_curved2straight, None, hdr_warp_s) save(img, 'tmp.curve2straight.nii.gz') logger.info('Warping field generated: tmp.curve2straight.nii.gz') if self.straight2curved: img = Nifti1Image(data_warp_straight2curved, None, hdr_warp) save(img, 'tmp.straight2curve.nii.gz') logger.info('Warping field generated: tmp.straight2curve.nii.gz') image_centerline_straight.save(fname_ref) if self.curved2straight: logger.info('Apply transformation to input image...') run_proc(['isct_antsApplyTransforms', '-d', '3', '-r', fname_ref, '-i', 'data.nii', '-o', 'tmp.anat_rigid_warp.nii.gz', '-t', 'tmp.curve2straight.nii.gz', '-n', 'BSpline[3]'], is_sct_binary=True, verbose=verbose) if self.accuracy_results: time_accuracy_results = time.time() # compute the error between the straightened centerline/segmentation and the central vertical line. # Ideally, the error should be zero. # Apply deformation to input image logger.info('Apply transformation to centerline image...') run_proc(['isct_antsApplyTransforms', '-d', '3', '-r', fname_ref, '-i', 'centerline.nii.gz', '-o', 'tmp.centerline_straight.nii.gz', '-t', 'tmp.curve2straight.nii.gz', '-n', 'NearestNeighbor'], is_sct_binary=True, verbose=verbose) file_centerline_straight = Image('tmp.centerline_straight.nii.gz', verbose=verbose) nx, ny, nz, nt, px, py, pz, pt = file_centerline_straight.dim coordinates_centerline = file_centerline_straight.getNonZeroCoordinates(sorting='z') mean_coord = [] for z in range(coordinates_centerline[0].z, coordinates_centerline[-1].z): temp_mean = [coord.value for coord in coordinates_centerline if coord.z == z] if temp_mean: mean_value = np.mean(temp_mean) mean_coord.append( np.mean([[coord.x * coord.value / mean_value, coord.y * coord.value / mean_value] for coord in coordinates_centerline if coord.z == z], axis=0)) # compute error between the straightened centerline and the straight line. x0 = file_centerline_straight.data.shape[0] / 2.0 y0 = file_centerline_straight.data.shape[1] / 2.0 count_mean = 0 if number_of_points >= 10: mean_c = mean_coord[2:-2] # we don't include the four extrema because there are usually messy. else: mean_c = mean_coord for coord_z in mean_c: if not np.isnan(np.sum(coord_z)): dist = ((x0 - coord_z[0]) * px) ** 2 + ((y0 - coord_z[1]) * py) ** 2 self.mse_straightening += dist dist = np.sqrt(dist) if dist > self.max_distance_straightening: self.max_distance_straightening = dist count_mean += 1 self.mse_straightening = np.sqrt(self.mse_straightening / float(count_mean)) self.elapsed_time_accuracy = time.time() - time_accuracy_results os.chdir(curdir) # Generate output file (in current folder) # TODO: do not uncompress the warping field, it is too time consuming! logger.info('Generate output files...') if self.curved2straight: generate_output_file(os.path.join(path_tmp, "tmp.curve2straight.nii.gz"), os.path.join(self.path_output, "warp_curve2straight.nii.gz"), verbose) if self.straight2curved: generate_output_file(os.path.join(path_tmp, "tmp.straight2curve.nii.gz"), os.path.join(self.path_output, "warp_straight2curve.nii.gz"), verbose) # create ref_straight.nii.gz file that can be used by other SCT functions that need a straight reference space if self.curved2straight: copy(os.path.join(path_tmp, "tmp.anat_rigid_warp.nii.gz"), os.path.join(self.path_output, "straight_ref.nii.gz")) # move straightened input file if fname_output == '': fname_straight = generate_output_file(os.path.join(path_tmp, "tmp.anat_rigid_warp.nii.gz"), os.path.join(self.path_output, file_anat + "_straight" + ext_anat), verbose) else: fname_straight = generate_output_file(os.path.join(path_tmp, "tmp.anat_rigid_warp.nii.gz"), os.path.join(self.path_output, fname_output), verbose) # straightened anatomic # Remove temporary files if remove_temp_files: logger.info('Remove temporary files...') rmtree(path_tmp) if self.accuracy_results: logger.info('Maximum x-y error: {} mm'.format(self.max_distance_straightening)) logger.info('Accuracy of straightening (MSE): {} mm'.format(self.mse_straightening)) # display elapsed time self.elapsed_time = int(np.round(time.time() - start_time)) return fname_straight
def main(argv=None): """Main function.""" parser = get_parser() arguments = parser.parse_args(argv if argv else ['--help']) verbose = arguments.v set_global_loglevel(verbose=verbose) fname_image = os.path.abspath(arguments.i) contrast_type = arguments.c ctr_algo = arguments.centerline if arguments.brain is None: if contrast_type in ['t2s', 'dwi']: brain_bool = False if contrast_type in ['t1', 't2']: brain_bool = True else: brain_bool = bool(arguments.brain) if bool(arguments.brain) and ctr_algo == 'svm': printv('Please only use the flag "-brain 1" with "-centerline cnn".', 1, 'warning') sys.exit(1) kernel_size = arguments.kernel if kernel_size == '3d' and contrast_type == 'dwi': kernel_size = '2d' printv( '3D kernel model for dwi contrast is not available. 2D kernel model is used instead.', type="warning") if ctr_algo == 'file' and arguments.file_centerline is None: printv( 'Please use the flag -file_centerline to indicate the centerline filename.', 1, 'warning') sys.exit(1) if arguments.file_centerline is not None: manual_centerline_fname = arguments.file_centerline ctr_algo = 'file' else: manual_centerline_fname = None threshold = arguments.thr if threshold is not None: if threshold > 1.0 or (threshold < 0.0 and threshold != -1.0): raise SyntaxError( "Threshold should be between 0 and 1, or equal to -1 (no threshold)" ) remove_temp_files = arguments.r path_qc = arguments.qc qc_dataset = arguments.qc_dataset qc_subject = arguments.qc_subject output_folder = arguments.ofolder # check if input image is 2D or 3D check_dim(fname_image, dim_lst=[2, 3]) # Segment image im_image = Image(fname_image) # note: below we pass im_image.copy() otherwise the field absolutepath becomes None after execution of this function im_seg, im_image_RPI_upsamp, im_seg_RPI_upsamp = \ deep_segmentation_spinalcord(im_image.copy(), contrast_type, ctr_algo=ctr_algo, ctr_file=manual_centerline_fname, brain_bool=brain_bool, kernel_size=kernel_size, threshold_seg=threshold, remove_temp_files=remove_temp_files, verbose=verbose) # Save segmentation fname_seg = os.path.abspath( os.path.join( output_folder, extract_fname(fname_image)[1] + '_seg' + extract_fname(fname_image)[2])) im_seg.save(fname_seg) # Generate QC report if path_qc is not None: generate_qc(fname_image, fname_seg=fname_seg, args=sys.argv[1:], path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject, process='sct_deepseg_sc') display_viewer_syntax([fname_image, fname_seg], colormaps=['gray', 'red'], opacities=['', '0.7'])
def reorient_data(self): for f in self.fname_metric_lst: os.rename(self.fname_metric_lst[f], add_suffix("".join(extract_fname(self.param.fname_im)[1:]), '_2reorient')) im = Image(add_suffix("".join(extract_fname(self.param.fname_im)[1:]), '_2reorient')) \ .change_orientation(self.orientation_im) \ .save(self.fname_metric_lst[f])
def main(argv=None): parser = get_parser() arguments = parser.parse_args(argv) verbose = arguments.v set_global_loglevel(verbose=verbose) # initializations initz = '' initcenter = '' fname_initlabel = '' file_labelz = 'labelz.nii.gz' param = Param() fname_in = os.path.abspath(arguments.i) fname_seg = os.path.abspath(arguments.s) contrast = arguments.c path_template = os.path.abspath(arguments.t) scale_dist = arguments.scale_dist path_output = arguments.ofolder param.path_qc = arguments.qc if arguments.discfile is not None: fname_disc = os.path.abspath(arguments.discfile) else: fname_disc = None if arguments.initz is not None: initz = arguments.initz if len(initz) != 2: raise ValueError('--initz takes two arguments: position in superior-inferior direction, label value') if arguments.initcenter is not None: initcenter = arguments.initcenter # if user provided text file, parse and overwrite arguments if arguments.initfile is not None: file = open(arguments.initfile, 'r') initfile = ' ' + file.read().replace('\n', '') arg_initfile = initfile.split(' ') for idx_arg, arg in enumerate(arg_initfile): if arg == '-initz': initz = [int(x) for x in arg_initfile[idx_arg + 1].split(',')] if len(initz) != 2: raise ValueError('--initz takes two arguments: position in superior-inferior direction, label value') if arg == '-initcenter': initcenter = int(arg_initfile[idx_arg + 1]) if arguments.initlabel is not None: # get absolute path of label fname_initlabel = os.path.abspath(arguments.initlabel) if arguments.param is not None: param.update(arguments.param[0]) remove_temp_files = arguments.r clean_labels = arguments.clean_labels laplacian = arguments.laplacian path_tmp = tmp_create(basename="label_vertebrae") # Copying input data to tmp folder printv('\nCopying input data to tmp folder...', verbose) Image(fname_in).save(os.path.join(path_tmp, "data.nii")) Image(fname_seg).save(os.path.join(path_tmp, "segmentation.nii")) # Go go temp folder curdir = os.getcwd() os.chdir(path_tmp) # Straighten spinal cord printv('\nStraighten spinal cord...', verbose) # check if warp_curve2straight and warp_straight2curve already exist (i.e. no need to do it another time) cache_sig = cache_signature( input_files=[fname_in, fname_seg], ) cachefile = os.path.join(curdir, "straightening.cache") if cache_valid(cachefile, cache_sig) and os.path.isfile(os.path.join(curdir, "warp_curve2straight.nii.gz")) and os.path.isfile(os.path.join(curdir, "warp_straight2curve.nii.gz")) and os.path.isfile(os.path.join(curdir, "straight_ref.nii.gz")): # if they exist, copy them into current folder printv('Reusing existing warping field which seems to be valid', verbose, 'warning') copy(os.path.join(curdir, "warp_curve2straight.nii.gz"), 'warp_curve2straight.nii.gz') copy(os.path.join(curdir, "warp_straight2curve.nii.gz"), 'warp_straight2curve.nii.gz') copy(os.path.join(curdir, "straight_ref.nii.gz"), 'straight_ref.nii.gz') # apply straightening s, o = run_proc(['sct_apply_transfo', '-i', 'data.nii', '-w', 'warp_curve2straight.nii.gz', '-d', 'straight_ref.nii.gz', '-o', 'data_straight.nii']) else: sct_straighten_spinalcord.main(argv=[ '-i', 'data.nii', '-s', 'segmentation.nii', '-r', str(remove_temp_files), '-v', str(verbose), ]) cache_save(cachefile, cache_sig) # resample to 0.5mm isotropic to match template resolution printv('\nResample to 0.5mm isotropic...', verbose) s, o = run_proc(['sct_resample', '-i', 'data_straight.nii', '-mm', '0.5x0.5x0.5', '-x', 'linear', '-o', 'data_straightr.nii'], verbose=verbose) # Apply straightening to segmentation # N.B. Output is RPI printv('\nApply straightening to segmentation...', verbose) run_proc('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' % ('segmentation.nii', 'data_straightr.nii', 'warp_curve2straight.nii.gz', 'segmentation_straight.nii', 'Linear'), verbose=verbose, is_sct_binary=True, ) # Threshold segmentation at 0.5 img = Image('segmentation_straight.nii') img.data = threshold(img.data, 0.5) img.save() # If disc label file is provided, label vertebrae using that file instead of automatically if fname_disc: # Apply straightening to disc-label printv('\nApply straightening to disc labels...', verbose) run_proc('sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' % (fname_disc, 'data_straightr.nii', 'warp_curve2straight.nii.gz', 'labeldisc_straight.nii.gz', 'label'), verbose=verbose ) label_vert('segmentation_straight.nii', 'labeldisc_straight.nii.gz', verbose=1) else: # create label to identify disc printv('\nCreate label to identify disc...', verbose) fname_labelz = os.path.join(path_tmp, file_labelz) if initz or initcenter: if initcenter: # find z centered in FOV nii = Image('segmentation.nii').change_orientation("RPI") nx, ny, nz, nt, px, py, pz, pt = nii.dim # Get dimensions z_center = int(np.round(nz / 2)) # get z_center initz = [z_center, initcenter] im_label = create_labels_along_segmentation(Image('segmentation.nii'), [(initz[0], initz[1])]) im_label.data = dilate(im_label.data, 3, 'ball') im_label.save(fname_labelz) elif fname_initlabel: Image(fname_initlabel).save(fname_labelz) else: # automatically finds C2-C3 disc im_data = Image('data.nii') im_seg = Image('segmentation.nii') if not remove_temp_files: # because verbose is here also used for keeping temp files verbose_detect_c2c3 = 2 else: verbose_detect_c2c3 = 0 im_label_c2c3 = detect_c2c3(im_data, im_seg, contrast, verbose=verbose_detect_c2c3) ind_label = np.where(im_label_c2c3.data) if not np.size(ind_label) == 0: im_label_c2c3.data[ind_label] = 3 else: printv('Automatic C2-C3 detection failed. Please provide manual label with sct_label_utils', 1, 'error') sys.exit() im_label_c2c3.save(fname_labelz) # dilate label so it is not lost when applying warping dilate(Image(fname_labelz), 3, 'ball').save(fname_labelz) # Apply straightening to z-label printv('\nAnd apply straightening to label...', verbose) run_proc('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' % (file_labelz, 'data_straightr.nii', 'warp_curve2straight.nii.gz', 'labelz_straight.nii.gz', 'NearestNeighbor'), verbose=verbose, is_sct_binary=True, ) # get z value and disk value to initialize labeling printv('\nGet z and disc values from straight label...', verbose) init_disc = get_z_and_disc_values_from_label('labelz_straight.nii.gz') printv('.. ' + str(init_disc), verbose) # apply laplacian filtering if laplacian: printv('\nApply Laplacian filter...', verbose) img = Image("data_straightr.nii") # apply std dev to each axis of the image sigmas = [1 for i in range(len(img.data.shape))] # adjust sigma based on voxel size sigmas = [sigmas[i] / img.dim[i + 4] for i in range(3)] # smooth data img.data = laplacian(img.data, sigmas) img.save() # detect vertebral levels on straight spinal cord init_disc[1] = init_disc[1] - 1 vertebral_detection('data_straightr.nii', 'segmentation_straight.nii', contrast, param, init_disc=init_disc, verbose=verbose, path_template=path_template, path_output=path_output, scale_dist=scale_dist) # un-straighten labeled spinal cord printv('\nUn-straighten labeling...', verbose) run_proc('isct_antsApplyTransforms -d 3 -i %s -r %s -t %s -o %s -n %s' % ('segmentation_straight_labeled.nii', 'segmentation.nii', 'warp_straight2curve.nii.gz', 'segmentation_labeled.nii', 'NearestNeighbor'), verbose=verbose, is_sct_binary=True, ) if clean_labels: # Clean labeled segmentation printv('\nClean labeled segmentation (correct interpolation errors)...', verbose) clean_labeled_segmentation('segmentation_labeled.nii', 'segmentation.nii', 'segmentation_labeled.nii') # label discs printv('\nLabel discs...', verbose) printv('\nUn-straighten labeled discs...', verbose) run_proc('sct_apply_transfo -i %s -d %s -w %s -o %s -x %s' % ('segmentation_straight_labeled_disc.nii', 'segmentation.nii', 'warp_straight2curve.nii.gz', 'segmentation_labeled_disc.nii', 'label'), verbose=verbose, is_sct_binary=True, ) # come back os.chdir(curdir) # Generate output files path_seg, file_seg, ext_seg = extract_fname(fname_seg) fname_seg_labeled = os.path.join(path_output, file_seg + '_labeled' + ext_seg) printv('\nGenerate output files...', verbose) generate_output_file(os.path.join(path_tmp, "segmentation_labeled.nii"), fname_seg_labeled) generate_output_file(os.path.join(path_tmp, "segmentation_labeled_disc.nii"), os.path.join(path_output, file_seg + '_labeled_discs' + ext_seg)) # copy straightening files in case subsequent SCT functions need them generate_output_file(os.path.join(path_tmp, "warp_curve2straight.nii.gz"), os.path.join(path_output, "warp_curve2straight.nii.gz"), verbose=verbose) generate_output_file(os.path.join(path_tmp, "warp_straight2curve.nii.gz"), os.path.join(path_output, "warp_straight2curve.nii.gz"), verbose=verbose) generate_output_file(os.path.join(path_tmp, "straight_ref.nii.gz"), os.path.join(path_output, "straight_ref.nii.gz"), verbose=verbose) # Remove temporary files if remove_temp_files == 1: printv('\nRemove temporary files...', verbose) rmtree(path_tmp) # Generate QC report if param.path_qc is not None: path_qc = os.path.abspath(arguments.qc) qc_dataset = arguments.qc_dataset qc_subject = arguments.qc_subject labeled_seg_file = os.path.join(path_output, file_seg + '_labeled' + ext_seg) generate_qc(fname_in, fname_seg=labeled_seg_file, args=argv, path_qc=os.path.abspath(path_qc), dataset=qc_dataset, subject=qc_subject, process='sct_label_vertebrae') display_viewer_syntax([fname_in, fname_seg_labeled], colormaps=['', 'subcortical'], opacities=['1', '0.5'])
def propseg(img_input, options_dict): """ :param img_input: source image, to be segmented :param options_dict: arguments as dictionary :return: segmented Image """ arguments = options_dict fname_input_data = img_input.absolutepath fname_data = os.path.abspath(fname_input_data) contrast_type = arguments.c contrast_type_conversion = { 't1': 't1', 't2': 't2', 't2s': 't2', 'dwi': 't1' } contrast_type_propseg = contrast_type_conversion[contrast_type] # Starting building the command cmd = ['isct_propseg', '-t', contrast_type_propseg] if arguments.ofolder is not None: folder_output = arguments.ofolder else: folder_output = './' cmd += ['-o', folder_output] if not os.path.isdir(folder_output) and os.path.exists(folder_output): logger.error("output directory %s is not a valid directory" % folder_output) if not os.path.exists(folder_output): os.makedirs(folder_output) if arguments.down is not None: cmd += ["-down", str(arguments.down)] if arguments.up is not None: cmd += ["-up", str(arguments.up)] remove_temp_files = arguments.r verbose = int(arguments.v) init_sct(log_level=verbose, update=True) # Update log level # Update for propseg binary if verbose > 0: cmd += ["-verbose"] # Output options if arguments.mesh is not None: cmd += ["-mesh"] if arguments.centerline_binary is not None: cmd += ["-centerline-binary"] if arguments.CSF is not None: cmd += ["-CSF"] if arguments.centerline_coord is not None: cmd += ["-centerline-coord"] if arguments.cross is not None: cmd += ["-cross"] if arguments.init_tube is not None: cmd += ["-init-tube"] if arguments.low_resolution_mesh is not None: cmd += ["-low-resolution-mesh"] # TODO: Not present. Why is this here? Was this renamed? # if arguments.detect_nii is not None: # cmd += ["-detect-nii"] # TODO: Not present. Why is this here? Was this renamed? # if arguments.detect_png is not None: # cmd += ["-detect-png"] # Helping options use_viewer = None use_optic = True # enabled by default init_option = None rescale_header = arguments.rescale if arguments.init is not None: init_option = float(arguments.init) if init_option < 0: printv( 'Command-line usage error: ' + str(init_option) + " is not a valid value for '-init'", 1, 'error') sys.exit(1) if arguments.init_centerline is not None: if str(arguments.init_centerline) == "viewer": use_viewer = "centerline" elif str(arguments.init_centerline) == "hough": use_optic = False else: if rescale_header is not 1: fname_labels_viewer = func_rescale_header(str( arguments.init_centerline), rescale_header, verbose=verbose) else: fname_labels_viewer = str(arguments.init_centerline) cmd += ["-init-centerline", fname_labels_viewer] use_optic = False if arguments.init_mask is not None: if str(arguments.init_mask) == "viewer": use_viewer = "mask" else: if rescale_header is not 1: fname_labels_viewer = func_rescale_header( str(arguments.init_mask), rescale_header) else: fname_labels_viewer = str(arguments.init_mask) cmd += ["-init-mask", fname_labels_viewer] use_optic = False if arguments.mask_correction is not None: cmd += ["-mask-correction", str(arguments.mask_correction)] if arguments.radius is not None: cmd += ["-radius", str(arguments.radius)] # TODO: Not present. Why is this here? Was this renamed? # if arguments.detect_n is not None: # cmd += ["-detect-n", str(arguments.detect_n)] # TODO: Not present. Why is this here? Was this renamed? # if arguments.detect_gap is not None: # cmd += ["-detect-gap", str(arguments.detect_gap)] # TODO: Not present. Why is this here? Was this renamed? # if arguments.init_validation is not None: # cmd += ["-init-validation"] if arguments.nbiter is not None: cmd += ["-nbiter", str(arguments.nbiter)] if arguments.max_area is not None: cmd += ["-max-area", str(arguments.max_area)] if arguments.max_deformation is not None: cmd += ["-max-deformation", str(arguments.max_deformation)] if arguments.min_contrast is not None: cmd += ["-min-contrast", str(arguments.min_contrast)] if arguments.d is not None: cmd += ["-d", str(arguments["-d"])] if arguments.distance_search is not None: cmd += ["-dsearch", str(arguments.distance_search)] if arguments.alpha is not None: cmd += ["-alpha", str(arguments.alpha)] # check if input image is in 3D. Otherwise itk image reader will cut the 4D image in 3D volumes and only take the first one. image_input = Image(fname_data) image_input_rpi = image_input.copy().change_orientation('RPI') nx, ny, nz, nt, px, py, pz, pt = image_input_rpi.dim if nt > 1: printv( 'ERROR: your input image needs to be 3D in order to be segmented.', 1, 'error') path_data, file_data, ext_data = extract_fname(fname_data) path_tmp = tmp_create(basename="label_vertebrae") # rescale header (see issue #1406) if rescale_header is not 1: fname_data_propseg = func_rescale_header(fname_data, rescale_header) else: fname_data_propseg = fname_data # add to command cmd += ['-i', fname_data_propseg] # if centerline or mask is asked using viewer if use_viewer: from spinalcordtoolbox.gui.base import AnatomicalParams from spinalcordtoolbox.gui.centerline import launch_centerline_dialog params = AnatomicalParams() if use_viewer == 'mask': params.num_points = 3 params.interval_in_mm = 15 # superior-inferior interval between two consecutive labels params.starting_slice = 'midfovminusinterval' if use_viewer == 'centerline': # setting maximum number of points to a reasonable value params.num_points = 20 params.interval_in_mm = 30 params.starting_slice = 'top' im_data = Image(fname_data_propseg) im_mask_viewer = zeros_like(im_data) # im_mask_viewer.absolutepath = add_suffix(fname_data_propseg, '_labels_viewer') controller = launch_centerline_dialog(im_data, im_mask_viewer, params) fname_labels_viewer = add_suffix(fname_data_propseg, '_labels_viewer') if not controller.saved: printv( 'The viewer has been closed before entering all manual points. Please try again.', 1, 'error') sys.exit(1) # save labels controller.as_niftii(fname_labels_viewer) # add mask filename to parameters string if use_viewer == "centerline": cmd += ["-init-centerline", fname_labels_viewer] elif use_viewer == "mask": cmd += ["-init-mask", fname_labels_viewer] # If using OptiC elif use_optic: image_centerline = optic.detect_centerline(image_input, contrast_type, verbose) fname_centerline_optic = os.path.join(path_tmp, 'centerline_optic.nii.gz') image_centerline.save(fname_centerline_optic) cmd += ["-init-centerline", fname_centerline_optic] if init_option is not None: if init_option > 1: init_option /= (nz - 1) cmd += ['-init', str(init_option)] # enabling centerline extraction by default (needed by check_and_correct_segmentation() ) cmd += ['-centerline-binary'] # run propseg status, output = run_proc(cmd, verbose, raise_exception=False, is_sct_binary=True) # check status is not 0 if not status == 0: printv( 'Automatic cord detection failed. Please initialize using -init-centerline or -init-mask (see help)', 1, 'error') sys.exit(1) # build output filename fname_seg = os.path.join(folder_output, os.path.basename(add_suffix(fname_data, "_seg"))) fname_centerline = os.path.join( folder_output, os.path.basename(add_suffix(fname_data, "_centerline"))) # in case header was rescaled, we need to update the output file names by removing the "_rescaled" if rescale_header is not 1: mv( os.path.join( folder_output, add_suffix(os.path.basename(fname_data_propseg), "_seg")), fname_seg) mv( os.path.join( folder_output, add_suffix(os.path.basename(fname_data_propseg), "_centerline")), fname_centerline) # if user was used, copy the labelled points to the output folder (they will then be scaled back) if use_viewer: fname_labels_viewer_new = os.path.join( folder_output, os.path.basename(add_suffix(fname_data, "_labels_viewer"))) copy(fname_labels_viewer, fname_labels_viewer_new) # update variable (used later) fname_labels_viewer = fname_labels_viewer_new # check consistency of segmentation if arguments.correct_seg: check_and_correct_segmentation(fname_seg, fname_centerline, folder_output=folder_output, threshold_distance=3.0, remove_temp_files=remove_temp_files, verbose=verbose) # copy header from input to segmentation to make sure qform is the same printv("Copy header input --> output(s) to make sure qform is the same.", verbose) list_fname = [fname_seg, fname_centerline] if use_viewer: list_fname.append(fname_labels_viewer) for fname in list_fname: im = Image(fname) im.header = image_input.header im.save(dtype='int8' ) # they are all binary masks hence fine to save as int8 return Image(fname_seg)
def resample_image(fname, suffix='_resampled.nii.gz', binary=False, npx=0.3, npy=0.3, thr=0.0, interpolation='spline'): """ Resampling function: add a padding, resample, crop the padding :param fname: name of the image file to be resampled :param suffix: suffix added to the original fname after resampling :param binary: boolean, image is binary or not :param npx: new pixel size in the x direction :param npy: new pixel size in the y direction :param thr: if the image is binary, it will be thresholded at thr (default=0) after the resampling :param interpolation: type of interpolation used for the resampling :return: file name after resampling (or original fname if it was already in the correct resolution) """ im_in = Image(fname) orientation = im_in.orientation if orientation != 'RPI': fname = im_in.change_orientation( im_in, 'RPI', generate_path=True).save().absolutepath nx, ny, nz, nt, px, py, pz, pt = im_in.dim if np.round(px, 2) != np.round(npx, 2) or np.round(py, 2) != np.round( npy, 2): name_resample = extract_fname(fname)[1] + suffix if binary: interpolation = 'nn' if nz == 1: # when data is 2d: we convert it to a 3d image in order to avoid conversion problem with 2d data # TODO: check if this above problem is still present (now that we are using nibabel instead of nipy) run_proc([ 'sct_image', '-i', ','.join([fname, fname]), '-concat', 'z', '-o', fname ]) run_proc([ 'sct_resample', '-i', fname, '-mm', str(npx) + 'x' + str(npy) + 'x' + str(pz), '-o', name_resample, '-x', interpolation ]) if nz == 1: # when input data was 2d: re-convert data 3d-->2d run_proc(['sct_image', '-i', name_resample, '-split', 'z']) im_split = Image( name_resample.split('.nii.gz')[0] + '_Z0000.nii.gz') im_split.save(name_resample) if binary: run_proc([ 'sct_maths', '-i', name_resample, '-bin', str(thr), '-o', name_resample ]) if orientation != 'RPI': name_resample = Image(name_resample) \ .change_orientation(orientation, generate_path=True) \ .save() \ .absolutepath return name_resample else: if orientation != 'RPI': fname = add_suffix(fname, "_RPI") im_in = change_orientation(im_in, orientation).save(fname) printv('Image resolution already ' + str(npx) + 'x' + str(npy) + 'xpz') return fname
def moco(param): """ Main function that performs motion correction. :param param: :return: """ # retrieve parameters file_data = param.file_data file_target = param.file_target folder_mat = param.mat_moco # output folder of mat file todo = param.todo suffix = param.suffix verbose = param.verbose # other parameters file_mask = 'mask.nii' printv('\nInput parameters:', param.verbose) printv(' Input file ............ ' + file_data, param.verbose) printv(' Reference file ........ ' + file_target, param.verbose) printv(' Polynomial degree ..... ' + param.poly, param.verbose) printv(' Smoothing kernel ...... ' + param.smooth, param.verbose) printv(' Gradient step ......... ' + param.gradStep, param.verbose) printv(' Metric ................ ' + param.metric, param.verbose) printv(' Sampling .............. ' + param.sampling, param.verbose) printv(' Todo .................. ' + todo, param.verbose) printv(' Mask ................. ' + param.fname_mask, param.verbose) printv(' Output mat folder ..... ' + folder_mat, param.verbose) try: os.makedirs(folder_mat) except FileExistsError: pass # Get size of data printv('\nData dimensions:', verbose) im_data = Image(param.file_data) nx, ny, nz, nt, px, py, pz, pt = im_data.dim printv( (' ' + str(nx) + ' x ' + str(ny) + ' x ' + str(nz) + ' x ' + str(nt)), verbose) # copy file_target to a temporary file printv('\nCopy file_target to a temporary file...', verbose) im_target = convert(Image(param.file_target)) im_target.save("target.nii.gz", mutable=True, verbose=0) if not param.fname_mask == '': file_target, _ = apply_mask_if_soft(file_target, param.fname_mask) # If scan is sagittal, split src and target along Z (slice) if param.is_sagittal: dim_sag = 2 # TODO: find it # z-split data (time series) im_z_list = split_data(im_data, dim=dim_sag, squeeze_data=False) file_data_splitZ = [] for im_z in im_z_list: im_z.save(verbose=0) file_data_splitZ.append(im_z.absolutepath) # z-split target im_targetz_list = split_data(Image(file_target), dim=dim_sag, squeeze_data=False) file_target_splitZ = [] for im_targetz in im_targetz_list: im_targetz.save(verbose=0) file_target_splitZ.append(im_targetz.absolutepath) # z-split mask (if exists) if not param.fname_mask == '': im_maskz_list = split_data(Image(file_mask), dim=dim_sag, squeeze_data=False) file_mask_splitZ = [] for im_maskz in im_maskz_list: im_maskz.save(verbose=0) file_mask_splitZ.append(im_maskz.absolutepath) # initialize file list for output matrices file_mat = np.empty((nz, nt), dtype=object) # axial orientation else: file_data_splitZ = [file_data] # TODO: make it absolute like above file_target_splitZ = [file_target] # TODO: make it absolute like above # initialize file list for output matrices file_mat = np.empty((1, nt), dtype=object) # deal with mask if not param.fname_mask == '': im_mask = convert(Image(param.fname_mask), squeeze_data=False) im_mask.save(file_mask, mutable=True, verbose=0) im_maskz_list = [Image(file_mask) ] # use a list with single element # Loop across file list, where each file is either a 2D volume (if sagittal) or a 3D volume (otherwise) # file_mat = tuple([[[] for i in range(nt)] for i in range(nz)]) file_data_splitZ_moco = [] printv( '\nRegister. Loop across Z (note: there is only one Z if orientation is axial)' ) for file in file_data_splitZ: iz = file_data_splitZ.index(file) # Split data along T dimension # printv('\nSplit data along T dimension.', verbose) im_z = Image(file) list_im_zt = split_data(im_z, dim=3) file_data_splitZ_splitT = [] for im_zt in list_im_zt: im_zt.save(verbose=0) file_data_splitZ_splitT.append(im_zt.absolutepath) # file_data_splitT = file_data + '_T' # Motion correction: initialization index = np.arange(nt) file_data_splitT_num = [] file_data_splitZ_splitT_moco = [] failed_transfo = [0 for i in range(nt)] # Motion correction: Loop across T for indice_index in sct_progress_bar(range(nt), unit='iter', unit_scale=False, desc="Z=" + str(iz) + "/" + str(len(file_data_splitZ) - 1), ascii=False, ncols=80): # create indices and display stuff it = index[indice_index] file_mat[iz][it] = os.path.join( folder_mat, "mat.Z") + str(iz).zfill(4) + 'T' + str(it).zfill(4) file_data_splitZ_splitT_moco.append( add_suffix(file_data_splitZ_splitT[it], '_moco')) # deal with masking (except in the 'apply' case, where masking is irrelevant) input_mask = None if not param.fname_mask == '' and not param.todo == 'apply': file_data_splitZ_splitT[it], input_mask = apply_mask_if_soft( file_data_splitZ_splitT[it], im_maskz_list[iz]) # run 3D registration failed_transfo[it] = register(param, file_data_splitZ_splitT[it], file_target_splitZ[iz], file_mat[iz][it], file_data_splitZ_splitT_moco[it], im_mask=input_mask) # average registered volume with target image # N.B. use weighted averaging: (target * nb_it + moco) / (nb_it + 1) if param.iterAvg and indice_index < 10 and failed_transfo[ it] == 0 and not param.todo == 'apply': im_targetz = Image(file_target_splitZ[iz]) data_targetz = im_targetz.data data_mocoz = Image(file_data_splitZ_splitT_moco[it]).data data_targetz = (data_targetz * (indice_index + 1) + data_mocoz) / (indice_index + 2) im_targetz.data = data_targetz im_targetz.save(verbose=0) # Replace failed transformation with the closest good one fT = [i for i, j in enumerate(failed_transfo) if j == 1] gT = [i for i, j in enumerate(failed_transfo) if j == 0] for it in range(len(fT)): abs_dist = [np.abs(gT[i] - fT[it]) for i in range(len(gT))] if not abs_dist == []: index_good = abs_dist.index(min(abs_dist)) printv( ' transfo #' + str(fT[it]) + ' --> use transfo #' + str(gT[index_good]), verbose) # copy transformation copy(file_mat[iz][gT[index_good]] + 'Warp.nii.gz', file_mat[iz][fT[it]] + 'Warp.nii.gz') # apply transformation sct_apply_transfo.main(argv=[ '-i', file_data_splitZ_splitT[fT[it]], '-d', file_target, '-w', file_mat[iz][fT[it]] + 'Warp.nii.gz', '-o', file_data_splitZ_splitT_moco[fT[it]], '-x', param.interp ]) else: # exit program if no transformation exists. printv( '\nERROR in ' + os.path.basename(__file__) + ': No good transformation exist. Exit program.\n', verbose, 'error') sys.exit(2) # Merge data along T file_data_splitZ_moco.append(add_suffix(file, suffix)) if todo != 'estimate': im_data_splitZ_splitT_moco = [ Image(fname) for fname in file_data_splitZ_splitT_moco ] im_out = concat_data(im_data_splitZ_splitT_moco, 3) im_out.absolutepath = file_data_splitZ_moco[iz] im_out.save(verbose=0) # If sagittal, merge along Z if param.is_sagittal: # TODO: im_out.dim is incorrect: Z value is one im_data_splitZ_moco = [Image(fname) for fname in file_data_splitZ_moco] im_out = concat_data(im_data_splitZ_moco, 2) dirname, basename, ext = extract_fname(file_data) path_out = os.path.join(dirname, basename + suffix + ext) im_out.absolutepath = path_out im_out.save(verbose=0) return file_mat, im_out
def moco_wrapper(param): """ Wrapper that performs motion correction. :param param: ParamMoco class :return: fname_moco """ file_data = 'data.nii' # corresponds to the full input data (e.g. dmri or fmri) file_data_dirname, file_data_basename, file_data_ext = extract_fname( file_data) file_b0 = 'b0.nii' file_datasub = 'datasub.nii' # corresponds to the full input data minus the b=0 scans (if param.is_diffusion=True) file_datasubgroup = 'datasub-groups.nii' # concatenation of the average of each file_datasub file_mask = 'mask.nii' file_moco_params_csv = 'moco_params.tsv' file_moco_params_x = 'moco_params_x.nii.gz' file_moco_params_y = 'moco_params_y.nii.gz' ext_data = '.nii.gz' # workaround "too many open files" by slurping the data # TODO: check if .nii can be used mat_final = 'mat_final' # ext_mat = 'Warp.nii.gz' # warping field # Start timer start_time = time.time() printv('\nInput parameters:', param.verbose) printv(' Input file ............ ' + param.fname_data, param.verbose) printv(' Group size ............ {}'.format(param.group_size), param.verbose) path_tmp = tmp_create(basename="moco") # Copying input data to tmp folder printv('\nCopying input data to tmp folder and convert to nii...', param.verbose) im_data = convert(Image(param.fname_data)) im_data.save(os.path.join(path_tmp, file_data), mutable=True, verbose=param.verbose) if param.fname_mask != '': im_mask = convert(Image(param.fname_mask)) im_mask.save(os.path.join(path_tmp, file_mask), mutable=True, verbose=param.verbose) # Update field in param (because used later in another function, and param class will be passed) param.fname_mask = file_mask if param.fname_bvals != '': _, _, ext_bvals = extract_fname(param.fname_bvals) file_bvals = f"bvals.{ext_bvals}" # Use hardcoded name to avoid potential duplicate files when copying copyfile(param.fname_bvals, os.path.join(path_tmp, file_bvals)) param.fname_bvals = file_bvals if param.fname_bvecs != '': _, _, ext_bvecs = extract_fname(param.fname_bvecs) file_bvecs = f"bvecs.{ext_bvecs}" # Use hardcoded name to avoid potential duplicate files when copying copyfile(param.fname_bvecs, os.path.join(path_tmp, file_bvecs)) param.fname_bvecs = file_bvecs # Build absolute output path and go to tmp folder curdir = os.getcwd() path_out_abs = os.path.abspath(param.path_out) os.chdir(path_tmp) # Get dimensions of data printv('\nGet dimensions of data...', param.verbose) nx, ny, nz, nt, px, py, pz, pt = im_data.dim printv(' ' + str(nx) + ' x ' + str(ny) + ' x ' + str(nz), param.verbose) # Get orientation printv('\nData orientation: ' + im_data.orientation, param.verbose) if im_data.orientation[2] in 'LR': param.is_sagittal = True printv(' Treated as sagittal') elif im_data.orientation[2] in 'IS': param.is_sagittal = False printv(' Treated as axial') else: param.is_sagittal = False printv( 'WARNING: Orientation seems to be neither axial nor sagittal. Treated as axial.' ) printv( "\nSet suffix of transformation file name, which depends on the orientation:" ) if param.is_sagittal: param.suffix_mat = '0GenericAffine.mat' printv( "Orientation is sagittal, suffix is '{}'. The image is split across the R-L direction, and the " "estimated transformation is a 2D affine transfo.".format( param.suffix_mat)) else: param.suffix_mat = 'Warp.nii.gz' printv( "Orientation is axial, suffix is '{}'. The estimated transformation is a 3D warping field, which is " "composed of a stack of 2D Tx-Ty transformations".format( param.suffix_mat)) # Adjust group size in case of sagittal scan if param.is_sagittal and param.group_size != 1: printv( 'For sagittal data group_size should be one for more robustness. Forcing group_size=1.', 1, 'warning') param.group_size = 1 if param.is_diffusion: # Identify b=0 and DWI images index_b0, index_dwi, nb_b0, nb_dwi = \ sct_dmri_separate_b0_and_dwi.identify_b0(param.fname_bvecs, param.fname_bvals, param.bval_min, param.verbose) # check if dmri and bvecs are the same size if not nb_b0 + nb_dwi == nt: printv( '\nERROR in ' + os.path.basename(__file__) + ': Size of data (' + str(nt) + ') and size of bvecs (' + str(nb_b0 + nb_dwi) + ') are not the same. Check your bvecs file.\n', 1, 'error') sys.exit(2) # ================================================================================================================== # Prepare data (mean/groups...) # ================================================================================================================== # Split into T dimension printv('\nSplit along T dimension...', param.verbose) im_data_split_list = split_data(im_data, 3) for im in im_data_split_list: x_dirname, x_basename, x_ext = extract_fname(im.absolutepath) im.absolutepath = os.path.join(x_dirname, x_basename + ".nii.gz") im.save() if param.is_diffusion: # Merge and average b=0 images printv('\nMerge and average b=0 data...', param.verbose) im_b0_list = [] for it in range(nb_b0): im_b0_list.append(im_data_split_list[index_b0[it]]) im_b0 = concat_data(im_b0_list, 3).save(file_b0, verbose=0) # Average across time im_b0.mean(dim=3).save(add_suffix(file_b0, '_mean')) n_moco = nb_dwi # set number of data to perform moco on (using grouping) index_moco = index_dwi # If not a diffusion scan, we will motion-correct all volumes else: n_moco = nt index_moco = list(range(0, nt)) nb_groups = int(math.floor(n_moco / param.group_size)) # Generate groups indexes group_indexes = [] for iGroup in range(nb_groups): group_indexes.append(index_moco[(iGroup * param.group_size):((iGroup + 1) * param.group_size)]) # add the remaining images to a new last group (in case the total number of image is not divisible by group_size) nb_remaining = n_moco % param.group_size # number of remaining images if nb_remaining > 0: nb_groups += 1 group_indexes.append(index_moco[len(index_moco) - nb_remaining:len(index_moco)]) _, file_dwi_basename, file_dwi_ext = extract_fname(file_datasub) # Group data list_file_group = [] for iGroup in sct_progress_bar(range(nb_groups), unit='iter', unit_scale=False, desc="Merge within groups", ascii=False, ncols=80): # get index index_moco_i = group_indexes[iGroup] n_moco_i = len(index_moco_i) # concatenate images across time, within this group file_dwi_merge_i = os.path.join(file_dwi_basename + '_' + str(iGroup) + ext_data) im_dwi_list = [] for it in range(n_moco_i): im_dwi_list.append(im_data_split_list[index_moco_i[it]]) im_dwi_out = concat_data(im_dwi_list, 3).save(file_dwi_merge_i, verbose=0) # Average across time list_file_group.append( os.path.join(file_dwi_basename + '_' + str(iGroup) + '_mean' + ext_data)) im_dwi_out.mean(dim=3).save(list_file_group[-1]) # Merge across groups printv('\nMerge across groups...', param.verbose) # file_dwi_groups_means_merge = 'dwi_averaged_groups' fname_dw_list = [] for iGroup in range(nb_groups): fname_dw_list.append(list_file_group[iGroup]) im_dw_list = [Image(fname) for fname in fname_dw_list] concat_data(im_dw_list, 3).save(file_datasubgroup, verbose=0) # Cleanup del im, im_data_split_list # ================================================================================================================== # Estimate moco # ================================================================================================================== # Initialize another class instance that will be passed on to the moco() function param_moco = deepcopy(param) if param.is_diffusion: # Estimate moco on b0 groups printv( '\n-------------------------------------------------------------------------------', param.verbose) printv(' Estimating motion on b=0 images...', param.verbose) printv( '-------------------------------------------------------------------------------', param.verbose) param_moco.file_data = 'b0.nii' # Identify target image if index_moco[0] != 0: # If first DWI is not the first volume (most common), then there is a least one b=0 image before. In that # case select it as the target image for registration of all b=0 param_moco.file_target = os.path.join( file_data_dirname, file_data_basename + '_T' + str(index_b0[index_moco[0] - 1]).zfill(4) + ext_data) else: # If first DWI is the first volume, then the target b=0 is the first b=0 from the index_b0. param_moco.file_target = os.path.join( file_data_dirname, file_data_basename + '_T' + str(index_b0[0]).zfill(4) + ext_data) # Run moco param_moco.path_out = '' param_moco.todo = 'estimate_and_apply' param_moco.mat_moco = 'mat_b0groups' file_mat_b0, _ = moco(param_moco) # Estimate moco across groups printv( '\n-------------------------------------------------------------------------------', param.verbose) printv(' Estimating motion across groups...', param.verbose) printv( '-------------------------------------------------------------------------------', param.verbose) param_moco.file_data = file_datasubgroup param_moco.file_target = list_file_group[ 0] # target is the first volume (closest to the first b=0 if DWI scan) param_moco.path_out = '' param_moco.todo = 'estimate_and_apply' param_moco.mat_moco = 'mat_groups' file_mat_datasub_group, _ = moco(param_moco) # Spline Regularization along T if param.spline_fitting: # TODO: fix this scenario (haven't touched that code for a while-- it is probably buggy) raise NotImplementedError() # spline(mat_final, nt, nz, param.verbose, np.array(index_b0), param.plot_graph) # ================================================================================================================== # Apply moco # ================================================================================================================== # If group_size>1, assign transformation to each individual ungrouped 3d volume if param.group_size > 1: file_mat_datasub = [] for iz in range(len(file_mat_datasub_group)): # duplicate by factor group_size the transformation file for each it # example: [mat.Z0000T0001Warp.nii] --> [mat.Z0000T0001Warp.nii, mat.Z0000T0001Warp.nii] for group_size=2 file_mat_datasub.append( functools.reduce(operator.iconcat, [[i] * param.group_size for i in file_mat_datasub_group[iz]], [])) else: file_mat_datasub = file_mat_datasub_group # Copy transformations to mat_final folder and rename them appropriately copy_mat_files(nt, file_mat_datasub, index_moco, mat_final, param) if param.is_diffusion: copy_mat_files(nt, file_mat_b0, index_b0, mat_final, param) # Apply moco on all dmri data printv( '\n-------------------------------------------------------------------------------', param.verbose) printv(' Apply moco', param.verbose) printv( '-------------------------------------------------------------------------------', param.verbose) param_moco.file_data = file_data param_moco.file_target = list_file_group[ 0] # reference for reslicing into proper coordinate system param_moco.path_out = '' # TODO not used in moco() param_moco.mat_moco = mat_final param_moco.todo = 'apply' file_mat_data, im_moco = moco(param_moco) # copy geometric information from header # NB: this is required because WarpImageMultiTransform in 2D mode wrongly sets pixdim(3) to "1". im_moco.header = im_data.header im_moco.save(verbose=0) # Average across time if param.is_diffusion: # generate b0_moco_mean and dwi_moco_mean args = [ '-i', im_moco.absolutepath, '-bvec', param.fname_bvecs, '-a', '1', '-v', '0' ] if not param.fname_bvals == '': # if bvals file is provided args += ['-bval', param.fname_bvals] fname_b0, fname_b0_mean, fname_dwi, fname_dwi_mean = sct_dmri_separate_b0_and_dwi.main( argv=args) else: fname_moco_mean = add_suffix(im_moco.absolutepath, '_mean') im_moco.mean(dim=3).save(fname_moco_mean) # Extract and output the motion parameters (doesn't work for sagittal orientation) printv('Extract motion parameters...') if param.output_motion_param: if param.is_sagittal: printv( 'Motion parameters cannot be generated for sagittal images.', 1, 'warning') else: files_warp_X, files_warp_Y = [], [] moco_param = [] for fname_warp in file_mat_data[0]: # Cropping the image to keep only one voxel in the XY plane im_warp = Image(fname_warp + param.suffix_mat) im_warp.data = np.expand_dims(np.expand_dims( im_warp.data[0, 0, :, :, :], axis=0), axis=0) # These three lines allow to generate one file instead of two, containing X, Y and Z moco parameters #fname_warp_crop = fname_warp + '_crop_' + ext_mat # files_warp.append(fname_warp_crop) # im_warp.save(fname_warp_crop) # Separating the three components and saving X and Y only (Z is equal to 0 by default). im_warp_XYZ = multicomponent_split(im_warp) fname_warp_crop_X = fname_warp + '_crop_X_' + param.suffix_mat im_warp_XYZ[0].save(fname_warp_crop_X) files_warp_X.append(fname_warp_crop_X) fname_warp_crop_Y = fname_warp + '_crop_Y_' + param.suffix_mat im_warp_XYZ[1].save(fname_warp_crop_Y) files_warp_Y.append(fname_warp_crop_Y) # Calculating the slice-wise average moco estimate to provide a QC file moco_param.append([ np.mean(np.ravel(im_warp_XYZ[0].data)), np.mean(np.ravel(im_warp_XYZ[1].data)) ]) # These two lines allow to generate one file instead of two, containing X, Y and Z moco parameters # im_warp = [Image(fname) for fname in files_warp] # im_warp_concat = concat_data(im_warp, dim=3) # im_warp_concat.save('fmri_moco_params.nii') # Concatenating the moco parameters into a time series for X and Y components. im_warp_X = [Image(fname) for fname in files_warp_X] im_warp_concat = concat_data(im_warp_X, dim=3) im_warp_concat.save(file_moco_params_x) im_warp_Y = [Image(fname) for fname in files_warp_Y] im_warp_concat = concat_data(im_warp_Y, dim=3) im_warp_concat.save(file_moco_params_y) # Writing a TSV file with the slicewise average estimate of the moco parameters. Useful for QC with open(file_moco_params_csv, 'wt', newline='') as out_file: tsv_writer = csv.writer(out_file, delimiter='\t') tsv_writer.writerow(['X', 'Y']) for mocop in moco_param: tsv_writer.writerow([mocop[0], mocop[1]]) # Generate output files printv('\nGenerate output files...', param.verbose) fname_moco = os.path.join( path_out_abs, add_suffix(os.path.basename(param.fname_data), param.suffix)) generate_output_file(im_moco.absolutepath, fname_moco) if param.is_diffusion: generate_output_file(fname_b0_mean, add_suffix(fname_moco, '_b0_mean')) generate_output_file(fname_dwi_mean, add_suffix(fname_moco, '_dwi_mean')) else: generate_output_file(fname_moco_mean, add_suffix(fname_moco, '_mean')) if os.path.exists(file_moco_params_csv): generate_output_file(file_moco_params_x, os.path.join(path_out_abs, file_moco_params_x), squeeze_data=False) generate_output_file(file_moco_params_y, os.path.join(path_out_abs, file_moco_params_y), squeeze_data=False) generate_output_file(file_moco_params_csv, os.path.join(path_out_abs, file_moco_params_csv)) # Delete temporary files if param.remove_temp_files == 1: printv('\nDelete temporary files...', param.verbose) rmtree(path_tmp, verbose=param.verbose) # come back to working directory os.chdir(curdir) # display elapsed time elapsed_time = time.time() - start_time printv('\nElapsed time: ' + str(int(np.round(elapsed_time))) + 's', param.verbose) fname_moco = os.path.join( param.path_out, add_suffix(os.path.basename(param.fname_data), param.suffix)) return fname_moco