def _start_merger(self):
'''Blocks until the two "subjobs" are done
(with new implementation, they will be done, before this is even called)
creates the merger job and puts it in the jobs queue,
cleans up the alignment subdirectories,
signals an event that signifies the fact that the merge job is on queue,
and then returns.
Called by wait()
'''
if self.killed:
raise RuntimeError("PastaAligner Job killed")
assert (self.subjob1 is not None)
result1 = self.subjob1.get_results()
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob1 = None
assert (self.subjob2 is not None)
result2 = self.subjob2.get_results()
self.subjob2 = None
if self.killed:
raise RuntimeError("PastaAligner Job killed")
assert (result1.get_num_loci() == result2.get_num_loci())
mj_list = []
for n, r1 in enumerate(result1):
r2 = result2[n]
cs = self.context_str + " merger" + str(n)
mj = self.pasta_team.merger.create_job(
r1,
r2,
tmp_dir_par=self.tmp_dir_par,
delete_temps=self.delete_temps,
context_str=cs)
mj.add_parent_tickable_job(self)
self.add_child(mj)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
mj_list.append(mj)
self.merge_job_list = mj_list
for mj in mj_list:
jobq.put(mj)
if self.delete_temps:
for d in self._dirs_to_cleanup:
self.pasta_team.temp_fs.remove_dir(d)
self._merge_queued_event.set()
def _start_merger(self):
'''Blocks until the two "subjobs" are done
(with new implementation, they will be done, before this is even called)
creates the merger job and puts it in the jobs queue,
cleans up the alignment subdirectories,
signals an event that signifies the fact that the merge job is on queue,
and then returns.
Called by wait()
'''
if self.killed:
raise RuntimeError("PastaAligner Job killed")
assert(self.subjob1 is not None)
result1 = self.subjob1.get_results()
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob1 = None
assert(self.subjob2 is not None)
result2 = self.subjob2.get_results()
self.subjob2 = None
if self.killed:
raise RuntimeError("PastaAligner Job killed")
assert(result1.get_num_loci() == result2.get_num_loci())
mj_list = []
for n, r1 in enumerate(result1):
r2 = result2[n]
cs = self.context_str + " merger" + str(n)
mj = self.pasta_team.merger.create_job(r1,
r2,
tmp_dir_par=self.tmp_dir_par,
delete_temps=self.delete_temps,
context_str=cs)
mj.add_parent_tickable_job(self)
self.add_child(mj)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
mj_list.append(mj)
self.merge_job_list = mj_list
for mj in mj_list:
jobq.put(mj)
if self.delete_temps:
for d in self._dirs_to_cleanup:
self.pasta_team.temp_fs.remove_dir(d)
self._merge_queued_event.set()
def run(self, tmp_dir_par, pasta_products=None):
assert(os.path.exists(tmp_dir_par))
self._reset_current_run_settings()
self._reset_jobs()
self.start_time = time.time()
self.last_improvement_time = self.start_time
num_non_update_iter = 0
configuration = self.configuration()
# Here we check if the max_subproblem_frac is more stringent than max_subproblem_size
frac_max = int(math.ceil(self.max_subproblem_frac*self.tree.n_leaves))
if frac_max > self.max_subproblem_size:
configuration['max_subproblem_size'] = frac_max
MESSENGER.send_info('Max subproblem set to {0}'.format(
configuration['max_subproblem_size']))
if configuration['max_subproblem_size'] >= self.tree.n_leaves:
MESSENGER.send_warning('''\n
WARNING: you have specified a max subproblem ({0}) that is equal to or greater
than the number of taxa ({0}). Thus, the PASTA algorithm will not be invoked
under the current configuration (i.e., no tree decomposition will occur).
If you did not intend for this behavior (which you probably did not since
you are using PASTA) please adjust your settings for the max subproblem and
try running PASTA again. If you intended to use PASTA to align your data with
the specified aligner tool *without* any decomposition, you can ignore this
message.\n'''.format(configuration['max_subproblem_size'],
self.tree.n_leaves))
if configuration['max_subproblem_size'] == 1:
MESSENGER.send_error(''' You have specified a max subproblem size of 1. PASTA requires a max subproblem size of at least 2. ''')
sys.exit(1)
delete_iteration_temps = not self.keep_iteration_temporaries
delete_realignment_temps = delete_iteration_temps or (not self.keep_realignment_temporaries)
configuration['delete_temps'] = delete_realignment_temps
while self._keep_iterating():
record_timestamp(os.path.join(tmp_dir_par, 'start_pastaiter_timestamp.txt'))
# create a subdirectory for this iteration
curr_iter_tmp_dir_par = os.path.join(tmp_dir_par, 'step' + str(self.current_iteration))
curr_iter_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(curr_iter_tmp_dir_par)
_LOG.debug('directory %s created' % curr_iter_tmp_dir_par)
break_strategy_index = 0
this_iter_score_improved = False
while True:
break_strategy = self._get_break_strategy(break_strategy_index)
if not bool(break_strategy):
break
context_str = "iter%d-%s" % (self.current_iteration, break_strategy)
# create a subdirectory for this iteration/break_strategy
curr_tmp_dir_par = os.path.join(curr_iter_tmp_dir_par, break_strategy)
curr_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(curr_tmp_dir_par)
record_timestamp(os.path.join(curr_tmp_dir_par, 'start_align_timestamp.txt'))
# Align (with decomposition...)
self.status('Step %d. Realigning with decomposition strategy set to %s' % (self.current_iteration, break_strategy))
if self.killed:
raise RuntimeError("PASTA Job killed")
tree_for_aligner = self.get_tree_copy()
aligner = PASTAAlignerJob(multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=tree_for_aligner,
tmp_base_dir=curr_tmp_dir_par,
reset_recursion_index=True,
skip_merge=self.pastamerge,
**configuration)
self.pasta_aligner_job = aligner
aligner.launch_alignment(break_strategy=break_strategy,
context_str=context_str)
if self.pastamerge:
_LOG.debug("Build PASTA merge jobs")
subsets_tree = self.build_subsets_tree(curr_tmp_dir_par)
if len(self.pasta_team.subsets.values()) == 1:
# can happen if there are no decompositions
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = self.pasta_team.subsets.values()[0].get_results()
else:
pariwise_tmp_dir_par = os.path.join(curr_tmp_dir_par, "pw")
pariwise_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(pariwise_tmp_dir_par)
pmj = PASTAMergerJob(multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=subsets_tree,
tmp_base_dir=pariwise_tmp_dir_par,
reset_recursion_index=True,
#delete_temps2=False,
**configuration)
pmj.launch_alignment(context_str=context_str)
# Start alignment jobs
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = pmj.get_results()
del pmj
self.pasta_team.alignmentjobs = []
self.pasta_team.subsets = {}
else:
new_multilocus_dataset = aligner.get_results()
_LOG.debug("Alignment obtained. Preparing for tree.")
self.pasta_aligner_job = None
del aligner
record_timestamp(os.path.join(curr_tmp_dir_par, 'start_treeinference_timestamp.txt'))
# Tree inference
if self.start_tree_search_from_current:
start_from = self.tree
else:
start_from = None
self.status('Step %d. Alignment obtained. Tree inference beginning...' % (self.current_iteration))
if self.killed:
raise RuntimeError("PASTA Job killed")
tbj = self.pasta_team.tree_estimator.create_job(new_multilocus_dataset,
starting_tree=start_from,
num_cpus=self.num_cpus,
context_str=context_str + " tree",
tmp_dir_par=curr_tmp_dir_par,
delete_temps=delete_iteration_temps,
pasta_products=pasta_products,
step_num=self.current_iteration,
mask_gappy_sites = self.mask_gappy_sites)
prev_curr_align = self.curr_iter_align_tmp_filename
prev_curr_tree = self.curr_iter_tree_tmp_filename
self.curr_iter_align_tmp_filename = pasta_products.get_abs_path_for_iter_output(self.current_iteration, TEMP_SEQ_ALIGNMENT_TAG, allow_existing=True)
self.curr_iter_tree_tmp_filename = pasta_products.get_abs_path_for_iter_output(self.current_iteration, TEMP_TREE_TAG, allow_existing=True)
self.tree_build_job = tbj
jobq.put(tbj)
new_score, new_tree_str = tbj.get_results()
self.tree_build_job = None
del tbj
if self.killed:
raise RuntimeError("PASTA Job killed")
record_timestamp(os.path.join(curr_tmp_dir_par, 'end_treeinference_timestamp.txt'))
curr_timestamp = time.time()
accept_iteration = False
if self.score is None:
self.score = new_score
if self.best_score is None or new_score > self.best_score:
self.store_optimum_results(new_multilocus_dataset,
new_tree_str,
new_score,
curr_timestamp)
this_iter_score_improved = True
accept_iteration = True
if self._get_accept_mode(new_score=new_score, break_strategy_index=break_strategy_index) == AcceptMode.BLIND_MODE:
if self.blind_mode_is_final:
self.is_stuck_in_blind = True
if self.switch_to_blind_timestamp is None:
if self._blindmode_trigger:
_LOG.debug("Blind runmode trigger = %s" % self._blindmode_trigger)
self.switch_to_blind_iter = self.current_iteration
self.switch_to_blind_timestamp = curr_timestamp
accept_iteration = True
if accept_iteration:
self.score = new_score
self.multilocus_dataset = new_multilocus_dataset
self.tree_str = new_tree_str
if this_iter_score_improved:
self.status('realignment accepted and score improved.')
else:
self.status('realignment accepted and despite the score not improving.')
# we do not want to continue to try different breaking strategies for this iteration so we break
self.status('current score: %s, best score: %s' % (self.score, self.best_score) )
break
else:
self.status('realignment NOT accepted.')
self.curr_iter_align_tmp_filename = prev_curr_align
self.curr_iter_tree_tmp_filename = prev_curr_tree
break_strategy_index += 1
# self.status('current score: %s, best score: %s' % (self.score, self.best_score) )
if not this_iter_score_improved:
self.num_iter_since_imp += 1
self.current_iteration += 1
if self._termination_trigger:
_LOG.debug("Termination trigger = %s" % self._termination_trigger)
record_timestamp(os.path.join(tmp_dir_par, 'end_pastaiter_timestamp.txt'))
### TODO: if configuration is 'return_final_iter_TreeAndAlignpair', then skip the following three lines
if not self.return_final_tree_and_alignment:
self.multilocus_dataset = self.best_multilocus_dataset.new_with_shared_meta()
for locus_alignment in self.best_multilocus_dataset:
self.multilocus_dataset.append(copy.copy(locus_alignment))
self.tree_str = self.best_tree_str
self.score = self.best_score
else:
assert self.multilocus_dataset is not None
assert self.tree_str is not None
assert self.score is not None
def run(self, tmp_dir_par, pasta_products=None):
assert (os.path.exists(tmp_dir_par))
self._reset_current_run_settings()
self._reset_jobs()
self.start_time = time.time()
self.last_improvement_time = self.start_time
num_non_update_iter = 0
configuration = self.configuration()
# Here we check if the max_subproblem_frac is more stringent than max_subproblem_size
frac_max = int(math.ceil(self.max_subproblem_frac *
self.tree.n_leaves))
if frac_max > self.max_subproblem_size:
configuration['max_subproblem_size'] = frac_max
MESSENGER.send_info('Max subproblem set to {0}'.format(
configuration['max_subproblem_size']))
if configuration['max_subproblem_size'] >= self.tree.n_leaves:
MESSENGER.send_warning('''\n
WARNING: you have specified a max subproblem ({0}) that is equal to or greater
than the number of taxa ({0}). Thus, the PASTA algorithm will not be invoked
under the current configuration (i.e., no tree decomposition will occur).
If you did not intend for this behavior (which you probably did not since
you are using PASTA) please adjust your settings for the max subproblem and
try running PASTA again. If you intended to use PASTA to align your data with
the specified aligner tool *without* any decomposition, you can ignore this
message.\n'''.format(configuration['max_subproblem_size'],
self.tree.n_leaves))
if configuration['max_subproblem_size'] == 1:
MESSENGER.send_error(
''' You have specified a max subproblem size of 1. PASTA requires a max subproblem size of at least 2. '''
)
sys.exit(1)
delete_iteration_temps = not self.keep_iteration_temporaries
delete_realignment_temps = delete_iteration_temps or (
not self.keep_realignment_temporaries)
configuration['delete_temps'] = delete_realignment_temps
while self._keep_iterating():
record_timestamp(
os.path.join(tmp_dir_par, 'start_pastaiter_timestamp.txt'))
# create a subdirectory for this iteration
curr_iter_tmp_dir_par = os.path.join(
tmp_dir_par, 'step' + str(self.current_iteration))
curr_iter_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(
curr_iter_tmp_dir_par)
_LOG.debug('directory %s created' % curr_iter_tmp_dir_par)
break_strategy_index = 0
this_iter_score_improved = False
while True:
break_strategy = self._get_break_strategy(break_strategy_index)
if not bool(break_strategy):
break
context_str = "iter%d-%s" % (self.current_iteration,
break_strategy)
# create a subdirectory for this iteration/break_strategy
curr_tmp_dir_par = os.path.join(curr_iter_tmp_dir_par,
break_strategy)
curr_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(
curr_tmp_dir_par)
record_timestamp(
os.path.join(curr_tmp_dir_par,
'start_align_timestamp.txt'))
# Align (with decomposition...)
self.status(
'Step %d. Realigning with decomposition strategy set to %s'
% (self.current_iteration, break_strategy))
if self.killed:
raise RuntimeError("PASTA Job killed")
tree_for_aligner = self.get_tree_copy()
aligner = PASTAAlignerJob(
multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=tree_for_aligner,
tmp_base_dir=curr_tmp_dir_par,
reset_recursion_index=True,
skip_merge=self.pastamerge,
**configuration)
self.pasta_aligner_job = aligner
aligner.launch_alignment(break_strategy=break_strategy,
context_str=context_str)
if self.pastamerge:
_LOG.debug("Build PASTA merge jobs")
subsets_tree = self.build_subsets_tree(
curr_tmp_dir_par, self.build_MST)
if len(self.pasta_team.subsets) == 1:
# can happen if there are no decompositions
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = list(
self.pasta_team.subsets.values())[0].get_results()
else:
pariwise_tmp_dir_par = os.path.join(
curr_tmp_dir_par, "pw")
pariwise_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(
pariwise_tmp_dir_par)
pmj = PASTAMergerJob(
multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=subsets_tree,
tmp_base_dir=pariwise_tmp_dir_par,
reset_recursion_index=True,
#delete_temps2=False,
**configuration)
pmj.launch_alignment(context_str=context_str)
# Start alignment jobs
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = pmj.get_results()
del pmj
self.pasta_team.alignmentjobs = []
self.pasta_team.subsets = {}
else:
new_multilocus_dataset = aligner.get_results()
_LOG.debug("Alignment obtained. Preparing for tree.")
self.pasta_aligner_job = None
del aligner
record_timestamp(
os.path.join(curr_tmp_dir_par,
'start_treeinference_timestamp.txt'))
# Tree inference
if self.start_tree_search_from_current:
start_from = self.tree
else:
start_from = None
self.status(
'Step %d. Alignment obtained. Tree inference beginning...'
% (self.current_iteration))
if self.killed:
raise RuntimeError("PASTA Job killed")
tbj = self.pasta_team.tree_estimator.create_job(
new_multilocus_dataset,
starting_tree=start_from,
num_cpus=self.num_cpus,
context_str=context_str + " tree",
tmp_dir_par=curr_tmp_dir_par,
delete_temps=delete_iteration_temps,
pasta_products=pasta_products,
step_num=self.current_iteration,
mask_gappy_sites=self.mask_gappy_sites)
prev_curr_align = self.curr_iter_align_tmp_filename
prev_curr_tree = self.curr_iter_tree_tmp_filename
self.curr_iter_align_tmp_filename = pasta_products.get_abs_path_for_iter_output(
self.current_iteration,
TEMP_SEQ_ALIGNMENT_TAG,
allow_existing=True)
self.curr_iter_tree_tmp_filename = pasta_products.get_abs_path_for_iter_output(
self.current_iteration, TEMP_TREE_TAG, allow_existing=True)
self.tree_build_job = tbj
jobq.put(tbj)
new_score, new_tree_str = tbj.get_results()
self.tree_build_job = None
del tbj
if self.killed:
raise RuntimeError("PASTA Job killed")
record_timestamp(
os.path.join(curr_tmp_dir_par,
'end_treeinference_timestamp.txt'))
curr_timestamp = time.time()
accept_iteration = False
if self.score is None:
self.score = new_score
if self.best_score is None or new_score > self.best_score:
self.store_optimum_results(new_multilocus_dataset,
new_tree_str, new_score,
curr_timestamp)
this_iter_score_improved = True
accept_iteration = True
if self._get_accept_mode(
new_score=new_score,
break_strategy_index=break_strategy_index
) == AcceptMode.BLIND_MODE:
if self.blind_mode_is_final:
self.is_stuck_in_blind = True
if self.switch_to_blind_timestamp is None:
if self._blindmode_trigger:
_LOG.debug("Blind runmode trigger = %s" %
self._blindmode_trigger)
self.switch_to_blind_iter = self.current_iteration
self.switch_to_blind_timestamp = curr_timestamp
accept_iteration = True
if accept_iteration:
self.score = new_score
self.multilocus_dataset = new_multilocus_dataset
self.tree_str = new_tree_str
if this_iter_score_improved:
self.status('realignment accepted and score improved.')
else:
self.status(
'realignment accepted and despite the score not improving.'
)
# we do not want to continue to try different breaking strategies for this iteration so we break
self.status('current score: %s, best score: %s' %
(self.score, self.best_score))
break
else:
self.status('realignment NOT accepted.')
self.curr_iter_align_tmp_filename = prev_curr_align
self.curr_iter_tree_tmp_filename = prev_curr_tree
break_strategy_index += 1
# self.status('current score: %s, best score: %s' % (self.score, self.best_score) )
if not this_iter_score_improved:
self.num_iter_since_imp += 1
self.current_iteration += 1
if self._termination_trigger:
_LOG.debug("Termination trigger = %s" % self._termination_trigger)
record_timestamp(
os.path.join(tmp_dir_par, 'end_pastaiter_timestamp.txt'))
### TODO: if configuration is 'return_final_iter_TreeAndAlignpair', then skip the following three lines
if not self.return_final_tree_and_alignment:
self.multilocus_dataset = self.best_multilocus_dataset.new_with_shared_meta(
)
for locus_alignment in self.best_multilocus_dataset:
self.multilocus_dataset.append(copy.copy(locus_alignment))
self.tree_str = self.best_tree_str
self.score = self.best_score
else:
assert self.multilocus_dataset is not None
assert self.tree_str is not None
assert self.score is not None
def run(self, *args, **kwargs):
start_worker(1)
job = self.create_job(*args, **kwargs)
jobq.put(job)
return job.get_results()
def launch_alignment(self,
tree=None,
break_strategy=None,
context_str=None):
'''Puts a alignment job(s) in the queue and then return None
get_results() must be called to get the alignment. Note that this call
may not be trivial in terms of time (the tree will be decomposed, lots
of temporary files may be written...), but the call does not block until
completion of the alignments.
Rather it queues the alignment jobs so that multiple processors can be
exploited if they are available.
'''
if self.killed:
raise RuntimeError("PastaAligner Job killed")
if break_strategy is not None:
self.break_strategy = break_strategy
break_strategy = self.break_strategy
if tree is not None:
self.tree = tree
self.expected_number_of_taxa = self.multilocus_dataset.get_num_taxa(
) # for debugging purposes
self._reset_jobs()
prefix = "self.multilocus_dataset.get_num_taxa = %d" % self.expected_number_of_taxa
self.context_str = context_str
if self.context_str is None:
self.context_str = ''
_LOG.debug(
"Comparing expected_number_of_taxa=%d and max_subproblem_size=%d\n"
% (self.expected_number_of_taxa, self.max_subproblem_size))
if self.expected_number_of_taxa <= self.max_subproblem_size:
_LOG.debug("%s...Calling Aligner" % prefix)
aj_list = []
for index, single_locus_sd in enumerate(self.multilocus_dataset):
aj = self.pasta_team.aligner.create_job(
single_locus_sd,
tmp_dir_par=self.tmp_dir_par,
delete_temps=self.delete_temps,
context_str=self.context_str + " align" + str(index))
aj.add_parent_tickable_job(self)
self.add_child(aj)
aj_list.append(aj)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.pasta_team.alignmentjobs.append(aj)
self.align_job_list = aj_list
if self.skip_merge:
for taxa in self.tree.leaf_node_names():
self.pasta_team.subsets[taxa] = self
else:
for aj in aj_list:
jobq.put(aj)
else:
# added by uym2 on August 1st 2017
subjob1, subjob2 = self.bipartition_by_tree(break_strategy)
if subjob1 is None or subjob2 is None:
return
_LOG.debug("%s...Recursing" % prefix)
# create the subjobs
# the next line was modified by uym2 (August 1st 2017)
self.subjob1 = subjob1
self.subjob2 = subjob2
# store this dir so we can use it in the merger
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob1.add_parent(self)
self.subjob2.add_parent(self)
self.add_child(self.subjob1)
self.add_child(self.subjob2)
self.subjob1.launch_alignment(break_strategy=break_strategy)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob2.launch_alignment(break_strategy=break_strategy)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
def run(self, tmp_dir_par, pasta_products=None):
'''
This is to be called from the main pasta method ONLY
'''
if self.tmp_dir_par==None:
self.tmp_dir_par=tmp_dir_par
else:
tmp_dir_par=self.tmp_dir_par
configuration, delete_iteration_temps=self.run_start(tmp_dir_par, pasta_products)
# self.resumable=False
while self._keep_iterating():
if self.resumable==False:
record_timestamp(os.path.join(tmp_dir_par, 'start_pastaiter_timestamp.txt'))
# create a subdirectory for this iteration
curr_iter_tmp_dir_par = os.path.join(tmp_dir_par, 'step' + str(self.current_iteration))
curr_iter_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(curr_iter_tmp_dir_par)
_LOG.debug('directory %s created' % curr_iter_tmp_dir_par)
self.break_strategy_index = 0
this_iter_score_improved = False
break_strategy = self._get_break_strategy(self.break_strategy_index)
if not bool(break_strategy):
print "breaking from PastaInteruptableJob..."
break
context_str = "iter%d-%s" % (self.current_iteration, break_strategy)
self.context_str=context_str
# create a subdirectory for this iteration/break_strategy
curr_tmp_dir_par = os.path.join(curr_iter_tmp_dir_par, break_strategy)
curr_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(curr_tmp_dir_par)
self.curr_tmp_dir_par=curr_tmp_dir_par
record_timestamp(os.path.join(curr_tmp_dir_par, 'start_align_timestamp.txt'))
# Align (with decomposition...)
self.status('Step %d. Realigning with decomposition strategy set to %s' % (self.current_iteration, break_strategy))
if self.killed:
raise RuntimeError("PASTA Job killed")
tree_for_aligner = self.get_tree_copy()
aligner = PASTAInterruptibleAlignerJob(multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=tree_for_aligner,
tmp_base_dir=curr_tmp_dir_par,
reset_recursion_index=True,
skip_merge=self.pastamerge,
**configuration)
self.pasta_aligner_job = aligner
aligner.launch_alignment(break_strategy=break_strategy,
context_str=context_str)
# write jobs list
aln_job_list=pasta_products.get_abs_path_for_iter_output(self.current_iteration,'alnjoblist.txt')
aln_job_list_file=open(aln_job_list,'w')
self.aln_job_list_dict=[]
for aj in self.pasta_team.alignmentjobs:
self.aln_job_list_dict.append({
'file_read_job':True,
'alignedfn':aj.alignedfn,
'seqfn': aj.seqfn,
'scratch_dir': aj.scratch_dir,
'datatype': aj.datatype,
'context_str': aj.context_str
})
aln_job_list_file.write('%s,%s,%s\n' % (aj.scratch_dir, aj.seqfn, aj.alignedfn))
aln_job_list_file.close()
self.resumable=True
# self.pasta_team.alignmentjobs=[]
return aln_job_list, self.resumable
else:
# for ajd in self.aln_job_list_dict:
# self.pasta_team.alignmentjobs.append(self.pasta_team.aligner.create_file_read_job(**ajd))
# for aj in self.pasta_team.alignmentjobs:
# jobq.put(aj)
self.resumable=False
if self.resumable==False:
# re-initialize some variables from earlier
aligner=self.pasta_aligner_job
curr_tmp_dir_par=self.curr_tmp_dir_par
context_str=self.context_str
if self.pastamerge:
# pdb.set_trace()
_LOG.debug("Build PASTA merge jobs")
subsets_tree = self.build_subsets_tree(curr_tmp_dir_par)
if len(self.pasta_team.subsets.values()) == 1:
# can happen if there are no decompositions
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = self.pasta_team.subsets.values()[0].get_results()
else:
pariwise_tmp_dir_par = os.path.join(curr_tmp_dir_par, "pw")
pariwise_tmp_dir_par = self.pasta_team.temp_fs.create_subdir(pariwise_tmp_dir_par)
pmj = PASTAMergerJob(multilocus_dataset=self.multilocus_dataset,
pasta_team=self.pasta_team,
tree=subsets_tree,
tmp_base_dir=pariwise_tmp_dir_par,
reset_recursion_index=True,
#delete_temps2=False,
**configuration)
pmj.launch_alignment(context_str=context_str)
# Start alignment jobs
for job in self.pasta_team.alignmentjobs:
jobq.put(job)
new_multilocus_dataset = pmj.get_results()
del pmj
self.pasta_team.alignmentjobs = []
self.pasta_team.subsets = {}
else:
new_multilocus_dataset = aligner.get_results()
_LOG.debug("Alignment obtained. Preparing for tree.")
self.pasta_aligner_job = None
del aligner
record_timestamp(os.path.join(curr_tmp_dir_par, 'start_treeinference_timestamp.txt'))
# Tree inference
if self.start_tree_search_from_current:
start_from = self.tree
else:
start_from = None
self.status('Step %d. Alignment obtained. Tree inference beginning...' % (self.current_iteration))
if self.killed:
raise RuntimeError("PASTA Job killed")
tbj = self.pasta_team.tree_estimator.create_job(new_multilocus_dataset,
starting_tree=start_from,
num_cpus=self.num_cpus,
context_str=context_str + " tree",
tmp_dir_par=curr_tmp_dir_par,
delete_temps=delete_iteration_temps,
pasta_products=pasta_products,
step_num=self.current_iteration,
mask_gappy_sites = self.mask_gappy_sites)
prev_curr_align = self.curr_iter_align_tmp_filename
prev_curr_tree = self.curr_iter_tree_tmp_filename
self.curr_iter_align_tmp_filename = pasta_products.get_abs_path_for_iter_output(self.current_iteration, TEMP_SEQ_ALIGNMENT_TAG, allow_existing=True)
self.curr_iter_tree_tmp_filename = pasta_products.get_abs_path_for_iter_output(self.current_iteration, TEMP_TREE_TAG, allow_existing=True)
self.tree_build_job = tbj
jobq.put(tbj)
new_score, new_tree_str = tbj.get_results()
self.tree_build_job = None
del tbj
if self.killed:
raise RuntimeError("PASTA Job killed")
record_timestamp(os.path.join(curr_tmp_dir_par, 'end_treeinference_timestamp.txt'))
curr_timestamp = time.time()
accept_iteration = False
if self.score is None:
self.score = new_score
if self.best_score is None or new_score > self.best_score:
self.store_optimum_results(new_multilocus_dataset,
new_tree_str,
new_score,
curr_timestamp)
this_iter_score_improved = True
accept_iteration = True
if self._get_accept_mode(new_score=new_score, break_strategy_index=self.break_strategy_index) == AcceptMode.BLIND_MODE:
if self.blind_mode_is_final:
self.is_stuck_in_blind = True
if self.switch_to_blind_timestamp is None:
if self._blindmode_trigger:
_LOG.debug("Blind runmode trigger = %s" % self._blindmode_trigger)
self.switch_to_blind_iter = self.current_iteration
self.switch_to_blind_timestamp = curr_timestamp
accept_iteration = True
if accept_iteration:
self.score = new_score
self.multilocus_dataset = new_multilocus_dataset
self.tree_str = new_tree_str
if this_iter_score_improved:
self.status('realignment accepted and score improved.')
else:
self.status('realignment accepted and despite the score not improving.')
# we do not want to continue to try different breaking strategies for this iteration so we break
self.status('current score: %s, best score: %s' % (self.score, self.best_score) )
# break
else:
self.status('realignment NOT accepted.')
self.curr_iter_align_tmp_filename = prev_curr_align
self.curr_iter_tree_tmp_filename = prev_curr_tree
# break_strategy_index += 1
# self.status('current score: %s, best score: %s' % (self.score, self.best_score) )
if not this_iter_score_improved:
self.num_iter_since_imp += 1
self.current_iteration += 1
if self.resumable==False:
if self._termination_trigger:
_LOG.debug("Termination trigger = %s" % self._termination_trigger)
record_timestamp(os.path.join(tmp_dir_par, 'end_pastaiter_timestamp.txt'))
### TODO: if configuration is 'return_final_iter_TreeAndAlignpair', then skip the following three lines
if not self.return_final_tree_and_alignment:
self.multilocus_dataset = self.best_multilocus_dataset.new_with_shared_meta()
for locus_alignment in self.best_multilocus_dataset:
self.multilocus_dataset.append(copy.copy(locus_alignment))
self.tree_str = self.best_tree_str
self.score = self.best_score
else:
assert self.multilocus_dataset is not None
assert self.tree_str is not None
assert self.score is not None
return (None, None)
def launch_alignment(self, tree=None, break_strategy=None, context_str=None):
'''Puts a alignment job(s) in the queue and then return None
get_results() must be called to get the alignment. Note that this call
may not be trivial in terms of time (the tree will be decomposed, lots
of temporary files may be written...), but the call does not block until
completion of the alignments.
Rather it queues the alignment jobs so that multiple processors can be
exploited if they are available.
'''
if self.killed:
raise RuntimeError("PastaAligner Job killed")
if break_strategy is not None:
self.break_strategy = break_strategy
break_strategy = self.break_strategy
if tree is not None:
self.tree = tree
self.expected_number_of_taxa = self.multilocus_dataset.get_num_taxa() # for debugging purposes
self._reset_jobs()
prefix = "self.multilocus_dataset.get_num_taxa = %d" % self.expected_number_of_taxa
self.context_str = context_str
if self.context_str is None:
self.context_str = ''
_LOG.debug("Comparing expected_number_of_taxa=%d and max_subproblem_size=%d\n" % (self.expected_number_of_taxa, self.max_subproblem_size))
if self.expected_number_of_taxa <= self.max_subproblem_size:
_LOG.debug("%s...Calling Aligner" % prefix)
aj_list = []
for index, single_locus_sd in enumerate(self.multilocus_dataset):
aj = self.pasta_team.aligner.create_job(single_locus_sd,
tmp_dir_par=self.tmp_dir_par,
delete_temps=self.delete_temps,
context_str=self.context_str + " align" + str(index))
aj.add_parent_tickable_job(self)
self.add_child(aj)
aj_list.append(aj)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.pasta_team.alignmentjobs.append(aj)
self.align_job_list = aj_list
if self.skip_merge:
for taxa in self.tree.leaf_node_names():
self.pasta_team.subsets[taxa]=self
else:
for aj in aj_list:
jobq.put(aj)
else:
_LOG.debug("%s...Recursing" % prefix)
# create the subjobs
self.subjob1, self.subjob2 = self.bipartition_by_tree(break_strategy)
# store this dir so we can use it in the merger
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob1.add_parent(self)
self.subjob2.add_parent(self)
self.add_child(self.subjob1)
self.add_child(self.subjob2)
self.subjob1.launch_alignment(break_strategy=break_strategy)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
self.subjob2.launch_alignment(break_strategy=break_strategy)
if self.killed:
raise RuntimeError("PastaAligner Job killed")
return
def run(self, *args, **kwargs):
start_worker(1)
job = self.create_job(*args, **kwargs)
jobq.put(job)
return job.get_results()