- It include many useful modules.
- It is easy to run these modules seperately, in order to reach your specific goal.
- It also can be used to run whole modules from RNA-seq data to any annotation information.
usage: annogesic [-h] [--version]
{create,get_input_files,get_target_fasta,annotation_transfer,expression_analysis,tsspredator,optimize_tsspredator,color_png,terminator,transcript_assembly,utr,srna,sorf,promoter,operon,circrna,go_term,srna_target,snp,ppi_network,subcellular_localization,riboswitch,screenshot}
...
positional arguments:
{create,get_input_files,get_target_fasta,annotation_transfer,expression_analysis,tsspredator,optimize_tsspredator,color_png,terminator,transcript_assembly,utr,srna,sorf,promoter,operon,circrna,go_term,srna_target,snp,ppi_network,subcellular_localization,riboswitch,screenshot}
commands
create Create a project
get_input_files Get required files. (i.e. annotation files, fasta
files)
get_target_fasta Get target fasta.
annotation_transfer
Run RATT to transfer the annotation files from
reference to target.
tsspredator Run TSSpredator to predict TSSs or processing sites.
optimize_tsspredator
Optimize TSSpredator based on (partial)manual detect
one.
color_png Generating color screenshots of TSS or processing
site. It only works after running batch script.
terminator Detect Terminators.
transcript_assembly
Run transcriptome assembly for detecting transcripts.
utr Run UTR detection to detect 5'UTR and 3'UTR.
srna Run sRNA detection to detect sRNA candidates.
sorf Run sORF detection to detect sORF candidates which has
expression.
promoter Run MEME to dicover promoter.
operon Detect operon and combine features together.
circrna Detect circular RNA.
go_term Extract and find Go terms.
srna_target sRNA target prediction.
snp Detection of SNP of transcripts.
ppi_network Generate protein-protein interaction with literature
supported.
subcellular_localization
Prediction of subcellular localization of genomic CDS.
riboswitch Prediction of riboswitch.
screenshot Generate screenshot for selected feature.
optional arguments:
-h, --help show this help message and exit
--version, -v show version