Client for sh-miR Designer RESTful API

Before usage you need to set up url in settings.py!

Example usage:

Fold sequence via mfold:

./shmir_client.py mfold ACTGAUUUGAC

Create sh-miR from one siRNA strand (active) or two siRNa strands separated by space.
First strand is active, both are in 5-3 orientation

./shmir_client.py from_sirna UUUGUAUUCGCCCUAGCGC CGCUAUGGCGAAUACAAACA

Create sh-miR from transcript name.
Optional parameters

• --min_gc : Minimal "GC" content in strand -- default: 40
• --max_gc : Maximal "GC" content in strand -- default: 60
• --max_offtarget : Maximal offtarget of strand -- default: 10
• --mirna_name : The name of miRNA backbone to use -- default: "all"
• --immuno : One of ["yes", "no", "no_difference"] -- default: "no_difference"

./shmir_client.py from_transcript NM_001618.3 --mirna_name miR-30a --min_gc 22