The scripts in this repository correspond to the following publication:

Ti, Alushin, Kapoor. "Human B-tubulin isotypes regulate microtubule protofilament number and stability". Developmental Cell, 2018.

A detailed protocol is forthcoming. In the meantime, please email galushin AT rockefeller.edu for guidance on their usage.

General requirements:

Frealign v9.11

EMAN2/SPARX

SPIDER v21.11 (includes radon transform operations), for diameter measurements of class averages

For 3D reconstructions, we picked and pre-processed our data in RELION through the 2D class average stage.