The Pse-in-One is able to generate totally 28 different features, including 14 features for DNA sequences, 6 features for RNA sequences, and 8 features for protein sequences. All these features can be deemed as different pseudo components.

To the best of our knowledge, Pse-in-One is so far the first web server that can generate all the possible pseudo components for DNA, RNA, and protein sequences, and even those defined by users themselves, and hence it is extremely flexible.

This Pse-in-One stand-alone version is open source software made available under GPL license. Please see the LICENSE file for further details.

• Home-Page: http://bioinformatics.hitsz.edu.cn/Pse-in-One/
• Official stand-alone source code repo: https://github.com/liufule12/Pse-in-One
• HTML document: http://bioinformatics.hitsz.edu.cn/Pse-in-One/doc/
• Issue tracker: https://github.com/liufule12/Pse-in-One/issues

Pse-in-One is tested to work under Python 2.6, Python 2.7, and Python 3.4.

You just need execute the corresponding file in command line environment.

For example: kmer.py test.txt output_kmer.txt 2 DNA

Please see the Pse-in-One_manual.pdf for more information. :D