The DNA_FT.py script will read all sequences in the input fasta file (in this case test.fasta), and generate the Fourier spectrum plot for each fasta entry. The plots for each entry are saved as .png files in the output directory specified with -o.

All dependencies are included in the dna_ft.yml file.
Build and activate the conda environment:

conda env create -f dna_ft.yml 
conda activate dna_ft

Then run the DNA_FT.py script:

python python DNA_FT.py -i test.fasta -o ./output/

It's possible to generate an overlapping plot for two fasta files (each with a single record - if there are multiple records, only the first will be considered from each file). For this, use the Compare_DNA_FT.py script:

python Compare_DNA_FT.py -f1 NC_045512.2.fasta -f2 MT066176.1.fasta -o output

The code uses the method outlined in the following publication:

Tiwari, S., Ramachandran, S., Bhattacharya, A., Bhattacharya, S. & Ramaswamy, R. Prediction of probable genes by Fourier analysis of genomic sequences. Comput. Appl. Biosci. 13, 263–270 (1997).