def __init__(self,organisms,
ncpus=1,evalue=1e-10,
recover=False,prefix='',
matrix='BLOSUM80',queue=Queue.Queue()):
CommonMultiProcess.__init__(self,ncpus,queue)
# Blast
self.organisms = list(organisms)
self.dbs = {}
self._prot2orgs = {}
self.out = []
self.evalue = float(evalue)
# TODO: implement recovery
self.recover = recover
#
self.results = {}
self._blast = Blaster()
self._pangenomeroom = None
self.prefix = prefix.rstrip('_')
self.matrix = matrix
self._already = set()
# Results
self.orthologs = {}
self.core = []
self.accessory = []
self.unique = []
class PanGenomer(CommonMultiProcess):
'''
Class panGenomer
'''
_statusDesc = {0:'Not started',
1:'Making room',
2:'Creating Blast DBs',
3:'Running Blast BBHs',
4:'Crafting the PanGenome',
5:'Cleaning up'}
_substatuses = [2,3]
def __init__(self,organisms,
ncpus=1,evalue=1e-10,
recover=False,prefix='',
matrix='BLOSUM80',queue=Queue.Queue()):
CommonMultiProcess.__init__(self,ncpus,queue)
# Blast
self.organisms = list(organisms)
self.dbs = {}
self._prot2orgs = {}
self.out = []
self.evalue = float(evalue)
# TODO: implement recovery
self.recover = recover
#
self.results = {}
self._blast = Blaster()
self._pangenomeroom = None
self.prefix = prefix.rstrip('_')
self.matrix = matrix
self._already = set()
# Results
self.orthologs = {}
self.core = []
self.accessory = []
self.unique = []
def makeRoom(self,location=''):
'''
Creates a tmp directory in the desired location
'''
try:
path = os.path.abspath(location)
path = os.path.join(path, 'tmp')
try:os.mkdir(path)
except:pass
path = os.path.join(path, 'pangenomeDBs')
self._room = path
os.mkdir(path)
except:
logger.debug('Temporary directory creation failed! %s'
%path)
try:
path = os.path.abspath(location)
path = os.path.join(path, 'tmp')
try:os.mkdir(path)
except:pass
path = os.path.join(path, 'pangenome')
self._pangenomeroom = path
os.mkdir(path)
except:
logger.debug('Temporary directory creation failed! %s'
%path)
def cleanUp(self):
'''
Removes the temporary directory
'''
shutil.rmtree(self._room, True)
shutil.rmtree(self._pangenomeroom, True)
def createDB(self):
dbindex = 0
self._maxsubstatus = len(self.organisms)
for org in self.organisms:
self._substatus += 1
self.updateStatus(sub=True)
seqs = [seq.id for seq in SeqIO.parse(open(org),'fasta')]
for seqid in seqs:
if seqid in self._prot2orgs:
logger.warning('Protein %s present as duplicate!'%seqid)
return False
self._prot2orgs[seqid] = org
self.dbs[org] = os.path.join(self._room,str(dbindex))
res = self._blast.createDB(org, 'prot', self.dbs[org])
if not res:
logger.error('Could not create DB for %s'%org)
return False
dbindex += 1
return True
def serialBBH(self):
orthindex = 1
self._maxsubstatus = len(self._prot2orgs)
for org in self.organisms:
seqs = [seq for seq in SeqIO.parse(open(org),'fasta')]
# Iterate over each protein
for seq in seqs:
self._substatus += 1
self.updateStatus(sub=True)
if seq.id in self._already:
continue
orthname = self.prefix + str(orthindex)
orgsincluded = [org]
self.orthologs[orthname] = [seq.id]
query = os.path.join(self._pangenomeroom,str(self._substatus))
if SeqIO.write([seq], open(query,'w'), 'fasta') <= 0:
logger.error('Error writing sequence %s to file'%seq.id)
return False
self.initiateParallel()
# Iterate over each other organism
for otherorg in self.organisms:
if org == otherorg:
continue
# Go fot it!
if len(seq) < 30:
short = True
else:
short = False
uniqueid = self.getUniqueID()
# Multi process
obj = RunBBH(query,seq.id,self.dbs[org],
self.dbs[otherorg],otherorg,
self.evalue,self.matrix,short=short,
uniqueid=uniqueid)
self._paralleltasks.put(obj)
# Poison pill to stop the workers
self.addPoison()
while True:
if self.killed:
logger.debug('Exiting for a kill signal')
return
while not self._parallelresults.empty():
if self.killed:
logger.debug('Exiting for a kill signal')
return
result = self._parallelresults.get()
if not result[2]:
logger.error('An error occurred for BBH on query %s'%seq.id+
' and target %s'%result[1])
return False
if result[0] and result[0] not in self._already:
self.orthologs[orthname].append(result[0])
orgsincluded.append(result[1])
self._already.add(result[0])
if self.isTerminated():
break
self.sleeper.sleep(0.1)
while not self._parallelresults.empty():
if self.killed:
logger.debug('Exiting for a kill signal')
return
result = self._parallelresults.get()
if not result[2]:
logger.error('An error occurred for BBH on query %s'%seq.id+
' and target %s'%result[1])
return False
if result[0] and result[0] not in self._already:
self.orthologs[orthname].append(result[0])
orgsincluded.append(result[1])
self._already.add(result[0])
self.killParallel()
if len(orgsincluded) < len(self.organisms):
logger.debug('Additional search on missing organisms for'+
' ortholog %s'%orthname)
for otherprotein in self.orthologs[orthname]:
if otherprotein == seq.id:
continue
neworg = self._prot2orgs[otherprotein]
if neworg == org:
continue
bFound = False
for seq in SeqIO.parse(open(neworg),'fasta'):
if seq.id == otherprotein:
bFound = True
break
if not bFound:
logger.error('%s not found!'%otherprotein)
return False
if SeqIO.write([seq], open(query,'w'), 'fasta') == 0:
logger.error('Error writing sequence %s to file'
%seq.id)
return False
self.initiateParallel()
for evenneworg in self.organisms:
if evenneworg in orgsincluded:
continue
# Go fot it!
if len(seq) < 30:
short = True
else:
short = False
uniqueid = self.getUniqueID()
# Multi process
obj = RunBBH(query,otherprotein,self.dbs[neworg],
self.dbs[evenneworg],evenneworg,
self.evalue,self.matrix,short=short,uniqueid=uniqueid)
self._paralleltasks.put(obj)
# Poison pill to stop the workers
self.addPoison()
while True:
if self.killed:
logger.debug('Exiting for a kill signal')
return
while not self._parallelresults.empty():
if self.killed:
logger.debug('Exiting for a kill signal')
return
result = self._parallelresults.get()
if not result[2]:
logger.error('An error occurred for BBH on query %s'%seq.id+
' and target %s'%result[1])
return False
if result[0] and result[0] not in self._already:
self.orthologs[orthname].append(result[0])
orgsincluded.append(result[1])
self._already.add(result[0])
if self.isTerminated():
break
self.sleeper.sleep(0.1)
while not self._parallelresults.empty():
if self.killed:
logger.debug('Exiting for a kill signal')
return
result = self._parallelresults.get()
if not result[2]:
logger.error('An error occurred for BBH on query %s'%seq.id+
' and target %s'%result[1])
return False
if result[0] and result[0] not in self._already:
self.orthologs[orthname].append(result[0])
orgsincluded.append(result[1])
self._already.add(result[0])
self.killParallel()
os.remove(query)
orthindex += 1
return True
def packPanGenome(self):
for g in self.orthologs:
if len(self.orthologs[g]) == len(self.organisms):
self.core.append(g)
elif len(self.orthologs[g]) == 1:
self.unique.append(g)
else:
self.accessory.append(g)
def run(self):
self.updateStatus()
self.makeRoom()
if self.killed:
return
self.updateStatus()
if not self.createDB():
self.sendFailure('Create DBs failure!')
self.cleanUp()
return
self.resetSubStatus()
if self.killed:
return
self.updateStatus()
if not self.serialBBH():
self.sendFailure('Serial BBH failure!')
self.killParallel()
self.cleanUp()
return
self.resetSubStatus()
if self.killed:
return
self.updateStatus()
self.packPanGenome()
if self.killed:
return
self.updateStatus()