def get_pore(img, percentile=75,edge_object_margin=1):
"""Extracts largest, non-border, darkest region from subimage - in context presumed to be the pore. Works out the
values are 0.
:param img: input image (section of full intensity leaf image with just stomate in it)
:type img: numpy.ndarray
:param percentile: the percentile of the intensity histogram below which values will be taken to be dark.
:type percentile: int
:param edge_object_margin: the width of border in which objects must lie to be removed as border objects, default = 1
:return: binary numpy.ndaarray
"""
dark_level = np.percentile(img, percentile )
#imshow(img)
cp = np.copy(img)
cp[img > dark_level] = 0
cp[img <= dark_level] = 1
label_objects, nb_labels = ndimage.label(cp)
removed = np.copy(label_objects)
for o in edge_objects(label_objects, margin=edge_object_margin):
removed[removed == o] = 0
new_label_objects, new_nb_labels = ndimage.label(removed)
props = measure.regionprops(new_label_objects, intensity_image=img)
if len(props) > 0:
area = max([i.area for i in props])
biggest = [i for i, j in enumerate(props) if j.area == area]
index_of_obj_to_keep = biggest[0]
label_of_obj_to_keep = np.arange(1, new_nb_labels + 1)[index_of_obj_to_keep]
new_label_objects[new_label_objects != label_of_obj_to_keep] = 0
final_label_objects, final_nb_labels = ndimage.label(new_label_objects)
return(final_label_objects)
else: #no pore
return(None)
def test_prediction_activity(out_dir, calcium_data, logged_ssvm_path, which_gt='gt', feats_unary='feats_pm', feats_pairwise='feats_xcorr_green'):
img_x, img_y = 512, 512
logger = pystruct_logger(logged_ssvm_path)
ssvm = logger.load()
print ssvm
print "Build edge graph"
edges = create_2d_edge_graph(img_x, img_y)
for sub in calcium_data.subvolumes:
print 'Preparing output directory for', sub.name
out_folder = 'test_prediction_activity/%s/' % sub.name
create_out_path(out_path=out_dir, except_on_exist=False)
print 'Loading stuff for subvolume', sub.name
gt = sub.get(which_gt).astype('uint8')
unary_feats = sub.get(feats_unary)
pairwise_feats = sub.get(feats_pairwise)
x_single = (unary_feats, edges, pairwise_feats)
y_single = gt.reshape(img_x * img_y)
print "\tPredicting on subvolume"
pred=ssvm.predict([x_single])
pred=pred[0].reshape(img_x, img_y)
print '\tScoring subvolume prediction'
score = ssvm.score([x_single], [y_single])
print 'Score on subvolume:', score
print 'Getting connected components'
cc_pred, cnt_obj_pred = ndimage.label(pred)
cc_gt, cnt_obj_gt = ndimage.label(gt)
print 'cc:', cnt_obj_pred
print 'Saving vizzzz'
for cc in xrange(1, cnt_obj_pred + 1):
label_indices_xy = np.where(cc_pred == cc)
tmp = np.zeros_like(gt)
tmp[label_indices_xy] = 1
tmp = tmp.reshape(img_x * img_y)
label_indices_rshp = np.where(tmp == 1)
label_indices_xy = np.asarray(label_indices_xy)
label_polygons_pppx = sub.mpl_get_polygon_patches(['active_very_certain', 'active_mod_certain', 'uncertain'])
label_polygons_ppp = sub.mpl_get_polygon_patches(['active_very_certain', 'active_mod_certain'])
label_polygons_pp = sub.mpl_get_polygon_patches(['active_very_certain'])
label_polygons = [label_polygons_pp, label_polygons_ppp, label_polygons_pppx]
roi = Roi(indices=label_indices_rshp, xy=label_indices_xy, subvolume=sub, janelia_id=cc,
meta_info='Spike trains of predicted neurons for %s (score: %.02f)' % (sub.name, score))
fig = roi.plot_like_annotation_labeler(prediction_image=pred, label_polygons=label_polygons)
plt.savefig(out_dir + '%d.png' % roi.id, dpi=600)
plt.close()
plt.imsave(out_dir + 'cc_pred.png', cc_pred)
plt.imsave(out_dir + 'cc_gt.png', cc_gt)
print '\n============================================\n'
print 'Done.'
def multithreshold(img,ignore_zeros=True,firstThreshold=20,nrThresholds=5):
'''
labeled,N = multithreshold(img, ignore_zeros = True)
Performs multi thresholding (which is a form of oversegmentation).
labeled is of the same size and type as img and contains different labels for
the N detected objects (the return of this function is similar to that of scipy.ndimage.label())
@param img: The input image
@param ignore_zeros: Don't take zero pixels into account
'''
output=numpy.zeros_like(img)
if ignore_zeros:
pmin=nonzeromin(img)
else:
pmin=img.min()
thresholds=pmin+firstThreshold+(img.max()-pmin-firstThreshold)//nrThresholds*numpy.arange(nrThresholds)
Ts=[majority_filter(img>T) for T in thresholds]
obj_count = 0
Ts.append(Ts[0]*0)
labeled0,N0=ndimage.label(Ts[0])
for T1 in Ts[1:]:
labeled1,N1=ndimage.label(T1)
for obj in xrange(N0):
binimg=(labeled0 == (obj+1))
objects1=(labeled1*binimg)
if not objects1.any() or ndimage.label(objects1)[1] == 1:
obj_count += 1
output[binimg]=obj_count
labeled0=labeled1
N0=N1
return output,obj_count
def remove_small_objects(ar, min_size=64, connectivity=1, in_place=False):
# Should use `issubdtype` for bool below, but there's a bug in numpy 1.7
if not (ar.dtype == bool or np.issubdtype(ar.dtype, np.integer)):
raise TypeError("Only bool or integer image types are supported. "
"Got %s." % ar.dtype)
if in_place:
out = ar
else:
out = ar.copy()
if min_size == 0: # shortcut for efficiency
return out
if out.dtype == bool:
selem = nd.generate_binary_structure(ar.ndim, connectivity)
ccs = np.zeros_like(ar, dtype=np.int32)
nd.label(ar, selem, output=ccs)
else:
ccs = out
try:
component_sizes = np.bincount(ccs.ravel())
except ValueError:
raise ValueError("Negative value labels are not supported. Try "
"relabeling the input with `scipy.ndimage.label` or "
"`skimage.morphology.label`.")
too_small = component_sizes < min_size
too_small_mask = too_small[ccs]
out[too_small_mask] = 0
return out
def erode_to_connected(img,d=3,shape=3,rel_size=0.5,min_size=15, single=False):
"""
erode to structure to specified relative size; safe version, which
won't collapse to a single pixel with connectivity constraints
"""
orig_size = np.count_nonzero(img)
new_size = orig_size
_,comp = ndimage.label(img)
if(orig_size <= 0 or comp > 1):
return img
prev_img = img.copy()
count = 0
# run for d times, but terminate early if the size (relative or
# absolute) is too small, or the image breaks into multiple pieces
while new_size > rel_size * orig_size \
and new_size > min_size \
and comp < 2 \
and count < d:
prev_img = img.copy()
img = erode(img,shape)
new_size = np.count_nonzero(img)
_,comp = ndimage.label(img)
count += 1
return prev_img
def remove_small_blobs(img, threshold=1, debug=True):
'''
Find blobs/clusters of same label. Only keep blobs with more than threshold elements.
This can be used for postprocessing.
:param img: 3D Image
:param threshold:
:return:
'''
# mask, number_of_blobs = ndimage.label(img, structure=np.ones((3, 3, 3))) #Also considers diagonal elements for determining if a element belongs to a blob
mask, number_of_blobs = ndimage.label(img)
print('Number of blobs before: ' + str(number_of_blobs))
counts = np.bincount(mask.flatten()) # number of pixels in each blob
if debug:
print(counts)
remove = counts <= threshold
remove_idx = np.nonzero(remove)[0] # somehow returns tupple with 1 value -> remove tupple, only keep value
for idx in remove_idx:
mask[mask == idx] = 0 # set blobs we remove to 0
mask[mask > 0] = 1 # set everything else to 1
if debug:
mask_after, number_of_blobs_after = ndimage.label(mask)
print('Number of blobs after: ' + str(number_of_blobs_after))
return mask
def threshold_and_clean_tomo_layer(reconstruction_fbp,recon_thresh, noise_obj_size,min_hole_size,edge_cleaning_iter=None):
binary_recon=reconstruction_fbp>recon_thresh
#hard codeed cleaning, grinding sausage...
binary_recon=img.morphology.binary_erosion(binary_recon,iterations=1)
binary_recon=img.morphology.binary_dilation(binary_recon,iterations=4)
labeled_img,num_labels=img.label(binary_recon)
print('Cleaning...')
print('Removing Noise...')
for ii in np.arange(1,num_labels):
obj1=np.where(labeled_img==ii)
if obj1[0].shape[0]<noise_obj_size:
binary_recon[obj1[0],obj1[1]]=0
labeled_img,num_labels=img.label(binary_recon!=1)
print('Closing Holes...')
for ii in np.arange(1,num_labels):
obj1=np.where(labeled_img==ii)
if obj1[0].shape[0]>=1 and obj1[0].shape[0]<min_hole_size:
binary_recon[obj1[0],obj1[1]]=1
if edge_cleaning_iter is not None:
binary_recon=img.morphology.binary_erosion(binary_recon,iterations=edge_cleaning_iter)
binary_recon=img.morphology.binary_dilation(binary_recon,iterations=edge_cleaning_iter)
return binary_recon
def threshold_segment(dna, threshold_method="otsu", smooth=None, median_size=5, min_obj_size=2500):
"""
labeled = threshold_method(dna, threshold_method='otsu',median_size=5,min_obj_size=2500)
Simple threshold-based segmentation
Params
------
* dna: either a pyslic.Image or a DNA image
* threshold_method: thresholding method to use. Can be either a function or
string which denotes the name of a function in pyslic.imageprocessing.thresholding (default: otsu)
* smooth: either None (no smoothing) or a sigma value for a gaussian blur (default: None)
* median_size: median filter size (default: 5). Set to None to skip filtering.
* min_obj_size: minimum object size (default: 2500)
"""
if type(dna) == Image:
with loadedimage(dna):
return threshold_segment(dna.get("dna"), threshold_method, smooth, median_size, min_obj_size)
if smooth is not None:
dna = ndimage.gaussian_filter(dna, smooth)
T = threshold(dna, threshold_method)
binimg = dna > T
if median_size is not None:
binimg = majority_filter(binimg, median_size)
L, N = ndimage.label(binimg)
if N == 0:
return L
sizes = numpy.array(ndimage.sum(binimg, L, numpy.arange(N + 1)))
for oid in numpy.where(sizes < min_obj_size)[0]:
L[L == oid] = 0
L, N = ndimage.label(L != 0)
return L
def get_stomata(max_proj_image, min_obj_size=200, max_obj_size=1000):
"""Performs image segmentation from a max_proj_image.
Disposes of objects in range min_obj_size to
max_obj_size
:param max_proj_image: the maximum projection image
:type max_proj_image: numpy.ndarray, uint16
:param min_obj_size: minimum size of object to keep
:type min_obj_size: int
:param max_obj_size: maximum size of object to keep
:type max_obj_size: int
:returns: list of [ [coordinates of kept objects - list of slice objects],
binary object image - numpy.ndarray,
labelled object image - numpy.ndarray
]
"""
# pore_margin = 10
# max_obj_size = 1000
# min_obj_size = 200
# for prop, value in segment_options:
# if prop == 'pore_margin':
# pore_margin = value
# if prop == 'max_obj_size':
# max_obj_size = value
# if prop == 'min_obj_size':
# min_obj_size = value
#
# print(pore_margin)
# print(max_obj_size)
# print(min_obj_size)
#rescale_min = 50
#rescale_max= 100
#rescaled = exposure.rescale_intensity(max_proj_image, in_range=(rescale_min,rescale_max))
rescaled = max_proj_image
seed = np.copy(rescaled)
seed[1:-1, 1:-1] = rescaled.max()
#mask = rescaled
#if gamma != None:
# rescaled = exposure.adjust_gamma(max_proj_image, gamma)
#filled = reconstruction(seed, mask, method='erosion')
closed = dilation(rescaled)
seed = np.copy(closed)
seed[1:-1, 1:-1] = closed.max()
mask = closed
filled = reconstruction(seed, mask, method='erosion')
label_objects, nb_labels = ndimage.label(filled)
sizes = np.bincount(label_objects.ravel())
mask_sizes = sizes
mask_sizes = (sizes > min_obj_size) & (sizes < max_obj_size)
#mask_sizes = (sizes > 200) & (sizes < 1000)
mask_sizes[0] = 0
big_objs = mask_sizes[label_objects]
stomata, _ = ndimage.label(big_objs)
obj_slices = ndimage.find_objects(stomata)
return [obj_slices, big_objs, stomata]
def findPlantsCanny(stackVar, stackSum, showImages=True):
edges = canny(stackVar)
fill_stack = ndimage.binary_fill_holes(edges)
label_objects, nb_labels = ndimage.label(fill_stack)
sizes = np.bincount(label_objects.ravel())
mask_sizes = sizes > 25
for label in range(len(mask_sizes)):
'''
Get rid of lines in addition to the straight size threshold.
'''
pts = np.where(label_objects == label)
xRange = (max(pts[0]) - min(pts[0]))
yRange = (max(pts[1]) - min(pts[1]))
areaCovered = float(len(pts[0])) / (xRange*yRange)
if (areaCovered < .33) or (xRange < 3) or (yRange < 3):
mask_sizes[label] = False
mask_sizes[0] = 0
plants_cleaned = mask_sizes[label_objects]
labeled_plants, numPlants = ndimage.label(plants_cleaned)
center = findCenters(labeled_plants, stackSum)
if showImages:
fig, axs = plt.subplots(1,3, figsize=(14,4), sharey=True)
axs[0].imshow(stackVar)
axs[1].imshow(stackVar, cmap=plt.cm.jet, interpolation='nearest') #@UndefinedVariable
axs[1].contour(plants_cleaned, [0.5], linewidths=1.2, colors='y')
axs[2].imshow(labeled_plants, cmap=plt.cm.spectral, interpolation='nearest') #@UndefinedVariable
axs[2].scatter(np.array(center.tolist())[:,1], np.array(center.tolist())[:,0],
color='grey')
for ax in axs: ax.axis('off')
fig.subplots_adjust(wspace=.01)
return labeled_plants, center
def create_paths(pathimage):
# shortest path through black then purple then cyan
arr = np.transpose(pygame.surfarray.array3d(pathimage), [1,0,2])
maxval = arr.max()
black = (((arr == 0).sum(axis=2)) == 3)
print "found %d black pixels"%(black.sum())
purple = ((arr[:,:,0] == maxval) & (arr[:,:,1] == 0) & (arr[:,:,2] == maxval))
print "found %d purple pixels"%(purple.sum())
cyan = ((arr[:,:,0] == 0) & (arr[:,:,1] == maxval) & (arr[:,:,2] == maxval))
print "found %d cyan pixels"%(cyan.sum())
white = (((arr == arr.max()).sum(axis=2)) == 3)
print "found %d white pixels"%(white.sum())
mask = black | purple | cyan | white
black_blobs, black_count = ndi.label(black)
purple_blobs, purple_count = ndi.label(purple)
cyan_blobs, cyan_count = ndi.label(cyan)
print black_count, purple_count, cyan_count
try:
black_centers, purple_distances, cyan_distances = cPickle.load(open("distances.pickle"))
except:
black_centers = ndi.center_of_mass(np.ones(black_blobs.shape), black_blobs, range(1, black_count + 1))
purple_distances = [masked_distance(purple_blobs == (b + 1), mask) for b in range(purple_count)]
cyan_distances = [masked_distance(cyan_blobs == (b + 1), mask) for b in range(cyan_count)]
cPickle.dump((black_centers, purple_distances, cyan_distances), open("distances.pickle", "w"))
return black_centers, [purple_distances, cyan_distances]
def skeleton(seg):
skel, dist = skmorph.medial_axis(seg, return_distance=True)
node, edge, leaf = (spim.label(g, np.ones((3, 3), bool))[0] for g in skel2graph(skel))
trim_edge = (edge != 0) & ~(skmorph.binary_dilation(node != 0, np.ones((3, 3), bool)) != 0)
trim_edge = spim.label(trim_edge, np.ones((3, 3), bool))[0]
leaf_edge_vals = skmorph.binary_dilation(leaf != 0, np.ones((3, 3), bool)) != 0
leaf_edge_vals = np.unique(trim_edge[leaf_edge_vals])
leaf_edge_vals = leaf_edge_vals[leaf_edge_vals > 0]
leaf_edge = leaf != 0
trim_edge = ndshm.fromndarray(trim_edge)
leaf_edge = ndshm.fromndarray(leaf_edge)
Parallel()(delayed(set_msk)(leaf_edge, trim_edge, l) for l in leaf_edge_vals)
trim_edge = np.copy(trim_edge)
leaf_edge = np.copy(leaf_edge)
leaf_edge[(skmorph.binary_dilation(leaf_edge, np.ones((3, 3), bool)) != 0) & (edge != 0)] = True
leaf_edge = spim.label(leaf_edge, np.ones((3, 3), bool))[0]
leaf_edge_node = skmorph.binary_dilation(leaf_edge != 0, np.ones((3, 3), bool)) != 0
leaf_edge_node = ((node != 0) & leaf_edge_node) | leaf_edge
leaf_edge_node = spim.label(leaf_edge_node, np.ones((3, 3), bool))[0]
cand_node = leaf_edge_node * (node != 0)
cand_node = cand_node.nonzero()
cand_node = np.transpose((leaf_edge_node[cand_node],) + cand_node + (2 * dist[cand_node],))
cand_leaf = leaf_edge_node * (leaf != 0)
cand_leaf = cand_leaf.nonzero()
cand_leaf = np.transpose((leaf_edge_node[cand_leaf],) + cand_leaf)
if len(cand_node) > 0 and len(cand_leaf) > 0:
cand_leaf = ndshm.fromndarray(cand_leaf)
cand_node = ndshm.fromndarray(cand_node)
pruned = Parallel()(delayed(prune_leaves)(cand_leaf, cand_node, j) for j in np.unique(cand_node[:, 0]))
cand_leaf = np.copy(cand_leaf)
cand_node = np.copy(cand_node)
pruned_ind = []
for p in pruned:
pruned_ind.extend(p)
pruned_ind = tuple(np.transpose(pruned_ind))
pruned = ~skel
pruned = ndshm.fromndarray(pruned)
leaf_edge = ndshm.fromndarray(leaf_edge)
Parallel()(delayed(set_msk)(pruned, leaf_edge, l) for l in np.unique(leaf_edge[pruned_ind]))
pruned = np.copy(pruned)
leaf_edge = np.copy(leaf_edge)
pruned = ~pruned
else:
pruned = skel
return pruned
def run(self, workspace):
objects = workspace.object_set.get_objects(self.objects_name.value)
assert isinstance(objects, cpo.Objects)
labels = objects.segmented
if self.relabel_option == OPTION_SPLIT:
output_labels, count = scind.label(labels > 0, np.ones((3, 3), bool))
else:
if self.unify_option == UNIFY_DISTANCE:
mask = labels > 0
if self.distance_threshold.value > 0:
#
# Take the distance transform of the reverse of the mask
# and figure out what points are less than 1/2 of the
# distance from an object.
#
d = scind.distance_transform_edt(~mask)
mask = d < self.distance_threshold.value / 2 + 1
output_labels, count = scind.label(mask, np.ones((3, 3), bool))
output_labels[labels == 0] = 0
if self.wants_image:
output_labels = self.filter_using_image(workspace, mask)
elif self.unify_option == UNIFY_PARENT:
parent_objects = workspace.object_set.get_objects(self.parent_object.value)
output_labels = parent_objects.segmented.copy()
output_labels[labels == 0] = 0
output_objects = cpo.Objects()
output_objects.segmented = output_labels
if objects.has_small_removed_segmented:
output_objects.small_removed_segmented = copy_labels(objects.small_removed_segmented, output_labels)
if objects.has_unedited_segmented:
output_objects.unedited_segmented = copy_labels(objects.unedited_segmented, output_labels)
output_objects.parent_image = objects.parent_image
workspace.object_set.add_objects(output_objects, self.output_objects_name.value)
measurements = workspace.measurements
add_object_count_measurements(measurements, self.output_objects_name.value, np.max(output_objects.segmented))
add_object_location_measurements(measurements, self.output_objects_name.value, output_objects.segmented)
#
# Relate the output objects to the input ones and record
# the relationship.
#
children_per_parent, parents_of_children = objects.relate_children(output_objects)
measurements.add_measurement(
self.objects_name.value, FF_CHILDREN_COUNT % self.output_objects_name.value, children_per_parent
)
measurements.add_measurement(
self.output_objects_name.value, FF_PARENT % self.objects_name.value, parents_of_children
)
if self.wants_outlines:
outlines = cellprofiler.cpmath.outline.outline(output_labels)
outline_image = cpi.Image(outlines.astype(bool))
workspace.image_set.add(self.outlines_name.value, outline_image)
if workspace.frame is not None:
workspace.display_data.orig_labels = objects.segmented
workspace.display_data.output_labels = output_objects.segmented
def find_peaks(file):
#read image data
f=pyfits.open(file)
img=f[0].data
#set NaN pixels (empty pixels) to zero
img[img != img]=0.0
img[img<0.]=0.0
if dim==4:
img=img[0,0,:,:] #gets rid of 3 and 4 dimensions, since FIRST fits files have four axis but only first two have data
T=ndimage.standard_deviation(img)
sourcelabels,num_sources=ndimage.label(img>T)
backgroundlabels,num_background=ndimage.label(img<T)
# define an 8-connected neighbourhood
neighborhood = generate_binary_structure(2,2)
fimg=img*sourcelabels
#apply the local maximum filter; all pixel of maximal value
#in their neighbourhood are set to 1
local_max=maximum_filter(fimg,footprint=neighborhood)==fimg
#In order to isolate the peaks we must remove the background from the mask.
#we create the mask of the background
background=img*backgroundlabels
#we must erode the background in order to
#successfully subtract it form local_max, otherwise a line will
#appear along the background border (artifact of the local maximum filter)
eroded_background=binary_erosion(background,structure=neighborhood,border_value=1)
#we obtain the final mask, containing only peaks,
#by removing the background from the local_max mask
detected_peaks=local_max-eroded_background
#contains some peaks not in source (background bright features), but code can remove these
#now need to find positions of these maximum
#label peaks
peaklabels,num_peaks=ndimage.measurements.label(detected_peaks)
#get peak positions
slices = ndimage.find_objects(peaklabels)
x, y = [], []
for dy,dx in slices:
x_center = (dx.start + dx.stop - 1)/2
x.append(x_center)
y_center = (dy.start + dy.stop - 1)/2
y.append(y_center)
peak_positions=zip(x,y)
#get peak values, in Jy/beam
peak_fluxes=[]
for coord in peak_positions:
peak_fluxes.append(img[coord[1],coord[0]])
peaks=zip(peak_positions,peak_fluxes)
#sort by peak_fluxes. Two brightest peaks will be the first two in the list
peaks=sorted(peaks,key=lambda l: l[1])
peaks.reverse()
return peaks,img,f
def test_connect_regions_with_grid():
lena = sp.lena()
mask = lena > 50
graph = grid_to_graph(*lena.shape, **{"mask": mask})
assert_equal(ndimage.label(mask)[1], cs_graph_components(graph)[0])
mask = lena > 150
graph = grid_to_graph(*lena.shape, **{"mask": mask, "dtype": None})
assert_equal(ndimage.label(mask)[1], cs_graph_components(graph)[0])
def split_up_binary_regions(seq_block, opening_param = 3, mshape = ((0,1,0),(1,1,1),(0,1,0)), min_size = 20):
# for each region:
# dilate, relabel, create stack with eroded single regions
# delete overlapping pixels, recombine, assigning unique labels
zdim, xdim, ydim = seq_block.shape
splitblock = np.zeros_like(seq_block)
for sidx in range(zdim):
num_labels = int(np.max(seq_block[sidx]))
if num_labels <= 1:
continue
max_label = num_labels
for lidx in range(num_labels):
comp = extract_region(seq_block[sidx], lidx+1)
if np.sum(comp) < min_size:
continue
component = ndi.binary_erosion(comp,structure = mshape,iterations = opening_param)
component, numnewcomponents = ndi.label(component)
if numnewcomponents == 1: # component is not split up by erosion
#component[component>0] = 1
#component = ndi.binary_dilation(component,structure = mshape,iterations = opening_param)
splitblock[sidx][comp > 0] = int(lidx+1)
else:
compstack = np.zeros((numnewcomponents,xdim,ydim))
for cidx in range(numnewcomponents):
compstack[cidx] = ndi.binary_dilation(extract_region(component, cidx+1),structure = mshape,iterations = opening_param)
compstack[cidx] = ndi.binary_dilation(compstack[cidx],structure = ((0,1,0),(1,1,1),(0,1,0)),iterations = 2)
overlapmask = np.sum(compstack, axis = 0)
comp[overlapmask > 1] = 0
comp, numnewcomponents2 = ndi.label(comp)
#if numnewcomponents != numnewcomponents2:
#print "Lost something on the way: C1 = " + str(numnewcomponents) + ", C2 = " + str(numnewcomponents2)
for cidx in range(numnewcomponents2):
component = extract_region(comp, cidx+1)
if np.sum(component) < min_size:
continue
#compstack[cidx] = compstack[cidx] * overlapmask
#if np.sum(compstack[cidx]) > 0:
max_label = max_label + 1
#compstack[cidx] = compstack[cidx] * max_label
splitblock[sidx] = splitblock[sidx] + component.astype(int) * int(max_label)
#splitblock[sidx] = splitblock[sidx] + np.sum(compstack,axis = 0).astype(int)
# relabel components to unique labels
all_labels = np.sort(np.unique(splitblock[sidx]))
dum = np.zeros_like(splitblock[sidx])
l_idx = 1
for label in all_labels:
if label >0:
dum[splitblock[sidx]==label] = l_idx
l_idx = l_idx + 1
return splitblock
def isolatefilaments(skel_img, size_threshold, pad_size=5):
'''
This function separates each filament, over a threshold of number of
pixels, into its own array with the same dimensions as the inputed image.
Parameters
----------
skel_img : numpy.ndarray
the resultant skeletons from the Medial Axis Transform
mask : numpy.ndarray
the binary mask from adaptive thresholding
size_threshold : int
sets the pixel size on the size of objects
Returns
-------
skelton_arrays : list
contains the individual arrays for each skeleton
mask : numpy.ndarray
Updated version of the mask where small objects have been eliminated
num : int
Number of filaments
corners : list
Contains the indices where each skeleton array was taken from
the original
'''
skeleton_arrays = []; pix_val = []; corners = []
labels,num = nd.label(skel_img,eight_con())
sums = nd.sum(skel_img,labels,range(num))
for n in range(num):
if sums[n]<size_threshold:
x,y = np.where(labels==n)
for i in range(len(x)):
skel_img[x[i],y[i]]=0
# Relabel after deleting short skeletons.
labels,num = nd.label(skel_img,eight_con())
# Split each skeleton into its own array.
for n in range(1,num+1):
x,y = np.where(labels==n)
# Make an array shaped to the skeletons size and padded on each edge
shapes = (x.max()-x.min()+2*pad_size, y.max()-y.min()+2*pad_size)
eachfil = np.zeros(shapes)
for i in range(len(x)):
eachfil[x[i]-x.min()+pad_size,y[i]-y.min()+pad_size] = 1
skeleton_arrays.append(eachfil)
# Keep the coordinates from the original image
lower = (x.min()-pad_size,y.min()-pad_size)
upper = (x.max()+pad_size,y.max()+pad_size)
corners.append([lower,upper])
return skeleton_arrays, num, corners
def test_connect_regions_with_grid():
lena = sp.misc.lena()
mask = lena > 50
graph = grid_to_graph(*lena.shape, mask=mask)
assert_equal(ndimage.label(mask)[1], cs_graph_components(graph)[0])
mask = lena > 150
graph = grid_to_graph(*lena.shape, mask=mask, dtype=None)
assert_equal(ndimage.label(mask)[1], cs_graph_components(graph)[0])
def test_connect_regions_with_grid():
lena = sp.lena()
mask = lena > 50
graph = grid_to_graph(*lena.shape, **{'mask' : mask})
nose.tools.assert_equal(ndimage.label(mask)[1],
cs_graph_components(graph)[0])
mask = lena > 150
graph = grid_to_graph(*lena.shape, **{'mask' : mask, 'dtype' : None})
nose.tools.assert_equal(ndimage.label(mask)[1],
cs_graph_components(graph)[0])
def plot_filled(y, ax=None, thresh=None, plot_thresh=True, color='k', lw=2, facecolor='0.8', two_tailed=False, thresh_color='k', autoset_ylim=True, label=None): ''' Plot a filled cluster. (This function should only be used through **spm1d.plot.plot_spmi**) ''' y = np.asarray(y, dtype=float) x = _getQ(None, y.size) ax = _gca(ax) if thresh==None: thresh = 0 x0,y0,ind0 = x.copy(), y.copy(), np.arange(y.size) ### threshold: if two_tailed: L,n = ndimage.label(np.abs(y)>thresh) else: L,n = ndimage.label(y>thresh) ### plot: ax.plot(x0, y0, color=color, lw=lw, label=label) ### create patches if needed: if n>0: polyg = [] for i in range(n): ind = ind0[L==i+1].tolist() x = x0[L==i+1].tolist() y = y0[L==i+1].tolist() csign = np.sign(y[0]) ### insert extra nodes for interpolation: x = [x[0]] + x + [x[-1]] y = [csign*thresh] + y + [csign*thresh] ### interpolate if necessary: if ind[0] != ind0[0]: dx = x0[ind[0]] - x0[ind[0]-1] dy = (csign*thresh - y0[ind[0]]) / (y0[ind[0]] - y0[ind[0]-1]) x[0] += dy*dx if ind[-1] != ind0[-1]: dx = x0[ind[-1]+1] - x0[ind[-1]] dy = (csign*thresh - y0[ind[-1]]) / (y0[ind[-1]+1] - y0[ind[-1]]) x[-1] += dy*dx polyg.append( Polygon(list(zip(x,y))) ) patches = PatchCollection(polyg, edgecolors=None) ax.add_collection(patches) pyplot.setp(patches, facecolor=facecolor, edgecolor=facecolor) #set axis limits: # pyplot.setp(ax, xlim=(x0.min(), x0.max()), ylim=(y0.min(), y0.max())) pyplot.setp(ax, xlim=(x0.min(), x0.max()) ) #plot threshold(s): if (thresh!=None) and plot_thresh: h = [ax.hlines(thresh, x0.min(), x0.max())] if two_tailed: h.append( ax.hlines(-thresh, x0.min(), x0.max()) ) pyplot.setp(h, color=thresh_color, lw=1, linestyle='--') if autoset_ylim: _set_ylim(ax)
def find_dead_ends_once(arr):
"""Called by find_dead_ends"""
Nx, Ny = arr.shape
# Surround whole grid with border of False values to avoid index errors
arr = np.append(arr, np.full((Nx, 1), False, dtype=bool), axis=1)
arr = np.append(arr, np.full((1, Ny + 1), False, dtype=np.bool), axis=0)
arr = np.insert(arr, 0, False, axis=1)
arr = np.insert(arr, 0, False, axis=0)
# Kernels used in convolution to identify possible dead ends
x_kernel = np.array([[1], [0], [1]])
y_kernel = np.array([[1, 0, 1]])
conv_x = convolve(arr, y_kernel, 'same')
conv_y = convolve(arr, x_kernel, 'same')
inds_x = np.logical_and(arr, conv_x == 0)
inds_x_labels, nxlabels = label(inds_x)
inds_y = np.logical_and(arr, conv_y == 0)
inds_y_labels, nylabels = label(inds_y)
# Find inds in first direction
for i in np.r_[1:nxlabels+1]:
args_i = np.argwhere(inds_x_labels == i)
y_inds = args_i[[0, -1], 0] + np.r_[-1, 1]
x_ind = args_i[0, 1]
if np.all(arr[y_inds, x_ind]):
# Not a dead end, water on both sides
inds_x_labels[inds_x_labels == i] = 0
inds_x = inds_x_labels > 0
# Same for second direction
for i in np.r_[1:nylabels+1]:
args_i = np.argwhere(inds_y_labels == i)
y_ind = args_i[0, 0]
x_inds = args_i[[0, -1], 1] + np.r_[-1, 1]
if np.all(arr[y_ind, x_inds]):
# Not a dead end, water on both sides
inds_y_labels[inds_y_labels == i] = 0
inds_y = inds_y_labels > 0
dead_end_inds = np.logical_or(inds_x, inds_y)
# Account for border of False values added at start
dead_end_inds = dead_end_inds[1:-1, 1:-1]
return dead_end_inds
def feature_vector_from_rgb(image, sample_size=None):
"""Compute a feature vector from the composite images.
The channels are assumed to be in the following order:
- Red: MCF-7
- Green: cytoplasm GFP
- Blue: DAPI/Hoechst
Parameters
----------
im : array, shape (M, N, 3)
The input image.
sample_size : int, optional
For features based on quantiles, sample this many objects
rather than computing full distribution. This can considerably
speed up computation with little cost to feature accuracy.
Returns
-------
fs : 1D array of float
The features of the image.
names : list of string
The feature names.
"""
all_fs, all_names = [], []
ims = np.rollaxis(image[..., :3], -1, 0) # toss out alpha chan if present
mcf, cells, nuclei = ims
prefixes = ['mcf', 'cells', 'nuclei']
for im, prefix in zip(ims, prefixes):
fs, names = features.intensity_object_features(im,
sample_size=sample_size)
names = [prefix + '-' + name for name in names]
all_fs.append(fs)
all_names.extend(names)
nuclei_mean = nd.label(nuclei > np.mean(nuclei))[0]
fs, names = features.nearest_neighbors(nuclei_mean)
all_fs.append(fs)
all_names.extend(['nuclei-' + name for name in names])
mcf_mean = nd.label(mcf)[0]
fs, names = features.fraction_positive(nuclei_mean, mcf_mean,
positive_name='mcf')
all_fs.append(fs)
all_names.extend(names)
cells_t_otsu = cells > threshold_otsu(cells)
cells_t_adapt = threshold_adaptive(cells, 51)
fs, names = features.nuclei_per_cell_histogram(nuclei_mean, cells_t_otsu)
all_fs.append(fs)
all_names.extend(['otsu-' + name for name in names])
fs, names = features.nuclei_per_cell_histogram(nuclei_mean, cells_t_adapt)
all_fs.append(fs)
all_names.extend(['adapt-' + name for name in names])
return np.concatenate(all_fs), all_names
def _process_B(B):
L,N = ndimage.label(~B)
bg = 0
bg_size = (L==bg).sum()
for i in xrange(1,N+1):
i_size = (L == i).sum()
if i_size > bg_size:
bg_size = i_size
bg = i
if i_size < _min_obj_size:
L[L==i] = 0
L[L==bg]=0
L,_ = ndimage.label(L!=0)
return L
def test_connect_regions_with_grid():
try:
face = sp.face(gray=True)
except AttributeError:
# Newer versions of scipy have face in misc
from scipy import misc
face = misc.face(gray=True)
mask = face > 50
graph = grid_to_graph(*face.shape, mask=mask)
assert_equal(ndimage.label(mask)[1], connected_components(graph)[0])
mask = face > 150
graph = grid_to_graph(*face.shape, mask=mask, dtype=None)
assert_equal(ndimage.label(mask)[1], connected_components(graph)[0])
def findNeuronsFromStDev(self,applyFilter=False,sigmax=3,sigmay=3,tRange=None,sizeLimit=40,distanceLimit=6):
stdFrame = np.zeros(np.shape(self.motionCorrectedMovie.mov[0]))
h,w = np.shape(stdFrame)
for r in range(h):
for c in range(w):
stdFrame[r][c] = np.std(self.motionCorrectedMovie.mov[:,r,c])
if applyFilter:
stdFrame = gaussian_filter(stdFrame,[sigmax,sigmay])
if tRange == None:
med = np.median(stdFrame)
stdStd = np.std(stdFrame)
tRange = [med + 5*stdStd, med + 4*stdStd, med + 3*stdStd, med + 2*stdStd, med + stdStd, med]
conmat = np.ones((3,3)) #np.array([[0,1,0],[1,1,1],[0,1,0]])
ntot = 0
for threshold in tRange:
stdFrameFree = stdFrame
for m in self.neuronMasks:
stdFrameFree = stdFrameFree * (1-(m>0))
mask = stdFrameFree*(stdFrameFree > threshold)
mask, n = label(mask > 0, conmat)
nRejected = 0
nPruned = 0
avgPixelsPruned = 0
for i in range(1,n+1):
iROI = (mask == i)
iSize = np.sum(iROI)
stdROI = iROI * stdFrameFree
[[rmax],[cmax]] = np.where(stdROI == np.max(stdROI))
r,c = np.where(iROI == 1)
for j in range(len(r)):
if ((r[j]-rmax)**2 + (c[j]-cmax)**2)**0.5 < distanceLimit:
iROI[r[j],c[j]] = 0
mask = mask * (1-iROI)
diff = np.sum(iROI)
iSizePruned = iSize - diff
if iSizePruned < sizeLimit:
iROI = (mask == i)
mask = mask * (1-iROI)
nRejected = nRejected + 1
elif diff > 0:
nPruned = nPruned + 1
avgPixelsPruned = avgPixelsPruned + diff
avgPixelsPruned = avgPixelsPruned / float(nPruned)
mask, nfinal = label(mask > 0, conmat)
self.neuronMasks.append(mask)
print '%i potential neurons found above threshold %f' % (n,threshold)
print '%i rejected after pruning for having size < %i' % (nRejected,sizeLimit)
print '%i kept neurons pruned, average %i pixels removed' % (nPruned,avgPixelsPruned)
ntot = ntot + n - nRejected
return ntot
def all_alive(board):
black_groups, black_count = nd.label(board == 1)
empty_adjacent_count = nd.filters.convolve((board == 0).astype(np.uint8),
np.array([[0, 1, 0], [1, 0, 1], [0, 1, 0]]),
mode='constant')
black_adjacent_max = nd.maximum(empty_adjacent_count, black_groups,
np.arange(1, black_count))
if np.any(black_adjacent_max == 0):
return False
white_groups, white_count = nd.label(board == 2)
white_adjacenet_max = nd.maximum(empty_adjacent_count, white_groups,
np.arange(1, white_count))
if np.any(white_adjacenet_max == 0):
return False
return True
def find_albino_features(self, T, im):
import scipy.ndimage as ndi
binarized = zeros_like(T)
binarized[T > self.albino_threshold] = True
(labels, nlabels) = ndi.label(binarized)
slices = ndi.find_objects(labels)
intensities = []
transform_means = []
if len(slices) < 2:
return (None, None)
for s in slices:
transform_means.append(mean(T[s]))
intensities.append(mean(im[s]))
sorted_transform_means = argsort(transform_means)
candidate1 = sorted_transform_means[-1]
candidate2 = sorted_transform_means[-2]
c1_center = array(ndi.center_of_mass(im, labels, candidate1 + 1))
c2_center = array(ndi.center_of_mass(im, labels, candidate2 + 1))
if intensities[candidate1] > intensities[candidate2]:
return (c2_center, c1_center)
else:
return (c1_center, c2_center)
def test_connect_regions():
lena = sp.lena()
for thr in (50, 150):
mask = lena > thr
graph = img_to_graph(lena, mask)
nose.tools.assert_equal(ndimage.label(mask)[1],
cs_graph_components(graph)[0])
def quantify_fibers2(self, args, vis_pix, thresholded_copix, im_co, rgb_pix): #label feature with number (2) vis_pix_matrix = vis_pix.reshape(im_co.size[1],im_co.size[0]) all_array, num_features = si.label(vis_pix_matrix,structure=[[1,1,1],[1,1,1],[1,1,1]]) co_fiber_sizes = [] for fiber_slice in si.find_objects(all_array): fiber_pix = (thresholded_copix[fiber_slice].flatten()) > 0 fiber_types = collections.Counter(all_array[fiber_slice].flatten()) max_type = None if len(fiber_types) > 2: max_count = 0 for ft in fiber_types: if ft > 0 and fiber_types[ft] > max_count: max_type = ft max_count = fiber_types[ft] if float(np.sum(fiber_pix))/float(fiber_pix.size) > .3: #if > 30% of the pixels are colocalized call entire fiber slow slow_fiber_size = 0 for i in xrange(fiber_slice[0].start,fiber_slice[0].stop): for j in xrange(fiber_slice[1].start,fiber_slice[1].stop): if vis_pix_matrix[i][j] == 1: if max_type: if all_array[i][j] == max_type: vis_pix_matrix[i][j] = 2 rgb_pix[i*im_co.size[0] + j] = (255, 255, 0) slow_fiber_size += 1 else: vis_pix_matrix[i][j] = 2 rgb_pix[i*im_co.size[0] + j] = (255, 255, 0) slow_fiber_size += 1 co_fiber_sizes.append(slow_fiber_size) vis_pix = vis_pix_matrix.flatten() return np.array(co_fiber_sizes)
def mark_lalo_anomoly(lat, lon):
"""mask pixels with abnormal values (0, etc.)
This is found on sentinelStack multiple swath lookup table file.
"""
# ignore pixels with zero value
zero_mask = np.multiply(lat != 0., lon != 0.)
# ignore anomaly non-zero values
# by get the most common data range (d_min, d_max) based on histogram
mask = np.array(zero_mask, np.bool_)
for data in [lat, lon]:
bin_value, bin_edge = np.histogram(data[mask], bins=10)
# if there is anomaly, histogram won't be evenly distributed
while np.max(bin_value) > np.sum(zero_mask) * 0.3:
# find the continous bins where the largest bin is --> normal data range
bin_value_thres = ut.median_abs_deviation_threshold(bin_value, cutoff=3)
bin_label = ndimage.label(bin_value > bin_value_thres)[0]
idx = np.where(bin_label == bin_label[np.argmax(bin_value)])[0]
# convert to min/max data value
bin_step = bin_edge[1] - bin_edge[0]
d_min = bin_edge[idx[0]] - bin_step / 2.
d_max = bin_edge[idx[-1]+1] + bin_step / 2.
mask *= np.multiply(data >= d_min, data <= d_max)
bin_value, bin_edge = np.histogram(data[mask], bins=10)
lat[mask == 0] = 90.
lon[mask == 0] = 0.
return lat, lon, mask
draw_line_endpoints(img, linesEndpoints)
lower = np.array(lower,
dtype="uint8") # brojevi su svelije nijanse sive
upper = np.array(upper, dtype="uint8")
mask = cv2.inRange(img, lower, upper)
# cv2.imshow("maska", mask)
img0 = 1.0 * mask
img0 = cv2.dilate(
img0, kernel
) #ako je broj prekriven sumom, dilatacija ce popuniti te praznine na samoj cifri
img0 = cv2.dilate(img0, kernel) #jedna dilatacija nije bila dovoljna
#cv2.imshow("dilat", img0)
labeled, nr_objects = ndimage.label(img0)
objects = ndimage.find_objects(labeled)
for i in range(nr_objects):
loc = objects[i]
(xc, yc) = (int((loc[1].stop + loc[1].start) / 2),
int((loc[0].stop + loc[0].start) / 2))
(dxc, dyc) = (int(loc[1].stop - loc[1].start),
int(loc[0].stop - loc[0].start))
if (dxc < 12 and dyc < 12):
continue
cv2.circle(img, (xc, yc), 16, (25, 25, 255), 1)
elem = {'center': (xc, yc), 'size': (dxc, dyc), 't': t}
lst = inRange(20, elem, elements)
def compute_metrics(pred_mask_binary, mask, metrics, img_name):
'''Read an image and return post-processed prediction according to a model.
Keyword arguments:
pred_mask_binary -- predicted mask
mask -- true mask
metrics -- pandas dataframe to store image-wise metrics
img_name -- name of the image to be processed
Return:
metrics -- pandas dataframe with image-wise metrics
'''
# extract predicted objects and counts
pred_label, pred_count = ndimage.label(pred_mask_binary)
pred_objs = ndimage.find_objects(pred_label)
# compute centers of predicted objects
pred_centers = []
for ob in pred_objs:
pred_centers.append(((int(
(ob[0].stop - ob[0].start) / 2) + ob[0].start), (int(
(ob[1].stop - ob[1].start) / 2) + ob[1].start)))
# extract target objects and counts
targ_label, targ_count = ndimage.label(mask)
targ_objs = ndimage.find_objects(targ_label)
# compute centers of target objects
targ_center = []
for ob in targ_objs:
targ_center.append(((int(
(ob[0].stop - ob[0].start) / 2) + ob[0].start), (int(
(ob[1].stop - ob[1].start) / 2) + ob[1].start)))
# associate matching objects, true positives
tp = 0
fp = 0
for pred_idx, pred_obj in enumerate(pred_objs):
min_dist = 30 # 1.5-cells distance is the maximum accepted
TP_flag = 0
for targ_idx, targ_obj in enumerate(targ_objs):
dist = hypot(pred_centers[pred_idx][0] - targ_center[targ_idx][0],
pred_centers[pred_idx][1] - targ_center[targ_idx][1])
if dist < min_dist:
TP_flag = 1
min_dist = dist
index = targ_idx
if TP_flag == 1:
tp += 1
TP_flag = 0
targ_center.pop(index)
targ_objs.pop(index)
# derive false negatives and false positives
fn = targ_count - tp
fp = pred_count - tp
# update metrics dataframe
metrics.loc[img_name] = [tp, fp, fn, targ_count, pred_count]
return (metrics)
def old_make_single_chipmask(fibparms,
meansep,
masktype='stellar',
exclude_top_and_bottom=False,
nx=4112,
ny=4096):
"""OLD ROUTINE, NOT CURRENTLY IN USE!!!"""
# some housekeeping...
while masktype.lower() not in [
"stellar", "sky2", "sky3", "lfc", "thxe", "background", "bg"
]:
print('ERROR: chipmask "type" not recognized!!!')
masktype = raw_input(
'Please enter "type" - valid options are ["object" / "sky2" / "sky3" / "LFC" / "ThXe" / "background" or "bg"]: '
)
# ny, nx = img.shape
chipmask = np.zeros((ny, nx))
# now actually make the "chipmask"
for pix in np.arange(nx):
if (pix + 1) % 100 == 0:
print('Pixel column ' + str(pix + 1) + '/4112')
for order in sorted(fibparms.keys()):
if masktype.lower() == 'stellar':
# for the object-fibres chipmask, take the middle between the last object fibre and first sky fibre at each end (ie the "gaps")
f_upper = 0.5 * (fibparms[order]['fibre_04']['mu_fit'] +
fibparms[order]['fibre_06']['mu_fit'])
f_lower = 0.5 * (fibparms[order]['fibre_24']['mu_fit'] +
fibparms[order]['fibre_26']['mu_fit'])
elif masktype.lower() == 'sky2':
# for the 2 sky fibres near the ThXe, we use the "gap" as the upper bound, and as the lower bound the trace of the lowermost sky fibre
# minus half the average fibre separation for this order and pixel location, ie halfway between the lowermost sky fibre and the ThXe fibre
f_upper = 0.5 * (fibparms[order]['fibre_24']['mu_fit'] +
fibparms[order]['fibre_26']['mu_fit'])
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower.coefficients[-1] -= 0.5 * meansep[order][pix]
elif masktype.lower() == 'sky3':
# for the 3 sky fibres near the LFC, we use the "gap" as the lower bound, and as the upper bound the trace of the uppermost sky fibre
# plus half the average fibre separation for this order and pixel location, ie halfway between the uppermost sky fibre and the LFC fibre
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper.coefficients[-1] += 0.5 * meansep[order][pix]
f_lower = 0.5 * (fibparms[order]['fibre_04']['mu_fit'] +
fibparms[order]['fibre_06']['mu_fit'])
elif masktype.lower() == 'lfc':
# for the LFC fibre, we assume as the lower bound the trace of the uppermost sky fibre plus half the average fibre separation for this order and pixel location,
# ie halfway between the uppermost sky fibre and the LFC fibre, and as the upper bound the trace of the uppermost sky fibre plus two times the average
# fibre separation for this order and pixel location
f_upper = 1 * fibparms[order]['fibre_02']['mu_fit']
f_upper.coefficients[-1] += 2. * meansep[order][pix]
f_lower = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower.coefficients[-1] += 0.5 * meansep[order][pix]
elif masktype.lower() == 'thxe':
# for the ThXe fibre, we assume as the upper bound the trace of the lowermost sky fibre minus half the average fibre separation for this order and pixel location,
# ie halfway between the lowermost sky fibre and the ThXe fibre, and as the lower bound the trace of the lowermost sky fibre minus two times the average
# fibre separation for this order and pixel location
f_upper = 1 * fibparms[order]['fibre_27']['mu_fit']
f_upper.coefficients[-1] -= 0.5 * meansep[order][pix]
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower.coefficients[-1] -= 2. * meansep[order][pix]
elif masktype.lower() in ['background', 'bg']:
# could either do sth like 1. - np.sum(chipmask_i), but can also just use the lower bound of ThXe and the upper bound of LFC
f_upper = 1 * fibparms[order]['fibre_02']['mu_fit']
f_upper.coefficients[-1] += 2. * meansep[order][pix]
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower.coefficients[-1] -= 2. * meansep[order][pix]
else:
print(
'ERROR: Nightmare! That should never happen -- must be an error in the Matrix...'
)
return
ymin = f_lower(pix)
ymax = f_upper(pix)
# these are the pixels that fall completely in the range
# NOTE THAT THE CO-ORDINATES ARE CENTRED ON THE PIXELS, HENCE THE 0.5s...
full_range = np.arange(
np.maximum(np.ceil(ymin + 0.5), 0),
np.minimum(np.floor(ymax - 0.5) + 1, ny - 1)).astype(int)
if len(full_range) > 0:
chipmask[full_range, pix] = 1.
# bottom edge pixel
if ymin > -0.5 and ymin < ny - 1 + 0.5:
qlow = np.ceil(ymin - 0.5) - ymin + 0.5
chipmask[np.floor(ymin + 0.5).astype(int), pix] = qlow
# top edge pixel
if ymax > -0.5 and ymax < ny - 1 + 0.5:
qtop = ymax - np.floor(ymax - 0.5) - 0.5
chipmask[np.ceil(ymax - 0.5).astype(int), pix] = qtop
# for the background we have to invert that mask and (optionally) exclude the top and bottom regions
# which still include fainter orders etc (same as in "extract_background")
if masktype.lower() == 'background':
chipmask = 1. - chipmask
if exclude_top_and_bottom:
print(
'WARNING: this fix works for the current Veloce CCD layout only!!!'
)
labelled_mask, nobj = label(chipmask)
# WARNING: this fix works for the current Veloce CCD layout only!!!
topleftnumber = labelled_mask[ny - 1, 0]
# toprightnumber = labelled_mask[ny-1,nx-1]
# bottomleftnumber = labelled_mask[0,0]
bottomrightnumber = labelled_mask[0, nx - 1]
chipmask[labelled_mask == topleftnumber] = False
# chipmask[labelled_mask == toprightnumber] = False
chipmask[labelled_mask == bottomrightnumber] = False
return chipmask
cmap=cm)
fig.savefig(
os.path.join(fig_dir,
'distribution_embedding_genotype_tbins=%d.pdf' % tbins))
#watershed it
from scipy import ndimage as ndi
from skimage.morphology import watershed
from skimage.feature import peak_local_max
local_maxi = peak_local_max(Y_xy, indices=False, footprint=np.ones((7, 7)))
local_maxi = peak_local_max(Y_xy, indices=False, footprint=np.ones((10, 10)))
markers = ndi.label(local_maxi)[0]
labels = watershed(-Y_xy, markers) #, mask=image)
#classify points
Y_idx = np.array(np.round(
(Y - [xmin, ymin]) / [xmax - xmin, ymax - ymin] * (npts - 1)),
dtype=int)
Y_idx[Y_idx < 0] = 0
Y_idx[Y_idx > npts - 1] = npts - 1
Y_class = labels[Y_idx[:, 0], Y_idx[:, 1]]
cmap = 'jet'
cmap = 'nipy_spectral'
cmap = 'hsv'
cmap = 'gist_ncar'
def Kristen_render(name_pattern,
group_id,
mCherry,
extranuclear_mCherry_pad,
GFP_orig,
mCherry_orig, output,
save=False, directory_to_save_to='verification'):
labels, _ = ndi.label(extranuclear_mCherry_pad)
unique_segmented_cells_labels = np.unique(labels)[1:]
mCherry_cutoff = np.zeros_like(mCherry)
qualifying_cell_label = []
qualifying_regression_stats = []
for cell_label in unique_segmented_cells_labels:
mCherry_2 = np.zeros_like(mCherry)
my_mask = labels == cell_label
average_apply_mask = np.mean(mCherry[my_mask])
intensity = np.sum(mCherry[my_mask])
binary_pad = np.zeros_like(mCherry)
binary_pad[my_mask] = 1
pixel = np.sum(binary_pad[my_mask])
if (average_apply_mask > .05 or intensity > 300) and pixel > 4000:
GFP_limited_to_cell_mask = cf._3d_stack_2d_filter(GFP_orig, my_mask)
mCherry_limited_to_cell_mask = cf._3d_stack_2d_filter(mCherry_orig, my_mask)
qualifying_3d_GFP = GFP_limited_to_cell_mask[mCherry_limited_to_cell_mask>50]
average_3d_GFP = np.mean(qualifying_3d_GFP)
median_3d_GFP = np.median(qualifying_3d_GFP)
std_3d_GFP = np.std(qualifying_3d_GFP)
sum_qualifying_GFP = np.sum(qualifying_3d_GFP)
nonqualifying_3d_GFP = GFP_limited_to_cell_mask[mCherry_limited_to_cell_mask<=50]
average_nonqualifying_3d_GFP = np.mean(nonqualifying_3d_GFP)
median_nonqualifying_3d_GFP = np.median(nonqualifying_3d_GFP)
std_nonqualifying_3d_GFP = np.std(nonqualifying_3d_GFP)
sum_nonqualifying_GFP = np.sum(nonqualifying_3d_GFP)
sum_total_GFP = sum_qualifying_GFP + sum_nonqualifying_GFP
percent_qualifying_over_total_GFP = sum_qualifying_GFP/sum_total_GFP
# report the percentage too or sums are sufficient?
GFP_orig_qualifying = cf._3d_stack_2d_filter(GFP_orig, my_mask)
mCherry_orig_qualifying = cf._3d_stack_2d_filter(mCherry_orig, my_mask)
mCherry_1d = mCherry_orig_qualifying[mCherry_orig_qualifying > 50]
GFP_1d = GFP_orig_qualifying[mCherry_orig_qualifying>50]
regression_results = stats.linregress(GFP_1d, mCherry_1d)
mCherry_2[my_mask] = mCherry[my_mask]
mCherry_cutoff[my_mask] = mCherry[my_mask]
qualifying_cell_label.append(cell_label)
qualifying_regression_stats.append((regression_results[0], regression_results[2], regression_results[3]))
name_pattern_split = name_pattern.split(' - ')
transfection_label = name_pattern_split[0]
cell_type = name_pattern_split[1]
exp_time = name_pattern_split[2]
image_number = name_pattern_split[4]
with open(output, 'ab') as output_file:
writer = csv_writer(output_file, delimiter='\t')
writer.writerow([transfection_label, cell_type, exp_time, image_number, cell_label, sum_qualifying_GFP, sum_total_GFP, average_3d_GFP, median_3d_GFP, std_3d_GFP, average_nonqualifying_3d_GFP, median_nonqualifying_3d_GFP, std_nonqualifying_3d_GFP, regression_results[0], regression_results[2], regression_results[3]])
plt.figure(figsize=(26.0, 15.0))
plt.title('Kristen\'s Data')
plt.suptitle(name_pattern)
main_ax = plt.subplot(221)
plt.subplot(221, sharex=main_ax, sharey=main_ax)
plt.title('mCherry Binary')
im = plt.imshow(extranuclear_mCherry_pad, interpolation='nearest', cmap = 'hot')
plt.colorbar(im)
plt.subplot(222, sharex=main_ax, sharey=main_ax)
plt.title('mCherry')
plt.imshow(mCherry, interpolation='nearest')
plt.contour(extranuclear_mCherry_pad, [0.5], colors='k')
plt.subplot(223)
dplt.better2D_desisty_plot(GFP_1d, mCherry_1d)
plt.title('mCherry Intensity as a Function of GFP Voxel')
plt.xlabel('GFP Voxel')
plt.ylabel('mCherry Intensity')
plt.subplot(224, sharex=main_ax, sharey=main_ax)
plt.title('mCherry-cutoff applied')
plt.imshow(mCherry_2, interpolation='nearest')
if not save:
plt.show()
else:
name_puck = directory_to_save_to + '/' + 'Kristen-' + name_pattern+ '_cell' + str(cell_label)+ '.png'
plt.savefig(name_puck)
plt.close()
plt.figure(figsize=(26.0, 15.0))
main_ax = plt.subplot(121)
plt.subplot(121, sharex=main_ax, sharey=main_ax)
plt.suptitle('mCherry Before and After Qualifying Cell Cutoff is Applied')
plt.title('mCherry')
im = plt.imshow(mCherry, interpolation='nearest')
plt.colorbar(im)
plt.subplot(122, sharex=main_ax, sharey=main_ax)
plt.title('mCherry')
plt.imshow(mCherry_cutoff, interpolation='nearest')
if not save:
plt.show()
else:
name_puck = directory_to_save_to + '/' + 'Kristen-' + name_pattern + 'cutoff_app' + '.png'
plt.savefig(name_puck)
plt.close()
return qualifying_regression_stats
def generate_saliency(self, input, target):
#self.model.zero_grad()
output = self.model(input)
#index: 0 layer: HP
# index: 1 layer: Ship
# index: 2 layer: Small Towers
# index: 3 layer: Big Towers
# index: 4 layer: Small Cities
# index: 5 layer: Big Cities
# index: 6 layer: Friend
# index: 7 layer: Enemy
#return (input.grad.clone()[0] * input)
input2 = input.cpu().clone()
image = np.zeros((40, 40))
input2 = input2.view(40, 40, 8)
#logical or of the input images to get the original image
#only do or over small big cities and towers to get proper coordinates of cities and towers
#otherwise it will include enemy ship too if enemy channel is included which will cause object to
#be merged with tower or city object
for i in range(2,6):
if i!= 0:
image = np.logical_or(image, input2[:, :, i].numpy())*1
#get the number of objects in the image
labeled_array, num_objects = label(image)
indices = []
for i in range(num_objects):
indices.append(np.argwhere(labeled_array == i+1))
#special stuff for ship channel
ship_image = input2[:, :, 1].clone().numpy()
ship_image[19][20] = 0
ship_image[20][20] = 0
ship_image[21][20] = 0
ship_array, ships = label(ship_image)
ship_indices = []
for i in range(ships):
ship_indices.append(np.argwhere(ship_array == i+1))
#self.generate_file(ship_array, 'mod_ship_array')
hp_array, hp = label(input2[:, :, 0].numpy())
hp_indices = []
for i in range(hp):
hp_indices.append(np.argwhere(hp_array == i+1))
values = torch.zeros(40*40*5)
values = values.view(40, 40, 5)
input2 = input.clone()
input2 = input2.view(40, 40, 8)
# index: 0 layer: HP
# index: 1 layer: Ship
# index: 2 layer: Small Towers
# index: 3 layer: Big Towers
# index: 4 layer: Small Cities
# index: 5 layer: Big Cities
# index: 6 layer: Friend
# index: 7 layer: Enemy
#output layers:
#0 HP
#1 agent
#2 size
#3 type
# friend/enemy
#here i refers to the output salient layers
#print('num_objects', num_objects)
for i in range(5):
if i==0:# output HP
for j in range(hp):
for k in range(hp_indices[j].shape[0]):
x = hp_indices[j][k][0]
y = hp_indices[j][k][1]
temp = 0.3*input2[:, :, 0][x][y]
input2[:, :, 0][x][y] += temp
perturbed_output = self.model(input2.view(1, 12800))
gradient = (perturbed_output - output)/temp
input2 = input.clone()
input2 = input2.view(40, 40, 8)
for k in range(hp_indices[j].shape[0]):
x = hp_indices[j][k][0]
y = hp_indices[j][k][1]
values[:, :, 0][x][y] = gradient[:, target]
elif i==1:#output agent
for j in range(ships):
for k in range(ship_indices[j].shape[0]):
x = ship_indices[j][k][0]
y = ship_indices[j][k][1]
if input2[:, :, 1][x][y] == 1:
input2[:, :, 1][x][y] = 0
perturbed_output = self.model(input2.view(1, 12800))
gradient = (perturbed_output - output)
input2 = input.clone()
input2 = input2.view(40, 40, 8)
for k in range(ship_indices[j].shape[0]):
x = ship_indices[j][k][0]
y = ship_indices[j][k][1]
values[:, :, 1][x][y] = gradient[:, target]
elif i==2: #output size
#print('in i== 2')
for l in range(2, 6):
#print('layer: ',l)
for j in range(num_objects):
# print('object: ',j)
s = 0
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
# print('x: '+str(x)+' y: '+str(y))
# print('Value of input: '+str(input2[:, :, i][x][y]))
# print(input2[:, :, l][x][y])
if l == 2 or l==4: #small tower/city
if input2[:, :, l][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l+1][x][y] = 1
else: #big tower/city
if input2[:, :, l ][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l-1][x][y] = 1
perturbed_output = self.model(input2.view(1, 12800))
gradient = (perturbed_output - output)
#print(saliency)
input2 = input.clone()
input2 = input2.view(40, 40, 8)
if s==1:
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
values[:, :, 2][x][y] = gradient[:, target]
#print(saliency[0][target])
elif i==3: #output type
for l in range(2, 6):
for j in range(num_objects):
s = 0
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
# print('x: '+str(x)+' y: '+str(y))
# print('Value of input: '+str(input2[:, :, i][x][y]))
if l == 2 or l == 3: #small tower/city
if input2[:, :, l][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l+2][x][y] = 1
else: #big tower/city
if input2[:, :, l ][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l-2][x][y] = 1
perturbed_output = self.model(input2.view(1, 12800))
gradient = (perturbed_output - output)
#print(saliency)
input2 = input.clone()
input2 = input2.view(40, 40, 8)
if s==1:
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
values[:, :, 3][x][y] = gradient[:, target]
else:# output frenemy
for l in range(6, 8):
for j in range(num_objects):
s = 0
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
if l == 6:
if input2[:, :, l][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l+1][x][y] = 1
else:
if input2[:, :, l][x][y] == 1:
s = 1
input2[:, :, l][x][y] = 0
input2[:, :, l-1][x][y] = 1
perturbed_output = self.model(input2.view(1, 12800))
gradient = (perturbed_output - output)
#print(s)
input2 = input.clone()
input2 = input2.view(40, 40, 8)
if s==1:
for k in range(indices[j].shape[0]):
x = indices[j][k][0]
y = indices[j][k][1]
values[:, :, 4][x][y] = gradient[:, target]
#for l in range(6):
# torchvision.utils.save_image(saliency[:, :, l], "Image perturbed: "+str(i) + "/" + "object perturbed: "+str(j)+ "/" + str(l) + ".png", normalize=True)
return (values.view(1, 40*40*5))
"""
Display a labels layer above of an image layer using the add_labels and
add_image APIs
"""
from skimage import data
from scipy import ndimage as ndi
from napari_animation import Animation
import napari
blobs = data.binary_blobs(length=128, volume_fraction=0.1, n_dim=3)
viewer = napari.view_image(blobs.astype(float), name='blobs')
labeled = ndi.label(blobs)[0]
viewer.add_labels(labeled, name='blob ID')
animation = Animation(viewer)
viewer.update_console({'animation': animation})
animation.capture_keyframe()
viewer.camera.zoom = 0.2
animation.capture_keyframe()
viewer.camera.zoom = 10.0
viewer.camera.center = (0, 40.0, 10.0)
animation.capture_keyframe()
viewer.dims.current_step = (60, 0, 0)
animation.capture_keyframe(steps=60)
viewer.dims.current_step = (0, 0, 0)
animation.capture_keyframe(steps=60)
viewer.reset_view()
animation.capture_keyframe()
def compute_metrics2D(bin_im, dist_trsf_im, AP_pos=None, vs=None):
"""
Given an isotropic 2D binary image `bin_im`, a distance transform image
`dist_trsf_im` of `bin_im`, a 2D position and a voxel size `vs`,
computes the length, the width, the aspect ratio, the solidity,
the sphericity, the volume and the surface of the binary image.
Notes: - `bin_im` has to be isotropic otherwise the distances won't be
computed correctly
- `bin_im` and `dist_trsf_im` must share the same shape
Parameters
----------
bin_im : array_like
A n*m*l binary array with one single connected component
dist_trsf_im : array_like
A distance transformation of the bin_im array
AP_pos : ((float, float), (float, float)) optional
x, y position of the anterior part of the Neural Tube
vs : float optional (default 1.)
The size of the isotropic voxel in order to get measurements
in physical units
Returns
-------
length : float
The length of the masked object in the binary image
width_spl : scipy.interpolate.InterpolatedUnivariateSpline
The interpolated width along the masked object
in the binary image
width_median : float
Median of the width along the masked object in the binary image
AR : float
The aspect ratio of the masked object in the binary image
`length/median(width)`
solidity : float
The solidity of the masked object in the binary image
Ratio of pixels in the region to pixels of the convex hull image.
surface :
The surface of the masked object in the binary image
Number of voxels in the region
perimeter :
The perimeter of the masked object in the binary image
circularity :
The circularity of the masked object in the binary image
4*pi*(`surface`/(`perimeter`**2))
"""
# Extract the largest connected component
label_im = nd.label(bin_im)[0]
labels = np.unique(label_im)
labels = labels[labels != 0]
surfaces = nd.sum(np.ones_like(label_im), index=labels, labels=label_im)
final_cc = labels[np.argmax(surfaces)]
bin_im = (label_im == final_cc).astype(np.uint8)
# Compute the surface, perimeter, circularity and solidity
if vs is None:
vs = 1.
props = measure.regionprops(bin_im.astype(np.uint8))[0]
surface = props.area * vs**2
perimeter = props.perimeter * vs
circularity = 4 * np.pi * (surface / perimeter**2)
solidity = props.solidity
# Skeletonize the mask and retrieve the coordinates of the skeleton
skel_im = morphology.skeletonize(bin_im).astype(np.uint8)
pos_arr = np.argwhere(skel_im)
pos = dict(zip(range(len(pos_arr)), pos_arr))
nodes = set(pos)
neighb = {}
to_treat = set([min(nodes)])
done = set()
# Builds the tree of the skeleton
# The nodes are the coordinates
# Two nodes are linked iff they are 4-connected
while 0 < len(to_treat):
curr = to_treat.pop()
done.add(curr)
dist = np.abs(pos_arr[curr] - pos_arr)
N = set(np.where(np.max(dist, axis=1) == 1)[0])
for ni in N:
neighb.setdefault(curr, []).append(ni)
to_treat.update(N.difference(done))
# Finds the leaves of the tree
extremities = [k for k, v in neighb.items() if len(v) == 1]
D_out = {}
# For each leaf, finds the most distant leaf
# Using Dijkstra algorithm
with Pool() as pool:
mapping = [(nodes, neighb, e) for e in extremities]
out = pool.map(Dijkstra, mapping)
pool.terminate()
pool.close()
D_out = dict(zip(extremities, out))
# Finds the pair (e1, e2) of most distant leaves
e1 = max(D_out, key=lambda x: D_out.get(x)[1])
e2 = D_out[e1][0]
prev = D_out[e1][2]
curr = e2
# Retrieve and smooth the longest path of the skeleton tree
skel_im[skel_im != 0] = 1
path = []
while curr in prev:
path += [pos[curr]]
curr = prev[curr]
X, Y = zip(*path)
X_smoothed = np.round(nd.filters.gaussian_filter1d(X, sigma=2)).astype(
np.uint16)
Y_smoothed = np.round(nd.filters.gaussian_filter1d(Y, sigma=2)).astype(
np.uint16)
for x, y in zip(X_smoothed, Y_smoothed):
skel_im[tuple([x, y])] = 2
# Build the graph containing the longest path of the skeleton tree
pos_arr = np.argwhere(skel_im == 2)
pos = dict(zip(range(len(pos_arr)), pos_arr))
nodes = set(pos)
neighb = {}
to_treat = set([min(nodes)])
done = set()
while 0 < len(to_treat):
curr = to_treat.pop()
done.add(curr)
dist = np.abs(pos_arr[curr] - pos_arr)
N = set(np.where(np.max(dist, axis=1) == 1)[0])
for ni in N:
neighb.setdefault(curr, []).append(ni)
to_treat.update(N.difference(done))
# Retrieve the x, y, z coordinates of the longest path
first = list(neighb.keys())[0]
last, b, prev = Dijkstra((nodes, neighb, first))
last, b, prev = Dijkstra((nodes, neighb, last))
current = last
ordered_pos = [pos[current]]
while prev.get(current, -1) != -1:
current = prev[current]
ordered_pos += [pos[current]]
# Gets the closest points of the skeleton to
# the manually informed posterior and anterior positions
if AP_pos is not None:
A_pos, P_pos = AP_pos
A_pos = np.array(A_pos)
P_pos = np.array(P_pos)
dist_to_A = np.linalg.norm(ordered_pos - A_pos, axis=1)
A_pos = np.argmin(dist_to_A)
dist_to_P = np.linalg.norm(ordered_pos - P_pos, axis=1)
P_pos = np.argmin(dist_to_P)
# Crop and reorder (if necessary) the skeleton
if A_pos < P_pos:
ordered_pos = ordered_pos[A_pos:P_pos + 1]
ordered_pos = ordered_pos[::-1]
P_pos, A_pos = A_pos, P_pos
else:
ordered_pos = ordered_pos[P_pos:A_pos + 1]
# Computes, smooth and interpolate the width along the longest path
dist_trsf_im *= vs
x, y = zip(*ordered_pos)
width = dist_trsf_im[(x, y)].flatten()
width = nd.filters.gaussian_filter1d(width.astype(np.float), sigma=4)
X = np.linspace(0, 1, len(width))
width_spl = InterpolatedUnivariateSpline(X, width)
# Computes the length of the longest path in physical units (given by vs)
tmp = np.array(list(zip(x, y))) * vs
length = np.sum(np.linalg.norm(tmp[:-1] - tmp[1:], axis=1))
# Computes the Aspect Ration as the length over the median width
AR = length / np.median(width)
return (length, width_spl, np.median(width), AR, solidity, surface,
perimeter, circularity)
def Fragmenter():
tmpOb = Config.Load(
frgSpec,
pth.expanduser(
'~/korenbergNAS/3D_database/Working/configuration_files/SidescapeRelateBlockface/M{0}/section_{1}/section_{1}_frag0.yaml'
.format(secOb.mkyNum, secOb.secNum)))
dictBuild = {}
#Load in the whole image so that the fragment can cropped out
ssiSrc, bfiSrc, ssiMsk, bfiMsk = Loader(tmpOb, ca.MEM_HOST)
#Because some of the functions only woth with gray images
bfiGry = ca.Image3D(bfiSrc.grid(), bfiSrc.memType())
ca.Copy(bfiGry, bfiSrc, 1)
lblSsi, _ = ndimage.label(np.squeeze(ssiMsk.asnp()) > 0)
lblBfi, _ = ndimage.label(np.squeeze(bfiMsk.asnp()) > 0)
seedPt = np.squeeze(pp.LandmarkPicker([lblBfi, lblSsi]))
subMskBfi = common.ImFromNPArr(lblBfi == lblBfi[seedPt[0, 0],
seedPt[0,
1]].astype('int8'),
sp=bfiSrc.spacing(),
orig=bfiSrc.origin())
subMskSsi = common.ImFromNPArr(lblSsi == lblSsi[seedPt[1, 0],
seedPt[1,
1]].astype('int8'),
sp=ssiSrc.spacing(),
orig=ssiSrc.origin())
bfiGry *= subMskBfi
bfiSrc *= subMskBfi
ssiSrc *= subMskSsi
#Pick points that are the bounding box of the desired subvolume
corners = np.array(
pp.LandmarkPicker(
[np.squeeze(bfiGry.asnp()),
np.squeeze(ssiSrc.asnp())]))
bfiCds = corners[:, 0]
ssiCds = corners[:, 1]
#Extract the region from the source images
bfiRgn = cc.SubVol(bfiSrc,
xrng=[bfiCds[0, 0], bfiCds[1, 0]],
yrng=[bfiCds[0, 1], bfiCds[1, 1]])
ssiRgn = cc.SubVol(ssiSrc,
xrng=[ssiCds[0, 0], ssiCds[1, 0]],
yrng=[ssiCds[0, 1], ssiCds[1, 1]])
#Extract the region from the mask images
rgnMskSsi = cc.SubVol(subMskSsi,
xrng=[ssiCds[0, 0], ssiCds[1, 0]],
yrng=[ssiCds[0, 1], ssiCds[1, 1]])
rgnMskBfi = cc.SubVol(subMskBfi,
xrng=[bfiCds[0, 0], bfiCds[1, 0]],
yrng=[bfiCds[0, 1], bfiCds[1, 1]])
dictBuild['rgnBfi'] = np.divide(
bfiCds, np.array(bfiSrc.size().tolist()[0:2], 'float')).tolist()
dictBuild['rgnSsi'] = np.divide(
ssiCds, np.array(ssiSrc.size().tolist()[0:2], 'float')).tolist()
#Check the output directory for the source files of the fragment
if not pth.exists(
pth.expanduser(secOb.ssiSrcPath + 'frag{0}'.format(frgNum))):
os.mkdir(pth.expanduser(secOb.ssiSrcPath + 'frag{0}'.format(frgNum)))
if not pth.exists(
pth.expanduser(secOb.bfiSrcPath + 'frag{0}'.format(frgNum))):
os.mkdir(pth.expanduser(secOb.bfiSrcPath + 'frag{0}'.format(frgNum)))
#Check the output directory for the mask files of the fragment
if not pth.exists(
pth.expanduser(secOb.ssiMskPath + 'frag{0}'.format(frgNum))):
os.mkdir(pth.expanduser(secOb.ssiMskPath + 'frag{0}'.format(frgNum)))
if not pth.exists(
pth.expanduser(secOb.bfiMskPath + 'frag{0}'.format(frgNum))):
os.mkdir(pth.expanduser(secOb.bfiMskPath + 'frag{0}'.format(frgNum)))
dictBuild[
'ssiSrcName'] = 'frag{0}/M{1}_01_ssi_section_{2}_frag1.tif'.format(
frgNum, secOb.mkyNum, secOb.secNum)
dictBuild[
'bfiSrcName'] = 'frag{0}/M{1}_01_bfi_section_{2}_frag1.mha'.format(
frgNum, secOb.mkyNum, secOb.secNum)
dictBuild[
'ssiMskName'] = 'frag{0}/M{1}_01_ssi_section_{2}_frag1_mask.tif'.format(
frgNum, secOb.mkyNum, secOb.secNum)
dictBuild[
'bfiMskName'] = 'frag{0}/M{1}_01_bfi_section_{2}_frag1_mask.tif'.format(
frgNum, secOb.mkyNum, secOb.secNum)
#Write out the masked and cropped images so that they can be loaded from the YAML file
#The BFI region needs to be saved as color and mha format so that the grid information is carried over.
common.SaveITKImage(
ssiRgn, pth.expanduser(secOb.ssiSrcPath + dictBuild['ssiSrcName']))
cc.WriteColorMHA(
bfiRgn, pth.expanduser(secOb.bfiSrcPath + dictBuild['bfiSrcName']))
common.SaveITKImage(
rgnMskSsi, pth.expanduser(secOb.ssiMskPath + dictBuild['ssiMskName']))
common.SaveITKImage(
rgnMskBfi, pth.expanduser(secOb.bfiMskPath + dictBuild['bfiMskName']))
frgOb = Config.MkConfig(dictBuild, frgSpec)
updateFragOb(frgOb)
return None
# We can solve this by applying some smoothing to the image data.
# Here we apply a 2D Gaussian smoothing function to the data.
data2 = ndimage.gaussian_filter(aiamap.data * ~mask, 14)
##############################################################################
# The issue with the filtering is that it create pixels where the values are
# small (<100), so when we go on later to label this array,
# we get one large region which encompasses the entire array.
# If you want to see, just remove this line.
data2[data2 < 100] = 0
##############################################################################
# Now we will make a second SunPy map with this smoothed data.
aiamap2 = sunpy.map.Map(data2, aiamap.meta)
##############################################################################
# The function `scipy.ndimage.label` counts the number of contiguous regions
# in an image.
labels, n = ndimage.label(aiamap2.data)
##############################################################################
# Finally, we plot the smoothed bright image data, along with the estimate of
# the number of distinct regions. We can see that approximately 6 distinct hot
# regions are present above the 10% of the maximum level.
plt.figure()
ax = plt.subplot(projection=aiamap)
aiamap.plot()
plt.contour(labels)
plt.figtext(0.3, 0.2, f'Number of regions = {n}', color='white')
plt.show()
def make_single_chipmask(fibparms,
meansep,
masktype='stellar',
exclude_top_and_bottom=True,
nx=4112,
ny=4096,
use_lfc=False,
debug_level=0,
timit=False):
if timit:
start_time = time.time()
# some housekeeping...
while masktype.lower() not in [
"stellar", "sky2", "sky3", "lfc", "thxe", "background", "bg"
]:
print('ERROR: chipmask "type" not recognized!!!')
masktype = raw_input(
'Please enter "type" - valid options are ["object" / "sky2" / "sky3" / "LFC" / "ThXe" / "background" or "bg"]: '
)
# ny, nx = img.shape
chipmask = np.zeros((ny, nx))
xx = np.arange(nx)
yy = np.arange(ny)
XX, YY = np.meshgrid(xx, yy)
# now identify regions of the chip
for order in sorted(fibparms.keys()):
if debug_level >= 1:
print('Checking ' + order)
if masktype.lower() == 'stellar':
# for the object-fibres chipmask, take the middle between the last object fibre and first sky fibre at each end (ie the "gaps")
f_upper = 0.5 * (fibparms[order]['fibre_04']['mu_fit'] +
fibparms[order]['fibre_06']['mu_fit'])
f_lower = 0.5 * (fibparms[order]['fibre_24']['mu_fit'] +
fibparms[order]['fibre_26']['mu_fit'])
elif masktype.lower() == 'sky2':
# for the 2 sky fibres near the ThXe, we use the "gap" as the upper bound, and as the lower bound either (i) the midpoint between the lowest sky2 fibre and
# the simThXe fibre, or (ii) the trace of the lowermost sky fibre minus half the average fibre separation for this order and pixel location
f_upper = 0.5 * (fibparms[order]['fibre_24']['mu_fit'] +
fibparms[order]['fibre_26']['mu_fit'])
try:
f_lower = 0.5 * (fibparms[order]['fibre_27']['mu_fit'] +
fibparms[order]['fibre_28']['mu_fit'])
except:
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 0.5 * meansep[order]
elif masktype.lower() == 'sky3':
# for the 3 sky fibres near the LFC, we use the "gap" as the lower bound, and as the upper bound either (i) the midpoint between the uppermost sky3 fibre and
# the LFC fibre, or (ii) the trace of the uppermost sky fibre plus half the average fibre separation for this order and pixel location
if use_lfc:
try:
f_upper = 0.5 * (fibparms[order]['fibre_02']['mu_fit'] +
fibparms[order]['fibre_01']['mu_fit'])
except:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 0.5 * meansep[order]
else:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 0.5 * meansep[order]
f_lower = 0.5 * (fibparms[order]['fibre_04']['mu_fit'] +
fibparms[order]['fibre_06']['mu_fit'])
elif masktype.lower() == 'lfc':
# for the LFC fibre, we assume as the lower bound either (i) the midpoint between the uppermost sky3 fibre and the LFC fibre, or (ii) the trace of the uppermost
# sky fibre plus half the average fibre separation for this order and pixel location; as the upper bound either (i) the trace of the LFC fibre plus half the average
# fibre separation for this order and pixel location, or (ii) the trace of the uppermost sky3 fibre plus one and a half times the average fibre separation for this
# order and pixel location
if use_lfc:
try:
f_upper = 1 * fibparms[order]['fibre_01'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 0.5 * meansep[order]
f_lower = 0.5 * (fibparms[order]['fibre_01']['mu_fit'] +
fibparms[order]['fibre_02']['mu_fit'])
except:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 1.5 * meansep[order]
f_lower = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower += 0.5 * meansep[order]
else:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 1.5 * meansep[order]
f_lower = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower += 0.5 * meansep[order]
elif masktype.lower() == 'thxe':
# for the simThXe fibre, we assume as the lower bound either (i) the trace of the simThXe fibre minus half the average fibre separation for this order and pixel
# location, or (ii) the trace of the lowermost sky2 fibre minus one and a half times the average fibre separation for this order and pixel location; as the upper
# bound either (i) the midpoint between the lowermost sky2 fibre and the simThXe fibre, or (ii) the trace of the lowermost sky2 fibre minus one a half times the
# average fibre separation for this order and pixel location
try:
f_upper = 0.5 * (fibparms[order]['fibre_27']['mu_fit'] +
fibparms[order]['fibre_28']['mu_fit'])
f_lower = 1 * fibparms[order]['fibre_28'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 0.5 * meansep[order]
except:
f_upper = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper -= 0.5 * meansep[order]
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 1.5 * meansep[order]
elif masktype.lower() in ['background', 'bg']:
# could either do sth like 1. - np.sum(chipmask_i), but we can also just use the lower bound of ThXe and the upper bound of LFC
# identify what is NOT background, and later "invert" that
if use_lfc:
try:
f_upper = 1 * fibparms[order]['fibre_01'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 1. * meansep[
order] # use whole meansep here to avoid contamination from the calib sources a bit more
f_lower = 1 * fibparms[order]['fibre_28'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 1. * meansep[
order] # use whole meansep here to avoid contamination from the calib sources a bit more
except:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 2. * meansep[
order] # use 2 here to avoid contamination from the calib sources a bit more
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 2. * meansep[
order] # use 2 here to avoid contamination from the calib sources a bit more
else:
f_upper = 1 * fibparms[order]['fibre_02'][
'mu_fit'] # the multiplication with one acts like a copy
f_upper += 2. * meansep[
order] # use 2 here to avoid contamination from the calib sources a bit more
try:
f_lower = 1 * fibparms[order]['fibre_28'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 1. * meansep[order]
except:
f_lower = 1 * fibparms[order]['fibre_27'][
'mu_fit'] # the multiplication with one acts like a copy
f_lower -= 2. * meansep[
order] # use 2 here to avoid contamination from the calib sources a bit more
# get indices of the pixels that fall into the respective regions
order_stripe = (YY < f_upper) & (YY > f_lower)
chipmask = np.logical_or(chipmask, order_stripe)
# for the background we have to invert that mask and (optionally) exclude the top and bottom regions
# which still include fainter orders etc (same as in "extract_background")
if masktype.lower() in ['bg', 'background']:
chipmask = np.invert(chipmask)
if exclude_top_and_bottom:
if debug_level >= 1:
print(
'WARNING: this fix works for the current Veloce CCD layout only!!!'
)
labelled_mask, nobj = label(chipmask)
# WARNING: this fix works for the current Veloce CCD layout only!!!
topleftnumber = labelled_mask[ny - 1, 0]
# toprightnumber = labelled_mask[ny-1,nx-1]
# bottomleftnumber = labelled_mask[0,0]
bottomrightnumber = labelled_mask[0, nx - 1]
chipmask[labelled_mask == topleftnumber] = False
# chipmask[labelled_mask == toprightnumber] = False
chipmask[labelled_mask == bottomrightnumber] = False
return chipmask
def ndimage_alg(self, img, opts):
"""Island detection using scipy.ndimage
Use scipy.ndimage.label to detect islands of emission in the image.
Island is defined as group of tightly connected (8-connectivity
for 2D images) pixels with emission.
The following cuts are applied:
- pixel is considered to have emission if it is 'thresh_isl' times
higher than RMS.
- Island should have at least 'minsize' active pixels
- There should be at lease 1 pixel in the island which is 'thresh_pix'
times higher than noise (peak clip).
Parameters:
image, mask: arrays with image data and mask
mean, rms: arrays with mean & rms maps
thresh_isl: threshold for 'active pixels'
thresh_pix: threshold for peak
minsize: minimal acceptable island size
Function returns a list of Island objects.
"""
### islands detection
mylog = mylogger.logging.getLogger("PyBDSM." + img.log + "Islands")
image = img.ch0_arr
mask = img.mask_arr
rms = img.rms_arr
mean = img.mean_arr
thresh_isl = opts.thresh_isl
thresh_pix = img.thresh_pix
clipped_mean = img.clipped_mean
saverank = opts.savefits_rankim
# act_pixels is true if significant emission
if img.masked:
act_pixels = ~(mask.copy())
act_pixels[~mask] = (image[~mask] -
mean[~mask]) / thresh_isl >= rms[~mask]
else:
act_pixels = (image - mean) / thresh_isl >= rms
# dimension of image
rank = len(image.shape)
# generates matrix for connectivity, in this case, 8-conn
connectivity = nd.generate_binary_structure(rank, rank)
# labels = matrix with value = (initial) island number
labels, count = nd.label(act_pixels, connectivity)
# slices has limits of bounding box of each such island
slices = nd.find_objects(labels)
img.island_labels = labels
### apply cuts on island size and peak value
pyrank = N.zeros(image.shape, dtype=N.int32)
res = []
islid = 0
for idx, s in enumerate(slices):
idx += 1 # nd.labels indices are counted from 1
# number of pixels inside bounding box which are in island
isl_size = (labels[s] == idx).sum()
isl_peak = nd.maximum(image[s], labels[s], idx)
isl_maxposn = tuple(N.array(N.unravel_index(N.nanargmax(image[s]), image[s].shape))+\
N.array((s[0].start, s[1].start)))
if (isl_size >= img.minpix_isl
) and (isl_peak -
mean[isl_maxposn]) / thresh_pix > rms[isl_maxposn]:
isl = Island(image, mask, mean, rms, labels, s, idx,
img.pixel_beamarea())
res.append(isl)
pyrank[tuple(
isl.bbox)] += N.invert(isl.mask_active) * idx // idx
return res
# 4. label the junctions and extract information
#prepares to write in excel
excelpath = parent_dir + "/Outlines.xlsx"
Workbook().save(filename=excelpath)
book = load_workbook(excelpath)
writer = pd.ExcelWriter(excelpath, engine='openpyxl')
writer.book = book
s = [[1, 1, 1], [1, 1, 1], [1, 1, 1]] #structure parameter
print("detecting Outlines...")
for file in sorted(glob.glob(image_j2_dir)): #loops over cut Outlines
read = io.imread(file)
#labels the junctions
mask = read == 1
label_mask, num_labels = ndimage.label(mask, structure=s)
# imwrite(parent_dir+"/Outlines_cut/Outlines_id_"+file[-7:-4]+".tif", np.uint16(label_mask))
# junctionlabels = color.label2rgb(label_mask, bg_label=0)
# plt.imshow(junctionlabels)
#gets info from junctions
props = regionprops_table(
label_mask, properties=['label', 'area', 'coords', "centroid"])
df1 = pd.DataFrame(props)
df1.to_excel(writer, sheet_name="Outline_t" + file[-7:-4])
# centroids2=data2["centroids"].tolist()
print("saved Outlines.xlsx")
writer.save()
writer.close()
#-----------------------------------------------------------------------------
def label(mask):
labeled, nr_true = ndimage.label(mask)
return labeled
def main():
parser = argparse.ArgumentParser()
parser.add_argument('--path_run', help='path to the prediction folder.')
parser.add_argument('--s_min', default=50, type=int, help='min spine size')
parser.add_argument('--s_max',
default=45000,
type=int,
help='max spine size')
parsed_args = parser.parse_args(sys.argv[1:])
path_run = parsed_args.path_run # get prediction folder
s_min = parsed_args.s_min # get min size
s_max = parsed_args.s_max # get max size
path_pred = os.path.join(path_run, "prediction")
# initialization
header = ['FP', 'FN', 'TP', 'Sens.', 'Precision']
FP_global = list()
FN_global = list()
TP_global = list()
SENS_global = list()
PREC_global = list()
FP_d = list()
FN_d = list()
TP_d = list()
SENS_d = list()
PREC_d = list()
threshold_global = list()
validation_cases = list()
for index in range(0, 10, 1): # threshold scan
coinc_thr = index / 10 # from 0 to 1 in 0.1 steps
FP_thr = np.array([])
FN_thr = np.array([])
TP_thr = np.array([])
dir = listdir(path_pred)
for case_folder in dir: # for each case
path, val_case = os.path.split(case_folder)
# load gt and prediction files
truth_file = os.path.join(path_pred, case_folder, "truth.nii.gz")
truth_image = nib.load(truth_file)
truth = truth_image.get_data()
prediction_file = os.path.join(path_pred, case_folder,
"prediction.nii.gz")
prediction_image = nib.load(prediction_file)
prediction = prediction_image.get_data()
''' DENDRITE '''
if index == 0: # run just the first time
print("dendrite case: " + case_folder)
# get dendrite coords fot GT and prediction
den_pred = np.where(prediction == [150])
den_gt = np.where(truth == [150])
# transpose
den_pred_t = np.transpose(np.asarray(den_pred))
den_gt_t = np.transpose(np.asarray(den_gt))
tp_d = 0 # init
# count number of coincident coords
for element in den_pred_t:
find = np.where((den_gt_t == element).all(axis=1))
if find[0].size == 1:
tp_d = tp_d + 1
# calculate evaluation metrics
fp_d = den_pred_t.shape[0] - tp_d
fn_d = den_gt_t.shape[0] - tp_d
# append dendrite metrics
FP_d.append(fp_d)
FN_d.append(fn_d)
TP_d.append(tp_d)
SENS_d.append(tp_d / (tp_d + fn_d))
PREC_d.append(tp_d / (tp_d + fp_d))
validation_cases.append(val_case)
''' SPINE '''
# init
tp_case = 0
fn_case = 0
used_list = list() # already detected spines
# delete dendrite from GT and prediction
prediction[den_pred[0], den_pred[1], den_pred[2]] = 0
truth[den_gt[0], den_gt[1], den_gt[2]] = 0
# get gt and prediction labels
label_prediction, num_labels_prediction = label(prediction)
label_truth, num_labels_truth = label(truth)
# get gt and predictions spines
props_pred = regionprops(label_prediction) # get prediction blobs
props_truth = regionprops(label_truth)
# preprocess prediction spines
for spinePred in range(num_labels_prediction): # for each spine
size = props_pred[spinePred].area # get size
if size <= s_min or size >= s_max: # if not in between thresholds
prediction[
props_pred[spinePred].coords[:, 0],
props_pred[spinePred].coords[:, 1],
props_pred[spinePred].coords[:, 2]] = 0 # delete spine
# get new prediction labels and spines
label_prediction, num_labels_prediction = label(prediction)
props_pred = regionprops(label_prediction)
for spineGT in range(
num_labels_truth): # for each spine in gt (spineGT)
# print progression
prog = (spineGT / num_labels_truth) * 100
print("case: " + case_folder + " - Progreso gt to pred: " +
str(round(prog, 1)) + "%")
# init
coincide_list_GT = list()
coincide_list_Pred = list()
coordsGT = props_truth[spineGT].coords # get spineGT coords
for spinePred in range(
num_labels_prediction
): # for each spine in prediction (spinePred)
# init
counter_sp_coord = 0
coordsPred = props_pred[
spinePred].coords # get spinePred coords
for pos in coordsGT: # for each pixel in SpineGT
find = np.where((coordsPred == pos).all(
axis=1)) # look if it is in spinePred
if find[0].size == 1: # if it is, count 1
counter_sp_coord += 1
# calculate % of pixels found, respect gt and pred size
percentageGT = counter_sp_coord / props_truth[spineGT].area
percentagePred = counter_sp_coord / props_pred[
spinePred].area
# save %
coincide_list_GT.append(percentageGT)
coincide_list_Pred.append(percentagePred)
# delete % from positions of already detected spines
for ind in used_list:
coincide_list_GT[ind] = 0
coincide_list_Pred[ind] = 0
# get maximum mean score
coincide_list_mean = [
(x + y) / 2
for x, y in zip(coincide_list_GT, coincide_list_Pred)
] # scores mean
max_coinc = max(coincide_list_mean) # max mean score
max_index = coincide_list_mean.index(
max_coinc) # max mean score index
# check if spine is detected
if max_coinc > coinc_thr: # if max_coinc is > than coinc_thr
tp_case = tp_case + 1 # tp + 1
used_list.append(max_index) # spine detected
else:
fn_case = fn_case + 1 # if not, fn + 1
fp_case = num_labels_prediction - tp_case # get fp as the difference between detected spines and tp
# save case metrics
FP_thr = np.append(FP_thr, fp_case)
FN_thr = np.append(FN_thr, fn_case)
TP_thr = np.append(TP_thr, tp_case)
# save dendrite results on csv
dendrite_csv = ({
header[0]: FP_d,
header[1]: FN_d,
header[2]: TP_d,
header[3]: SENS_d,
header[4]: PREC_d
})
df = pd.DataFrame.from_records(dendrite_csv, index=validation_cases)
df.to_csv(path_run + "/dendrite_scores.csv")
# calculate and save threshold metrics
FP_sum = np.sum(FP_thr)
FN_sum = np.sum(FN_thr)
TP_sum = np.sum(TP_thr)
SENS_thr = TP_sum / (TP_sum + FN_sum)
PREC_thr = TP_sum / (TP_sum + FP_sum)
FP_global.append(FP_sum)
FN_global.append(FN_sum)
TP_global.append(TP_sum)
SENS_global.append(SENS_thr)
PREC_global.append(PREC_thr)
threshold_global.append(coinc_thr)
# save spine results on csv
spine_csv = ({
header[0]: FP_global,
header[1]: FN_global,
header[2]: TP_global,
header[3]: SENS_global,
header[4]: PREC_global
})
df = pd.DataFrame.from_records(spine_csv, index=threshold_global)
df.to_csv(path_run + "/spine_scores.csv")
def process_signal(audio_plot, audio_signal, parameters, time_stamps):
# partition the audio history into blocks of type:
# 1. noise, where the volume is greater than noise_threshold
# 2. silence, where the volume is less than noise_threshold
noise = audio_signal > parameters['noise_threshold']
silent = audio_signal < parameters['noise_threshold']
# join "noise blocks" that are closer together than min_quiet_time
crying_blocks = []
if np.any(noise):
silent_labels, _ = ndimage.label(silent)
silent_ranges = ndimage.find_objects(silent_labels)
for silent_block in silent_ranges:
start = silent_block[0].start
stop = silent_block[0].stop
# don't join silence blocks at the beginning or end
if start == 0:
continue
interval_length = time_stamps[stop - 1] - time_stamps[start]
if interval_length < parameters['min_quiet_time']:
noise[start:stop] = True
# find noise blocks start times and duration
crying_labels, num_crying_blocks = ndimage.label(noise)
crying_ranges = ndimage.find_objects(crying_labels)
for cry in crying_ranges:
start = time_stamps[cry[0].start]
stop = time_stamps[cry[0].stop - 1]
duration = stop - start
# ignore isolated noises (i.e. with a duration less than min_noise_time)
if duration < parameters['min_noise_time']:
continue
# save some info about the noise block
crying_blocks.append({
'start':
start,
'start_str':
datetime.fromtimestamp(start).strftime("%I:%M:%S %p").lstrip(
'0'),
'stop':
stop,
'duration':
format_time_difference(start, stop)
})
# determine how long have we been in the current state
time_current = time.time()
time_crying = ""
time_quiet = ""
str_crying = "Drone noise for "
str_quiet = "Drone quiet for "
if len(crying_blocks) == 0:
time_quiet = str_quiet + format_time_difference(
time_stamps[0], time_current)
else:
if time_current - crying_blocks[-1]['stop'] < parameters[
'min_quiet_time']:
time_crying = str_crying + format_time_difference(
crying_blocks[-1]['start'], time_current)
else:
time_quiet = str_quiet + format_time_difference(
crying_blocks[-1]['stop'], time_current)
results = {
'audio_plot': audio_plot,
'crying_blocks': crying_blocks,
'time_crying': time_crying,
'time_quiet': time_quiet
}
return results
def fill(self, coord, new_label, refresh=True):
"""Replace an existing label with a new label, either just at the
connected component if the `contiguous` flag is `True` or everywhere
if it is `False`, working either just in the current slice if
the `n_dimensional` flag is `False` or on the entire data if it is
`True`.
Parameters
----------
coord : sequence of float
Position of mouse cursor in image coordinates.
new_label : int
Value of the new label to be filled in.
refresh : bool
Whether to refresh view slice or not. Set to False to batch paint
calls.
"""
int_coord = tuple(np.round(coord).astype(int))
# If requested fill location is outside data shape then return
if np.any(np.less(int_coord, 0)) or np.any(
np.greater_equal(int_coord, self.data.shape)
):
return
# If requested new label doesn't change old label then return
old_label = self.data[int_coord]
if old_label == new_label or (
self.preserve_labels and old_label != self._background_label
):
return
if refresh is True:
self._save_history()
if self.n_dimensional or self.ndim == 2:
# work with entire image
labels = self.data
slice_coord = tuple(int_coord)
else:
# work with just the sliced image
labels = self._data_raw
slice_coord = tuple(int_coord[d] for d in self.dims.displayed)
matches = labels == old_label
if self.contiguous:
# if contiguous replace only selected connected component
labeled_matches, num_features = ndi.label(matches)
if num_features != 1:
match_label = labeled_matches[slice_coord]
matches = np.logical_and(
matches, labeled_matches == match_label
)
# Replace target pixels with new_label
labels[matches] = new_label
if not (self.n_dimensional or self.ndim == 2):
# if working with just the slice, update the rest of the raw data
self.data[tuple(self._slice_indices)] = labels
if refresh is True:
self.refresh()
def DivideInNeighborhoods(data, number_of_spots, scale, sensitivity_limit=100):
"""
Given an image that includes N spots, divides it in N subimages with each of them
to include one spot. Briefly, it filters the image, finds the N “brightest” spots
and crops the region around them generating the subimages. This process is repeated
until image division is feasible.
data (model.DataArray): 2D array containing the intensity of each pixel
number_of_spots (int,int): The number of CL spots
scale (float): Distance between spots in optical grid (in pixels)
sensitivity_limit (int): Limit of sensitivity
returns subimages (List of DataArrays): One subimage per spot
subimage_coordinates (List of tuples): The coordinates of the center of each
subimage with respect to the overall image
"""
# Denoise
filtered_image = ndimage.median_filter(data, 3)
# Bold spots
# Third parameter must be a length in pixels somewhat larger than a typical
# spot
filtered_image = _BandPassFilter(filtered_image, 1, 20)
image = model.DataArray(filtered_image, data.metadata)
avg_intensity = numpy.average(image)
spot_factor = 10
step = 1
sensitivity = 4
# After filtering based on optical scale there is no need to adjust
# filter window size
filter_window_size = 8
# Increase sensitivity until expected number of spots is detected
while sensitivity <= sensitivity_limit:
subimage_coordinates = []
subimages = []
i_max, j_max = unravel_index(image.argmax(), image.shape)
i_min, j_min = unravel_index(image.argmin(), image.shape)
max_diff = image[i_max, j_max] - image[i_min, j_min]
data_max = filters.maximum_filter(image, filter_window_size)
data_min = filters.minimum_filter(image, filter_window_size)
# Determine threshold
i = sensitivity
threshold = max_diff / i
# Filter the parts of the image with variance in intensity greater
# than the threshold
maxima = (image == data_max)
diff = ((data_max - data_min) > threshold)
maxima[diff == 0] = 0
labeled, num_objects = ndimage.label(maxima)
slices = ndimage.find_objects(labeled)
(x_center_last, y_center_last) = (-10, -10)
# Go through these parts and crop the subimages based on the neighborhood_size
# value
for dy, dx in slices:
x_center = (dx.start + dx.stop - 1) / 2
y_center = (dy.start + dy.stop - 1) / 2
# Make sure we don't detect spots on the top of each other
tab = tuple(
map(operator.sub, (x_center_last, y_center_last),
(x_center, y_center)))
subimage = image[int(dy.start - 2.5):int(dy.stop + 2.5),
int(dx.start - 2.5):int(dx.stop + 2.5)]
if subimage.shape[0] == 0 or subimage.shape[1] == 0:
continue
if (subimage > spot_factor * avg_intensity).sum() < 6:
continue
# if spots detected too close keep the brightest one
if (len(subimages) > 0) and (math.hypot(tab[0], tab[1]) <
(scale / 2)):
if numpy.sum(subimage) > numpy.sum(
subimages[len(subimages) - 1]):
subimages.pop()
subimage_coordinates.pop()
subimage_coordinates.append((x_center, y_center))
subimages.append(subimage)
else:
subimage_coordinates.append((x_center, y_center))
subimages.append(subimage)
(x_center_last, y_center_last) = (x_center, y_center)
# Take care of outliers
expected_spots = numpy.prod(number_of_spots)
clean_subimages, clean_subimage_coordinates = FilterOutliers(
image, subimages, subimage_coordinates, expected_spots)
if len(clean_subimages) >= numpy.prod(number_of_spots):
break
if sensitivity > 4:
step = 4
sensitivity += step
return clean_subimages, clean_subimage_coordinates
def join_bins(bin_edges, probabilities, min_prob=0.05):
"""Join bins until at least the minimum probability is contained.
By joining adjacent bins, find the configuration with the maximum
number of bins that each contain at least a certain probability.
Parameters
----------
bin_edges : iterable of 2-tuple
Upper and lower bounds associated with each bin.
probabilities : array-like
Probabilities in each bin. The contiguous ranges where bin joining
must be attempted will automatically be determined.
min_prob : float
The minimum probability (inclusive) per (final) bin.
Returns
-------
bin_edges : list of 2-tuple
List containing the new bin edges.
probabilities : array
Probabilities corresponding to the above bins.
Raises
------
ValueError : If the number of bin edges does not match the number
of probabilities.
ValueError : If the sum of all `probabilities` does not exceed `min_prob`.
"""
if len(bin_edges) != len(probabilities):
raise ValueError("Length of bin_edges and probabilities must match.")
probabilities = np.asarray(probabilities)
if np.sum(probabilities) <= min_prob:
raise ValueError("Sum of probabilities must exceed min_prob.")
max_i = probabilities.shape[0] - 1
def _join(start_i, end_i):
"""Return new bin edges and probabilities after a join.
Parameters
----------
start_i : int
Beginning of the join.
end_i : int
End of the join.
Returns
-------
bin_edges : list of 2-tuple
List containing the new bin edges.
probabilities : array
Probabilities corresponding to the above bins.
"""
new_bin_edges = []
new_probabilities = []
# Remove all but the first index within the join window.
for i in filterfalse(
lambda x: x in range(start_i + 1, end_i + 1), range(max_i + 1),
):
if i == start_i:
new_bin_edges.append((bin_edges[start_i][0], bin_edges[end_i][1],))
new_probabilities.append(np.sum(probabilities[start_i : end_i + 1]))
else:
new_bin_edges.append(bin_edges[i])
new_probabilities.append(probabilities[i])
return join_bins(
bin_edges=new_bin_edges, probabilities=new_probabilities, min_prob=min_prob,
)
# Identify regions with low probabilities.
join_mask = probabilities < min_prob
if not np.any(join_mask):
# Joining is complete.
return (bin_edges, probabilities)
# Find the contiguous clusters.
labelled, n_clusters = label(join_mask)
variations = []
# Carry out contiguous bin joining around all clusters.
for cluster_i in range(1, n_clusters + 1):
cluster_indices = np.where(labelled == cluster_i)[0]
cluster_bounds = (cluster_indices[0], cluster_indices[-1])
# Also consider the adjacent bins, since this may be needed
# in some cases.
join_bounds = tuple(
np.clip(np.array([cluster_bounds[0] - 1, cluster_bounds[1] + 1]), 0, max_i)
)
for start_i in range(*join_bounds):
# Optimisation: prevent 'orphan' bins on the left.
if join_bounds[0] == 0 and start_i != 0:
continue
for end_i in range(start_i + 1, join_bounds[1] + 1):
# Optimisation: prevent 'orphan' bins on the right.
if join_bounds[1] == max_i and end_i != max_i:
continue
# If the sum of probabilities between `start_i` and `end_i`
# exceeds the minimum threshold, join the bins.
if np.sum(probabilities[start_i : end_i + 1]) >= min_prob:
variations.append(_join(start_i, end_i))
# Return the 'best' variation - the set of bins with the lowest variability,
# measured using the standard deviation of the probabilities. Only sets with
# the largest number of bins will be considered here.
lengths = [len(variation[0]) for variation in variations]
max_length = max(lengths)
long_variations = [
variation for variation in variations if len(variation[0]) == max_length
]
variation_stds = [np.std(variation[1]) for variation in long_variations]
min_index = np.argsort(variation_stds)[0]
return long_variations[min_index]
def _detect_sources(data, thresholds, npixels, filter_kernel=None,
connectivity=8, mask=None, deblend_skip=False):
"""
Detect sources above a specified threshold value in an image and
return a `~photutils.segmentation.SegmentationImage` object.
Detected sources must have ``npixels`` connected pixels that are
each greater than the ``threshold`` value. If the filtering option
is used, then the ``threshold`` is applied to the filtered image.
The input ``mask`` can be used to mask pixels in the input data.
Masked pixels will not be included in any source.
This function does not deblend overlapping sources. First use this
function to detect sources followed by
:func:`~photutils.segmentation.deblend_sources` to deblend sources.
Parameters
----------
data : array_like
The 2D array of the image.
thresholds : array-like of floats or arrays
The data value or pixel-wise data values to
be used for the detection thresholds. A 2D
``threshold`` must have the same shape as ``data``. See
`~photutils.segmentation.detect_threshold` for one way to create
a ``threshold`` image.
npixels : int
The number of connected pixels, each greater than ``threshold``,
that an object must have to be detected. ``npixels`` must be a
positive integer.
filter_kernel : array-like (2D) or `~astropy.convolution.Kernel2D`, optional
The 2D array of the kernel used to filter the image before
thresholding. Filtering the image will smooth the noise and
maximize detectability of objects with a shape similar to the
kernel.
connectivity : {4, 8}, optional
The type of pixel connectivity used in determining how pixels
are grouped into a detected source. The options are 4 or
8 (default). 4-connected pixels touch along their edges.
8-connected pixels touch along their edges or corners. For
reference, SourceExtractor uses 8-connected pixels.
mask : array_like of bool, optional
A boolean mask, with the same shape as the input ``data``, where
`True` values indicate masked pixels. Masked pixels will not be
included in any source.
deblend_skip : bool, optional
If `True` do not include the segmentation image in the output
list for any threshold level where the number of detected
sources is less than 2. This is useful for source deblending
and improves its performance.
Returns
-------
segment_image : list of `~photutils.segmentation.SegmentationImage`
A list of 2D segmentation images, with the same shape as
``data``, where sources are marked by different positive integer
values. A value of zero is reserved for the background. If no
sources are found for a given threshold, then the output list
will contain `None` for that threshold. Also see the
``deblend_skip`` keyword.
"""
from scipy import ndimage
if (npixels <= 0) or (int(npixels) != npixels):
raise ValueError('npixels must be a positive integer, got '
f'"{npixels}"')
if mask is not None:
if mask.shape != data.shape:
raise ValueError('mask must have the same shape as the input '
'image.')
if filter_kernel is not None:
data = _filter_data(data, filter_kernel, mode='constant',
fill_value=0.0, check_normalization=True)
selem = _make_binary_structure(data.ndim, connectivity)
segms = []
for threshold in thresholds:
# ignore RuntimeWarning caused by > comparison when data contains NaNs
with warnings.catch_warnings():
warnings.simplefilter('ignore', category=RuntimeWarning)
data2 = data > threshold
if mask is not None:
data2 &= ~mask
# return if threshold was too high to detect any sources
if np.count_nonzero(data2) == 0:
warnings.warn('No sources were found.', NoDetectionsWarning)
if deblend_skip:
continue
else:
segms.append(None)
continue
segm_img, _ = ndimage.label(data2, structure=selem)
# remove objects with less than npixels
# NOTE: for typical data, making the cutout images is ~10x faster
# than using segm_img directly
segm_slices = ndimage.find_objects(segm_img)
for i, slices in enumerate(segm_slices):
cutout = segm_img[slices]
segment_mask = (cutout == (i + 1))
if np.count_nonzero(segment_mask) < npixels:
cutout[segment_mask] = 0
if np.count_nonzero(segm_img) == 0:
warnings.warn('No sources were found.', NoDetectionsWarning)
if deblend_skip:
continue
else:
segms.append(None)
continue
segm = object.__new__(SegmentationImage)
segm._data = segm_img
if deblend_skip and segm.nlabels == 1:
continue
else:
segm.relabel_consecutive()
segms.append(segm)
return segms
def segment_glomeruli3d(input_dir, output_dir, slice_names):
"""
Original implementation from Anna's script
:param input_dir:
:param output_dir:
:param slice_names:
:param voxel_xy:
:param voxel_z:
:return:
"""
if not os.path.exists(output_dir):
os.makedirs(output_dir)
num = 0
mask = io.imread(input_dir + "/" + slice_names[0])
l0 = np.zeros_like(mask)
labels = []
limsize = int(float(glomeruli_maxrad))
nfiles = [output_dir + "/" + x for x in slice_names]
# label
for i in range(len(slice_names)):
mask = io.imread(input_dir + "/" + slice_names[i])
l, n = ndimage.label(mask)
labels.append(np.zeros_like(l))
for j in range(n): # FOR EACH label
ind = np.where(l == j + 1) # Indices Of the current label
ls = np.unique(l0[ind])
ls = ls[np.where(
ls > 0
)] # Get the connections current_label -> last_layer_label
if len(ls) == 0:
num += 1
labels[-1][ind] = num
if len(ls) == 1:
labels[-1][ind] = ls[0]
if len(ls) > 1:
labels[-1][ind] = ls[0] # Assign to target (first encountered)
for k in range(1, len(ls)): # FOREACH all other connections
for s in range(len(
labels)): # Go through all layers and remove them
labels[s] = np.where(labels[s] == ls[k], ls[0],
labels[s])
l0 = labels[-1]
# if number of layers exceeds limit, save the first layer
if len(labels) > limsize:
mask = labels.pop(0)
fname = nfiles.pop(0)
tifffile.imsave(fname, img_as_int(mask), compress=5)
# save remaining layers
for i in range(len(labels)):
mask = labels.pop(0)
fname = nfiles.pop(0)
tifffile.imsave(fname, img_as_int(mask), compress=5)
async def setup_learner():
await download_file(export_file_url, path / export_file_name)
try:
img_new = cv2.imread(str(path), cv2.IMREAD_COLOR)
img_new = cv2.fastNlMeansDenoisingColored(img_new, None, 10, 10, 7, 21)
img_sv = img_new
img_test = img_sv
img = img_new
r = img.copy()
r[:, :, 0] = 0
r[:, :, 1] = 0
img = r
img = cv2.cvtColor(img, cv2.COLOR_BGR2RGB)
test_r = img
im_gray = cv2.cvtColor(r, cv2.COLOR_BGR2GRAY)
im_blur = cv2.GaussianBlur(im_gray, (5, 5), 0)
kernel_sharpening = np.array([[-1, -1, -1], [-1, 9, -1], [-1, -1, -1]])
im_sharp = cv2.filter2D(im_blur, -1, kernel_sharpening)
img = im_sharp
th3 = cv2.adaptiveThreshold(img, 255, cv2.ADAPTIVE_THRESH_GAUSSIAN_C,
cv2.THRESH_BINARY, 11, 2)
img = im_sharp
ret, thresh3 = cv2.threshold(img, 127, 255, cv2.THRESH_TRUNC)
kernel = np.ones((3, 3), np.uint8)
opening = cv2.morphologyEx(thresh3,
cv2.MORPH_OPEN,
kernel,
iterations=2)
ret, th = cv2.threshold(thresh3, 0, 255,
cv2.THRESH_BINARY + cv2.THRESH_OTSU)
kernel = np.ones((3, 3), np.uint8)
opening = cv2.morphologyEx(th, cv2.MORPH_OPEN, kernel, iterations=2)
sure_bg = cv2.dilate(th, kernel, iterations=3)
dist_transform = cv2.distanceTransform(opening, cv2.DIST_L2, 5)
ret, sure_fg = cv2.threshold(dist_transform,
0.005 * dist_transform.max(), 255, 0)
sure_fg = np.uint8(sure_fg)
unknown = cv2.subtract(sure_bg, sure_fg)
ret, markers = cv2.connectedComponents(sure_fg)
markers = markers + 1
markers[unknown == 255] = 0
markers = cv2.watershed(img_sv, markers)
img_sv[markers == -1] = [0, 255, 0]
img_sv[markers == 1] = [0, 0, 0]
from skimage.filters import threshold_otsu
thresh_val = threshold_otsu(opening)
mask = np.where(opening > thresh_val, 1, 0)
if np.sum(mask == 0) < np.sum(mask == 1):
mask = np.where(mask, 0, 1)
from scipy import ndimage
labels, nlabels = ndimage.label(mask)
all_cords = {}
for label_ind, label_coords in enumerate(ndimage.find_objects(labels)):
cell = im_gray[label_coords]
all_cords[label_ind] = label_coords
if np.product(cell.shape) < 10:
mask = np.where(labels == label_ind + 1, 0, mask)
labels, nlabels = ndimage.label(mask)
i = 0
res = {}
for ii, obj_indices in enumerate(
ndimage.find_objects(labels)[0:nlabels]):
cell = img_sv[obj_indices]
res[i] = cell
i = i + 1
i = 0
features = {}
for i in res:
gcolor = res[i]
h, w, _ = gcolor.shape
ggray = np.array([[gcolor[i, j, 2] for j in range(w)]
for i in range(h)],
dtype=np.uint8)
area = np.sum(
np.sum([[1.0 for j in range(w) if ggray[i, j]]
for i in range(h)]))
mean_area = area / (h * w)
r, b, g = np.sum(
[gcolor[i, j] for j in range(w)
for i in range(h)], axis=0) / (area * 256)
_, _, eigen_value = pca(ggray)
eccentricity = eigen_value[0] / eigen_value[1]
l = [
mean_area, r, b, g, eigen_value[0], eigen_value[1],
eccentricity
]
features[i] = np.array(l)
i = i + 1
out = {}
#learn = load_learner(path, export_file_name)
#Change Working directory of pkl file
model = keras.models.load_model('../input/weight/weights.pkl')
out = {}
for i in features:
out[i] = model.predict(np.array([features[i]]))
good = not_good = 0
for i in out:
s = res[i]
if np.argmax(out[i][0]) == 0:
good += 1
x1 = all_cords[i][0].start
y1 = all_cords[i][1].start
x2 = all_cords[i][1].stop
y2 = all_cords[i][0].stop
cv2.rectangle(img_test, (x2, x1), (y1, y2), (255, 0, 0), 8)
else:
x1 = all_cords[i][0].start
y1 = all_cords[i][1].start
x2 = all_cords[i][1].stop
y2 = all_cords[i][0].stop
not_good += 1
cv2.rectangle(img_test, (x2, x1), (y1, y2), (0, 0, 255), 3)
p = (good / (good + not_good) * 100)
print("Number of good grain :", good)
print("Number of impure grains or impurity:", not_good)
print("Percentage Purity is:", p)
return p
except RuntimeError as e:
if len(e.args) > 0 and 'CPU-only machine' in e.args[0]:
print(e)
message = "\n\nThis model was trained with an old version of fastai and will not work in a CPU environment.\n\nPlease update the fastai library in your training environment and export your model again.\n\nSee instructions for 'Returning to work' at https://course.fast.ai."
raise RuntimeError(message)
else:
raise
def test_bundle_of_tubes(self):
im = ps.generators.bundle_of_tubes(shape=[101, 101, 1], spacing=10)
labels, N = spim.label(input=im)
assert N == 100
def test_lattice_spheres_triangular(self):
im = ps.generators.lattice_spheres(shape=[101, 101],
radius=5,
lattice='triangular')
labels, N = spim.label(input=~im)
assert N == 85
def regionprops(label_image,
properties=['Area', 'Centroid'],
intensity_image=None):
"""Measure properties of labelled image regions.
Parameters
----------
label_image : (N, M) ndarray
Labelled input image.
properties : {'all', list}
Shape measurements to be determined for each labelled image region.
Default is `['Area', 'Centroid']`. The following properties can be
determined:
* Area : int
Number of pixels of region.
* BoundingBox : tuple
Bounding box `(min_row, min_col, max_row, max_col)`
* CentralMoments : (3, 3) ndarray
Central moments (translation invariant) up to 3rd order.
mu_ji = sum{ array(x, y) * (x - x_c)^j * (y - y_c)^i }
where the sum is over the `x`, `y` coordinates of the region,
and `x_c` and `y_c` are the coordinates of the region's centroid.
* Centroid : array
Centroid coordinate tuple `(row, col)`.
* ConvexArea : int
Number of pixels of convex hull image.
* ConvexImage : (H, J) ndarray
Binary convex hull image which has the same size as bounding box.
* Coordinates : (N, 2) ndarray
Coordinate list `(row, col)` of the region.
* Eccentricity : float
Eccentricity of the ellipse that has the same second-moments as the
region. The eccentricity is the ratio of the distance between its
minor and major axis length. The value is between 0 and 1.
* EquivDiameter : float
The diameter of a circle with the same area as the region.
* EulerNumber : int
Euler number of region. Computed as number of objects (= 1)
subtracted by number of holes (8-connectivity).
* Extent : float
Ratio of pixels in the region to pixels in the total bounding box.
Computed as `Area / (rows*cols)`
* FilledArea : int
Number of pixels of filled region.
* FilledImage : (H, J) ndarray
Binary region image with filled holes which has the same size as
bounding box.
* HuMoments : tuple
Hu moments (translation, scale and rotation invariant).
* Image : (H, J) ndarray
Sliced binary region image which has the same size as bounding box.
* MajorAxisLength : float
The length of the major axis of the ellipse that has the same
normalized second central moments as the region.
* MaxIntensity: float
Value with the greatest intensity in the region.
* MeanIntensity: float
Value with the mean intensity in the region.
* MinIntensity: float
Value with the least intensity in the region.
* MinorAxisLength : float
The length of the minor axis of the ellipse that has the same
normalized second central moments as the region.
* Moments : (3, 3) ndarray
Spatial moments up to 3rd order.
m_ji = sum{ array(x, y) * x^j * y^i }
where the sum is over the `x`, `y` coordinates of the region.
* NormalizedMoments : (3, 3) ndarray
Normalized moments (translation and scale invariant) up to 3rd
order.
nu_ji = mu_ji / m_00^[(i+j)/2 + 1]
where `m_00` is the zeroth spatial moment.
* Orientation : float
Angle between the X-axis and the major axis of the ellipse that has
the same second-moments as the region. Ranging from `-pi/2` to
`pi/2` in counter-clockwise direction.
* Perimeter : float
Perimeter of object which approximates the contour as a line
through the centers of border pixels using a 4-connectivity.
* Solidity : float
Ratio of pixels in the region to pixels of the convex hull image.
* WeightedCentralMoments : (3, 3) ndarray
Central moments (translation invariant) of intensity image up to
3rd order.
wmu_ji = sum{ array(x, y) * (x - x_c)^j * (y - y_c)^i }
where the sum is over the `x`, `y` coordinates of the region,
and `x_c` and `y_c` are the coordinates of the region's centroid.
* WeightedCentroid : array
Centroid coordinate tuple `(row, col)` weighted with intensity
image.
* WeightedHuMoments : tuple
Hu moments (translation, scale and rotation invariant) of intensity
image.
* WeightedMoments : (3, 3) ndarray
Spatial moments of intensity image up to 3rd order.
wm_ji = sum{ array(x, y) * x^j * y^i }
where the sum is over the `x`, `y` coordinates of the region.
* WeightedNormalizedMoments : (3, 3) ndarray
Normalized moments (translation and scale invariant) of intensity
image up to 3rd order.
wnu_ji = wmu_ji / wm_00^[(i+j)/2 + 1]
where `wm_00` is the zeroth spatial moment (intensity-weighted
area).
intensity_image : (N, M) ndarray, optional
Intensity image with same size as labelled image. Default is None.
Returns
-------
properties : list of dicts
List containing a property dict for each region. The property dicts
contain all the specified properties plus a 'Label' field.
References
----------
.. [1] Wilhelm Burger, Mark Burge. Principles of Digital Image Processing:
Core Algorithms. Springer-Verlag, London, 2009.
.. [2] B. Jähne. Digital Image Processing. Springer-Verlag,
Berlin-Heidelberg, 6. edition, 2005.
.. [3] T. H. Reiss. Recognizing Planar Objects Using Invariant Image
Features, from Lecture notes in computer science, p. 676. Springer,
Berlin, 1993.
.. [4] http://en.wikipedia.org/wiki/Image_moment
Examples
--------
>>> from skimage.data import coins
>>> from skimage.morphology import label
>>> img = coins() > 110
>>> label_img = label(img)
>>> props = regionprops(label_img)
>>> props[0]['Centroid'] # centroid of first labelled object
"""
if not np.issubdtype(label_image.dtype, 'int'):
raise TypeError('labelled image must be of integer dtype')
# determine all properties if nothing specified
if properties == 'all':
properties = PROPS
props = []
objects = ndimage.find_objects(label_image)
for i, sl in enumerate(objects):
label = i + 1
# create property dict for current label
obj_props = {}
props.append(obj_props)
obj_props['Label'] = label
array = (label_image[sl] == label).astype('double')
# upper left corner of object bbox
r0 = sl[0].start
c0 = sl[1].start
m = _moments.central_moments(array, 0, 0, 3)
# centroid
cr = m[0, 1] / m[0, 0]
cc = m[1, 0] / m[0, 0]
mu = _moments.central_moments(array, cr, cc, 3)
# elements of the inertia tensor [a b; b c]
a = mu[2, 0] / mu[0, 0]
b = mu[1, 1] / mu[0, 0]
c = mu[0, 2] / mu[0, 0]
# eigen values of inertia tensor
l1 = (a + c) / 2 + sqrt(4 * b**2 + (a - c)**2) / 2
l2 = (a + c) / 2 - sqrt(4 * b**2 + (a - c)**2) / 2
# cached results which are used by several properties
_filled_image = None
_convex_image = None
_nu = None
if 'Area' in properties:
obj_props['Area'] = m[0, 0]
if 'BoundingBox' in properties:
obj_props['BoundingBox'] = (r0, c0, sl[0].stop, sl[1].stop)
if 'Centroid' in properties:
obj_props['Centroid'] = cr + r0, cc + c0
if 'CentralMoments' in properties:
obj_props['CentralMoments'] = mu
if 'ConvexArea' in properties:
if _convex_image is None:
_convex_image = convex_hull_image(array)
obj_props['ConvexArea'] = np.sum(_convex_image)
if 'ConvexImage' in properties:
if _convex_image is None:
_convex_image = convex_hull_image(array)
obj_props['ConvexImage'] = _convex_image
if 'Coordinates' in properties:
rr, cc = np.nonzero(array)
obj_props['Coordinates'] = np.vstack((rr + r0, cc + c0)).T
if 'Eccentricity' in properties:
if l1 == 0:
obj_props['Eccentricity'] = 0
else:
obj_props['Eccentricity'] = sqrt(1 - l2 / l1)
if 'EquivDiameter' in properties:
obj_props['EquivDiameter'] = sqrt(4 * m[0, 0] / PI)
if 'EulerNumber' in properties:
if _filled_image is None:
_filled_image = ndimage.binary_fill_holes(array, STREL_8)
euler_array = _filled_image != array
_, num = ndimage.label(euler_array, STREL_8)
obj_props['EulerNumber'] = -num
if 'Extent' in properties:
obj_props['Extent'] = m[0, 0] / (array.shape[0] * array.shape[1])
if 'HuMoments' in properties:
if _nu is None:
_nu = _moments.normalized_moments(mu, 3)
obj_props['HuMoments'] = _moments.hu_moments(_nu)
if 'Image' in properties:
obj_props['Image'] = array
if 'FilledArea' in properties:
if _filled_image is None:
_filled_image = ndimage.binary_fill_holes(array, STREL_8)
obj_props['FilledArea'] = np.sum(_filled_image)
if 'FilledImage' in properties:
if _filled_image is None:
_filled_image = ndimage.binary_fill_holes(array, STREL_8)
obj_props['FilledImage'] = _filled_image
if 'MajorAxisLength' in properties:
obj_props['MajorAxisLength'] = 4 * sqrt(l1)
if 'MinorAxisLength' in properties:
obj_props['MinorAxisLength'] = 4 * sqrt(l2)
if 'Moments' in properties:
obj_props['Moments'] = m
if 'NormalizedMoments' in properties:
if _nu is None:
_nu = _moments.normalized_moments(mu, 3)
obj_props['NormalizedMoments'] = _nu
if 'Orientation' in properties:
if a - c == 0:
if b > 0:
obj_props['Orientation'] = -PI / 4.
else:
obj_props['Orientation'] = PI / 4.
else:
obj_props['Orientation'] = -0.5 * atan2(2 * b, (a - c))
if 'Perimeter' in properties:
obj_props['Perimeter'] = perimeter(array, 4)
if 'Solidity' in properties:
if _convex_image is None:
_convex_image = convex_hull_image(array)
obj_props['Solidity'] = m[0, 0] / np.sum(_convex_image)
if intensity_image is not None:
weighted_array = array * intensity_image[sl]
wm = _moments.central_moments(weighted_array, 0, 0, 3)
# weighted centroid
wcr = wm[0, 1] / wm[0, 0]
wcc = wm[1, 0] / wm[0, 0]
wmu = _moments.central_moments(weighted_array, wcr, wcc, 3)
# cached results which are used by several properties
_wnu = None
_vals = None
if 'MaxIntensity' in properties:
if _vals is None:
_vals = weighted_array[array.astype('bool')]
obj_props['MaxIntensity'] = np.max(_vals)
if 'MeanIntensity' in properties:
if _vals is None:
_vals = weighted_array[array.astype('bool')]
obj_props['MeanIntensity'] = np.mean(_vals)
if 'MinIntensity' in properties:
if _vals is None:
_vals = weighted_array[array.astype('bool')]
obj_props['MinIntensity'] = np.min(_vals)
if 'WeightedCentralMoments' in properties:
obj_props['WeightedCentralMoments'] = wmu
if 'WeightedCentroid' in properties:
obj_props['WeightedCentroid'] = wcr + r0, wcc + c0
if 'WeightedHuMoments' in properties:
if _wnu is None:
_wnu = _moments.normalized_moments(wmu, 3)
obj_props['WeightedHuMoments'] = _moments.hu_moments(_wnu)
if 'WeightedMoments' in properties:
obj_props['WeightedMoments'] = wm
if 'WeightedNormalizedMoments' in properties:
if _wnu is None:
_wnu = _moments.normalized_moments(wmu, 3)
obj_props['WeightedNormalizedMoments'] = _wnu
return props
# convert the mean shift image to grayscale, then apply
# Otsu's thresholding
gray = cv2.cvtColor(shifted, cv2.COLOR_BGR2GRAY)
thresh = cv2.threshold(gray, 0, 255, cv2.THRESH_BINARY | cv2.THRESH_OTSU)[1]
thresh = cv2.copyMakeBorder(thresh, 1, 1, 1, 1, cv2.BORDER_CONSTANT, 0)
#cv2.imshow("Thresh", thresh)
# compute the exact Euclidean distance from every binary
# pixel to the nearest zero pixel, then find peaks in this
# distance map
D = ndimage.distance_transform_edt(thresh)
localMax = peak_local_max(D, indices=False, min_distance=20, labels=thresh)
# perform a connected component analysis on the local peaks,
# using 8-connectivity, then appy the Watershed algorithm
markers = ndimage.label(localMax, structure=np.ones((3, 3)))[0]
labels = watershed(-D, markers, mask=thresh)
print("[INFO] {} unique segments found".format(len(np.unique(labels)) - 1))
circles = np.empty((0, 3), int)
# loop over the unique labels returned by the Watershed
# algorithm
for label in np.unique(labels):
# if the label is zero, we are examining the 'background'
# so simply ignore it
if label == 0:
continue
# otherwise, allocate memory for the label region and draw
# it on the mask
def extract_particles(self, segmentation):
"""
Saves particle centers into output .star file, after dismissing regions
that are too big to contain a particle.
Args:
segmentation: Segmentation of the micrograph into noise and particle projections.
"""
segmentation = segmentation[self.query_size // 2 - 1:-self.query_size // 2,
self.query_size // 2 - 1:-self.query_size // 2]
labeled_segments, _ = ndimage.label(segmentation, np.ones((3, 3)))
values, repeats = np.unique(labeled_segments, return_counts=True)
values_to_remove = np.where(repeats > self.max_size ** 2)
values = np.take(values, values_to_remove)
values = np.reshape(values, (1, 1, np.prod(values.shape)), 'F')
labeled_segments = np.reshape(labeled_segments, (labeled_segments.shape[0],
labeled_segments.shape[1], 1), 'F')
matrix1 = np.repeat(labeled_segments, values.shape[2], 2)
matrix2 = np.repeat(values, matrix1.shape[0], 0)
matrix2 = np.repeat(matrix2, matrix1.shape[1], 1)
matrix3 = np.equal(matrix1, matrix2)
matrix4 = np.sum(matrix3, 2)
segmentation[np.where(matrix4 == 1)] = 0
labeled_segments, _ = ndimage.label(segmentation, np.ones((3, 3)))
max_val = np.amax(np.reshape(labeled_segments, (np.prod(labeled_segments.shape))))
center = center_of_mass(segmentation, labeled_segments, np.arange(1, max_val))
center = np.rint(center)
img = np.zeros((segmentation.shape[0], segmentation.shape[1]))
img[center[:, 0].astype(int), center[:, 1].astype(int)] = 1
y, x = np.ogrid[-self.moa:self.moa+1, -self.moa:self.moa+1]
element = x*x+y*y <= self.moa * self.moa
img = binary_dilation(img, structure=element)
labeled_img, _ = ndimage.label(img, np.ones((3, 3)))
values, repeats = np.unique(labeled_img, return_counts=True)
y = np.where(repeats == np.count_nonzero(element))
y = np.array(y)
y = y.astype(int)
y = np.reshape(y, (np.prod(y.shape)), 'F')
y -= 1
center = center[y, :]
center = center + (self.query_size // 2 - 1) * np.ones(center.shape)
center = center + (self.query_size // 2 - 1) * np.ones(center.shape)
center = center + np.ones(center.shape)
center = config.apple.mrc_shrink_factor * center
# swap columns to align with Relion
center = center[:, [1, 0]]
# first column is x; second column is y - offset by margins that were discarded from the image
center[:, 0] += config.apple.mrc_margin_left
center[:, 1] += config.apple.mrc_margin_top
if self.output_directory is not None:
basename = os.path.basename(self.filename)
name_str, ext = os.path.splitext(basename)
applepick_path = os.path.join(self.output_directory, "{}_applepick.star".format(name_str))
with open(applepick_path, "w") as f:
np.savetxt(f, ["data_root\n\nloop_\n_rlnCoordinateX #1\n_rlnCoordinateY #2"], fmt='%s')
np.savetxt(f, center, fmt='%d %d')
return center
def pull_spots(self,
plane_data,
grain_params,
imgser_dict,
tth_tol=0.25,
eta_tol=1.,
ome_tol=1.,
npdiv=2,
threshold=10,
eta_ranges=[
(-np.pi, np.pi),
],
ome_period=(-np.pi, np.pi),
dirname='results',
filename=None,
output_format='text',
save_spot_list=False,
quiet=True,
check_only=False,
interp='nearest'):
"""
Exctract reflection info from a rotation series encoded as an
OmegaImageseries object
"""
# grain parameters
rMat_c = makeRotMatOfExpMap(grain_params[:3])
tVec_c = grain_params[3:6]
# grab omega ranges from first imageseries
#
# WARNING: all imageseries AND all wedges within are assumed to have
# the same omega values; put in a check that they are all the same???
oims0 = imgser_dict[imgser_dict.keys()[0]]
ome_ranges = [
np.radians([i['ostart'], i['ostop']])
for i in oims0.omegawedges.wedges
]
# delta omega in DEGREES grabbed from first imageseries in the dict
delta_ome = oims0.omega[0, 1] - oims0.omega[0, 0]
# make omega grid for frame expansion around reference frame
# in DEGREES
ndiv_ome, ome_del = make_tolerance_grid(
delta_ome,
ome_tol,
1,
adjust_window=True,
)
# generate structuring element for connected component labeling
if ndiv_ome == 1:
label_struct = ndimage.generate_binary_structure(2, 2)
else:
label_struct = ndimage.generate_binary_structure(3, 3)
# simulate rotation series
sim_results = self.simulate_rotation_series(plane_data, [
grain_params,
],
eta_ranges=eta_ranges,
ome_ranges=ome_ranges,
ome_period=ome_period)
# patch vertex generator (global for instrument)
tol_vec = 0.5 * np.radians([
-tth_tol, -eta_tol, -tth_tol, eta_tol, tth_tol, eta_tol, tth_tol,
-eta_tol
])
# prepare output if requested
if filename is not None and output_format.lower() == 'hdf5':
this_filename = os.path.join(dirname, filename)
writer = io.GrainDataWriter_h5(os.path.join(dirname, filename),
self.write_config(), grain_params)
# =====================================================================
# LOOP OVER PANELS
# =====================================================================
iRefl = 0
compl = []
output = dict.fromkeys(self.detectors)
for detector_id in self.detectors:
# initialize text-based output writer
if filename is not None and output_format.lower() == 'text':
output_dir = os.path.join(dirname, detector_id)
if not os.path.exists(output_dir):
os.makedirs(output_dir)
this_filename = os.path.join(output_dir, filename)
writer = io.PatchDataWriter(this_filename)
# grab panel
panel = self.detectors[detector_id]
instr_cfg = panel.config_dict(self.chi, self.tvec)
native_area = panel.pixel_area # pixel ref area
# pull out the OmegaImageSeries for this panel from input dict
ome_imgser = imgser_dict[detector_id]
# extract simulation results
sim_results_p = sim_results[detector_id]
hkl_ids = sim_results_p[0][0]
hkls_p = sim_results_p[1][0]
ang_centers = sim_results_p[2][0]
xy_centers = sim_results_p[3][0]
ang_pixel_size = sim_results_p[4][0]
# now verify that full patch falls on detector...
# ???: strictly necessary?
#
# patch vertex array from sim
nangs = len(ang_centers)
patch_vertices = (np.tile(ang_centers[:, :2],
(1, 4)) + np.tile(tol_vec,
(nangs, 1))).reshape(
4 * nangs, 2)
ome_dupl = np.tile(ang_centers[:, 2],
(4, 1)).T.reshape(len(patch_vertices), 1)
# find vertices that all fall on the panel
det_xy, _ = xrdutil._project_on_detector_plane(
np.hstack([patch_vertices, ome_dupl]), panel.rmat, rMat_c,
self.chi, panel.tvec, tVec_c, self.tvec, panel.distortion)
_, on_panel = panel.clip_to_panel(det_xy, buffer_edges=True)
# all vertices must be on...
patch_is_on = np.all(on_panel.reshape(nangs, 4), axis=1)
patch_xys = det_xy.reshape(nangs, 4, 2)[patch_is_on]
# re-filter...
hkl_ids = hkl_ids[patch_is_on]
hkls_p = hkls_p[patch_is_on, :]
ang_centers = ang_centers[patch_is_on, :]
xy_centers = xy_centers[patch_is_on, :]
ang_pixel_size = ang_pixel_size[patch_is_on, :]
# TODO: add polygon testing right here!
# done <JVB 06/21/16>
if check_only:
patch_output = []
for i_pt, angs in enumerate(ang_centers):
# the evaluation omegas;
# expand about the central value using tol vector
ome_eval = np.degrees(angs[2]) + ome_del
# ...vectorize the omega_to_frame function to avoid loop?
frame_indices = [
ome_imgser.omega_to_frame(ome)[0] for ome in ome_eval
]
if -1 in frame_indices:
if not quiet:
msg = """
window for (%d%d%d) falls outside omega range
""" % tuple(hkls_p[i_pt, :])
print(msg)
continue
else:
these_vertices = patch_xys[i_pt]
ijs = panel.cartToPixel(these_vertices)
ii, jj = polygon(ijs[:, 0], ijs[:, 1])
contains_signal = False
for i_frame in frame_indices:
contains_signal = contains_signal or np.any(
ome_imgser[i_frame][ii, jj] > threshold)
compl.append(contains_signal)
patch_output.append((ii, jj, frame_indices))
else:
# make the tth,eta patches for interpolation
patches = xrdutil.make_reflection_patches(
instr_cfg,
ang_centers[:, :2],
ang_pixel_size,
omega=ang_centers[:, 2],
tth_tol=tth_tol,
eta_tol=eta_tol,
rMat_c=rMat_c,
tVec_c=tVec_c,
distortion=panel.distortion,
npdiv=npdiv,
quiet=True,
beamVec=self.beam_vector)
# GRAND LOOP over reflections for this panel
patch_output = []
for i_pt, patch in enumerate(patches):
# strip relevant objects out of current patch
vtx_angs, vtx_xy, conn, areas, xy_eval, ijs = patch
prows, pcols = areas.shape
nrm_fac = areas / float(native_area)
nrm_fac = nrm_fac / np.min(nrm_fac)
# grab hkl info
hkl = hkls_p[i_pt, :]
hkl_id = hkl_ids[i_pt]
# edge arrays
tth_edges = vtx_angs[0][0, :]
delta_tth = tth_edges[1] - tth_edges[0]
eta_edges = vtx_angs[1][:, 0]
delta_eta = eta_edges[1] - eta_edges[0]
# need to reshape eval pts for interpolation
xy_eval = np.vstack(
[xy_eval[0].flatten(), xy_eval[1].flatten()]).T
# the evaluation omegas;
# expand about the central value using tol vector
ome_eval = np.degrees(ang_centers[i_pt, 2]) + ome_del
# ???: vectorize the omega_to_frame function to avoid loop?
frame_indices = [
ome_imgser.omega_to_frame(ome)[0] for ome in ome_eval
]
if -1 in frame_indices:
if not quiet:
msg = """
window for (%d%d%d) falls outside omega range
""" % tuple(hkl)
print(msg)
continue
else:
# initialize spot data parameters
# !!! maybe change these to nan to not f**k up writer
peak_id = -999
sum_int = None
max_int = None
meas_angs = None
meas_xy = None
# quick check for intensity
contains_signal = False
patch_data_raw = []
for i_frame in frame_indices:
tmp = ome_imgser[i_frame][ijs[0], ijs[1]]
contains_signal = contains_signal or np.any(
tmp > threshold)
patch_data_raw.append(tmp)
pass
patch_data_raw = np.stack(patch_data_raw, axis=0)
compl.append(contains_signal)
if contains_signal:
# initialize patch data array for intensities
if interp.lower() == 'bilinear':
patch_data = np.zeros(
(len(frame_indices), prows, pcols))
for i, i_frame in enumerate(frame_indices):
patch_data[i] = \
panel.interpolate_bilinear(
xy_eval,
ome_imgser[i_frame],
pad_with_nans=False
).reshape(prows, pcols) # * nrm_fac
elif interp.lower() == 'nearest':
patch_data = patch_data_raw # * nrm_fac
else:
msg = "interpolation option " + \
"'%s' not understood"
raise (RuntimeError, msg % interp)
# now have interpolated patch data...
labels, num_peaks = ndimage.label(
patch_data > threshold, structure=label_struct)
slabels = np.arange(1, num_peaks + 1)
if num_peaks > 0:
peak_id = iRefl
coms = np.array(
ndimage.center_of_mass(patch_data,
labels=labels,
index=slabels))
if num_peaks > 1:
center = np.r_[patch_data.shape] * 0.5
center_t = np.tile(center, (num_peaks, 1))
com_diff = coms - center_t
closest_peak_idx = np.argmin(
np.sum(com_diff**2, axis=1))
else:
closest_peak_idx = 0
pass # end multipeak conditional
coms = coms[closest_peak_idx]
# meas_omes = \
# ome_edges[0] + (0.5 + coms[0])*delta_ome
meas_omes = \
ome_eval[0] + coms[0]*delta_ome
meas_angs = np.hstack([
tth_edges[0] + (0.5 + coms[2]) * delta_tth,
eta_edges[0] + (0.5 + coms[1]) * delta_eta,
mapAngle(np.radians(meas_omes), ome_period)
])
# intensities
# - summed is 'integrated' over interpolated
# data
# - max is max of raw input data
sum_int = np.sum(patch_data[
labels == slabels[closest_peak_idx]])
max_int = np.max(patch_data_raw[
labels == slabels[closest_peak_idx]])
# ???: Should this only use labeled pixels?
# Those are segmented from interpolated data,
# not raw; likely ok in most cases.
# need MEASURED xy coords
gvec_c = anglesToGVec(meas_angs,
chi=self.chi,
rMat_c=rMat_c,
bHat_l=self.beam_vector)
rMat_s = makeOscillRotMat(
[self.chi, meas_angs[2]])
meas_xy = gvecToDetectorXY(
gvec_c,
panel.rmat,
rMat_s,
rMat_c,
panel.tvec,
self.tvec,
tVec_c,
beamVec=self.beam_vector)
if panel.distortion is not None:
# FIXME: distortion handling
meas_xy = panel.distortion[0](
np.atleast_2d(meas_xy),
panel.distortion[1],
invert=True).flatten()
pass
# FIXME: why is this suddenly necessary???
meas_xy = meas_xy.squeeze()
pass # end num_peaks > 0
else:
patch_data = patch_data_raw
pass # end contains_signal
# write output
if filename is not None:
if output_format.lower() == 'text':
writer.dump_patch(peak_id, hkl_id, hkl,
sum_int, max_int,
ang_centers[i_pt], meas_angs,
xy_centers[i_pt], meas_xy)
elif output_format.lower() == 'hdf5':
xyc_arr = xy_eval.reshape(prows, pcols,
2).transpose(
2, 0, 1)
writer.dump_patch(
detector_id, iRefl, peak_id, hkl_id,
hkl, tth_edges, eta_edges,
np.radians(ome_eval), xyc_arr, ijs,
frame_indices, patch_data,
ang_centers[i_pt], xy_centers[i_pt],
meas_angs, meas_xy)
pass # end conditional on write output
pass # end conditional on check only
patch_output.append([
peak_id,
hkl_id,
hkl,
sum_int,
max_int,
ang_centers[i_pt],
meas_angs,
meas_xy,
])
iRefl += 1
pass # end patch conditional
pass # end patch loop
output[detector_id] = patch_output
if filename is not None and output_format.lower() == 'text':
writer.close()
pass # end detector loop
if filename is not None and output_format.lower() == 'hdf5':
writer.close()
return compl, output
def peak_finding(self,
im,
transformed,
roi=False,
roi_pos=None,
background_correction=None):
start = time.time()
img = np.copy(im)
if background_correction is None and self.background_correction:
img = self.subtract_background(img)
self.log(self.threshold)
self.log(self.pixel_upper_threshold)
self.log(self.pixel_lower_threshold)
self.log(self.flood_steps)
self.log(img.shape)
self.log(img.max())
img[img < self.threshold] = 0
labels, num_objects = ndi.label(img)
label_size = np.bincount(labels.ravel())
# single photons and no noise
mask_sp = np.where((label_size >= self.pixel_lower_threshold) &
(label_size < self.pixel_upper_threshold), True,
False)
if sum(mask_sp) == 0:
coor_sp = []
else:
label_mask_sp = mask_sp[labels.ravel()].reshape(labels.shape)
labels_sp = label_mask_sp * labels
labels_sp, n_s = ndi.label(labels_sp)
coor_sp = ndi.center_of_mass(img, labels_sp,
range(1,
labels_sp.max() + 1))
# multiple photons
mask_mp = np.where((label_size >= self.pixel_upper_threshold) &
(label_size < np.max(label_size)), True, False)
if sum(mask_mp) > 0:
label_mask_mp = mask_mp[labels.ravel()].reshape(labels.shape)
labels_mp = label_mask_mp * labels
labels_mp, n_m = ndi.label(labels_mp)
for i in range(1, sum(mask_mp) + 1):
objects = ndi.find_objects(labels_mp == i)
if len(objects) == 0:
self.print('No beads found!')
return None
slice_x, slice_y = objects[0]
roi_i = np.copy(img[slice_x, slice_y])
max_i = np.max(roi_i)
step = (0.95 * max_i - self.threshold) / self.flood_steps
multiple = False
coor_tmp = np.array(
ndi.center_of_mass(roi_i,
ndi.label(roi_i)[0]))
for k in range(1, self.flood_steps + 1):
new_threshold = self.threshold + k * step
roi_i[roi_i < new_threshold] = 0
labels_roi, n_i = ndi.label(roi_i)
if n_i > 1:
roi_label_size = np.bincount(labels_roi.ravel())
if np.max(roi_label_size[1:]
) <= self.pixel_upper_threshold:
if len(roi_label_size) == 3 and roi_label_size.min(
) < self.roi_min_size:
break
else:
multiple = True
coordinates_roi = np.array(
ndi.center_of_mass(roi_i, labels_roi,
range(1, n_i + 1)))
[
coor_sp.append(coordinates_roi[j] +
np.array((slice_x.start,
slice_y.start)))
for j in range(len(coordinates_roi))
]
break
if not multiple:
coor_sp.append(coor_tmp +
np.array((slice_x.start, slice_y.start)))
coor = np.array(coor_sp)
if len(coor) == 0:
return None
if roi:
try:
return np.round(coor)[0]
except IndexError:
return None
else:
#peaks_2d = np.round(coor)
peaks_2d = coor
if roi_pos is not None and peaks_2d is not None:
peaks_2d += roi_pos
if self.aligning:
if transformed:
self.tf_peaks_align_ref = np.copy(peaks_2d)
else:
self.peaks_align_ref = np.copy(peaks_2d)
else:
if transformed:
self.tf_peaks = peaks_2d
if self.orig_tf_peaks is None:
self.orig_tf_peaks = np.copy(self.tf_peaks)
else:
self.peaks = np.copy(peaks_2d)
end = time.time()
self.log('duration: ', end - start)
self.print('Number of peaks found: ', peaks_2d.shape[0])
def extract_background_pid(img,
P_id,
slit_height=25,
return_mask=False,
exclude_top_and_bottom=True,
timit=False):
"""
This function marks all relevant pixels for extraction. Extracts the background (ie the inter-order regions = everything outside the order stripes)
from the original 2D spectrum to a sparse matrix containing only relevant pixels.
INPUT:
'img' : 2D echelle spectrum [np.array]
'P_id' : dictionary of the form of {order: np.poly1d} (as returned by make_P_id / identify_stripes)
'slit_height' : half the total slit height in pixels
'return_mask' : boolean - do you want to return the mask of the background locations as well?
'exclude_top_and_bottom' : boolean - do you want to exclude the top and bottom bits (where there are usually incomplete orders)
'timit' : for timing tests...
OUTPUT:
'mat.tocsc()' : scipy.sparse_matrix containing the locations and values of the inter-order regions
'bg_mask' : boolean array containing the location of what is considered background (ie the inter-order space)
"""
if timit:
start_time = time.time()
# logging.info('Extracting background...')
print('Extracting background...')
ny, nx = img.shape
xx = np.arange(nx, dtype='f8')
yy = np.arange(ny, dtype='f8')
x_grid, y_grid = np.meshgrid(xx, yy, copy=False)
bg_mask = np.ones((ny, nx), dtype=bool)
for o, p in sorted(P_id.items()):
# order trace
y = np.poly1d(p)(xx)
# distance from order trace
distance = y_grid - y.repeat(ny).reshape((nx, ny)).T
indices = abs(distance) > slit_height
# include in global mask
bg_mask *= indices
final_bg_mask = bg_mask.copy()
# in case we want to exclude the top and bottom parts where incomplete orders are located
if exclude_top_and_bottom:
print(
'WARNING: this fix works for the current Veloce CCD layout only!!!'
)
labelled_mask, nobj = label(bg_mask)
# WARNING: this fix works for the current Veloce CCD layout only!!!
topleftnumber = labelled_mask[ny - 1, 0]
toprightnumber = labelled_mask[ny - 1, nx - 1]
# bottomleftnumber = labelled_mask[0,0]
bottomrightnumber = labelled_mask[0, nx - 1]
final_bg_mask[labelled_mask == topleftnumber] = False
final_bg_mask[labelled_mask == toprightnumber] = False
final_bg_mask[labelled_mask == bottomrightnumber] = False
mat = sparse.coo_matrix((np.squeeze(np.array(img[final_bg_mask])),
(y_grid[final_bg_mask], x_grid[final_bg_mask])),
shape=(ny, nx))
# return mat.tocsr()
if timit:
print('Elapsed time: ', time.time() - start_time, ' seconds')
if not return_mask:
return mat.tocsc()
else:
return mat.tocsc(), final_bg_mask
