def __init__(self, out_dir, n, spacing_steps = 1, dry = False):
#dry = True
self.dry = dry
self.out_dir = out_dir
if n % 2 != 0:
raise Exception('Center stacking requires even n')
self.n = n
self.n2 = n / 2
# Specify in steps to be very exact instead of units
self.spacing_steps = spacing_steps
if self.dry:
self.mc = DummyController()
self.imager = DummyImager()
else:
self.mc = MC()
self.mc.on()
self.imager = PILImager()
def __init__(self, out_dir, n, spacing_steps=1, dry=False):
#dry = True
self.dry = dry
self.out_dir = out_dir
if n % 2 != 0:
raise Exception('Center stacking requires even n')
self.n = n
self.n2 = n / 2
# Specify in steps to be very exact instead of units
self.spacing_steps = spacing_steps
if self.dry:
self.mc = DummyController()
self.imager = DummyImager()
else:
self.mc = MC()
self.mc.on()
self.imager = PILImager()
class Stacker:
def __init__(self, out_dir, n, spacing_steps=1, dry=False):
#dry = True
self.dry = dry
self.out_dir = out_dir
if n % 2 != 0:
raise Exception('Center stacking requires even n')
self.n = n
self.n2 = n / 2
# Specify in steps to be very exact instead of units
self.spacing_steps = spacing_steps
if self.dry:
self.mc = DummyController()
self.imager = DummyImager()
else:
self.mc = MC()
self.mc.on()
self.imager = PILImager()
def run(self):
if os.path.exists(self.out_dir):
raise Exception("dir %s already exists" % self.out_dir)
os.mkdir(self.out_dir)
self.mc.z.step(-self.n2 * self.spacing_steps)
'''
n = 0: 1 picture
n = 2: 3 pictures
'''
for i in range(self.n + 1):
self.imager.take_picture('%s/%03d__z%04fum.tif' %
(self.out_dir, i, self.mc.z.get_um()))
# Avoid moving at end
if i != self.n:
self.mc.z.step(self.spacing_steps)
time.sleep(3)
print 'Homing...'
self.mc.z.home()
class Stacker:
def __init__(self, out_dir, n, spacing_steps = 1, dry = False):
#dry = True
self.dry = dry
self.out_dir = out_dir
if n % 2 != 0:
raise Exception('Center stacking requires even n')
self.n = n
self.n2 = n / 2
# Specify in steps to be very exact instead of units
self.spacing_steps = spacing_steps
if self.dry:
self.mc = DummyController()
self.imager = DummyImager()
else:
self.mc = MC()
self.mc.on()
self.imager = PILImager()
def run(self):
if os.path.exists(self.out_dir):
raise Exception("dir %s already exists" % self.out_dir)
os.mkdir(self.out_dir)
self.mc.z.step(-self.n2 * self.spacing_steps)
'''
n = 0: 1 picture
n = 2: 3 pictures
'''
for i in range(self.n + 1):
self.imager.take_picture('%s/%03d__z%04fum.tif' % (self.out_dir, i, self.mc.z.get_um()))
# Avoid moving at end
if i != self.n:
self.mc.z.step(self.spacing_steps)
time.sleep(3)
print 'Homing...'
self.mc.z.home()
