def main(args=None):
'''Run CellProfiler
args - command-line arguments, e.g. sys.argv
'''
if args is None:
args = sys.argv
import cellprofiler.preferences as cpprefs
cpprefs.set_awt_headless(True)
exit_code = 0
switches = ('--work-announce', '--knime-bridge-address')
if any(
[any([arg.startswith(switch) for switch in switches])
for arg in args]):
#
# Go headless ASAP
#
cpprefs.set_headless()
for i, arg in enumerate(args):
if arg == "--ij-plugins-directory" and len(args) > i + 1:
cpprefs.set_ij_plugin_directory(args[i + 1])
break
import cellprofiler.worker
cellprofiler.worker.aw_parse_args()
cellprofiler.worker.main()
sys.exit(exit_code)
options, args = parse_args(args)
if options.print_version:
from cellprofiler.utilities.version import \
dotted_version, version_string, git_hash, version_number
print "CellProfiler %s" % dotted_version
print "Git %s" % git_hash
print "Version %s" % version_number
print "Built %s" % version_string.split(" ")[0]
sys.exit(exit_code)
#
# Important to go headless ASAP
#
if (not options.show_gui) or options.write_schema_and_exit:
import cellprofiler.preferences as cpprefs
cpprefs.set_headless()
# What's there to do but run if you're running headless?
# Might want to change later if there's some headless setup
options.run_pipeline = True
if options.jvm_heap_size is not None:
from cellprofiler.preferences import set_jvm_heap_mb
set_jvm_heap_mb(options.jvm_heap_size, False)
set_log_level(options)
if options.print_groups_file is not None:
print_groups(options.print_groups_file)
return
if options.batch_commands_file is not None:
get_batch_commands(options.batch_commands_file)
return
if options.add_message_for_user:
if len(args) != 3:
sys.stderr.write("Usage: (for add_message-for-user)\n")
sys.stderr.write(
"CellProfiler --add-message-for-user <caption> <message> <pipeline-or-project>\n"
)
sys.stderr.write("where:\n")
sys.stderr.write(" <caption> - the message box caption\n")
sys.stderr.write(
" <message> - the message displayed inside the message box\n"
)
sys.stderr.write(
" <pipeline-or-project> - the path to the pipeline or project file to modify\n"
)
return
caption = args[0]
message = args[1]
path = args[2]
import h5py
using_hdf5 = h5py.is_hdf5(path)
if using_hdf5:
import cellprofiler.measurement as cpmeas
m = cpmeas.Measurements(filename=path, mode="r+")
pipeline_text = m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"]
else:
with open(path, "r") as fd:
pipeline_text = fd.read()
header, body = pipeline_text.split("\n\n", 1)
pipeline_text = header + \
("\nMessageForUser:%s|%s\n\n" % (caption, message)) + body
if using_hdf5:
m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"] = pipeline_text
m.close()
else:
with open(path, "w") as fd:
fd.write(pipeline_text)
print "Message added to %s" % path
return
# necessary to prevent matplotlib trying to use Tkinter as its backend.
# has to be done before CellProfilerApp is imported
from matplotlib import use as mpluse
mpluse('WXAgg')
if options.omero_credentials is not None:
set_omero_credentials_from_string(options.omero_credentials)
if options.plugins_directory is not None:
cpprefs.set_plugin_directory(options.plugins_directory, globally=False)
if options.ij_plugins_directory is not None:
cpprefs.set_ij_plugin_directory(options.ij_plugins_directory,
globally=False)
if options.temp_dir is not None:
if not os.path.exists(options.temp_dir):
os.makedirs(options.temp_dir)
cpprefs.set_temporary_directory(options.temp_dir, globally=False)
if not options.allow_schema_write:
cpprefs.set_allow_schema_write(False)
#
# After the crucial preferences are established, we can start the VM
#
from cellprofiler.utilities.cpjvm import cp_start_vm
cp_start_vm()
#
# Not so crucial preferences...
#
if options.image_set_file is not None:
cpprefs.set_image_set_file(options.image_set_file)
try:
# ---------------------------------------
#
# Handle command-line tasks that that need to load the modules to run
#
if options.output_html:
from cellprofiler.gui.html.manual import generate_html
webpage_path = options.output_directory if options.output_directory else None
generate_html(webpage_path)
return
if options.print_measurements:
print_measurements(options)
return
if not hasattr(sys, "frozen") and options.code_statistics:
print_code_statistics()
return
if options.write_schema_and_exit:
write_schema(options.pipeline_filename)
return
#
# ------------------------------------------
if options.show_gui:
import wx
wx.Log.EnableLogging(False)
from cellprofiler.gui.app import App
from cellprofiler.workspace import is_workspace_file
if options.pipeline_filename:
if is_workspace_file(options.pipeline_filename):
workspace_path = os.path.expanduser(
options.pipeline_filename)
pipeline_path = None
else:
pipeline_path = os.path.expanduser(
options.pipeline_filename)
workspace_path = None
elif options.new_project:
workspace_path = False
pipeline_path = None
else:
workspace_path = None
pipeline_path = None
app = App(0,
workspace_path=workspace_path,
pipeline_path=pipeline_path)
if options.data_file is not None:
cpprefs.set_data_file(os.path.abspath(options.data_file))
from cellprofiler.utilities.version import version_string, version_number
logging.root.info("Version: %s / %d" %
(version_string, version_number))
if options.run_pipeline and not options.pipeline_filename:
raise ValueError("You must specify a pipeline filename to run")
if options.output_directory:
if not os.path.exists(options.output_directory):
os.makedirs(options.output_directory)
cpprefs.set_default_output_directory(options.output_directory)
if options.image_directory:
cpprefs.set_default_image_directory(options.image_directory)
if options.show_gui:
if options.run_pipeline:
app.frame.pipeline_controller.do_analyze_images()
app.MainLoop()
return
elif options.run_pipeline:
run_pipeline_headless(options, args)
except Exception, e:
logging.root.fatal("Uncaught exception in CellProfiler.py",
exc_info=True)
exit_code = -1
def main(args):
'''Run CellProfiler
args - command-line arguments, e.g. sys.argv
'''
import cellprofiler.preferences as cpprefs
cpprefs.set_awt_headless(True)
switches = ('--work-announce', '--knime-bridge-address')
if any([any([arg.startswith(switch) for switch in switches])
for arg in args]):
#
# Go headless ASAP
#
cpprefs.set_headless()
for i, arg in enumerate(args):
if arg == "--ij-plugins-directory" and len(args) > i+1:
cpprefs.set_ij_plugin_directory(args[i+1])
break
import cellprofiler.analysis_worker
cellprofiler.analysis_worker.aw_parse_args()
cellprofiler.analysis_worker.main()
sys.exit(0)
if any([arg.startswith('--xml-test-file=') for arg in sys.argv]):
import cpnose
cpnose.main(*sys.argv)
return
options, args = parse_args(args)
if options.print_version:
from cellprofiler.utilities.version import \
dotted_version, version_string, git_hash, version_number
print "CellProfiler %s" % dotted_version
print "Git %s" % git_hash
print "Version %s" % version_number
print "Built %s" % version_string.split(" ")[0]
sys.exit(0)
#
# Important to go headless ASAP
#
if (not options.show_gui) or options.write_schema_and_exit:
import cellprofiler.preferences as cpprefs
cpprefs.set_headless()
# What's there to do but run if you're running headless?
# Might want to change later if there's some headless setup
options.run_pipeline = True
if options.jvm_heap_size is not None:
from cellprofiler.preferences import set_jvm_heap_mb
set_jvm_heap_mb(options.jvm_heap_size, False)
set_log_level(options)
if options.print_groups_file is not None:
print_groups(options.print_groups_file)
return
if options.batch_commands_file is not None:
get_batch_commands(options.batch_commands_file)
return
if options.run_ilastik:
run_ilastik()
return
if options.add_message_for_user:
if len(args) != 3:
sys.stderr.write("Usage: (for add_message-for-user)\n")
sys.stderr.write("CellProfiler --add-message-for-user <caption> <message> <pipeline-or-project>\n")
sys.stderr.write("where:\n")
sys.stderr.write(" <caption> - the message box caption\n")
sys.stderr.write(" <message> - the message displayed inside the message box\n")
sys.stderr.write(" <pipeline-or-project> - the path to the pipeline or project file to modify\n")
return
caption = args[0]
message = args[1]
path = args[2]
import h5py
using_hdf5 = h5py.is_hdf5(path)
if using_hdf5:
import cellprofiler.measurements as cpmeas
m = cpmeas.Measurements(
filename = path, mode="r+")
pipeline_text = m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"]
else:
with open(path, "r") as fd:
pipeline_text = fd.read()
header, body = pipeline_text.split("\n\n", 1)
pipeline_text = header + \
("\nMessageForUser:%s|%s\n\n" % (caption, message)) + body
if using_hdf5:
m[cpmeas.EXPERIMENT, "Pipeline_Pipeline"] = pipeline_text
m.close()
else:
with open(path, "w") as fd:
fd.write(pipeline_text)
print "Message added to %s" % path
return
# necessary to prevent matplotlib trying to use Tkinter as its backend.
# has to be done before CellProfilerApp is imported
from matplotlib import use as mpluse
mpluse('WXAgg')
if (not hasattr(sys, 'frozen')) and options.fetch_external_dependencies:
import external_dependencies
external_dependencies.fetch_external_dependencies(options.overwrite_external_dependencies)
if (not hasattr(sys, 'frozen')) and options.build_extensions:
build_extensions()
if options.build_and_exit:
return
if options.omero_credentials is not None:
set_omero_credentials_from_string(options.omero_credentials)
if options.plugins_directory is not None:
cpprefs.set_plugin_directory(options.plugins_directory,
globally=False)
if options.ij_plugins_directory is not None:
cpprefs.set_ij_plugin_directory(options.ij_plugins_directory,
globally=False)
if options.temp_dir is not None:
if not os.path.exists(options.temp_dir):
os.makedirs(options.temp_dir)
cpprefs.set_temporary_directory(options.temp_dir, globally=False)
if not options.allow_schema_write:
cpprefs.set_allow_schema_write(False)
#
# After the crucial preferences are established, we can start the VM
#
from cellprofiler.utilities.cpjvm import cp_start_vm, cp_stop_vm
cp_start_vm()
#
# Not so crucial preferences...
#
if options.image_set_file is not None:
cpprefs.set_image_set_file(options.image_set_file)
try:
#---------------------------------------
#
# Handle command-line tasks that that need to load the modules to run
#
if options.output_html:
from cellprofiler.gui.html.manual import generate_html
webpage_path = options.output_directory if options.output_directory else None
generate_html(webpage_path)
return
if options.print_measurements:
print_measurements(options)
return
if not hasattr(sys, "frozen") and options.code_statistics:
print_code_statistics()
return
if options.write_schema_and_exit:
write_schema(options.pipeline_filename)
return
#
#------------------------------------------
if options.show_gui:
import wx
wx.Log.EnableLogging(False)
from cellprofiler.cellprofilerapp import CellProfilerApp
from cellprofiler.workspace import is_workspace_file
show_splashbox = (options.pipeline_filename is None and
(not options.new_project) and
options.show_splashbox)
if options.pipeline_filename:
if is_workspace_file(options.pipeline_filename):
workspace_path = os.path.expanduser(options.pipeline_filename)
pipeline_path = None
else:
pipeline_path = os.path.expanduser(options.pipeline_filename)
workspace_path = None
elif options.new_project:
workspace_path = False
pipeline_path = None
else:
workspace_path = None
pipeline_path = None
App = CellProfilerApp(
0,
check_for_new_version = (options.pipeline_filename is None),
show_splashbox = show_splashbox,
workspace_path = workspace_path,
pipeline_path = pipeline_path)
if options.data_file is not None:
cpprefs.set_data_file(os.path.abspath(options.data_file))
from cellprofiler.utilities.version import version_string, version_number
logging.root.info("Version: %s / %d" % (version_string, version_number))
if options.run_pipeline and not options.pipeline_filename:
raise ValueError("You must specify a pipeline filename to run")
if options.output_directory:
if not os.path.exists(options.output_directory):
os.makedirs(options.output_directory)
cpprefs.set_default_output_directory(options.output_directory)
if options.image_directory:
cpprefs.set_default_image_directory(options.image_directory)
if options.show_gui:
if options.run_pipeline:
App.frame.pipeline_controller.do_analyze_images()
App.MainLoop()
return
elif options.run_pipeline:
run_pipeline_headless(options, args)
except Exception, e:
logging.root.fatal("Uncaught exception in CellProfiler.py", exc_info=True)
raise
import logging.config
logging.root.setLevel(logging.INFO)
if len(logging.root.handlers) == 0:
logging.root.addHandler(logging.StreamHandler())
import os
import uuid
import csv
import matplotlib
matplotlib.use('Agg')
import Image
import cellprofiler.preferences as cpprefs
cpprefs.set_headless() # ASAP
cpprefs.set_allow_schema_write(False)
from cellprofiler.pipeline import Pipeline
from cellprofiler.utilities.cpjvm import cp_start_vm, cp_stop_vm
import pydoop.mapreduce.api as api
import pydoop.mapreduce.pipes as pp
from pydoop.avrolib import AvroContext
from pyfeatures.bioimg import BioImgPlane
# basename of the pipeline file, to be uploaded via distributed cache
PIPELINE_BN = 'it.crs4.features.cppipe'
# --- FIXME: the following info should be extracted from the pipeline file
CHANNEL_TAGS = ['D.TIF', 'F.TIF', 'R.TIF']
SET_SIZE = len(CHANNEL_TAGS)
import logging.config
logging.root.setLevel(logging.INFO)
if len(logging.root.handlers) == 0:
logging.root.addHandler(logging.StreamHandler())
import os
import uuid
import csv
import matplotlib
matplotlib.use('Agg')
import Image
import cellprofiler.preferences as cpprefs
cpprefs.set_headless() # ASAP
cpprefs.set_allow_schema_write(False)
from cellprofiler.pipeline import Pipeline
from cellprofiler.utilities.cpjvm import cp_start_vm, cp_stop_vm
import pydoop.mapreduce.api as api
import pydoop.mapreduce.pipes as pp
from pydoop.avrolib import AvroContext
from pyfeatures.bioimg import BioImgPlane
# basename of the pipeline file, to be uploaded via distributed cache
PIPELINE_BN = 'it.crs4.features.cppipe'
# --- FIXME: the following info should be extracted from the pipeline file
CHANNEL_TAGS = ['D.TIF', 'F.TIF', 'R.TIF']
SET_SIZE = len(CHANNEL_TAGS)
