def makeReport() :
#SELECT jc.var_id, COUNT( DISTINCT jc.pat_id )
#FROM `JointCalls` AS jc
#INNER JOIN Variants AS v ON v.id = jc.var_id
#INNER JOIN Isoforms AS i ON i.var_id = v.id
#WHERE AF =0
#AND (
#functionGVS <> 'intron'
#AND functionGVS <> 'near-gene-5'
#AND functionGVS <> 'intergenic'
#AND functionGVS <> 'near-gene-3'
#AND functionGVS <> 'coding-synonymous'
#AND functionGVS <> 'coding-notMod3'
#)
#AND (
#p_AA_AB > .9
#OR p_AA_AB > .9
#OR p_AB_BB > .9
#)
#GROUP BY jc.var_id
#HAVING COUNT( DISTINCT jc.pat_id ) >1
#ORDER BY COUNT( DISTINCT jc.pat_id ) DESC /*inner join Isoforms as i on i.var_id = jc.var_id WHERE p_AA_BB > .90 or p_AA_AB > .9*/
#LIMIT 0 , 30
fout = open("../output/joint.tsv",'w')
csvout = csv.writer( fout, \
delimiter='\t', \
quoting=csv.QUOTE_MINIMAL )
extend = ["patient"] + headers[4:]
reuse = queries.column_headers[:-5]
csvout.writerow( reuse + extend )
conn = db.Conn("localhost")
conn2 = db.Conn("localhost")
call_where = "and (p_AA_AB > .9 or p_AA_AB > .9 or p_AB_BB > .9)"
call_where = ''' and (normal_counts_a / (normal_counts_a + normal_counts_b)
> .95 ) and normal_counts_a + normal_counts_b > 10'''
query = '''
SELECT i.id, %s,%s,%s
FROM `JointCalls` as jc inner join Variants as v on v.id = jc.var_id
inner join Isoforms as i on i.var_id = v.id
inner join Genes as g on i.gene_id = g.id
where AF <= .0005 and
(%s)
%s
and dbSNP is null
and scorePhastCons >= .5
ORDER BY AF, jc.var_id, i.id''' % \
(queries.vcols_string, queries.icols_string, queries.gcols_string, \
queries.gvs, call_where)
rows = conn.query( query )
last_iid = -1
for i, row in enumerate(rows) :
print row
outrow = queries.formatQueryRow(row,offset=1)
iso_id = row[0]
var_id = row[1]
gt_lists = queries.getPatients( conn2, var_id, table='JointCalls', where_clause = call_where )
#print " ----------- "
#print gt_lists
if last_iid != iso_id :
for gt,calls in gt_lists :
for call in calls :
pat_name = call[1]
rest = call[2].split(',')[6:]
together = outrow+[pat_name]+rest
together_string = "".join([str(t) for t in together])
csvout.writerow( together )
last = together_string
last_iid = iso_id
else :
print "dupe!"
#if i > 20 : break
#hets_string = ", ".join([t[1] for t in hets])
#homs_string = ", ".join([t[1] for t in homs])
#if patient in hets_string or patient in homs_string :
#already_called += 1
#outrow.extend( ["-",len(homs),homs_string,len(hets),hets_string] )
fout.close()
from Variants as v inner join Isoforms as i on i.var_id = v.id
inner join Genes as g on g.id = i.gene_id
where chrom = %s and pos = %s and AF < .1 and (%s)''' % \
(queries.vcols_string, queries.icols_string, queries.gcols_string, \
chrom, pos, queries.gvs)
rows = conn.query(query)
num_rows = len(rows)
if num_rows > 0 :
hits += 1
total += 1
for row in rows :
outrow = queries.formatQueryRow(row)
(noinfs, hets, homs) = queries.getPatients( conn2, row[0] )
hets_string = ", ".join([t[1] for t in hets])
homs_string = ", ".join([t[1] for t in homs])
if patient in hets_string or patient in homs_string :
already_called += 1
outrow.extend( ["-",len(homs),homs_string,len(hets),hets_string] )
csvout.writerow( outrow )
# hom and het shares
print patient
print "%d/%d hits/total" % (hits,total)
print "%d/%d already_called/hits" % (already_called,hits)
fin.close()
fout.close()
