def main():
options = mainUsage()
script = options.run
checkDoRun(script)
try:
# Read organism common name and related fasta sequence file
list_file = options.list
util.checkFile(list_file)
for line in open(list_file, "r"):
if line[0] == '!':
continue
if line.count('||') < 1:
continue
# ! common_name||sequence_file
common_name, input_file = util.splitLine(line)
util.checkFile(input_file)
# Run command
cmd = 'python -c "from genepy.annotators.%s import doRun; doRun()" -o %s -i %s' % (script, common_name, input_file)
if util.isLsf():
job_name = "%s.%s" % (common_name, ANNOTATOR_EXTENSION[script])
util.submitJob(job_name, cmd, ANNOTATOR_QUEUE[script])
else:
util.runProcess(cmd)
except Exception, e:
log.error(e)
def fasta2embl(infasta):
"""
Transform sequence file format in fasta to embl using EMBOSS seqret
Returns the name of created embl file
"""
util.checkFile(infasta)
outembl = infasta.split(".")[0] + ".embl"
"""
Usage: seqret
Online documentation: http://emboss.open-bio.org/wiki/Appdoc:Seqret
Standard (Mandatory) qualifiers:
[-sequence] seqall (Gapped) sequence(s) filename and optional
format, or reference (input USA)
[-outseq] seqoutall [<sequence>.<format>] Sequence set(s)
filename and optional format (output USA)
The basic USA syntax is one of:
"file"
"file:entry"
"format::file"
"format::file:entry"
"database:entry"
"database"
"@file"
"""
# Create EMBOSS seqret command line
cmd = "seqret -sequence fasta::%s -outseq embl::%s " % (infasta, outembl)
# Call the subprocess using convenience method
util.runProcess(cmd)
logger.info("File", outembl, "created")
return outembl
def splitSeq(dir, embl, type):
"""
Split sequence into separate file based on CDS features into dir/ directory
based on EMBOSS extractfeat
Usage: extractfeat
Online documentation: http://emboss.open-bio.org/wiki/Appdoc:Extractfeat
Standard (Mandatory) qualifiers:
[-sequence] seqall Sequence(s) filename and optional format, or
reference (input USA)
[-outseq] seqout [.] Sequence filename and
optional format (output USA)
Additional (Optional) qualifiers:
-type string [*] By default every feature in the feature
table is extracted. You can set this to be
any feature type you wish to extract.
See http://www.ebi.ac.uk/Services/WebFeat/
for a list of the EMBL feature types and see
the Uniprot user manual in
http://www.uniprot.org/manual/sequence_annotation
for a list of the Uniprot feature types.
The type may be wildcarded by using '*'.
If you wish to extract more than one type,
separate their names with the character '|',
eg:
*UTR | intron (Any string is accepted)
-featinname boolean [N] To aid you in identifying the type of
feature that has been output, the type of
feature is added to the start of the
description of the output sequence.
Sometimes the description of a sequence is
lost in subsequent processing of the
sequences file, so it is useful for the type
to be a part of the sequence ID name. If
you set this to be TRUE then the name is
added to the ID name of the output sequence.
Associated qualifiers:
"-outseq" associated qualifiers
-ossingle2 boolean Separate file for each entry
-ofdirectory2 string Output directory
The basic USA syntax is one of:
"file"
"file:entry"
"format::file"
"format::file:entry"
"database:entry"
"database"
"@file"
"""
util.checkFile(embl)
# Create directory
util.createDir(dir)
cmd = "extractfeat -sequence embl::%s -type %s -featinname YES -outseq fasta:: -osextension2 ffn -ossingle2 Yes -osdirectory2 %s" % (embl, type, dir)
util.runProcess(cmd)
logger.info("Sequences extracted into %s" % dir)
def concatSeq(genome_file, dir):
"""
Concatenate separated CDS sequence fasta files located in dir into one file
"""
util.checkDir(dir)
if os.path.exists(genome_file):
os.remove(genome_file)
cmd = "cat %s/*.faa > %s" % (dir, genome_file)
util.runProcess(cmd)
logger.info("concatSeq finished")
def runFasta(seq_dir, genomes_dir, fasta_dir):
"""
Run FASTA on protein sequences between new genome against all in house genomes
FASTA searches a protein or DNA sequence data bank
version 35.04 Aug. 25, 2009
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
"""
util.createDir(fasta_dir)
# List of in-house genomes
util.checkDir(genomes_dir)
genome_files = []
logger.info("Create fasta results directory for each in-house reference genome")
for genome_file in os.listdir(genomes_dir):
if '.faa' in genome_file:
genome_files.append(genome_file)
# Create fasta results directory for each in-house genome
util.createDir("%s/%s" % (fasta_dir, genome_file.split(".")[0]))
logger.info(genome_file)
util.checkDir(seq_dir)
if IS_LSF:
# Rename new genome sequences for job array to be mygenome_1.faa mygenome_2.faa ...
seq_num = 0
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
seq_num += 1
if 'mygenome_' in seq_file and '.faa' in seq_file:
continue
seq_newfilepath = "%s/mygenome_%s.faa" % (seq_dir, seq_num)
seq_filepath = "%s/%s" % (seq_dir, seq_file)
os.rename(seq_filepath, seq_newfilepath)
# Submit bsub job array on mygenome_${LSB_JOBINDEX}.faa against one refgenome at a time
bsub_dir = "bsub"
util.checkDir(bsub_dir)
for genome_file in genome_files:
res_dir = "%s/%s" % (fasta_dir, genome_file.split(".")[0])
cmd = "fasta35 -z 1 -Q -H -S -m 10 %s/mygenome_${LSB_JOBINDEX}.faa %s/%s > %s/mygenome_${LSB_JOBINDEX}.fa" % (seq_dir, genomes_dir, genome_file, res_dir)
util.submitJobArray(jobname="genepy-fasta", jobnum=seq_num, jobdir=bsub_dir, cmd=cmd)
util.submitJobDependency('genepy-fasta')
logger.info("Fasta on LSF finished")
else:
# List of new genome sequences
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
res_file = seq_file.split(".")[0] + ".fa"
for genome_file in genome_files:
res_dir = "%s/%s" % (fasta_dir, genome_file.split(".")[0])
cmd = "fasta35 -z 1 -Q -H -S -m 10 %s/%s %s/%s > %s/%s" % (seq_dir, seq_file, genomes_dir, genome_file, res_dir, res_file)
util.runProcess(cmd)
logger.info(seq_file)
logger.info("Fasta finished")
def translateSeq(dir):
"""
Translate nucleic acid sequence in fasta format into protein sequence using
EMBOSS transeq
Usage: transeq
Online documentation: http://emboss.open-bio.org/wiki/Appdoc:Transeq
Standard (Mandatory) qualifiers:
[-sequence] seqall Nucleotide sequence(s) filename and optional
format, or reference (input USA)
[-outseq] seqoutall [.] Protein sequence
set(s) filename and optional format (output
USA)
Additional (Optional) qualifiers:
-table menu [0] Code to use (Values: 0 (Standard); 1
(Standard (with alternative initiation
codons)); 2 (Vertebrate Mitochondrial); 3
(Yeast Mitochondrial); 4 (Mold, Protozoan,
Coelenterate Mitochondrial and
Mycoplasma/Spiroplasma); 5 (Invertebrate
Mitochondrial); 6 (Ciliate Macronuclear and
Dasycladacean); 9 (Echinoderm
Mitochondrial); 10 (Euplotid Nuclear); 11
(Bacterial); 12 (Alternative Yeast Nuclear);
13 (Ascidian Mitochondrial); 14 (Flatworm
Mitochondrial); 15 (Blepharisma
Macronuclear); 16 (Chlorophycean
Mitochondrial); 21 (Trematode
Mitochondrial); 22 (Scenedesmus obliquus);
23 (Thraustochytrium Mitochondrial))
The basic USA syntax is one of:
"file"
"file:entry"
"format::file"
"format::file:entry"
"database:entry"
"database"
"@file"
"""
util.checkDir(dir)
for file in os.listdir(dir):
if '.ffn' in file:
infasta = file
outpep = file.split(".")[0] + ".faa"
cmd = "transeq -sequence fasta::%s/%s -outseq fasta::%s/%s -table 11" % (dir, infasta, dir, outpep)
util.runProcess(cmd)
logger.info("Sequences translated.")
def concatFeatures(embl, features):
"""
Concat CDS features in embl format into embl sequence file
- the first two lines of embl sequence containing ID & XX lines
- the CDS features file containing FT lines
- the rest of embl sequence containing SQ lines
Returns the name of created embl sequence file
"""
util.checkFile(embl)
util.checkFile(features)
outembl = embl.split(".")[0] + "_with_cds.embl"
# Create command line
head_cmd = "head -2 %s > %s; cat %s >> %s;" % (embl, outembl, features, outembl)
util.runProcess(head_cmd)
tail_cmd = "tail +3 %s > tail; cat tail >> %s; rm tail;" % (embl, outembl)
util.runProcess(tail_cmd)
logger.info("File", outembl, "created")
return outembl
def runReciprocalFasta(seq_dir, genome_file, fasta_dir):
"""
Run FASTA between extracted in-house protein sequences against new genome
FASTA searches a protein or DNA sequence data bank
version 35.04 Aug. 25, 2009
W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448
"""
util.createDir(fasta_dir)
# Check new genome
util.checkFile(genome_file)
# Check ref genome extracted sequences
util.checkDir(seq_dir)
res_dir = fasta_dir
if IS_LSF:
# Rename new genome sequences for job array to be refgenome_1.faa refgenome_2.faa ...
seq_num = 0
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
seq_num += 1
if 'refgenome_' in seq_file and '.faa' in seq_file:
continue
seq_newfilepath = "%s/refgenome_%s.faa" % (seq_dir, seq_num)
seq_filepath = "%s/%s" % (seq_dir, seq_file)
os.rename(seq_filepath, seq_newfilepath)
# Submit bsub job array on refgenome_${LSB_JOBINDEX}.faa against mygenome
bsub_dir = "bsub"
util.checkDir(bsub_dir)
cmd = "fasta35 -z 1 -Q -H -S -m 10 %s/refgenome_${LSB_JOBINDEX}.faa %s > %s/refgenome_${LSB_JOBINDEX}.fa" % (seq_dir, genome_file, res_dir)
util.submitJobArray(jobname="genepy-recipfasta", jobnum=seq_num, jobdir=bsub_dir, cmd=cmd)
util.submitJobDependency('genepy-recipfasta')
logger.info("Reciprocal Fasta on LSF finished")
else:
# List of inhouse extracted genome sequences
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
res_file = seq_file.split(".")[0] + ".fa"
cmd = "fasta35 -z 1 -Q -H -S -m 10 %s/%s %s > %s/%s" % (seq_dir, seq_file, genome_file, res_dir, res_file)
util.runProcess(cmd)
logger.info(seq_file)
logger.info("Reciprocal Fasta finished")
def runHamapScan(seq_dir, hamap_dir):
"""
HAMAP: High-quality Automated and Manual Annotation of microbial Proteomes
ftp download site: ftp://ftp.expasy.org/databases/hamap/
pfscan compares a protein or nucleic acid sequence against a profile
library. The result is an unsorted list of profile-sequence matches.
download site: http://www.isrec.isb-sib.ch/ftp-server/pftools/pft2.3/
"""
util.createDir(hamap_dir)
util.checkDir(seq_dir)
hamap_profile_file = "%s/hamap/hamap.prf" % os.path.dirname(__file__)
if IS_LSF:
# Rename new genome sequences for job array to be mygenome_1.faa mygenome_2.faa ...
seq_num = 0
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
seq_num += 1
if 'mygenome_' in seq_file and '.faa' in seq_file:
continue
seq_newfilepath = "%s/mygenome_%s.faa" % (seq_dir, seq_num)
seq_filepath = "%s/%s" % (seq_dir, seq_file)
os.rename(seq_filepath, seq_newfilepath)
# Submit bsub job array on mygenome_${LSB_JOBINDEX}.faa against hamap profile
bsub_dir = "bsub"
util.checkDir(bsub_dir)
cmd = "pfscan -klf %s/mygenome_${LSB_JOBINDEX}.faa %s > %s/mygenome_${LSB_JOBINDEX}.out" % (seq_dir, hamap_profile_file, hamap_dir)
util.submitJobArray(jobname='genepy-hamap', jobnum=seq_num, jobdir=bsub_dir, cmd=cmd)
util.submitJobDependency('genepy-hamap')
logger.info("HAMAP scan on LSF finished")
else:
# List of new genome sequences
for seq_file in os.listdir(seq_dir):
if not '.faa' in seq_file:
continue
res_file = seq_file.split(".")[0] + ".out"
cmd = "pfscan -klf %s/%s %s > %s/%s" % (seq_dir, seq_file, hamap_profile_file, hamap_dir, res_file)
util.runProcess(cmd)
logger.info("HAMAP scan finished")
