Image correction software for chromatic shifts in fluorescence microscopy.

• Most microscopy formats

• multipage-tiff (imageJ format or ome.tif format)
• DeltaVision (.dv)

Read the domument

The ready to use packages are available here

The source codes are written in pure python and works on the following platforms and dependencies.

Platforms successfully tested:

• MacOSX64bit (10.9.5 or higher)
• Windows64bit (XP or higher)
• Linux Ubuntu(16), CentOS(6)

Dependencies:

• python (v2.7 or v3.6)
• numpy
• scipy
• wxPython (v3 or v4)
• PyOpenGL
• tifffile (v0.14.0)
• pyFFTW (optional for fast Fourier transform)
• javabridge (optional for reading more image format)
• python-bioformats (optional for reading more image format)
• lxml (optional for reading more image format)
• Java Development Kit (optional for reading more image format)

Please cite our paper:

Matsuda, Schermelleh, Hirano, Haraguchi, Hiraoka, 2018 "Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy" Sci Rep 8:7583

Authored and maintained by Atsushi Matsuda.