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Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching

The work is published in here.

Basically, there are three parts of the work:

  • E-coli genomic DNA sqeuencing comparison (TGIRT vs NexteraXT [Illumina Basespace Project 21071065])
    • codes in genome_tgirt_seq
  • Plasma cell-free DNA-seq (TGIRT vs ssDNA-seq)
    • codes in plasmadna-tgirt
  • Bisulfite sequencing of plasma cfDNA
    • codes in bisufile-seq

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