pVAC-Seq is a cancer immunotherapy pipeline for the identification of personalized Variant Antigens by Cancer Sequencing (pVAC-Seq) that integrates tumor mutation and expression data (DNA- and RNA-Seq). It enables cancer immunotherapy research by using massively parallel sequence data to predicting tumor-specific mutant peptides (neoantigens) that can elicit anti-tumor T cell immunity. It is being used in studies of checkpoint therapy response and to identify targets for cancer vaccines and adoptive T cell therapies. For more general information, see the manuscript published in Genome Medicine.

Jasreet Hundal, Beatriz M. Carreno, Allegra A. Petti, Gerald P. Linette, Obi L. Griffith, Elaine R. Mardis, and Malachi Griffith. pVAC-Seq: A genome-guided in silico approach to identifying tumor neoantigens. Genome Medicine. 2016, 8:11, DOI: 10.1186/s13073-016-0264-5. PMID: 26825632.

This project is licensed under NPOSL-3.0.

pVAC-Seq requires Python 3.5. Before running any installation steps check the Python version installed on your system:

python -V

If you don’t have Python 3.5 installed, we recommend using Conda to emulate a Python 3.5. environment. We’ve encountered problems with users that already have Python 2.x installed when they also try to install Python 3.5. The defaults will not be set correctly in that case. If you already have Python 2.x installed we strongly recommmend using Conda instead of installing Python 3.5 locally.

Once you have set up your Python 3.5 environment correctly you can use pip to install pVAC-Seq. Make sure you have pip installed. pip is generally included in python distributions, but may need to be upgraded before use. See the instructions for installing or upgrading pip.

After you have pip installed, type the following command on your Terminal (for Mac and Linux users) or the Command Prompt (for Windows users):

pip install pvacseq

You can check that pvacseq has been installed under the default environment by listing all installed packages:

pip list

pip will fetch and install pVAC-Seq and its dependencies for you. After installing, you can run pvacseq directly from the Terminal/Command Prompt.

If you have an old version of pVAC-Seq installed you might want to consider upgrading to the latest version:

pip install pvacseq --upgrade

If you would like to run pVAC-Seq with a user interface and built-in data visualizations, install pVAC-Seq with the API extra:

pip install pvacseq[API] --upgrade

Then you can start the pVAC-Seq server by running the command:

pvacseq-api

Then navigate to localhost:8000 in your web browser to manage pVAC-Seq.

• Port 8080 for operating the internal api
• Port 8000 for serving the web client to your browser
• Port 5006 for rendering data visualizations

The pVAC-Seq documentation can be found on ReadTheDocs.