def readFramesFromBuffer(self, img_bg=None):
sys.path.append(mm_directory)
import MMCorePy
md = MMCorePy.Metadata()
elapsed_frame_time = []
frame_list = np.zeros(
(self.getBufferSizeFrames(), self.getImageHeight(),
self.getImageWidth()),
dtype=np.uint16)
for img_idx in range(frame_list.shape[0]):
if img_bg is not None:
frame_list[img_idx, :, :] = np.max(
self.mmc.popNextImageMD(md).copy().astype(np.int) -
img_bg).astype(np.uint16)
else:
frame_list[img_idx, :, :] = self.mmc.popNextImageMD(md).copy()
elapsed_frame_time.append(
float(md.GetSingleTag("ElapsedTime-ms").GetValue()))
elapsed_frame_time = np.asarray(elapsed_frame_time)
elapsed_frame_time = elapsed_frame_time - np.min(elapsed_frame_time)
return (frame_list, elapsed_frame_time.tolist())
# GetImageBufferSize() = img_.Width() * img_.Height() * GetImageBytesPerPixel()
print('getBytesPerPixel: %s') % mmc.getBytesPerPixel()
print('getImageBitDepth: %s') % mmc.getImageBitDepth()
print("""
* Acquisition test *
""")
mmc.startContinuousSequenceAcquisition(1)
# Видимо буфер инициализируется только при захвате.
print('getBufferTotalCapacity: %s') % mmc.getBufferTotalCapacity()
print('getBufferFreeCapacity: %s') % mmc.getBufferFreeCapacity()
print('getExposure %s') % mmc.getExposure()
mmc.setProperty(devlabel, 'Exposure', 15)
print('getExposure %s') % mmc.getExposure()
print("""
* Frame metadata *
""")
md = MMCorePy.Metadata()
img = mmc.getLastImageMD(0, 0, md)
# print(img.shape)
print(md.Dump())
print("""
* Reset all *
""")
mmc.stopSequenceAcquisition()
mmc.reset()