def createOptionsFrame(self): """ Creates a frame that contains the various widgets used to control the colocalization settings """ #TaskPanel.createOptionsFrame(self) # self.colocalizationPanel=wx.Panel(self.settingsNotebook,-1) self.colocalizationPanel = wx.Panel(self, -1) self.colocalizationSizer = wx.GridBagSizer() n = 0 self.lowerThresholdLbl1 = wx.StaticText(self.colocalizationPanel, -1, "Ch1 lower threshold:") self.upperThresholdLbl1 = wx.StaticText(self.colocalizationPanel, -1, "Ch1 upper threshold:") self.lowerThresholdLbl2 = wx.StaticText(self.colocalizationPanel, -1, "Ch2 lower threshold:") self.upperThresholdLbl2 = wx.StaticText(self.colocalizationPanel, -1, "Ch2 upper threshold:") self.lowerthreshold1 = wx.Slider(self.colocalizationPanel, value = 128, minValue = 1, \ maxValue = 255, size = (300, -1), style = wx.SL_HORIZONTAL | wx.SL_LABELS) self.lowerthreshold1.Bind(wx.EVT_SCROLL, self.updateThreshold) self.upperthreshold1 = wx.Slider(self.colocalizationPanel, value = 128, minValue = 1, \ maxValue = 255, size = (300, -1), style = wx.SL_HORIZONTAL | wx.SL_LABELS) self.upperthreshold1.Bind(wx.EVT_SCROLL, self.updateThreshold) self.lowerthreshold2 = wx.Slider(self.colocalizationPanel, value = 128, minValue = 1, \ maxValue = 255, size = (300, -1), style = wx.SL_HORIZONTAL | wx.SL_LABELS) self.lowerthreshold2.Bind(wx.EVT_SCROLL, self.updateThreshold) self.upperthreshold2 = wx.Slider(self.colocalizationPanel, value = 128, minValue = 1, \ maxValue = 255, size = (300, -1), style = wx.SL_HORIZONTAL | wx.SL_LABELS) self.upperthreshold2.Bind(wx.EVT_SCROLL, self.updateThreshold) self.colocalizationSizer.Add(self.lowerThresholdLbl1, (n, 0)) n += 1 self.colocalizationSizer.Add(self.lowerthreshold1, (n, 0)) n += 1 self.colocalizationSizer.Add(self.upperThresholdLbl1, (n, 0)) n += 1 self.colocalizationSizer.Add(self.upperthreshold1, (n, 0)) n += 1 self.colocalizationSizer.Add(self.lowerThresholdLbl2, (n, 0)) n += 1 self.colocalizationSizer.Add(self.lowerthreshold2, (n, 0)) n += 1 self.colocalizationSizer.Add(self.upperThresholdLbl2, (n, 0)) n += 1 self.colocalizationSizer.Add(self.upperthreshold2, (n, 0)) n += 1 sbox = wx.StaticBox(self.colocalizationPanel, -1, "2D histogram (right-click for more options)") box = wx.StaticBoxSizer(sbox, wx.VERTICAL) self.scatterPlot = Scatterplot(self.colocalizationPanel, drawLegend = 1) lib.messenger.connect(self.scatterPlot, "scatterplot_thresholds", self.onUpdateScatterplotThresholds) box.Add(self.scatterPlot) self.colocalizationSizer.Add(box, (n, 0)) n += 1 sbox = wx.StaticBox(self.colocalizationPanel, -1, "Colocalization quantification") box = wx.StaticBoxSizer(sbox, wx.VERTICAL) self.automaticThresholdStaticBox = sbox self.automaticThresholdSizer = box #self.pvaluebox = wx.StaticBox(self.colocalizationPanel, -1, "Calculate P-Value") self.radiobox = wx.RadioBox(self.colocalizationPanel, -1, "Calculate P-Value", choices = ["None", "Costes", "Fay", "van Steensel"], majorDimension = 2, style = wx.RA_SPECIFY_COLS ) self.radiobox.Bind(wx.EVT_RADIOBOX, self.onSetTestMethod) #self.pvalueNone = wx.RadioButton(self.colocalizationPanel, -1, "None", name="pvalue") #self.pvalueNone.SetValue(True) #self.pvalueNone.Bind(wx.EVT_RADIOBUTTON, self.onSetNone) #self.pvalueCostes = wx.RadioButton(self.colocalizationPanel, -1, "Costes", name="pvalue") #self.pvalueCostes.SetValue(False) #self.pvalueCostes #self.pvalueFay = wx.RadioButton(self.colocalizationPanel, -1, "Fay", name="pvalue") #self.pvalueFay.SetValue(False) #self.pvaluevanSteensel = wx.RadioButton(self.colocalizationPanel, -1, "van Steensel", name="pvalue") #self.pvaluevanSteensel.SetValue(False) box.Add(self.radiobox) self.iterLbl = wx.StaticText(self.colocalizationPanel, -1, "Iterations:") self.iterations = wx.SpinCtrl(self.colocalizationPanel, -1, "100", min = 2, \ max = 999, initial = 100) self.iterations.Bind(wx.EVT_SPINCTRL, self.onSetIterations) fixedPSFLbl = wx.StaticText(self.colocalizationPanel, -1, "PSF radius (px):") self.fixedPSF = wx.TextCtrl(self.colocalizationPanel, -1, "") costesgrid = wx.GridBagSizer() #sboxsizer.Add(costesgrid) costesgrid.Add(self.iterLbl, (0, 0)) costesgrid.Add(self.iterations, (0, 1)) costesgrid.Add(fixedPSFLbl, (1, 0)) costesgrid.Add(self.fixedPSF, (1, 1)) NALbl = wx.StaticText(self.colocalizationPanel, -1, "Numerical Aperture:") self.NA = wx.TextCtrl(self.colocalizationPanel, -1, "1.4") costesgrid.Add(NALbl, (2, 0)) costesgrid.Add(self.NA, (2, 1)) Ch2LambdaLbl = wx.StaticText(self.colocalizationPanel, -1, u"Ch2 \u03BB (nm):") self.Ch2Lambda = wx.TextCtrl(self.colocalizationPanel, -1, "520") costesgrid.Add(Ch2LambdaLbl, (3, 0)) costesgrid.Add(self.Ch2Lambda, (3, 1)) #pvaluebox.Add(costesgrid) self.NA.Bind(wx.EVT_TEXT, self.onUpdatePSF) self.Ch2Lambda.Bind(wx.EVT_TEXT, self.onUpdatePSF) self.iterations.Enable(0) self.NA.Enable(0) self.Ch2Lambda.Enable(0) self.fixedPSF.Enable(0) box.Add(costesgrid) box2 = wx.BoxSizer(wx.HORIZONTAL) self.statsButton = wx.Button(self.colocalizationPanel, -1, "Calculate statistics") self.statsButton.Bind(wx.EVT_BUTTON, self.getStatistics) box2.Add(self.statsButton) self.thresholdButton = wx.Button(self.colocalizationPanel, -1, "Calculate thresholds") self.thresholdButton.Bind(wx.EVT_BUTTON, self.getAutoThreshold) box2.Add(self.thresholdButton) self.statsButtonSizer = box2 box.Add(box2) self.colocalizationSizer.Add(box, (n, 0), flag = wx.EXPAND | wx.LEFT | wx.RIGHT) n += 1 sbox = wx.StaticBox(self.colocalizationPanel, -1, "Coloc Volume Statistics") box = wx.StaticBoxSizer(sbox, wx.VERTICAL) self.colocStatsStaticBox = sbox self.colocStatsSizer = box self.listctrl = GUI.ColocListView.ColocListView(self.colocalizationPanel, -1, size = (350, 300)) box.Add(self.listctrl) self.statsButton = wx.Button(self.colocalizationPanel, -1, "Export") self.statsButton.Bind(wx.EVT_BUTTON, self.onExportStatistics) box.Add(self.statsButton) self.listctrl.updateListCtrl(self.getVariables()) self.colocalizationSizer.Add(box, (n, 0)) n += 1 sbox = wx.StaticBox(self.colocalizationPanel, -1, "Colocalization color") sboxsizer = wx.StaticBoxSizer(sbox, wx.VERTICAL) self.colocColorBox = sbox self.colocColorSizer = sboxsizer self.colorBtn = CTFButton(self.colocalizationPanel) sboxsizer.Add(self.colorBtn) val = AcceptedValidator self.constColocCheckbox = wx.CheckBox(self.colocalizationPanel, -1, \ "Use single (1-bit) value for colocalization") self.constColocValue = wx.TextCtrl(self.colocalizationPanel, -1, "255", validator = val(string.digits, below = 255)) self.constColocValue.Enable(0) self.constColocValue.Bind(wx.EVT_TEXT, self.onSetColocValue) self.constColocCheckbox.Bind(wx.EVT_CHECKBOX, self.onSetColocCheckbox) sboxsizer.Add(self.constColocCheckbox) sboxsizer.Add(self.constColocValue) self.colocalizationSizer.Add(sboxsizer, (n, 0)) n += 1 self.colocalizationPanel.SetSizer(self.colocalizationSizer) self.colocalizationPanel.SetAutoLayout(1) self.settingsSizer.Add(self.colocalizationPanel, (1, 0), flag = wx.EXPAND | wx.ALL)
