def WillItPCR(self, amplicon, f_primer, r_primer):
for_wip = self.SWTm_WillItPCR_primer(amplicon, MB.Antisense(f_primer))
rev_wip = self.SWTm_WillItPCR_primer(amplicon, r_primer)
ret = {}
if for_wip['TmAB'] > 0:
ret['for_Tm'] = for_wip['TmAB']
ret['for_align_score'] = for_wip['align_score']
ret['for_match'] = for_wip['match_str']
ret['for_mis_matches'] = for_wip['mis_matches']
if rev_wip['TmAB'] > 0:
ret['rev_Tm'] = rev_wip['TmAB']
ret['rev_align_score'] = rev_wip['align_score']
ret['rev_match'] = rev_wip['match_str']
ret['rev_mis_matches'] = rev_wip['mis_matches']
return ret
def Antisense(self):
self.label = self.label + '|as'
self.seq = MB.Antisense(self.seq)