Beispiel #1
0
    def format_protein_db(self, input_file_path, output_file_path):
        progress.new('Formatting raw files')
        progress.update('Decompressing protein sequences')

        # poor man's uncompress
        temp_fasta_path = filesnpaths.get_temp_file_path()
        with open(temp_fasta_path,
                  'wb') as f_out, gzip.open(input_file_path, 'rb') as f_in:
            f_out.write(f_in.read())

        progress.end()

        if utils.is_program_exists('diamond', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_DIAMOND')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('Diamond log', log_file_path)

            diamond = Diamond(temp_fasta_path)
            diamond.num_threads = self.num_threads
            diamond.run.log_file_path = log_file_path
            diamond.makedb(output_db_path)
        else:
            self.run.warning(
                "Diamond does not seem to be installed on this system, so anvi'o is not going to\
                              generate a search database for it. Remember this when/if things go South."
            )

        if utils.is_program_exists(
                'makeblastdb', dont_raise=True) and utils.is_program_exists(
                    'blastp', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_BLAST')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('BLAST log', log_file_path)

            blast = BLAST(temp_fasta_path)
            blast.run.log_file_path = log_file_path
            blast.num_threads = self.num_threads
            blast.makedb(os.path.join(output_db_path, 'COG.fa'))
        else:
            self.run.warning(
                "BLAST tools do not seem to be installed on this system, so anvi'o is not going to\
                              generate a search database for them to be used. Keep this in mind for later."
            )

        os.remove(temp_fasta_path)
Beispiel #2
0
    def format_protein_db(self, input_file_path, output_file_path):
        progress.new('Formatting raw files')
        progress.update('Decompressing protein sequences')

        # poor man's uncompress
        temp_fasta_path = filesnpaths.get_temp_file_path()
        try:
            with open(temp_fasta_path, 'wb') as f_out, gzip.open(input_file_path, 'rb') as f_in:
                f_out.write(f_in.read())
        except Exception as e:
            progress.end()
            raise ConfigError(f"Something went wrong while decompressing the downloaded file :/ It is likely that "
                              f"the download failed and only part of the file was downloaded. If you would like to "
                              f"try again, please run the setup command with the flag `--reset`. Here is what the "
                              f"downstream library said: '{e}'.")

        progress.end()

        if utils.is_program_exists('diamond', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_DIAMOND')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('Diamond log', log_file_path)

            diamond = Diamond(temp_fasta_path)
            diamond.num_threads = self.num_threads
            diamond.run.log_file_path = log_file_path
            diamond.makedb(output_db_path)
        else:
            self.run.warning("DIAMOND does not seem to be installed on this system, so anvi'o is not going to "
                             "generate a search database for it. Remember this when/if things go South.")

        if utils.is_program_exists('makeblastdb', dont_raise=True) and utils.is_program_exists('blastp', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_BLAST')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('BLAST log', log_file_path)

            blast = BLAST(temp_fasta_path)
            blast.run.log_file_path = log_file_path
            blast.num_threads = self.num_threads
            blast.makedb(os.path.join(output_db_path, 'COG.fa'))
        else:
            self.run.warning("BLAST tools do not seem to be installed on this system, so anvi'o is not going to "
                             "generate a search database for them to be used. Keep this in mind for later.")

        os.remove(temp_fasta_path)
Beispiel #3
0
    def format_protein_db(self, input_file_path, output_file_path):
        progress.new('Formatting raw files')
        progress.update('Decompressing protein sequences')

        # poor man's uncompress
        temp_fasta_path = filesnpaths.get_temp_file_path()
        with open(temp_fasta_path, 'wb') as f_out, gzip.open(input_file_path, 'rb') as f_in:
            f_out.write(f_in.read())

        progress.end()

        if utils.is_program_exists('diamond', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_DIAMOND')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('Diamond log', log_file_path)

            diamond = Diamond(temp_fasta_path)
            diamond.num_threads = self.num_threads
            diamond.run.log_file_path = log_file_path
            diamond.makedb(output_db_path)
        else:
            self.run.warning("Diamond does not seem to be installed on this system, so anvi'o is not going to\
                              generate a search database for it. Remember this when/if things go South.")

        if utils.is_program_exists('makeblastdb', dont_raise=True) and utils.is_program_exists('blastp', dont_raise=True):
            output_dir = J(self.COG_data_dir, 'DB_BLAST')
            if os.path.exists(output_dir):
                shutil.rmtree(output_dir)

            os.mkdir(output_dir)

            output_db_path = J(output_dir, 'COG')
            log_file_path = J(output_dir, 'log.txt')

            self.run.info('BLAST log', log_file_path)

            blast = BLAST(temp_fasta_path)
            blast.run.log_file_path = log_file_path
            blast.num_threads = self.num_threads
            blast.makedb(os.path.join(output_db_path, 'COG.fa'))
        else:
            self.run.warning("BLAST tools do not seem to be installed on this system, so anvi'o is not going to\
                              generate a search database for them to be used. Keep this in mind for later.")

        os.remove(temp_fasta_path)