def main():
with TkinterDialog(workingDirectory = getDataDirectory()) as dialog:
dialog.createMultipleFileSelector("Sam Files:", 0, ".sam", ("Sam Files",(".sam.gz",".sam")),
additionalFileEndings = ".sam.gz")
trimmedFastqToSam(dialog.selections.getFilePathGroups()[0])
def main():
# Create the Tkinter dialog.
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector(
"Bed Mutation File:", 0, "singlenuc_" + DataTypeStr.mutations + ".bed",
("Bed Files", ".bed"))
dialog.createDropdown("Expansion Context", 1, 0,
("Trinuc/Quadrunuc", "Pentanuc/Hexanuc"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
inputBedFilePaths = list(selections.getFilePathGroups())[
0] # A list of paths to original bed mutation files
expansionContext = list(selections.getDropdownSelections())[
0] # What context the file should be expanded to.
if expansionContext == "Trinuc/Quadrunuc":
expansionContextNum = 3
elif expansionContext == "Pentanuc/Hexanuc":
expansionContextNum = 5
else:
raise ValueError("Matching strings is hard.")
expandContext(inputBedFilePaths, expansionContextNum)
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Custom bed Input Files:", 0,
"custom_input.bed",
("bed files", ".bed"))
dialog.createFileSelector("Genome Fasta File:", 1, ("Fasta Files", ".fa"))
dialog.createCheckbox("Stratify data by microsatellite stability?", 2, 0)
dialog.createCheckbox("Stratify by mutation signature?", 2, 1)
dialog.createCheckbox("Separate individual cohorts?", 3, 0)
dialog.createCheckbox("Only use single nucleotide substitutions?", 4, 0)
dialog.createCheckbox("Include indels in output?", 4, 1)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
bedInputFilePaths = selections.getFilePathGroups()[0]
genomeFilePath = selections.getIndividualFilePaths()[0]
stratifyByMS = selections.getToggleStates()[0]
stratifyByMutSig = selections.getToggleStates()[1]
separateIndividualCohorts = selections.getToggleStates()[2]
onlySingleBaseSubs = selections.getToggleStates()[3]
includeIndels = selections.getToggleStates()[4]
parseCustomBed(bedInputFilePaths, genomeFilePath, stratifyByMS,
stratifyByMutSig, separateIndividualCohorts,
onlySingleBaseSubs, includeIndels)
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Bed Mutation Files:", 0,
DataTypeStr.mutations + ".bed",
("Bed Files", ".bed"))
dialog.createDropdown(
"Background Context", 1, 0,
("Singlenuc/Dinuc", "Trinuc/Quadrunuc", "Pentanuc/Hexanuc"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
mutationFilePaths = list(selections.getFilePathGroups())[
0] # A list of paths to the bed mutation files
backgroundContext: str = list(selections.getDropdownSelections())[
0] # What context should be used to generate the background.
# Convert background context to int
if backgroundContext == "Singlenuc/Dinuc":
backgroundContextNum = 1
elif backgroundContext == "Trinuc/Quadrunuc":
backgroundContextNum = 3
elif backgroundContext == "Pentanuc/Hexanuc":
backgroundContextNum = 5
else:
raise ValueError("Matching strings is hard.")
generateMutationBackground(mutationFilePaths, backgroundContextNum)
def getDataDirectory():
# Check for the text file which should contain the path to the data directory.
dataDirectoryTextFilePath = os.path.join(os.getenv("HOME"), ".mutperiod",
"data_dir.txt")
# If it exists, return the directory path within.
if os.path.exists(dataDirectoryTextFilePath):
with open(dataDirectoryTextFilePath, 'r') as dataDirectoryTextFile:
dataDirectory = dataDirectoryTextFile.readline().strip()
# Double check to make sure the data directory is still intact.
# If it isn't, inform the user, and progress through the function to recreate it.
if not os.path.exists(dataDirectory):
print(
"Data directory not found at expected location: {}".format(
dataDirectory))
print(
"Please select a new location to create a data directory.")
else:
return dataDirectory
else:
# Create a simple dialog to select a new data directory location.
from benbiohelpers.TkWrappers.TkinterDialog import TkinterDialog, Selections
checkDirs(os.path.dirname(dataDirectoryTextFilePath))
dialog = TkinterDialog(
workingDirectory=os.path.dirname(dataDirectoryTextFilePath))
dialog.createFileSelector("Location to create new data directory:",
0, ("Fasta Files", ".fa"),
directory=True)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
selections: Selections = dialog.selections
dataDirectoryDirectory = selections.getIndividualFilePaths()[0]
# Make sure a valid, writeable directory was given. Then create the new directory (if it doesn't exist already),
# write it to the text file, and return it! (Also create the __external_data directory.)
if not os.path.exists(dataDirectoryDirectory):
raise UserInputError("Given directory: " + dataDirectoryDirectory +
" does not exist.")
dataDirectory = os.path.join(dataDirectoryDirectory, "mutperiod_data")
try:
checkDirs(dataDirectory)
checkDirs(os.path.join(dataDirectory, "__external_data"))
except IOError:
raise InvalidPathError(
dataDirectoryDirectory,
"Given location for data directory is not writeable:")
with open(dataDirectoryTextFilePath, 'w') as dataDirectoryTextFile:
dataDirectoryTextFile.write(dataDirectory + '\n')
return dataDirectory
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("ICGC Mutation Files:", 0, ".tsv.gz",
("gzip files", ".gz"))
dialog.createFileSelector("Genome Fasta File:", 1, ("Fasta Files", ".fa"))
dialog.createCheckbox("Create individual bed files for each donor.", 2, 0)
dialog.createCheckbox("Stratify results by microsatellite stability", 3, 0)
dialog.createCheckbox("Stratify results by mutation signature", 4, 0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
ICGCFilePaths = list(selections.getFilePathGroups())[
0] # A list of ICGC mutation file paths
genomeFilePath = list(selections.getIndividualFilePaths())[0]
separateDonors = list(selections.getToggleStates())[0]
stratifyByMS = list(selections.getToggleStates())[1]
stratifyByMutSig = list(selections.getToggleStates())[2]
parseICGC(ICGCFilePaths, genomeFilePath, separateDonors, stratifyByMS,
stratifyByMutSig)
def main():
# Create a simple dialog for selecting the gene designation files.
with TkinterDialog(workingDirectory=getDataDirectory()) as dialog:
dialog.createMultipleFileSelector("Bed formatted reads:", 0, ".bed",
("Bed Files", ".bed"))
trimDuplicateReads(dialog.selections.getFilePathGroups()[0])
def main():
with TkinterDialog(workingDirectory=getDataDirectory()) as dialog:
dialog.createMultipleFileSelector("Raw fastq reads:", 0, ".fastq.gz",
("Gzipped fastq Files", ".fastq.gz"))
dialog.createFileSelector("Adaptor Sequences:", 1,
("Fasta Files", ".fa"))
trimAdaptorSequences(dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Feature Files (e.g. mutations):", 0,
"bin_me.bed", ("Bed Files", ".bed"))
dialog.createFileSelector("Gene Designations:", 1, ("Bed Files", ".bed"))
dialog.createCheckbox("Color Domain is present in 7th (index=6) column", 2,
0)
flankDialog = dialog.createDynamicSelector(3, 0)
flankDialog.initCheckboxController(
"Gene designations include flanking regions")
flankSizeDialog = flankDialog.initDisplay(True, "FlankSize")
flankSizeDialog.createTextField("Flanking bin size:",
0,
0,
defaultText="0")
flankSizeDialog.createTextField("Flanking bin number:",
1,
0,
defaultText="0")
flankDialog.initDisplayState()
fileSuffixDialog = dialog.createDynamicSelector(4, 0)
fileSuffixDialog.initCheckboxController("Custom file suffix")
suffixDialog = fileSuffixDialog.initDisplay(True, "Suffix")
suffixDialog.createTextField("File Suffix:", 0, 0, defaultText="all_genes")
fileSuffixDialog.initDisplayState()
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
if dialog.selections.getToggleStates()[0]: colorColIndex = 6
else: colorColIndex = None
if flankDialog.getControllerVar():
flankBinSize = int(dialog.selections.getTextEntries("FlankSize")[0])
flankBinNum = int(dialog.selections.getTextEntries("FlankSize")[1])
else:
flankBinSize = 0
flankBinNum = 0
if fileSuffixDialog.getControllerVar():
fileSuffix = dialog.selections.getTextEntries("Suffix")[0]
else:
fileSuffix = ""
binInGenes(dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0], flankBinSize,
flankBinNum, fileSuffix, colorColIndex)
def main():
# Create a simple dialog for selecting the gene designation files.
dialog = TkinterDialog(workingDirectory=os.path.dirname(__file__))
dialog.createFileSelector("Bed File:", 0, ("bed file", ".bed"))
dialog.mainloop()
if dialog.selections is None: quit()
expandToBothStrands(dialog.selections.getIndividualFilePaths()[0], )
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Mutation Background Files:", 0,
DataTypeStr.mutBackground + ".tsv",
("Tab Seperated Values Files", ".tsv"))
dialog.createMultipleFileSelector("Nucleosome Map Files:", 1,
"nucleosome_map.bed",
("Bed Files", ".bed"))
selectSingleNuc = dialog.createDynamicSelector(2, 0)
selectSingleNuc.initCheckboxController(
"Generate background with a single nucleosome radius (73 bp)")
linkerSelectionDialog = selectSingleNuc.initDisplay(1, "singleNuc")
linkerSelectionDialog.createCheckbox(
"Include 30 bp linker DNA on either side of single nucleosome radius.",
0, 0)
selectSingleNuc.initDisplay(0)
selectSingleNuc.initDisplayState()
dialog.createCheckbox(
"Generate background with a nucleosome group radius (1000 bp)", 3, 0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
mutationBackgroundFilePaths = selections.getFilePathGroups()[
0] # A list of mutation file paths
nucleosomeMapNames = [
getIsolatedParentDir(nucleosomeMapFile)
for nucleosomeMapFile in selections.getFilePathGroups()[1]
]
if selectSingleNuc.getControllerVar():
useSingleNucRadius = True
includeLinker = selections.getToggleStates("singleNuc")[0]
else:
useSingleNucRadius = False
includeLinker = False
useNucGroupRadius = selections.getToggleStates()[0]
if includeLinker: linkerOffset = 30
else: linkerOffset = 0
generateNucleosomeMutationBackground(mutationBackgroundFilePaths,
nucleosomeMapNames,
useSingleNucRadius, useNucGroupRadius,
linkerOffset)
def main():
dialog = TkinterDialog(workingDirectory=os.path.dirname(__file__))
dialog.createFileSelector("Colored Gene Designations:", 0, ("Bed File",".bed"))
dialog.createFileSelector("Colorless Gene Data (e.g. RPKM)", 1, ("Tab separated files",".tsv"))
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
assignToDomainByGene(dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getIndividualFilePaths()[1])
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=os.path.dirname(__file__))
dialog.createFileSelector("Spivakov File:", 0, ("Text File", ".txt"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
parseSpivakovToBed(dialog.selections.getIndividualFilePaths()[0])
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector(
"Background Nucleosome Mutation Counts Files:", 0,
DataTypeStr.nucMutBackground + ".tsv",
("Tab Seperated Values Files", ".tsv"))
customBackgroundSelector = dialog.createDynamicSelector(1, 0)
customBackgroundSelector.initCheckboxController(
"Include files with another data set as custom background.")
customBackgroundFileSelector = customBackgroundSelector.initDisplay(
True, "customBackground")
customBackgroundFileSelector.createMultipleFileSelector(
"Raw nucleosome counts to be normalized", 0,
DataTypeStr.rawNucCounts + ".tsv", ("TSV Files", ".tsv"))
customBackgroundFileSelector.createFileSelector(
"Data Directory for nucleosome counts to be used as background",
1,
directory=True)
customBackgroundSelector.initDisplayState()
dialog.createCheckbox(
"Include alternative scaling factor indepedent of nucleosome map.", 2,
0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
backgroundCountsFilePaths = selections.getFilePathGroups()[
0] # A list of background mutation counts file paths
if customBackgroundSelector.getControllerVar():
customRawCountsFilePaths = selections.getFilePathGroups(
"customBackground")[0]
customBackgroundCountsDir = selections.getIndividualFilePaths(
"customBackground")[0]
else:
customRawCountsFilePaths = None
customBackgroundCountsDir = None
includeAlternativeScaling = selections.getToggleStates()[0]
normalizeCounts(backgroundCountsFilePaths, customRawCountsFilePaths,
customBackgroundCountsDir, includeAlternativeScaling)
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Bed Files:",0,"I_have_bad_chromosomes.bed",("Bed Files",".bed"))
dialog.createFileSelector("Genome Fasta File:",1,("Fasta Files",".fa"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
removeUnacceptableChromosomes(dialog.selections.getFilePathGroups()[0], dialog.selections.getIndividualFilePaths()[0])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createFileSelector("RNAseq File:", 0, ("Bed Files", ".bed"))
dialog.createFileSelector("Gene Designations (color in 7th column):", 1,
("Bed Files", ".bed"))
# Run the UI
dialog.mainloop()
binRNASeqByChromatinDomainInGenes(
dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getIndividualFilePaths()[1], 6)
def main():
with TkinterDialog(workingDirectory = getDataDirectory()) as dialog:
dialog.createMultipleFileSelector("Trimmed fastq reads:", 0, "trimmed.fastq.gz",
("Gzipped fastq Files", ".fastq.gz"))
dialog.createFileSelector("Bowtie2 Index File (Any):", 1, ("Bowtie2 Index File", ".bt2"))
with dialog.createDynamicSelector(2, 0) as bowtie2BinaryDS:
bowtie2BinaryDS.initCheckboxController("Choose alternative bowtie2 binary")
bowtie2BinarySelector = bowtie2BinaryDS.initDisplay(True, selectionsID = "bowtieBinary")
bowtie2BinarySelector.createFileSelector("bowtie2 binary:", 0, ("Any File", "*"))
dialog.createDropdown("Number of CPU threads to use:", 3, 0, ('1', '2', '3', '4'))
with dialog.createDynamicSelector(4, 0) as customArgsDS:
customArgsDS.initDropdownController("Custom Bowtie2 Arguments:", ("None", "From File", "Direct Input"))
customArgsDS.initDisplay("From File", selectionsID = "customArgs").createFileSelector(
"Custom Arguments File:", 0, ("Text File", ".txt")
)
customArgsDS.initDisplay("Direct Input", selectionsID = "customArgs").createTextField(
"Custom Arguments:", 0, 0, defaultText = ''
)
fastqFilePaths = dialog.selections.getFilePathGroups()[0]
bowtie2IndexBasenamePath: str = dialog.selections.getIndividualFilePaths()[0]
bowtie2IndexBasenamePath = bowtie2IndexBasenamePath.rsplit('.', 2)[0]
if bowtie2IndexBasenamePath.endswith(".rev"): bowtie2IndexBasenamePath = bowtie2IndexBasenamePath.rsplit('.', 1)[0]
if bowtie2BinaryDS.getControllerVar():
bowtie2BinaryPath = dialog.selections.getIndividualFilePaths("bowtieBinary")[0]
else: bowtie2BinaryPath = None
threads = dialog.selections.getDropdownSelections()[0]
if customArgsDS.getControllerVar() == "None":
customBowtie2Arguments = ''
elif customArgsDS.getControllerVar() == "From File":
customBowtie2ArgsFilePath = dialog.selections.getIndividualFilePaths("customArgs")[0]
with open(customBowtie2ArgsFilePath, 'r') as customBowtie2ArgsFile:
customBowtie2Arguments = customBowtie2ArgsFile.readline().strip()
elif customArgsDS.getControllerVar() == "Direct Input":
customBowtie2Arguments = dialog.selections.getTextEntries("customArgs")[0]
trimmedFastqToSam(fastqFilePaths, bowtie2IndexBasenamePath,
bowtie2BinaryPath, threads, customBowtie2Arguments)
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Prepared Input Files:",0,DataTypeStr.mutations + ".bed",("bed files",".bed"))
dialog.createFileSelector("Genome Fasta File:",1,("Fasta Files",".fa"))
dialog.createCheckbox("Skip formatting checks for all but the first line",2,0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
parsePreparedInput(dialog.selections.getFilePathGroups()[0], dialog.selections.getIndividualFilePaths()[0],
not dialog.selections.getToggleStates()[0])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createFileSelector("Encompassing Feature File:", 0,
("Bed Files", ".bed"))
dialog.createFileSelector("Nucleosome Dyad Center Positions:", 1,
("Bed Files", ".bed"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
stratifyNucleosomesByEncompassment(
dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getIndividualFilePaths()[1])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Genome Feature Positions Files:", 0,
"context_mutations.bed",
("Bed Files", ".bed"))
dialog.createFileSelector("Gene Ranges File (merged):", 1,
("Bed Files", ".bed"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
splitGenicAndIntergenic(dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0])
def main():
# Create the UI.
with TkinterDialog(workingDirectory=getDataDirectory()) as dialog:
dialog.createFileSelector("SRA run accession IDs:", 0,
("text file", ".txt"))
with dialog.createDynamicSelector(1, 0) as namesDynSel:
namesDynSel.initCheckboxController("Specify Custom Names:")
namesDynSel.initDisplay(True, "namesFilePath").createFileSelector(
"Names File:", 0, ("text file", ".txt"))
# Retrieve values from user input.
selections = dialog.selections
if namesDynSel.getControllerVar():
namesFilePath = selections.getIndividualFilePaths("namesFilePath")[0]
else:
namesFilePath = None
sRA_ToFastq(selections.getIndividualFilePaths()[0], namesFilePath)
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createFileSelector("Bed Mutation Data:", 0, ("Bed Files", ".bed"))
dialog.createFileSelector("TFBS Positions:", 1, ("Bed Files", ".bed"))
dialog.createFileSelector("Output File:",
2, ("TSV Files", ".tsv"),
newFile=True)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
recordMutationsInTFBSs(dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getIndividualFilePaths()[1],
dialog.selections.getIndividualFilePaths()[2])
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Mutation Files:", 0,
DataTypeStr.mutations + ".bed",
("Bed Files", ".bed"))
dialog.createMultipleFileSelector("Binding Motifs Files:", 1,
"binding_motifs.bed",
("Bed Files", ".bed"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
countInBindingMotifs(dialog.selections.getFilePathGroups()[0],
dialog.selections.getFilePathGroups()[1])
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Tab-Separated Nucleosome Counts Files:",0,
DataTypeStr.generalNucCounts + ".tsv",("tsv files",".tsv"))
dialog.createMultipleFileSelector("R Nucleosome Mutation Analysis Files:",1,
".rda",("rda files",".rda"))
fileNumSelector = dialog.createDynamicSelector(2,0)
fileNumSelector.initCheckboxController("Export to one file (as opposed to one file for each graph)")
oneFileDialog = fileNumSelector.initDisplay(1, "oneFile")
oneFileDialog.createFileSelector("Export File", 0, ("pdf file",".pdf"), newFile = True)
manyFilesDialog = fileNumSelector.initDisplay(0, "manyFiles")
manyFilesDialog.createFileSelector("Export Directory", 0, directory = True)
fileNumSelector.initDisplayState()
dialog.createCheckbox("Omit Outliers", 3, 0)
dialog.createCheckbox("Smooth Nuc Group results", 3, 1)
dialog.createCheckbox("Strand align results", 4, 0)
#dialog.createCheckbox("Use normalized values from rda input", 5, 0)
#dialog.createCheckbox("Use raw values from rda input", 5, 1)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections = dialog.selections
tsvFilePaths = selections.getFilePathGroups()[0]
rdaFilePaths = selections.getFilePathGroups()[1]
if fileNumSelector.getControllerVar(): exportPath = selections.getIndividualFilePaths("oneFile")[0]
else: exportPath = selections.getIndividualFilePaths("manyFiles")[0]
omitOutliers = bool(selections.getToggleStates()[0])
smoothNucGroup = bool(selections.getToggleStates()[1])
strandAlign = bool(selections.getToggleStates()[2])
generateFigures(tsvFilePaths, rdaFilePaths, exportPath, omitOutliers, smoothNucGroup,
strandAlign)
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createFileSelector("Data Set Directory:", 0, directory=True)
# TODO: Maybe allow for different cohort selections here. Right now, I'm assuming that we group MS data by mut sigs.
# This will probably work best if it communicates with the project manager to some capacity (what cohort data is available?)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
# Get the user's input from the dialog.
selections: Selections = dialog.selections
dataSetDirectory = selections.getFilePaths()[0]
groupCohorts(dataSetDirectory)
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("CPD Files:", 0, "CPD_data.bed",
("Bed Files", ".bed"))
dialog.createMultipleFileSelector("Deamination Files:", 1,
"deamination_data.bed",
("Bed Files", ".bed"))
dialog.createFileSelector("Genome Fasta File:", 2, ("Fasta File", ".fa"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
parseDeaminationData(dialog.selections.getFilePathGroups()[0],
dialog.selections.getFilePathGroups()[1],
dialog.selections.getIndividualFilePaths()[0])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createFileSelector("Original Nucleosome Map Directory:",
0,
directory=True)
dialog.createMultipleFileSelector("Stratifying Feature Ranges:", 1,
"stratifying_feature_ranges.narrowPeak",
("Bed Files", ".bed"),
("Narrow Peak File", ".narrowPeak"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
stratifyNucleosomeMap(dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getFilePathGroups()[0])
def main():
#Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=os.path.dirname(__file__))
dialog.createMultipleFileSelector("Genome Feature Files:", 0,
DataTypeStr.mutations + ".bed",
("Bed Files", ".bed"))
dialog.createFileSelector("Chromosome Sizes File:", 1,
("Text File", ".txt"))
dialog.createDropdown("Bin Size (bp):", 2, 0,
("1000", "10000", "100000", "1000000"))
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
binAcrossGenome(dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0],
int(dialog.selections.getDropdownSelections()[0]))
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Quartile Files:", 0, "quartile.tsv",
("Tab Separated Files", ".tsv"))
dialog.createFileSelector("Nucleosome Directory:", 1, directory=True)
dialog.createDropdown("Stratification Type", 2, 0, ["h1 density", "other"])
dialog.createCheckbox("Sloppy Copy?", 3, 0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
getQuartileNucleosomePositions(
dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getDropdownSelections()[0].replace(' ', '_'),
dialog.selections.getToggleStates()[0])
def main():
# Create the Tkinter UI
dialog = TkinterDialog(workingDirectory=getDataDirectory())
dialog.createMultipleFileSelector("Genome Feature Positions Files:", 0,
"context_mutations.bed",
("Bed Files", ".bed"))
dialog.createFileSelector("Nucleosome Dyad Center Positions:", 1,
("Bed Files", ".bed"))
dialog.createCheckbox("Only Count Linker", 2, 0)
# Run the UI
dialog.mainloop()
# If no input was received (i.e. the UI was terminated prematurely), then quit!
if dialog.selections is None: quit()
countFeaturesAboutNucleosomes(
dialog.selections.getFilePathGroups()[0],
dialog.selections.getIndividualFilePaths()[0],
dialog.selections.getToggleStates()[0])