def test_add_constraint(self, core_model):
cache = ProblemCache(core_model)
def add_var(model, var_id):
return model.solver.interface.Variable(var_id, ub=0)
def add_constraint(m, const_id, var):
return m.solver.interface.Constraint(var, lb=-10, ub=10, name=const_id)
def update_constraint(model, const, var):
return setattr(const, "ub", 1000)
for i in range(10):
cache.add_variable("%i" % i, add_var, None)
cache.add_constraint("c%i" % i, add_constraint, update_constraint, cache.variables["%i" % i])
for i in range(10):
assert cache.constraints["c%i" % i] in core_model.solver.constraints
assert cache.constraints["c%i" % i].ub == 10
assert cache.constraints["c%i" % i].lb == -10
assert core_model.solver.constraints["c%i" % i].ub == 10
assert core_model.solver.constraints["c%i" % i].lb == -10
for i in range(10):
cache.add_constraint("c%i" % i, add_constraint, update_constraint, cache.variables["%i" % i])
assert core_model.solver.constraints["c%i" % i].ub == 1000
cache.reset()
for i in range(10):
with pytest.raises(KeyError):
core_model.solver.variables.__getitem__("%i" % i)
with pytest.raises(KeyError):
core_model.solver.constraints.__getitem__("c%i" % i)
def __init__(self, model, decoder, objective_function, simulation_method,
simulation_kwargs):
self.model = model
self.decoder = decoder
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
class TargetEvaluator(Evaluator):
"""
Evaluator for targets in a model. It gets a representation, decodes into targets and simulates
the model with a given method.
Attributes
----------
model : SolverBasedModel
A constraint-based model
decoder : SetDecoder
A decoder to convert the representation into knockouts
objective_function : objective_function or list(objective_function)
The objectives of the algorithm
simulation_method : see flux_analysis.simulation
The method use to simulate the knockouts
simulation_kwargs : dict
The extra parameters used by the simulation method
See Also
--------
cameo.strain_design.heuristic.objective_function
Methods
-------
__call__(population)
calling the object will evaluate a population (see inspyred)
"""
def __init__(self, model, decoder, objective_function, simulation_method,
simulation_kwargs):
self.model = model
if not isinstance(decoder, SetDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if not isinstance(objective_function, ObjectiveFunction):
raise ValueError(
"'objective_function' must be instance of ObjectiveFunction (%s)"
% objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __call__(self, population):
return [self._evaluate_individual(tuple(i)) for i in population]
def reset(self):
self.cache.reset()
def _evaluate_individual(self, individual):
raise NotImplementedError
def test_add_variable(self, core_model):
cache = ProblemCache(core_model)
def add_var(model, var_id):
return model.solver.interface.Variable(var_id, ub=0)
def update_var(model, var):
return setattr(var, "ub", 1000)
for i in range(10):
cache.add_variable("%i" % i, add_var, update_var)
for i in range(10):
assert cache.variables["%i" % i] in core_model.solver.variables
assert cache.variables["%i" % i].ub == 0
assert core_model.solver.variables["%i" % i].ub == 0
for i in range(10):
cache.add_variable("%i" % i, add_var, update_var)
assert cache.variables["%i" % i].ub == 1000
assert core_model.solver.variables["%i" % i].ub == 1000
cache.reset()
for i in range(10):
with pytest.raises(KeyError):
core_model.solver.variables.__getitem__("%i" % i)
class TargetEvaluator(Evaluator):
"""
Evaluator for targets in a model. It gets a representation, decodes into targets and simulates
the model with a given method.
Attributes
----------
model : cobra.Model
A constraint-based model
decoder : SetDecoder
A decoder to convert the representation into knockouts
objective_function : objective_function or list(objective_function)
The objectives of the algorithm
simulation_method : see flux_analysis.simulation
The method use to simulate the knockouts
simulation_kwargs : dict
The extra parameters used by the simulation method
See Also
--------
cameo.strain_design.heuristic.objective_function
Methods
-------
__call__(population)
calling the object will evaluate a population (see inspyred)
"""
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
if not isinstance(decoder, SetDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if not isinstance(objective_function, ObjectiveFunction):
raise ValueError("'objective_function' must be instance of ObjectiveFunction (%s)" % objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __call__(self, population):
return [self.evaluate_individual(tuple(i)) for i in population]
def reset(self):
self.cache.reset()
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
self.decoder = decoder
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __init__(self, model, decoder, objective_function, simulation_method,
simulation_kwargs):
self.model = model
if not isinstance(decoder, SetDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if not isinstance(objective_function, ObjectiveFunction):
raise ValueError(
"'objective_function' must be instance of ObjectiveFunction (%s)"
% objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def test_lmoma_with_cache(self, core_model):
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
lmoma(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("u_") for v in core_model.solver.variables)
assert any(c.name.startswith("lmoma_const_") for c in core_model.solver.constraints)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("u_") for v in core_model.solver.variables)
assert not any(c.name.startswith("lmoma_const_") for c in core_model.solver.constraints)
def test_lmoma_with_cache(self, core_model):
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
lmoma(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("u_") for v in core_model.solver.variables)
assert any(c.name.startswith("lmoma_const_") for c in core_model.solver.constraints)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("u_") for v in core_model.solver.variables)
assert not any(c.name.startswith("lmoma_const_") for c in core_model.solver.constraints)
def test_cache_problem(self, problem_cache_trial):
core_model, reference, n_constraints, n_variables = problem_cache_trial
# After the number of variables and constraints remains the same if nothing happens
assert n_constraints == len(core_model.solver.constraints)
assert n_variables == len(core_model.solver.variables)
cache = ProblemCache(core_model)
some_method_that_adds_stuff(core_model, cache)
# After running some_method_that_adds_stuff with cache, problem has 10 more variables
assert n_variables + 10 == len(core_model.solver.variables)
# And has 9 more more constraints
assert n_constraints + 9 == len(core_model.solver.constraints)
cache.reset()
# After reset cache, the problem should return to its original size
assert n_constraints == len(core_model.solver.constraints)
assert n_variables == len(core_model.solver.variables)
def test_moma_with_cache(self, core_model):
if current_solver_name(core_model) == 'glpk':
pytest.skip('glpk does not support qp')
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
moma(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("moma_aux_") for v in core_model.solver.variables)
assert any(c.name.startswith("moma_const_") for c in core_model.solver.constraints)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("moma_aux_") for v in core_model.solver.variables)
assert not any(c.name.startswith("moma_const_") for c in core_model.solver.constraints)
def test_moma_with_cache(self, core_model):
if current_solver_name(core_model) == 'glpk':
pytest.skip('glpk does not support qp')
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
moma(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("moma_aux_") for v in core_model.solver.variables)
assert any(c.name.startswith("moma_const_") for c in core_model.solver.constraints)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("moma_aux_") for v in core_model.solver.variables)
assert not any(c.name.startswith("moma_const_") for c in core_model.solver.constraints)
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
if not isinstance(decoder, SetDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if not isinstance(objective_function, ObjectiveFunction):
raise ValueError("'objective_function' must be instance of ObjectiveFunction (%s)" % objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def test_room_with_cache(self, core_model):
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
infeasible = 0
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
try:
room(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("y_") for v in core_model.solver.variables)
assert any(c.name.startswith("room_const_") for c in core_model.solver.constraints)
except OptimizationError: # TODO: room shouldn't return infeasible for non-essential reactions
infeasible += 1
continue
assert infeasible < len(core_model.reactions)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("y_") for v in core_model.solver.variables)
assert not any(c.name.startswith("room_const_") for c in core_model.solver.constraints)
def test_room_with_cache(self, core_model):
original_objective = core_model.objective
pfba_solution = pfba(core_model)
essential_reactions = find_essential_reactions(core_model)
cache = ProblemCache(core_model)
infeasible = 0
for r in core_model.reactions:
if r not in essential_reactions:
with core_model:
r.knock_out()
try:
room(core_model, reference=pfba_solution, cache=cache)
assert any(v.name.startswith("y_") for v in core_model.solver.variables)
assert any(c.name.startswith("room_const_") for c in core_model.solver.constraints)
except OptimizationError: # TODO: room shouldn't return infeasible for non-essential reactions
infeasible += 1
continue
assert infeasible < len(core_model.reactions)
cache.reset()
assert core_model.objective.expression == original_objective.expression
assert not any(v.name.startswith("y_") for v in core_model.solver.variables)
assert not any(c.name.startswith("room_const_") for c in core_model.solver.constraints)
def test_with(self, problem_cache_trial):
core_model, reference, n_constraints, n_variables = problem_cache_trial
with ProblemCache(core_model) as cache:
some_method_that_adds_stuff(core_model, cache)
# After running some_method_that_adds_stuff with cache, problem has 10 more variables
assert n_variables + 10 == len(core_model.solver.variables)
# And has 9 more more constraints
assert n_constraints + 9 == len(core_model.solver.constraints)
# If the method runs again, it does not add repeated variables
some_method_that_adds_stuff(core_model, cache)
# After running some_method_that_adds_stuff with cache, problem has 10 more variables
assert n_variables + 10 == len(core_model.solver.variables)
# And has 9 more more constraints
assert n_constraints + 9 == len(core_model.solver.constraints)
# After reset cache, the problem should return to its original size
assert n_constraints == len(core_model.solver.constraints)
assert n_variables == len(core_model.solver.variables)
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
if not isinstance(decoder, KnockoutDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if isinstance(objective_function, list):
if not len(objective_function) > 0:
raise ValueError("list of objectives cannot be empty")
invalid = []
for index, of in enumerate(objective_function):
if not isinstance(of, ObjectiveFunction):
invalid.append((index, of))
if len(invalid) > 0:
raise ValueError("objectives %s must be instance of ObjectiveFunction (%s)"
% (",".join(str(i[0]) for i in invalid), [i[1] for i in invalid]))
elif not isinstance(objective_function, ObjectiveFunction):
raise ValueError("'objective_function' must be instance of ObjectiveFunction (%s)" % objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
class KnockoutEvaluator(object):
"""
Evaluator for knockouts. It gets a representation, decodes into knockouts and simulates
the model with a given method.
Attributes
----------
model : SolverBasedModel
A constraint-based model
decoder : KnockoutDecoder
A decoder to convert the representation into knockouts
objective_function : objective_function or list(objective_function)
The objectives of the algorithm
simulation_method : see flux_analysis.simulation
The method use to simulate the knockouts
simulation_kwargs : dict
The extra parameters used by the simulation method
See Also
--------
cameo.strain_design.heuristic.objective_function
Methods
-------
__call__(population)
calling the object will evaluate a population (see inspyred)
"""
def __init__(self, model, decoder, objective_function, simulation_method,
simulation_kwargs):
self.model = model
self.decoder = decoder
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __call__(self, population):
res = [self.evaluate_individual(frozenset(i)) for i in population]
self.cache.reset()
return res
@memoize
def evaluate_individual(self, individual):
decoded = self.decoder(individual)
reactions = decoded[0]
with TimeMachine() as tm:
for reaction in reactions:
reaction.knock_out(time_machine=tm)
try:
solution = self.simulation_method(self.model,
cache=self.cache,
volatile=False,
raw=True,
**self.simulation_kwargs)
fitness = self._calculate_fitness(solution, decoded)
except SolveError as e:
logger.debug(e)
if isinstance(self.objective_function, list):
fitness = inspyred.ec.emo.Pareto(
values=[0 for _ in self.objective_function])
else:
fitness = 0
return fitness
def _calculate_fitness(self, solution, decoded):
if isinstance(self.objective_function, list):
logger.debug("evaluate multi objective")
return inspyred.ec.emo.Pareto(values=[
of(self.model, solution, decoded)
for of in self.objective_function
])
else:
logger.debug("evaluate single objective")
return self.objective_function(self.model, solution, decoded)
def moma(model, reference=None, cache=None, reactions=None, *args, **kwargs):
"""
Minimization of Metabolic Adjustment[1]
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
cache: ProblemCache
reactions: list
Returns
-------
FluxDistributionResult
Contains the result of the solver.
References
----------
.. [1] Segrè, D., Vitkup, D., & Church, G. M. (2002). Analysis of optimality in natural and perturbed metabolic
networks. Proceedings of the National Academy of Sciences of the United States of America, 99(23), 15112–7.
doi:10.1073/pnas.232349399
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
cache.begin_transaction()
try:
for rid, flux_value in six.iteritems(reference):
def create_variable(model, variable_id):
var = model.solver.interface.Variable(variable_id)
return var
var_id = "moma_aux_%s" % rid
cache.add_variable(var_id, create_variable, None)
def create_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression - var,
lb=flux_value,
ub=flux_value,
name=constraint_id)
return constraint
def update_constraint(model, constraint, var, reaction, flux_value):
if constraint.lb != flux_value:
constraint.lb = flux_value
constraint.ub = flux_value
constraint_id = "moma_const_%s" % rid
reaction = model.reactions.get_by_id(rid)
cache.add_constraint(constraint_id, create_constraint, update_constraint,
cache.variables[var_id], reaction, flux_value)
def create_objective(model, variables):
return model.solver.interface.Objective(Add(*[FloatOne * var ** 2 for var in variables]),
direction="min",
sloppy=True)
cache.add_objective(create_objective, None, cache.variables.values())
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult({r: solution.get_primal_by_id(r) for r in reactions}, solution.f)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
class KnockoutEvaluator(object):
"""
Evaluator for knockouts. It gets a representation, decodes into knockouts and simulates
the model with a given method.
Attributes
----------
model : SolverBasedModel
A constraint-based model
decoder : KnockoutDecoder
A decoder to convert the representation into knockouts
objective_function : objective_function or list(objective_function)
The objectives of the algorithm
simulation_method : see flux_analysis.simulation
The method use to simulate the knockouts
simulation_kwargs : dict
The extra parameters used by the simulation method
See Also
--------
cameo.strain_design.heuristic.objective_function
Methods
-------
__call__(population)
calling the object will evaluate a population (see inspyred)
"""
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
if not isinstance(decoder, KnockoutDecoder):
raise ValueError("Invalid decoder %s" % decoder)
self.decoder = decoder
if isinstance(objective_function, list):
if not len(objective_function) > 0:
raise ValueError("list of objectives cannot be empty")
invalid = []
for index, of in enumerate(objective_function):
if not isinstance(of, ObjectiveFunction):
invalid.append((index, of))
if len(invalid) > 0:
raise ValueError("objectives %s must be instance of ObjectiveFunction (%s)"
% (",".join(str(i[0]) for i in invalid), [i[1] for i in invalid]))
elif not isinstance(objective_function, ObjectiveFunction):
raise ValueError("'objective_function' must be instance of ObjectiveFunction (%s)" % objective_function)
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __call__(self, population):
return [self._evaluate_individual(tuple(i)) for i in population]
def reset(self):
self.cache.reset()
@property
def is_mo(self):
return isinstance(self.objective_function, list)
@memoize
def _evaluate_individual(self, individual):
"""
Evaluates a single individual.
Arguments
---------
individual: set
The encoded representation of a single individual.
Returns
-------
fitness
A single real value or a Pareto, depending on the number of objectives.
"""
decoded = self.decoder(individual)
reactions = decoded[0]
with TimeMachine() as tm:
for reaction in reactions:
reaction.knock_out(time_machine=tm)
try:
solution = self.simulation_method(self.model,
cache=self.cache,
volatile=False,
raw=True,
reactions=reactions2filter(self.objective_function),
**self.simulation_kwargs)
fitness = self._calculate_fitness(solution, decoded)
except SolveError as e:
logger.debug(e)
if self.is_mo:
fitness = inspyred.ec.emo.Pareto(values=[0 for _ in self.objective_function])
else:
fitness = 0.
return fitness
def _calculate_fitness(self, solution, decoded):
if self.is_mo:
logger.debug("evaluate multiobjective solution")
return inspyred.ec.emo.Pareto(values=[of(self.model, solution, decoded) for of in self.objective_function])
else:
logger.debug("evaluate single objective solution")
return self.objective_function(self.model, solution, decoded)
def lmoma(model, reference=None, cache=None, *args, **kwargs):
"""Linear Minimization Of Metabolic Adjustment.
Parameters
----------
model: SolverBasedModel
reference: dict
objective: str or reaction or optlang.Objective
An objective to be minimized/maximized for
volatile: boolean
cache: dict
Returns
-------
FluxDistributionResult
Contains the result of the linear solver.
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
cache.begin_transaction()
if not isinstance(reference, (dict, FluxDistributionResult)):
raise TypeError("reference must be a flux distribution (dict or FluxDistributionResult")
try:
for rid, flux_value in six.iteritems(reference):
reaction = model.reactions.get_by_id(rid)
def create_variable(model, var_id, lb):
var = model.solver.interface.Variable(var_id, lb=lb)
return var
pos_var_id = "u_%s_pos" % rid
cache.add_variable(pos_var_id, create_variable, None, 0)
neg_var_id = "u_%s_neg" % rid
cache.add_variable(neg_var_id, create_variable, None, 0)
# ui = vi - wt
def update_upper_constraint(model, constraint, var, reaction, flux_value):
constraint.lb = flux_value
def create_upper_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression + var,
lb=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("c_%s_ub" % rid, create_upper_constraint, update_upper_constraint,
cache.variables[pos_var_id], reaction, flux_value)
def update_lower_constraint(model, constraint, var, reaction, flux_value):
constraint.ub = flux_value
def create_lower_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression - var,
ub=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("c_%s_lb" % rid, create_lower_constraint, update_lower_constraint,
cache.variables[neg_var_id], reaction, flux_value)
model.objective = model.solver.interface.Objective(add([mul([One, var]) for var in cache.variables.values()]),
direction="min")
try:
return FluxDistributionResult(model.solve())
except SolveError as e:
raise e
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
class KnockoutEvaluator(object):
"""
Evaluator for knockouts. It gets a representation, decodes into knockouts and simulates
the model with a given method.
Attributes
----------
model : SolverBasedModel
A constraint-based model
decoder : KnockoutDecoder
A decoder to convert the representation into knockouts
objective_function : objective_function or list(objective_function)
The objectives of the algorithm
simulation_method : see flux_analysis.simulation
The method use to simulate the knockouts
simulation_kwargs : dict
The extra parameters used by the simulation method
See Also
--------
cameo.strain_design.heuristic.objective_function
Methods
-------
__call__(population)
calling the object will evaluate a population (see inspyred)
"""
def __init__(self, model, decoder, objective_function, simulation_method, simulation_kwargs):
self.model = model
self.decoder = decoder
self.objective_function = objective_function
self.simulation_method = simulation_method
self.simulation_kwargs = simulation_kwargs
self.cache = ProblemCache(model)
def __call__(self, population):
res = [self.evaluate_individual(frozenset(i)) for i in population]
self.cache.reset()
return res
@memoize
def evaluate_individual(self, individual):
decoded = self.decoder(individual)
reactions = decoded[0]
with TimeMachine() as tm:
for reaction in reactions:
reaction.knock_out(time_machine=tm)
try:
solution = self.simulation_method(self.model,
cache=self.cache,
volatile=False,
raw=True,
**self.simulation_kwargs)
fitness = self._calculate_fitness(solution, decoded)
except SolveError as e:
logger.debug(e)
if isinstance(self.objective_function, list):
fitness = inspyred.ec.emo.Pareto(values=[0 for _ in self.objective_function])
else:
fitness = 0
return fitness
def _calculate_fitness(self, solution, decoded):
if isinstance(self.objective_function, list):
logger.debug("evaluate multi objective")
return inspyred.ec.emo.Pareto(values=[of(self.model, solution, decoded) for of in self.objective_function])
else:
logger.debug("evaluate single objective")
return self.objective_function(self.model, solution, decoded)
def room(model, reference=None, cache=None, delta=0.03, epsilon=0.001, reactions=None, *args, **kwargs):
"""Regulatory On/Off Minimization [1].
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
delta: float
epsilon: float
cache: ProblemCache
Returns
-------
FluxDistributionResult
Contains the result of the linear solver.
References
----------
.. [1] Tomer Shlomi, Omer Berkman and Eytan Ruppin, "Regulatory on/off minimization of metabolic
flux changes after genetic perturbations", PNAS 2005 102 (21) 7695-7700; doi:10.1073/pnas.0406346102
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
elif not isinstance(cache, ProblemCache):
raise TypeError("Invalid cache object (must be a cameo.util.ProblemCache)")
cache.begin_transaction()
if not isinstance(reference, (dict, pandas.Series, FluxDistributionResult)):
raise TypeError("reference must be a flux distribution (dict or FluxDistributionResult")
try:
for rid, flux_value in six.iteritems(reference):
reaction = model.reactions.get_by_id(rid)
def create_variable(model, var_id):
return model.solver.interface.Variable(var_id, type="binary")
cache.add_variable("y_%s" % rid, create_variable, None)
def create_upper_constraint(model, constraint_id, reaction, variable, flux_value, epsilon):
w_u = flux_value + delta * abs(flux_value) + epsilon
return model.solver.interface.Constraint(
reaction.flux_expression - variable * (reaction.upper_bound - w_u),
ub=w_u,
sloppy=True,
name=constraint_id)
def update_upper_constraint(model, constraint, reaction, variable, flux_value, epsilon):
w_u = flux_value + delta * abs(flux_value) + epsilon
constraint.set_linear_coefficients({variable: reaction.upper_bound - w_u})
constraint.ub = w_u
cache.add_constraint("room_const_%s_upper" % rid, create_upper_constraint, update_upper_constraint,
reaction, cache.variables["y_%s" % rid], flux_value, epsilon)
def create_lower_constraint(model, constraint_id, reaction, variable, flux_value, epsilon):
w_l = flux_value - delta * abs(flux_value) - epsilon
return model.solver.interface.Constraint(
reaction.flux_expression - variable * (reaction.lower_bound - w_l),
lb=w_l,
sloppy=True,
name=constraint_id)
def update_lower_constraint(model, constraint, reaction, variable, flux_value, epsilon):
w_l = flux_value - delta * abs(flux_value) - epsilon
constraint.set_linear_coefficients({variable: reaction.lower_bound - w_l})
constraint.lb = w_l
cache.add_constraint("room_const_%s_lower" % rid, create_lower_constraint, update_lower_constraint,
reaction, cache.variables["y_%s" % rid], flux_value, epsilon)
model.objective = model.solver.interface.Objective(add([mul([One, var]) for var in cache.variables.values()]),
direction='min')
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult(
{r: solution.get_primal_by_id(r) for r in reactions}, solution.objective_value)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
def lmoma(model, reference=None, cache=None, reactions=None, *args, **kwargs):
"""Linear Minimization Of Metabolic Adjustment [1].
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
cache: ProblemCache
reactions: list
Returns
-------
FluxDistributionResult
Contains the result of the solver.
References
----------
.. [1] Becker, S. A., Feist, A. M., Mo, M. L., Hannum, G., Palsson, B. Ø., & Herrgard, M. J. (2007).
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox.
Nature Protocols, 2(3), 727–38. doi:10.1038/nprot.2007.99
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
cache.begin_transaction()
if not isinstance(reference, (dict, pandas.Series, FluxDistributionResult)):
raise TypeError("reference must be a flux distribution (dict or FluxDistributionResult")
try:
for rid, flux_value in six.iteritems(reference):
reaction = model.reactions.get_by_id(rid)
def create_variable(model, var_id, lb):
var = model.solver.interface.Variable(var_id, lb=lb)
return var
pos_var_id = "u_%s_pos" % rid
cache.add_variable(pos_var_id, create_variable, None, 0)
neg_var_id = "u_%s_neg" % rid
cache.add_variable(neg_var_id, create_variable, None, 0)
# ui = vi - wt
def update_upper_constraint(model, constraint, var, reaction, flux_value):
if constraint.lb != flux_value:
constraint.lb = flux_value
def create_upper_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression + var,
lb=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("lmoma_const_%s_ub" % rid, create_upper_constraint, update_upper_constraint,
cache.variables[pos_var_id], reaction, flux_value)
def update_lower_constraint(model, constraint, var, reaction, flux_value):
if constraint.ub != flux_value:
constraint.ub = flux_value
def create_lower_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression - var,
ub=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("lmoma_const_%s_lb" % rid, create_lower_constraint, update_lower_constraint,
cache.variables[neg_var_id], reaction, flux_value)
def create_objective(model, variables):
return model.solver.interface.Objective(add([mul((One, var)) for var in variables]),
direction="min",
sloppy=False)
cache.add_objective(create_objective, None, cache.variables.values())
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult(
{r: solution.get_primal_by_id(r) for r in reactions}, solution.objective_value)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
def moma(model, reference=None, cache=None, reactions=None, *args, **kwargs):
"""
Minimization of Metabolic Adjustment[1]
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
cache: ProblemCache
reactions: list
Returns
-------
FluxDistributionResult
Contains the result of the solver.
References
----------
.. [1] Segrè, D., Vitkup, D., & Church, G. M. (2002). Analysis of optimality in natural and perturbed metabolic
networks. Proceedings of the National Academy of Sciences of the United States of America, 99(23), 15112–7.
doi:10.1073/pnas.232349399
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
cache.begin_transaction()
try:
for rid, flux_value in six.iteritems(reference):
def create_variable(model, variable_id):
var = model.solver.interface.Variable(variable_id)
return var
var_id = "moma_aux_%s" % rid
cache.add_variable(var_id, create_variable, None)
def create_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression - var,
lb=flux_value,
ub=flux_value,
name=constraint_id)
return constraint
def update_constraint(model, constraint, var, reaction, flux_value):
if constraint.lb != flux_value:
constraint.lb = flux_value
constraint.ub = flux_value
constraint_id = "moma_const_%s" % rid
reaction = model.reactions.get_by_id(rid)
cache.add_constraint(constraint_id, create_constraint, update_constraint,
cache.variables[var_id], reaction, flux_value)
def create_objective(model, variables):
return model.solver.interface.Objective(Add(*[FloatOne * var ** 2 for var in variables]),
direction="min",
sloppy=True)
cache.add_objective(create_objective, None, cache.variables.values())
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult(
{r: solution.get_primal_by_id(r) for r in reactions}, solution.objective_value)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
def room(model, reference=None, cache=None, delta=0.03, epsilon=0.001, reactions=None, *args, **kwargs):
"""Regulatory On/Off Minimization [1].
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
delta: float
epsilon: float
cache: ProblemCache
Returns
-------
FluxDistributionResult
Contains the result of the linear solver.
References
----------
.. [1] Tomer Shlomi, Omer Berkman and Eytan Ruppin, "Regulatory on/off minimization of metabolic
flux changes after genetic perturbations", PNAS 2005 102 (21) 7695-7700; doi:10.1073/pnas.0406346102
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
elif not isinstance(cache, ProblemCache):
raise TypeError("Invalid cache object (must be a cameo.util.ProblemCache)")
cache.begin_transaction()
if not isinstance(reference, (dict, pandas.Series, FluxDistributionResult)):
raise TypeError("reference must be a flux distribution (dict or FluxDistributionResult")
try:
for rid, flux_value in six.iteritems(reference):
reaction = model.reactions.get_by_id(rid)
def create_variable(model, var_id):
return model.solver.interface.Variable(var_id, type="binary")
cache.add_variable("y_%s" % rid, create_variable, None)
def create_upper_constraint(model, constraint_id, reaction, variable, flux_value, epsilon):
w_u = flux_value + delta * abs(flux_value) + epsilon
return model.solver.interface.Constraint(
reaction.flux_expression - variable * (reaction.upper_bound - w_u),
ub=w_u,
sloppy=True,
name=constraint_id)
def update_upper_constraint(model, constraint, reaction, variable, flux_value, epsilon):
w_u = flux_value + delta * abs(flux_value) + epsilon
constraint.set_linear_coefficients({variable: reaction.upper_bound - w_u})
constraint.ub = w_u
cache.add_constraint("room_const_%s_upper" % rid, create_upper_constraint, update_upper_constraint,
reaction, cache.variables["y_%s" % rid], flux_value, epsilon)
def create_lower_constraint(model, constraint_id, reaction, variable, flux_value, epsilon):
w_l = flux_value - delta * abs(flux_value) - epsilon
return model.solver.interface.Constraint(
reaction.flux_expression - variable * (reaction.lower_bound - w_l),
lb=w_l,
sloppy=True,
name=constraint_id)
def update_lower_constraint(model, constraint, reaction, variable, flux_value, epsilon):
w_l = flux_value - delta * abs(flux_value) - epsilon
constraint.set_linear_coefficients({variable: reaction.lower_bound - w_l})
constraint.lb = w_l
cache.add_constraint("room_const_%s_lower" % rid, create_lower_constraint, update_lower_constraint,
reaction, cache.variables["y_%s" % rid], flux_value, epsilon)
model.objective = model.solver.interface.Objective(add([mul([One, var]) for var in cache.variables.values()]),
direction='min')
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult({r: solution.get_primal_by_id(r) for r in reactions}, solution.f)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()
# print("cobra pfba")
# tic = time.time()
# optimize_minimal_flux(cb_model, solver='cglpk')
# print("flux sum:", sum([abs(val) for val in list(cb_model.solution.x_dict.values())]))
# print("cobra pfba runtime:", time.time() - tic)
print("pfba")
tic = time.time()
ref = pfba(model)
print("flux sum:", end=' ')
print(sum([abs(val) for val in list(ref.values())]))
print("cameo pfba runtime:", time.time() - tic)
print("lmoma")
cache = ProblemCache(model)
tic = time.time()
res = lmoma(model, reference=ref, cache=cache)
print("flux distance:", end=' ')
print(sum([abs(res[v] - ref[v]) for v in list(res.keys())]))
print("cameo lmoma runtime:", time.time() - tic)
print("room")
tic = time.time()
res = room(model, reference=ref)
print(sum([abs(res[v] - ref[v]) for v in list(res.keys())]))
print("cameo room runtime:", time.time() - tic)
print("flux distance:", end=' ')
print(sum([abs(res[v] - ref[v]) for v in list(res.keys())]))
print("sum yi:", res.objective_value)
def lmoma(model, reference=None, cache=None, reactions=None, *args, **kwargs):
"""Linear Minimization Of Metabolic Adjustment [1].
Parameters
----------
model: cobra.Model
reference: FluxDistributionResult, dict
cache: ProblemCache
reactions: list
Returns
-------
FluxDistributionResult
Contains the result of the solver.
References
----------
.. [1] Becker, S. A., Feist, A. M., Mo, M. L., Hannum, G., Palsson, B. Ø., & Herrgard, M. J. (2007).
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox.
Nature Protocols, 2(3), 727–38. doi:10.1038/nprot.2007.99
"""
volatile = False
if cache is None:
volatile = True
cache = ProblemCache(model)
cache.begin_transaction()
if not isinstance(reference, (dict, pandas.Series, FluxDistributionResult)):
raise TypeError("reference must be a flux distribution (dict or FluxDistributionResult")
try:
for rid, flux_value in six.iteritems(reference):
reaction = model.reactions.get_by_id(rid)
def create_variable(model, var_id, lb):
var = model.solver.interface.Variable(var_id, lb=lb)
return var
pos_var_id = "u_%s_pos" % rid
cache.add_variable(pos_var_id, create_variable, None, 0)
neg_var_id = "u_%s_neg" % rid
cache.add_variable(neg_var_id, create_variable, None, 0)
# ui = vi - wt
def update_upper_constraint(model, constraint, var, reaction, flux_value):
if constraint.lb != flux_value:
constraint.lb = flux_value
def create_upper_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression + var,
lb=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("lmoma_const_%s_ub" % rid, create_upper_constraint, update_upper_constraint,
cache.variables[pos_var_id], reaction, flux_value)
def update_lower_constraint(model, constraint, var, reaction, flux_value):
if constraint.ub != flux_value:
constraint.ub = flux_value
def create_lower_constraint(model, constraint_id, var, reaction, flux_value):
constraint = model.solver.interface.Constraint(reaction.flux_expression - var,
ub=flux_value,
sloppy=True,
name=constraint_id)
return constraint
cache.add_constraint("lmoma_const_%s_lb" % rid, create_lower_constraint, update_lower_constraint,
cache.variables[neg_var_id], reaction, flux_value)
def create_objective(model, variables):
return model.solver.interface.Objective(add([mul((One, var)) for var in variables]),
direction="min",
sloppy=False)
cache.add_objective(create_objective, None, cache.variables.values())
solution = model.optimize(raise_error=True)
if reactions is not None:
result = FluxDistributionResult({r: solution.get_primal_by_id(r) for r in reactions}, solution.f)
else:
result = FluxDistributionResult.from_solution(solution)
return result
except Exception as e:
cache.rollback()
raise e
finally:
if volatile:
cache.reset()