def run(self, workspace): image = workspace.image_set.get_image(self.image_name.value, must_be_grayscale=True) pixel_data = image.pixel_data if self.wants_automatic_object_size.value: object_size = min(30, max(1, np.mean(pixel_data.shape) / 40)) else: object_size = float(self.object_size.value) sigma = object_size / 2.35 if self.smoothing_method.value == GAUSSIAN_FILTER: def fn(image): return scind.gaussian_filter(image, sigma, mode="constant", cval=0) output_pixels = smooth_with_function_and_mask(pixel_data, fn, image.mask) elif self.smoothing_method.value == MEDIAN_FILTER: output_pixels = median_filter(pixel_data, image.mask, object_size / 2 + 1) elif self.smoothing_method.value == SMOOTH_KEEPING_EDGES: sigma_range = float(self.sigma_range.value) output_pixels = bilateral_filter(pixel_data, image.mask, sigma, sigma_range) elif self.smoothing_method.value == FIT_POLYNOMIAL: output_pixels = fit_polynomial(pixel_data, image.mask, self.clip.value) elif self.smoothing_method.value == CIRCULAR_AVERAGE_FILTER: output_pixels = circular_average_filter(pixel_data, object_size / 2 + 1, image.mask) elif self.smoothing_method.value == SM_TO_AVERAGE: if image.has_mask: mean = np.mean(pixel_data[image.mask]) else: mean = np.mean(pixel_data) output_pixels = np.ones(pixel_data.shape, pixel_data.dtype) * mean else: raise ValueError("Unsupported smoothing method: %s" % self.smoothing_method.value) output_image = cpi.Image(output_pixels, parent_image=image) workspace.image_set.add(self.filtered_image_name.value, output_image) workspace.display_data.pixel_data = pixel_data workspace.display_data.output_pixels = output_pixels
def run(self, workspace): image = workspace.image_set.get_image(self.image_name.value, must_be_grayscale=True) pixel_data = image.pixel_data if self.wants_automatic_object_size.value: object_size = min(30, max(1, np.mean(pixel_data.shape) / 40)) else: object_size = float(self.object_size.value) sigma = object_size / 2.35 if self.smoothing_method.value == GAUSSIAN_FILTER: def fn(image): return scind.gaussian_filter(image, sigma, mode="constant", cval=0) output_pixels = smooth_with_function_and_mask( pixel_data, fn, image.mask) elif self.smoothing_method.value == MEDIAN_FILTER: output_pixels = median_filter(pixel_data, image.mask, object_size / 2 + 1) elif self.smoothing_method.value == SMOOTH_KEEPING_EDGES: sigma_range = np.float(self.sigma_range.value) output_pixels = skimage.restoration.denoise_bilateral( image=pixel_data.astype(np.float), multichannel=image.multichannel, sigma_color=sigma_range, sigma_spatial=sigma, ) elif self.smoothing_method.value == FIT_POLYNOMIAL: output_pixels = fit_polynomial(pixel_data, image.mask, self.clip.value) elif self.smoothing_method.value == CIRCULAR_AVERAGE_FILTER: output_pixels = circular_average_filter(pixel_data, object_size / 2 + 1, image.mask) elif self.smoothing_method.value == SM_TO_AVERAGE: if image.has_mask: mean = np.mean(pixel_data[image.mask]) else: mean = np.mean(pixel_data) output_pixels = np.ones(pixel_data.shape, pixel_data.dtype) * mean else: raise ValueError("Unsupported smoothing method: %s" % self.smoothing_method.value) output_image = cellprofiler_core.image.Image(output_pixels, parent_image=image) workspace.image_set.add(self.filtered_image_name.value, output_image) workspace.display_data.pixel_data = pixel_data workspace.display_data.output_pixels = output_pixels
def run_per_layer(self, image, channel): if channel >= 0: pixel_data = image.pixel_data[:,:,channel].squeeze() else: pixel_data = image.pixel_data mask = image.mask if self.wants_automatic_object_size.value: object_size = min(30, max(1, np.mean(pixel_data.shape) / 40)) else: object_size = float(self.object_size.value) sigma = object_size / 2.35 if self.smoothing_method.value == GAUSSIAN_FILTER: def fn(image): return scind.gaussian_filter(image, sigma, mode='constant', cval=0) output_pixels = smooth_with_function_and_mask(pixel_data, fn, mask) elif self.smoothing_method.value == MEDIAN_FILTER: output_pixels = median_filter(pixel_data, mask, object_size / 2 + 1) elif self.smoothing_method.value == SMOOTH_KEEPING_EDGES: sigma_range = float(self.sigma_range.value) output_pixels = bilateral_filter(pixel_data, mask, sigma, sigma_range) elif self.smoothing_method.value == FIT_POLYNOMIAL: output_pixels = fit_polynomial(pixel_data, mask, self.clip.value) elif self.smoothing_method.value == CIRCULAR_AVERAGE_FILTER: output_pixels = circular_average_filter(pixel_data, object_size / 2 + 1, mask) elif self.smoothing_method.value == SM_TO_AVERAGE: if image.has_mask: mean = np.mean(pixel_data[mask]) else: mean = np.mean(pixel_data) output_pixels = np.ones(pixel_data.shape, pixel_data.dtype) * mean elif self.smoothing_method.value == REMOVE_OUTLIER: # TODO: implement how this deals with masks. nbhood = self.outlierneighbourhood.value output_pixels = self.remove_outlier_pixels(pixel_data, threshold=self.treshold.value, radius=nbhood, mode='max') else: raise ValueError("Unsupported smoothing method: %s" % self.smoothing_method.value) return output_pixels
def run_grayscale(self, pixel_data, image): if self.wants_automatic_object_size.value: object_size = min(30, max(1, np.mean(pixel_data.shape) / 40)) else: object_size = float(self.object_size.value) sigma = object_size / 2.35 if self.smoothing_method.value == GAUSSIAN_FILTER: def fn(image): return scind.gaussian_filter(image, sigma, mode='constant', cval=0) output_pixels = smooth_with_function_and_mask( pixel_data, fn, image.mask) elif self.smoothing_method.value == MEDIAN_FILTER: output_pixels = median_filter(pixel_data, image.mask, object_size / 2 + 1) elif self.smoothing_method.value == SMOOTH_KEEPING_EDGES: sigma_range = float(self.sigma_range.value) output_pixels = skimage.restoration.denoise_bilateral( image=pixel_data, multichannel=image.multichannel, sigma_color=sigma_range, sigma_spatial=sigma) elif self.smoothing_method.value == FIT_POLYNOMIAL: output_pixels = fit_polynomial(pixel_data, image.mask, self.clip.value) elif self.smoothing_method.value == CIRCULAR_AVERAGE_FILTER: output_pixels = circular_average_filter(pixel_data, object_size / 2 + 1, image.mask) elif self.smoothing_method.value == SM_TO_AVERAGE: if image.has_mask: mean = np.mean(pixel_data[image.mask]) else: mean = np.mean(pixel_data) output_pixels = np.ones(pixel_data.shape, pixel_data.dtype) * mean else: raise ValueError("Unsupported smoothing method: %s" % self.smoothing_method.value) return output_pixels