def do(sequences, query_seq, position, pattern, changed_pattern, SW=0):
'''Finds the difference in the entropy in patterns
when a pattern is changed to changed pattern.
'''
# case 1: for original amino acid
sequences.append(query_seq)
patterns = check_mutation_position.do(sequences, position)
if SW:
sw = landgraf_sequence_weights.do(sequences)
else:
sw = list(np.ones(len(sequences)))
entropy1 = calculate_sop(patterns, sw)
# case 2: for mutant amino acid
sequences.pop()
new_query_seq = util.strsub(query_seq, position, changed_pattern)
sequences.append(new_query_seq)
patterns = check_mutation_position.do(sequences, position)
if SW:
sw = landgraf_sequence_weights.do(sequences)
else:
sw = list(np.ones(len(sequences)))
entropy2 = calculate_sop(patterns, sw)
return (entropy2 - entropy1)
def main():
filename = '../alignments/Q92481'
position = 72
changed_pattern = 'R'
import load_alignments
import uniquify_alignments
import check_mutation_position
import correct_alignment_position
proteins = load_alignments.do(filename)
sorted_list = sorted(proteins, key=itemgetter('match_percentage'))
proteins = sorted_list
proteins.reverse()
proteins = uniquify_alignments.do(proteins)
query, prots = util.fetch_query_protein_in_alignments(proteins)
mod_position = correct_alignment_position.do(query['alignment'], position)
pattern = query['alignment'][mod_position]
patterns = check_mutation_position.do(
[prot['alignment'] for prot in proteins], mod_position)
print(do(patterns, pattern, changed_pattern))
def do(sequences):
L = len(sequences[0]) # length of aligned sequences
N = len(sequences) # number of sequences
weights = []
for i in range(L):
aa = check_mutation_position.do(sequences, i)
freq = util.calc_frequency(aa)
uniq_aa = util.unique(aa)
# compute sequence weights
w = []
for i in range(N):
if aa[i] == '-' or aa[i] == 'X':
w.append(0)
else:
if aa[i] == 'B':
aa[i] = 'N'
elif aa[i] == 'Z':
aa[i] = 'Q'
w.append(1.0 / (len(uniq_aa) * freq[aa[i]]))
weights.append(w) # N x L matrix, for each position find the weights
# compute average of w over all positions
avg_weight = np.zeros(N)
for i in range(L):
avg_weight += np.array(weights[i])
avg_weight *= 1.0 / L
return avg_weight
if len(alignments) == 0:
continue
# fetch actual position of mutation in aligned query sequence
try:
actual_pos = correct_alignment_position.do(query['alignment'],
position)
except Exception as e:
print(query['alignment'])
continue
# fetch the corresponding sequences
sequences = [a['alignment'] for a in alignments]
# compute frequency of amino acid at desired position
aa = check_mutation_position.do([k['alignment'] for k in alignments],
actual_pos)
# compute mean value in the column
mean = {}
cnt = 0
for key in values.keys(): # for each physco property
first = 1
for z in aa:
if first:
if z != '-':
mean[key] = values[key][z]
cnt += 1
first = 0
else:
continue
else:
def work(line):
'''This method computes all kinds of scores,
for a given mutation, and returns the computed scores.
Args:
line: which contains mutation info.
<PROTEIN_ID, POSITION, ORIGINAL_AMINO_ACID, MUTANT_AMINO_ACID>
Return Values:
[parts, scores] flattend list
'''
line = line.strip()
parts = line.split(',')
protein = parts[0]
position = int(parts[1]) - 1 # correction for indexing python lists
mut_aa = parts[3]
result = []
print(protein)
for alignment_dir in align_dirs:
alignment_file = os.path.join(alignment_dir, protein)
if not os.path.isfile(alignment_file):
return []
fasta = util.read_sequence('../fasta/%s.fasta' % protein)
proteins = load_alignments.do(
alignment_file) # types of proteins required
# sort & prune the list of proteins
proteins = prune_proteins_list(proteins)
# fetch the record pertaining to the query protein
query, p = util.fetch_query_protein_in_alignments(
proteins) # p does not contain query sequence
prots = [prot["id"] for prot in proteins]
types = [prot["type"] for prot in proteins]
match_percents = [prot["match_percentage"] for prot in proteins]
alignments = [prot["alignment"] for prot in proteins]
proteins = prots #replacing proteins dicitonary with only ids
result.append(len(prots) + 1)
orig_aa = fasta[position]
# correct the position of query protein wrt to alignment
mod_pos = correct_alignment_position.do(query['alignment'], position)
aa = check_mutation_position.do(alignments, mod_pos)
# calculate shannon entropy score w/o sequence weights
result.append(
shannon_entropy_score.do(list(alignments), query['alignment'],
mod_pos, orig_aa, mut_aa))
# shannon entropy score with sequence weights
result.append(
shannon_entropy_score.do(list(alignments), query['alignment'],
mod_pos, orig_aa, mut_aa, 1))
# calculate von-neumann entropy score
result.append(von_neumann_entropy_score.do(list(aa), orig_aa, mut_aa))
# calculate relative entropy score
#result.append(relative_entropy_score.do(list(aa), orig_aa, mut_aa))
# calculate jensen-shannon divergence score
#result.append(jensen_shannon_divergence_score(list(aa), orig_aa, mut_aa))
# calculate sum-of-pairs scores
result.append(
sum_of_pairs_score.do(list(alignments), query['alignment'],
mod_pos, orig_aa, mut_aa, 0)) # wo seq wg
result.append(
sum_of_pairs_score.do(list(alignments), query['alignment'],
mod_pos, orig_aa, mut_aa, 1)) # w seq wg
# return mutation information and scores for recording in a file
return [str(item) for item in list(chain.from_iterable([parts, result]))]
def do(file1, file2):
# fetch all mutations
mutations = []
with open(file1) as fp:
for line in fp:
parts = line.strip().split(',')
mutations.append(
[parts[0], parts[1], parts[2], parts[3], parts[4]])
ofp = open(file2, 'w')
for mut in mutations:
# fetch reqd info
protein = mut[0]
position = int(mut[1]) - 1
orig_aa = mut[2]
mut_aa = mut[3]
# if protein not aligned - pass this
alignment_file = '../alignments/%s' % protein
if not os.path.isfile(alignment_file):
continue
print(mut)
# load the aligned sequences
alignments = load_alignments.do(alignment_file)
# prune the proteins
alignments = util.prune_proteins_list(alignments)
# fetch query sequence
query, alignments = util.fetch_query_protein_in_alignments(alignments)
if len(alignments) == 0:
continue
# fetch actual position of mutation in aligned query sequence
try:
actual_pos = correct_alignment_position.do(query['alignment'],
position)
except Exception as e:
print(query['alignment'])
continue
# fetch the corresponding sequences
sequences = [a['alignment'] for a in alignments]
# compute frequency of amino acid at desired position
aa = check_mutation_position.do([k['alignment'] for k in alignments],
actual_pos)
# compute simple frequency of original & mutant amino acid
o_score, m_score = simple_frequency_score.do(list(aa), orig_aa, mut_aa)
# compute score using pseudo-counts in order to account for missing aa
o_ps_score, m_ps_score = pseudo_count_score.do(list(aa), orig_aa,
mut_aa)
# compute simple sequence-weighted frequency score
sequence_weights = landgraf_sequence_weights.do(
[a['alignment'] for a in alignments])
o_sw_score, m_sw_score = simple_frequency_score.do(
list(aa), orig_aa, mut_aa, sequence_weights)
# using gap frequencies
o_gf_score, m_gf_score = gapped_frequency_score.do(
list(aa), orig_aa, mut_aa, sequence_weights)
# calculate shannon entropy score w/o sequence weights
shannon = shannon_entropy_score.do(list(sequences), query['alignment'],
actual_pos, orig_aa, mut_aa)
# shannon entropy score with sequence weights
shannon_weighted = shannon_entropy_score.do(list(sequences),
query['alignment'],
actual_pos, orig_aa,
mut_aa, 1)
# calculate von-neumann entropy score
von_neumann_score = von_neumann_entropy_score.do(
list(aa), orig_aa, mut_aa)
# calculate sum-of-pairs scores
sop = sum_of_pairs_score.do(list(sequences), query['alignment'],
actual_pos, orig_aa, mut_aa,
0) # wo seq wg
sop_wg = sum_of_pairs_score.do(list(sequences), query['alignment'],
actual_pos, orig_aa, mut_aa,
1) # w seq wg
# append all scores together and write to file
scores = [
o_score, m_score, o_ps_score, m_ps_score, o_sw_score, m_sw_score,
o_gf_score, m_gf_score, shannon, shannon_weighted,
von_neumann_score, sop, sop_wg
]
#print(scores)
ofp.write(','.join(
[str(item)
for item in list(chain.from_iterable([mut, scores]))]) + '\n')
#break
ofp.close()
def do(file1, file2, winsize):
mutations = []
with open(file1) as fp:
for line in fp:
mutations.append(line.strip().split(','))
with open(file2, 'w') as ofp:
for mut in mutations:
print mut
flag = 0
protein = mut[0]
position = int(mut[1]) - 1
orig_aa = mut[2]
mut_aa = mut[3]
fasta_seq = util.read_sequence('../fasta/%s.fasta' % protein)
alfile = '../alignments/%s' % protein
if not os.path.isfile(alfile):
continue
proteins = load_alignments.do(alfile)
proteins = util.prune_proteins_list(proteins)
query, proteins = util.fetch_query_protein_in_alignments(proteins)
query_seq = query['alignment']
alignments = [a['alignment'] for a in proteins]
try:
sequence_weights = landgraf_sequence_weights.do(alignments)
except Exception as e:
print str(e)
flag = 1
if flag:
continue
scores = []
for w in range(winsize + 1): # 0,1,2,3 for winsize=3, hence the +1
try:
# what score to use?
if w == 0:
mod_pos = correct_alignment_position.do(
query_seq, position)
aa = check_mutation_position.do(alignments, mod_pos)
o, m = gapped_frequency_score.do(
list(aa), orig_aa, mut_aa,
sequence_weights) # mod_pos
scores.append(m)
else:
mod_pos = correct_alignment_position.do(
query_seq, position - w)
aa = check_mutation_position.do(alignments, mod_pos)
aa_in_fasta = fasta_seq[position - w]
o, m = gapped_frequency_score.do(
list(aa), aa_in_fasta, mut_aa,
sequence_weights) # mod_pos
scores.append(
o
) # left neighbour at position w from mutation position
mod_pos = correct_alignment_position.do(
query_seq, position + w)
aa = check_mutation_position.do(alignments, mod_pos)
aa_in_fasta = fasta_seq[position + w]
o, m = gapped_frequency_score.do(
list(aa), aa_in_fasta, mut_aa,
sequence_weights) # mod_pos
scores.append(
o
) # right neighbour at position w from mutation position
except Exception:
flag = 1
break
if flag:
continue
#print(scores)
ofp.write(','.join([
str(item) for item in list(chain.from_iterable([mut, scores]))
]) + '\n')
def do(file1, file2):
# fetch all mutations
mutations = []
with open(file1) as fp:
for line in fp:
parts = line.strip().split(',')
mutations.append(
[parts[0], parts[1], parts[2], parts[3], parts[4]])
# ofp = open(file2,'w')
for mut in mutations:
# fetch reqd info
protein = mut[0]
position = int(mut[1]) - 1
orig_aa = mut[2]
mut_aa = mut[3]
# if protein not aligned - pass this
alignment_file = '../alignments/%s' % protein
if not os.path.isfile(alignment_file):
continue
print(mut)
# load the aligned sequences
alignments = load_alignments.do(alignment_file)
# remove duplicate sequences in aligned sequences
alignments = uniquify_alignments.do(alignments)
# fetch query sequence
query, alignments = util.fetch_query_protein_in_alignments(alignments)
if len(alignments) == 0:
continue
# fetch actual position of mutation in aligned query sequence
try:
actual_pos = correct_alignment_position.do(query['alignment'],
position)
except Exception as e:
print(query['alignment'])
continue
# compute frequency of amino acid at desired position
aa = check_mutation_position.do([k['alignment'] for k in alignments],
actual_pos)
# compute simple frequency of original & mutant amino acid
o_score, m_score = simple_frequency_score.do(aa, orig_aa, mut_aa)
# compute score using pseudo-counts in order to account for missing aa
o_ps_score, m_ps_score = pseudo_count_score.do(aa, orig_aa, mut_aa)
# compute simple sequence-weighted frequency score
sequence_weights = landgraf_sequence_weights.do(
[a['alignment'] for a in alignments])
o_sw_score, m_sw_score = simple_frequency_score.do(
aa, orig_aa, mut_aa, sequence_weights)
# using gap frequencies
o_gf_score, m_gf_score = gapped_frequency_score.do(
aa, orig_aa, mut_aa, sequence_weights)
# append all scores together and write to file
scores = [
o_score, m_score, o_ps_score, m_ps_score, o_sw_score, m_sw_score,
o_gf_score, m_gf_score
]
print(scores)
# ofp.write(','.join([ str(item) for item in list(chain.from_iterable([mut, scores])) ])+'\n')
break
