def test_ncoi_cut(self): '''Test standard TypeII cutter.''' ncoi = RestrictionSite(DNA('CCATGG'), (1, 5), name='NcoI') assert_equal(reaction.digest(self.dna, ncoi), [DNA('TGAC----', bottom='CATGGTCA'), DNA('CATGGAAA', bottom='TTTC----')]) assert_equal(reaction.digest(self.dna.circularize(), ncoi), [DNA('CATGGAAATGAC----', bottom='CATGGTCATTTC----')])
def test_ncoi_cut(self): '''Test standard TypeII cutter.''' ncoi = RestrictionSite(DNA('CCATGG'), (1, 5), name='NcoI') assert_equal(reaction.digest(self.dna, ncoi), [ DNA('TGAC----', bottom='CATGGTCA'), DNA('CATGGAAA', bottom='TTTC----') ]) assert_equal(reaction.digest(self.dna.circularize(), ncoi), [DNA('CATGGAAATGAC----', bottom='CATGGTCATTTC----')])
def test_psti_cut(self): '''Test 3\' cutter.''' psti = RestrictionSite(DNA('CTGCAG'), (5, 1), name='PstI') assert_equal( reaction.digest(DNA('ACTGCAGA'), psti), [DNA('ACTGCA', bottom='----GT'), DNA('----GA', bottom='TCTGCA')])
def test_psti_cut(self): '''Test 3\' cutter.''' psti = RestrictionSite(DNA('CTGCAG'), (5, 1), name='PstI') assert_equal(reaction.digest(DNA('ACTGCAGA'), psti), [DNA('ACTGCA', bottom='----GT'), DNA('----GA', bottom='TCTGCA')])
def test_ecorv_cut(self): '''Test blunt-end cutter.''' ecorv = RestrictionSite(DNA('GATATC'), (3, 3), name='EcoRV') assert_equal(reaction.digest(DNA('GATATC'), ecorv), [DNA('GAT'), DNA('ATC')])
def test_not_found(self): '''If site not found, should return input sequence in list.''' ecorv = RestrictionSite(DNA('GATATC'), (3, 3), name='EcoRV') assert_equal(self.dna, reaction.digest(self.dna, ecorv)[0])