class EMEulerExplorer(EM3DSymModel,Animator):
def mousePressEvent(self,event):
if self.events_mode == "inspect":
self.current_hit = self.get_hit(event)
if self.current_hit == None:
EM3DSymModel.mousePressEvent(self,event)
else:
EM3DSymModel.mousePressEvent(self,event)
def mouseReleaseEvent(self,event):
if self.events_mode == "inspect":
if self.current_hit != None:
self.updateGL() # there needs to be a clear or something in order for the picking to work. This is bit of hack but our rendering function doesn't take long anyhow
hit = self.get_hit(event)
if hit == self.current_hit:
self.emit(QtCore.SIGNAL("point_selected"),self.current_hit,event)
else:
#EM3DSymModel.mouseReleaseEvent(self,event)
EM3DModel.mouseReleaseEvent(self, event) #behavior in EM3DSymModel is not what we want (needed in sibling classes?)
self.current_hit = None
else:
#EM3DSymModel.mouseReleaseEvent(self,event)
EM3DModel.mouseReleaseEvent(self, event) #behavior in EM3DSymModel is not what we want (needed in sibling classes?)
def mouseMoveEvent(self,event):
if self.events_mode == "inspect" and self.current_hit:
pass
else:
EM3DSymModel.mouseMoveEvent(self,event)
def get_hit(self,event):
v = self.vdtools.wview.tolist()
self.get_gl_widget().makeCurrent() # prevents a stack underflow
# x = event.x()
# y = v[-1]-event.y()
# glClear(GL_COLOR_BUFFER_BIT | GL_DEPTH_BUFFER_BIT | GL_STENCIL_BUFFER_BIT )
# vals = self.render(color_picking=True)
# glFlush()
# vv = glReadPixels(x,y,1,1,GL_RGB,GL_FLOAT)
# reslt = Vec3f(float(vv[0][0][0]),float(vv[0][0][1]),float(vv[0][0][2]))
# for i,val in enumerate(vals):
## print val,reslt,(reslt-val).length(),vv[0][0]
# if (reslt-val).length() < 0.01:
# print i
## print (reslt-val).length()
# return i
# print vv
#
# the problem with this approach is that depth testing is not part of picking
sb = [0 for i in xrange(0,512)]
glSelectBuffer(512)
glRenderMode(GL_SELECT)
glInitNames()
glMatrixMode(GL_PROJECTION)
glPushMatrix()
glLoadIdentity()
gluPickMatrix(event.x(),v[-1]-event.y(),5,5,v)
self.get_gl_widget().load_perspective()
glMatrixMode(GL_MODELVIEW)
glInitNames()
self.render()
glMatrixMode(GL_PROJECTION)
glPopMatrix()
glMatrixMode(GL_MODELVIEW)
glFlush()
intersection = None
hits = list(glRenderMode(GL_RENDER))
for hit in hits:
a,b,c=hit
if len(c) > 0:
intersection = c[0]-1
break
return intersection
def keyPressEvent(self,event):
if event.key() == Qt.Key_F1:
self.display_web_help("http://blake.bcm.edu/emanwiki/EMAN2/Programs/e2eulerxplor")
elif event.key() == Qt.Key_F :
if self.flatten>0 : self.flatten=0.0
else: self.flatten=1.0
self.generate_current_display_list(True)
self.updateGL()
else:
EM3DSymModel.keyPressEvent(self,event)
def __init__(self, gl_widget=None, auto=True,sparse_mode=False, file_name = ""):
self.current_hit = None
self.events_mode_list = ["navigate", "inspect"]
self.events_mode = self.events_mode_list[1]
self.init_lock = True # a lock indicated that we are still in the __init__ function
self.au_data = None # This will be a dictionary, keys will be refinement directories, values will be something like available iterations for visual study
if auto: # this a flag that tells the eulerxplorer to search for refinement data and automatically add elements to the inspector, if so
self.gen_refinement_data()
EM3DSymModel.__init__(self, gl_widget, eulerfilename=file_name)
Animator.__init__(self)
self.height_scale = 8.0 # This is a value used in EM3DSymModel which scales the height of the displayed cylinders - I made it 8 because it seemed fine. The user can change it anyhow
self.projection_file = None # This is a string - the name of the projection images file
self.average_file = None # This is a string - the name of the class averages file
self.proj_class_viewer = None # This will be an EMImageMXWidget that shows the class and/or projection
self.particle_viewer = None # This will be an EMImageMXWidget that shows the particles in a class
self.clsdb = None # I think this will become redundant - it used to be the old database that stores which particles are in a class, but now that's stored in the header
self.particle_file = None # This will be a string - the name of the file that has the particle files in it. This might be made redundant with the new approach
self.alignment_file = None # This will be a string - the name of the file containing the alignment parameters - this is essential if you we want to show the aligned particles
self.refine_dir = None # This will be a string - the name of the current refinement directory that is being studied
self.dx = None # This is an EMData object storing the x shifts of the alignments for all particles. Generated by e2classaverage
self.dy = None # This is an EMData object storing the y shifts of the alignments for all particles. Generated by e2classaverage
self.da = None# This is an EMData object storing the angle of the alignments for all particles. Generated by e2classaverage
self.dflip = None # This is an EMData object storing whether or not tthe alignment involved a flip, for all particles. Generated by e2classaverage
self.classes = None # This is an EMData object storing which class(es) a particle belongs to. Generated by e2classaverage
self.inclusions = None # This is and EMDAta storing a boolean that indicates the particle was actually included in the final average. Generated by e2classaverage
self.average = None # This the class average itself, an EMData object
self.projection = None # This is the projection itelse, an EMData object
self.class_idx = None # This is the idx of the current class being studied in the interface
self.previous_len = -1 # To keep track of the number of class averages that were previously viewable. This helps to make sure we can switch to the same class average in the context of a different refinement iteration
self.mirror_eulers = False
if sparse_mode:
self.mirror_eulers = True # If True the drawn Eulers are are also rendered on the opposite side of the sphere - see EM3DSymModel.make_sym_dl_lis
# Grab the symmetry from the workflow database if possible
sym = "c1"
if js_check_dict("refine_01/0_refine_parms.json"):
try: sym = str(js_open_dict("refine_01/0_refine_parms.json")["sym"])
except: pass
# Have to tell the EM3DSymModel that there is a new sym
self.set_symmetry(sym)
# this object will have
if self.au_data != None:
combo_entries = self.au_data.keys()
combo_entries.sort()
combo_entries.reverse()
if len(combo_entries) > 0:
au = combo_entries[0]
cls = self.au_data[au][0][0]
self.au_selected(au,cls)
self.mirror_eulers = True
self.init_lock = False
self.force_update=True # Force a display udpdate in EMImage3DSymModule
QtCore.QObject.connect(self, QtCore.SIGNAL("point_selected"), self.au_point_selected)
def __del__(self):
EM3DSymModel.__del__(self) # this is here for documentation purposes - beware that the del function is important
def initializeGL(self):
glEnable(GL_NORMALIZE)
def generate_current_display_list(self,force=False):
'''
Redefinition of EMImage3DSymModule.generate_current_display_list
'''
if self.init_lock: return 0
if self.au_data == None or len(self.au_data) == 0:
EM3DSymModel.generate_current_display_list(self,force)
self.init_basic_shapes()
if self.nomirror == True : val = 0
else: val = 1
self.trace_great_arcs(self.sym_object.get_asym_unit_points(val))
self.trace_great_triangles(val)
self.eulers = self.specified_eulers
if self.eulers == None: return 0
# if not self.colors_specified: self.point_colors = []
# else: self.point_colors = self.specified_colors
# self.points = []
# for i in self.eulers:
# p = i.transpose()*Vec3f(0,0,self.radius)
# self.points.append(p)
# if not self.colors_specified: self.point_colors.append((0.34615, 0.3143, 0.0903,1))
self.make_sym_dl_list(self.eulers)
return 1
def get_data_dims(self):
return (2*self.radius,2*self.radius,2*self.radius)
def width(self): return 2*self.radius
def height(self): return 2*self.radius
def depth(self): return 2*self.radius
def gen_refinement_data(self):
dirs,files = get_files_and_directories()
dirs.sort()
for i in range(len(dirs)-1,-1,-1):
if len(dirs[i]) != 9:
dirs.pop(i)
elif dirs[i][:7] != "refine_":
dirs.pop(i)
else:
try: int(dirs[i][7:])
except: dirs.pop(i)
self.dirs = dirs
print dirs
self.au_data = {}
for dir in self.dirs:
d = self.check_refine_db_dir(dir)
if len(d) != 0 and len(d[dir]) != 0: self.au_data.update(d)
def check_refine_db_dir(self,dir,s1="classes",s2=None,s3="cls_result",s4="threed",s5="projections"):
# s2 used to be class_indices
names = [s1,s2,s3,s4,s5]
data = {}
data[dir] = []
register_js_name = "{}/0_refine_parms.json".format(dir)
files=os.listdir(dir)
try:
nums=[int(i[7:9]) for i in files if "threed" in i and "even" not in i and "odd" not in i]
maxnum=max(nums)
except :
print "Nothing in ",dir
return {}
for i in xrange(1,maxnum+1):
exte="_{:02d}_even.hdf".format(i)
exto="_{:02d}_odd.hdf".format(i)
data[dir].append([sadd(dir,s1,exte),sadd(dir,s2,exte),sadd(dir,s3,exte),sadd(dir,s4,exte),sadd(dir,s5,exte)])
data[dir].append([sadd(dir,s1,exto),sadd(dir,s2,exto),sadd(dir,s3,exto),sadd(dir,s4,exto),sadd(dir,s5,exto)])
return data
def set_projection_file(self,projection_file): self.projection_file = projection_file
def get_inspector(self):
if not self.inspector :
if (self.au_data == None or len(self.au_data) == 0) and self.mirror_eulers == False: #self.mirror_eulers thing is a little bit of a hack, it's tied to the sparse_mode flag in the init function, which is used by euler_display in EMAN2.py
self.inspector=EMAsymmetricUnitInspector(self,True,True)
else:
self.inspector=EMAsymmetricUnitInspector(self)
QtCore.QObject.connect(self.inspector,QtCore.SIGNAL("au_selected"),self.au_selected)
return self.inspector
def au_selected(self,refine_dir,cls):
self.refine_dir = refine_dir
get_application().setOverrideCursor(Qt.BusyCursor)
data = []
for d in self.au_data[refine_dir]:
if d[0] == cls:
data = d;
break
if len(data) == 0:
error("error, no data for %s %s, returning" %(refine_dir,cls))
# print "error, no data for",au,cls,"returning"
self.events_handlers["inspect"].reset()
get_application().setOverrideCursor(Qt.ArrowCursor)
return
try :
self.particle_file=js_open_dict(refine_dir+"/0_refine_parms.json")["input"]
except:
error("No data in "+refine_dir )
self.events_handlers["inspect"].reset()
get_application().setOverrideCursor(Qt.ArrowCursor)
return
self.average_file = cls
self.projection_file = data[4]
self.alignment_file = data[2]
self.clsdb = data[1]
self.dx = None
self.dy = None
self.da = None
self.dflip = None
self.classes = None
eulers = get_eulers_from(self.average_file)
#s = Symmetries.get("d7")
#eulers = s.gen_orientations("rand",{"n":EMUtil.get_image_count(self.average_file)})
self.specify_eulers(eulers)
#from emimagemx import EMDataListCache
#a = EMData.read_images(self.average_file)
#a = [test_image() for i in range(EMUtil.get_image_count(self.average_file))]
#print len(a),len(eulers)
#b = [a[i].set_attr("xform.projection",eulers[i]) for i in range(len(eulers))]
#b = [a[i].set_attr("ptcl_repr",1) for i in range(len(eulers))]
self.set_emdata_list_as_data(EMLightWeightParticleCache.from_file(self.average_file),"ptcl_repr")
#self.set_emdata_list_as_data(EMDataListCache(self.average_file),"ptcl_repr")
# self.set_emdata_list_as_data(a,"ptcl_repr")
self.force_update = True
self.au_point_selected(self.class_idx,None)
# if we have the same number of Eulers we can update everything
# if self.previous_len == len(eulers) : self.events_handlers["inspect"].repeat_event()
# else:self.events_handlers["inspect"].reset()
self.previous_len = len(eulers)
if not self.init_lock:self.updateGL()
get_application().setOverrideCursor(Qt.ArrowCursor)
def __get_file_headers(self,filename):
headers = []
n = EMUtil.get_image_count(filename)
for i in range(n):
e = EMData()
e.read_image(filename,i,True)
headers.append(e)
return headers
def au_point_selected(self,i,event=None):
if i == None:
if event != None and event.modifiers()&Qt.ShiftModifier:
if self.special_euler != None:
self.special_euler = None
if not self.init_lock:self.regen_dl()
return
# self.arc_anim_points = None
self.projection = None
if self.euler_data:
# db = db_open_dict(self.average_file)
# a = db.get(i)
# print a["nx"]
# print self.average_file,i
# self.average = EMData(self.average_file,i)
# self.average["nx"]
self.average = self.euler_data[i]#
self.projection = EMData(self.projection_file,self.average.get_attr("projection_image_idx"))
self.average.process_inplace("normalize.toimage",{"to":self.projection})
try:
self.class_idx = self.average.get_attr("projection_image_idx")
print "%d (%d)"%(self.class_idx,self.average["ptcl_repr"])
except:
self.class_idx = -1
else: return
#if self.projection == None and self.average == None: return
first = False
if self.proj_class_viewer == None:
first = True
self.proj_class_viewer = EMImageMXWidget(data=None,application=get_application())
# self.proj_class_viewer = EMImage2DWidget(image=None,application=get_application())
QtCore.QObject.connect(self.proj_class_viewer,QtCore.SIGNAL("module_closed"),self.on_mx_view_closed)
# self.proj_class_viewer.set_mouse_mode("App" )
QtCore.QObject.connect(self.proj_class_viewer,QtCore.SIGNAL("mx_image_selected"), self.mx_image_selected)
get_application().show_specific(self.proj_class_viewer)
self.proj_class_single = EMImage2DWidget(image=None,application=get_application())
QtCore.QObject.connect(self.proj_class_single,QtCore.SIGNAL("module_closed"),self.on_mx_view_closed)
# QtCore.QObject.connect(self.proj_class_single,QtCore.SIGNAL("mx_image_selected"), self.mx_image_selected)
get_application().show_specific(self.proj_class_single)
disp = []
if self.projection != None: disp.append(self.projection)
if self.average != None and self.projection!=None:
# ok, this really should be put into its own processor
#dataf = self.projection.do_fft()
#apix=self.projection["apix_x"]
#curve = dataf.calc_radial_dist(dataf["ny"], 0, 0.5,True)
#curve=[i/(dataf["nx"]*dataf["ny"])**2 for i in curve]
#xcurve=[i/(apix*2.0*dataf["ny"]) for i in range(len(curve))]
#xyd=XYData()
#xyd.set_xy_list(xcurve,curve)
#filt=self.average.process("filter.setstrucfac",{"apix":apix,"strucfac":xyd})
#filt.process_inplace("normalize.toimage",{"to":self.average})
self.projection["apix_x"]=self.average["apix_x"]
self.projection["apix_y"]=self.average["apix_y"]
self.projection["apix_z"]=self.average["apix_z"]
filt=self.projection.process("threshold.notzero")
filt.mult(self.average)
filt.process_inplace("filter.matchto",{"to":self.projection})
disp.append(filt)
if self.average!=None:
disp.append(self.average)
self.proj_class_viewer.set_data(disp)
self.proj_class_single.set_data(disp)
self.proj_class_viewer.updateGL()
self.proj_class_single.updateGL()
if self.particle_viewer != None:
self.mx_image_selected(None,None)
if first: self.proj_class_viewer.optimally_resize()
if i != self.special_euler:
self.special_euler = i
self.force_update = True
if not self.init_lock: self.updateGL()
def on_mx_view_closed(self):
self.proj_class_viewer = None
self.proj_class_single = None
def on_particle_mx_view_closed(self):
self.particle_viewer = None
def animation_done_event(self,animation):
pass
def alignment_time_animation(self,transforms):
if len(transforms) < 2: return
animation = OrientationListAnimation(self,transforms,self.radius)
self.register_animatable(animation)
def particle_selected(self,event,lc):
if lc != None:
d = lc[3]
ptcl_idx = d["Img #"]
data = self.au_data[self.refine_dir]
prj = []
cls_result = []
for l in data:
for s in l:
stag = base_name(s)
if len(stag) > 11 and stag[:11] == "projections":
prj.append(s)
elif len(stag) > 10 and stag[:10] == "cls_result":
cls_result.append(s)
transforms = []
if len(prj) != len(cls_result): RunTimeError("The number of cls_result files does not match the number of projection files?")
e = EMData()
for i,cr in enumerate(cls_result):
r = Region(0,ptcl_idx,1,1)
e.read_image(cr,0,False,r)
p = int(e.get(0))
e.read_image(prj[i],p,True)
transforms.append(e["xform.projection"])
self.alignment_time_animation(transforms)
def mx_image_selected(self,event,lc):
# self.arc_anim_points = None
get_application().setOverrideCursor(Qt.BusyCursor)
if lc != None: self.sel = lc[0]
if self.average != None:
included = []
if self.average.has_attr("class_ptcl_idxs"):
included = self.average["class_ptcl_idxs"]
excluded = []
if self.average.has_attr("exc_class_ptcl_idxs"):
excluded = self.average["exc_class_ptcl_idxs"]
all = included + excluded
#all.sort()
bdata = []
data = []
idx_included = []
running_idx = 0
from emimagemx import ApplyAttribute
for val in included:
bdata.append([self.particle_file,val,[ApplyAttribute("Img #",val)]])
idx_included.append(running_idx)
running_idx += 1
idx_excluded = []
for val in excluded:
bdata.append([self.particle_file,val,[ApplyAttribute("Img #",val)]])
idx_excluded.append(running_idx)
running_idx += 1
data = EMLightWeightParticleCache(bdata)
first = False
if self.particle_viewer == None:
first = True
self.particle_viewer = EMImageMXWidget(data=None,application=get_application())
self.particle_viewer.set_mouse_mode("App" )
QtCore.QObject.connect(self.particle_viewer,QtCore.SIGNAL("module_closed"),self.on_particle_mx_view_closed)
QtCore.QObject.connect(self.particle_viewer,QtCore.SIGNAL("mx_image_selected"), self.particle_selected)
get_application().show_specific(self.particle_viewer)
self.check_images_in_memory()
if self.sel== 0 or self.alignment_file == None:
self.particle_viewer.set_data(data)
else:
for i,[name,idx,f] in enumerate(bdata):
index = -1
if self.classes.get_xsize() == 1:
index = 0 # just assume it's the first one - this is potentially fatal assumption, but in obscure situations only
else:
for j in range(self.classes.get_xsize()):
if int(self.classes.get(j,idx)) == self.class_idx:
index = j
break
if index == -1:
print "couldn't find"
get_application().setOverrideCursor(Qt.ArrowCursor)
return
x = self.dx.get(index,idx)
y = self.dy.get(index,idx)
a = self.da.get(index,idx)
m = self.dflip.get(index,idx)
t = Transform({"type":"2d","alpha":a,"mirror":int(m)})
t.set_trans(x,y)
from emimagemx import ApplyTransform
f.append(ApplyTransform(t))
#data[i].transform(t)
self.particle_viewer.set_data(data)
if first:
self.particle_viewer.updateGL()
self.particle_viewer.optimally_resize()
self.particle_viewer.clear_sets(False)
self.particle_viewer.enable_set("Excluded",idx_excluded,True,False)
self.particle_viewer.enable_set("Included",idx_included,False,False)
self.particle_viewer.updateGL()
get_application().setOverrideCursor(Qt.ArrowCursor)
self.updateGL()
def check_images_in_memory(self):
if self.alignment_file != None:
if self.dx == None:
self.dx = EMData(self.alignment_file,2)
if self.dy == None:
self.dy = EMData(self.alignment_file,3)
if self.da == None:
self.da = EMData(self.alignment_file,4)
if self.dflip == None:
self.dflip = EMData(self.alignment_file,5)
if self.classes == None:
self.classes = EMData(self.alignment_file,0)
if self.inclusions == None:
self.inclusions = EMData(self.alignment_file,1)
def set_events_mode(self,mode):
if not mode in self.events_mode_list:
print "error, unknown events mode", mode
return
else:
self.events_mode = mode
def closeEvent(self,event):
if self.inspector !=None: self.inspector.close()
if self.proj_class_viewer !=None: self.proj_class_viewer.close()
if self.proj_class_single !=None: self.proj_class_single.close()
if self.particle_viewer != None: self.particle_viewer.close()
get_application().close_specific(self)
self.emit(QtCore.SIGNAL("module_closed")) # this signal is
class EMClassPtclTool(QtGui.QWidget):
"""This class is a tab widget for inspecting particles within class-averages"""
def __init__(self,extrafiles=None):
QtGui.QWidget.__init__(self)
self.vbl = QtGui.QVBoxLayout(self)
self.extrafiles=extrafiles
# A listwidget for selecting which class-average file we're looking at
self.wclassfilel=QtGui.QLabel("Class-average File:")
self.vbl.addWidget(self.wclassfilel)
self.wfilesel=QtGui.QListWidget()
self.vbl.addWidget(self.wfilesel)
self.vbl.addSpacing(5)
# A widget containing the current particle filename, editable by the user
# If edited it will also impact set generation !
self.wptclfilel=QtGui.QLabel("Particle Data File:")
self.vbl.addWidget(self.wptclfilel)
self.wptclfile=QtGui.QComboBox(self)
self.vbl.addWidget(self.wptclfile)
self.vbl.addSpacing(5)
# Selection tools
self.wselectg=QtGui.QGroupBox("Class Selection",self)
self.wselectg.setFlat(False)
self.vbl.addWidget(self.wselectg)
self.vbl.addSpacing(5)
self.gbl0=QtGui.QGridLayout(self.wselectg)
self.wselallb=QtGui.QPushButton("All")
self.gbl0.addWidget(self.wselallb,0,0)
self.wselnoneb=QtGui.QPushButton("Clear")
self.gbl0.addWidget(self.wselnoneb,0,1)
self.wselrangeb=QtGui.QPushButton("Range")
self.gbl0.addWidget(self.wselrangeb,1,0)
self.wselinvertb=QtGui.QPushButton("Invert")
self.gbl0.addWidget(self.wselinvertb,0,2)
self.wsel3db=QtGui.QPushButton("From 3D")
self.gbl0.addWidget(self.wsel3db,1,2)
self.wprocessg=QtGui.QGroupBox("Process results",self)
self.wprocessg.setFlat(False)
self.vbl.addWidget(self.wprocessg)
self.vbl2=QtGui.QVBoxLayout(self.wprocessg)
self.wselused=CheckBox(None,"Included Ptcls",1,100)
self.vbl2.addWidget(self.wselused)
self.wselunused=CheckBox(None,"Excluded Ptcls",1,100)
self.vbl2.addWidget(self.wselunused)
# Mark particles in selected classes as bad
self.wmarkbut=QtGui.QPushButton("Mark as Bad")
self.vbl2.addWidget(self.wmarkbut)
# Mark particles in selected classes as good
self.wmarkgoodbut=QtGui.QPushButton("Mark as Good")
self.vbl2.addWidget(self.wmarkgoodbut)
# Make a new set from selected classes
self.wmakebut=QtGui.QPushButton("Make New Set")
self.vbl2.addWidget(self.wmakebut)
# self.wmakebut.setEnabled(False)
# Save list
self.wsavebut=QtGui.QPushButton("Save Particle List")
self.vbl2.addWidget(self.wsavebut)
# Save micrograph dereferenced lists
self.wsaveorigbut=QtGui.QPushButton("Save CCD-based List")
self.vbl2.addWidget(self.wsaveorigbut)
QtCore.QObject.connect(self.wfilesel,QtCore.SIGNAL("itemSelectionChanged()"),self.fileUpdate)
QtCore.QObject.connect(self.wptclfile,QtCore.SIGNAL("currentIndexChanged(int)"),self.ptclChange)
QtCore.QObject.connect(self.wselallb,QtCore.SIGNAL("clicked(bool)"),self.selAllClasses)
QtCore.QObject.connect(self.wselnoneb,QtCore.SIGNAL("clicked(bool)"),self.selNoClasses)
QtCore.QObject.connect(self.wselrangeb,QtCore.SIGNAL("clicked(bool)"),self.selRangeClasses)
QtCore.QObject.connect(self.wselinvertb,QtCore.SIGNAL("clicked(bool)"),self.selInvertClasses)
QtCore.QObject.connect(self.wsel3db,QtCore.SIGNAL("clicked(bool)"),self.sel3DClasses)
QtCore.QObject.connect(self.wmakebut,QtCore.SIGNAL("clicked(bool)"),self.makeNewSet)
QtCore.QObject.connect(self.wmarkbut,QtCore.SIGNAL("clicked(bool)"),self.markBadPtcl)
QtCore.QObject.connect(self.wmarkgoodbut,QtCore.SIGNAL("clicked(bool)"),self.markGoodPtcl)
QtCore.QObject.connect(self.wsavebut,QtCore.SIGNAL("clicked(bool)"),self.savePtclNum)
QtCore.QObject.connect(self.wsaveorigbut,QtCore.SIGNAL("clicked(bool)"),self.saveOrigPtclNum)
# View windows, one for class-averages, one for good particles and one for bad particles
self.vclasses=None
self.vgoodptcl=None
self.vbadptcl=None
self.updateFiles()
def makeNewSet(self,x):
"Makes a new particle set based on the selected class-averages"
setname=QtGui.QInputDialog.getText(None,"Set Name","Please specify the name for the set. If you specify an existing set, new particles will be added to the end")
if setname[1]==False : return
else: setname=setname[0]
if setname[-4:]!=".lst" : setname=setname+".lst"
if not "/" in setname : setname="sets/"+setname
lst=LSXFile(self.curPtclFile()) # lst file for dereferenceing
lstout=LSXFile(setname)
include=[]
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),self.wselunused.getValue()):
try :
orign,origfile,comment=lst.read(n) # the original file/number dereferenced from the LST file
except:
QtGui.QMessageBox.warning(self,"Error !","The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."%srcfile)
return
include.append((origfile,orign,comment)) # build a list so we can sort by frame
# write the new set
for i in sorted(include) : lstout.write(-1,i[1],i[0],i[2])
def markBadPtcl(self,x):
"Mark particles from the selected class-averages as bad in the set interface"
r=QtGui.QMessageBox.question(None,"Are you sure ?","WARNING: There is no undo for this operation. It will mark all particles associated with the selected class-averages as bad. Are you sure you want to proceed ?",QtGui.QMessageBox.Yes|QtGui.QMessageBox.Cancel)
if r==QtGui.QMessageBox.Cancel : return
lst=LSXFile(self.curPtclFile()) # lst file for dereferenceing
ptcls={} # dictionary keyed by original frame filename with list of selected particle #s
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),self.wselunused.getValue()):
try :
orign,origfile,comment=lst.read(n)
except:
QtGui.QMessageBox.warning(self,"Error !","The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."%srcfile)
return
try: ptcls[origfile].append(orign) # try to add to a list for an existing filename
except: ptcls[origfile]=[orign] # creates the list for this filename if it's new
#now mark the particles as bad
newbad=0
totbad=0
for origfile in ptcls:
js=js_open_dict(info_name(origfile)) # get the info dict for this file
try: sets=js["sets"]
except: sets={"bad_particles":[]}
try: badset=set(sets["bad_particles"])
except: badset=set()
try:
newset=list(set(ptcls[origfile])|badset)
sets["bad_particles"]=newset # update the set of bad particles for this image file
js["sets"]=sets
totbad+=len(badset)
newbad+=len(newset)-len(badset)
except:
print "Error setting bad particles in ",origfile
print newbad, " new particles marked as bad. Total of ",totbad," in affected micrographs"
def markGoodPtcl(self,x):
"Mark particles from the selected class-averages as good in the set interface"
r=QtGui.QMessageBox.question(None,"Are you sure ?","WARNING: There is no undo for this operation. It will un-mark all particles associated with the selected class-averages as bad. Are you sure you want to proceed ?",QtGui.QMessageBox.Yes|QtGui.QMessageBox.Cancel)
if r==QtGui.QMessageBox.Cancel : return
lst=LSXFile(self.curPtclFile()) # lst file for dereferenceing
ptcls={} # dictionary keyed by original frame filename with list of selected particle #s
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),self.wselunused.getValue()):
try :
orign,origfile,comment=lst.read(n)
except:
QtGui.QMessageBox.warning(self,"Error !","The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."%srcfile)
return
try: ptcls[origfile].append(orign) # try to add to a list for an existing filename
except: ptcls[origfile]=[orign] # creates the list for this filename if it's new
#now mark the particles as good
badafter=0
badbefore=0
for origfile in ptcls:
js=js_open_dict(info_name(origfile)) # get the info dict for this file
try:
badset=set(js["sets"]["bad_particles"])
js["sets"]["bad_particles"]=list(badset-set(ptcls[origfile])) # update the set of bad particles for this image file
except:
pass # since marking as good is the same as removing from the bad list, if there is no bad list, there is nothing to do
try: sets=js["sets"]
except: continue # if no current bad particles, nothing to mark good
try: badset=sets["bad_particles"]
except: continue
try:
newset=list(badset-set(ptcls[origfile]))
sets["bad_particles"]=newset # update the set of bad particles for this image file
js["sets"]=sets
badbefore+=len(badset)
badafter+=len(newset)
except:
continue
print badbefore," bad particles before processing, now ",badafter
def savePtclNum(self,x):
"Saves a list of particles from marked classes into a text file"
filename=QtGui.QInputDialog.getText(None,"Filename","Please enter a filename for the particle list. The file will contain the particle number (within the particle file) for each particle associated with a selected class-average.")
if filename[1]==False or filename[0]=="" : return
out=file(filename[0],"w")
for i in self.curPtclIter(self.wselused.getValue(),self.wselunused.getValue()): out.write("%d\n"%i)
out.close()
def saveOrigPtclNum(self,x):
"Saves a file containing micrograph-dereferenced particles"
filename=QtGui.QInputDialog.getText(None,"Filename","Please enter a filename for the particle list. The file will contain particle number and image file, one per line. Image files will be referenced back to the original per-CCD frame stacks.")
if filename[1]==False or filename[0]=="" : return
lst=LSXFile(self.curPtclFile()) # lst file for dereferenceing
include=[]
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),self.wselunused.getValue()):
try :
orign,origfile,comment=lst.read(n) # the original file/number dereferenced from the LST file
except:
QtGui.QMessageBox.warning(self,"Error !","The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."%srcfile)
return
include.append((origfile,orign,comment)) # build a list so we can sort by frame
# write the output file
out=file(filename,"w")
for i in sorted(include) : out.write("{}\t{}\n".format(i[1],i[0]))
out=None
def selAllClasses(self,x):
"Mark all classes as selected"
self.vclasses.all_set()
def selNoClasses(self,x):
"Clear selection"
self.vclasses.clear_set()
def selRangeClasses(self,x):
"Select a range of images (ask the user for the range)"
rng=QtGui.QInputDialog.getText(None,"Select Range","Enter the range of particle values as first-last (inclusive). Merges with existing selection.")
if rng[1]==False : return
try:
x0,x1=rng[0].split("-")
x0=int(x0)
x1=int(x1)+1
except:
QtGui.QMessageBox.warning(self,"Error !","Invalid range specified. Use: min-max")
return
self.vclasses.subset_set(range(x0,x1))
def selInvertClasses(self,x):
"Inverts the current selection set"
self.vclasses.invert_set()
def sel3DClasses(self,x):
"Select a range of images based on those used in a 3-D reconstruction associated with this classes file. Removes current selection first."
f=self.curFile()
if not '#classes_' in f :
QtGui.QMessageBox.warning(self,"Error !","A classes_xx file from a refine_xx directory is not currently selected")
return
# construct the path to the threed_xx file
num=f.split("_")[-1]
pre=f.split("#")[0]
d3path="%s#threed_%s"%(pre,num)
try:
a=EMData(d3path,0,True)
goodptcl=a["threed_ptcl_idxs"]
except:
QtGui.QMessageBox.warning(self,"Error !","Cannot read classes from "+d3path)
return
self.vclasses.clear_set()
self.vclasses.subset_set(goodptcl)
def ptclChange(self,value):
"Called when a change of particle data file occurs to zero out the display"
try:
self.vgoodptcl.set_data(None)
self.vbadptcl.set_data(None)
except:
pass
def updateFiles(self):
"Updates the list of classes files"
subdir=sorted([i for i in os.listdir(e2getcwd()) if "r2d_" in i or "relion2d_" in i or "refine_" in i or "multi_" in i])
for d in subdir:
try: dbs=os.listdir(d)
except: continue
dbs.sort()
for db in dbs:
if "classes_" in db or "allrefs_" in db :
self.wfilesel.addItem("%s/%s"%(d,db))
for f in self.extrafiles:
self.wfilesel.addItem(f)
dbs=os.listdir("sets")
dbs.sort()
for db in dbs:
self.wptclfile.addItem("sets/"+db)
def curPtclIter(self,included=True,excluded=True):
"This is a generator function which yields n (in self.curPtclFile()) for all particles associated with selected classes"
for ci in self.curSet():
try :
c=EMData(self.curFile(),ci,True) # read header for current class average
if included :
incl=c["class_ptcl_idxs"]
if isinstance(incl,int) : incl=[incl] # This should not happen, but seems to sometimes for some reason
for i in incl:
yield(i)
if excluded and c.has_attr("exc_class_ptcl_idxs"):
excl=c["exc_class_ptcl_idxs"]
if isinstance(excl,int) : excl=[excl] # This should not happen, but seems to sometimes for some reason
for i in excl:
yield(i)
except:
print "Problem with class %d (%s). Skipping"%(ci,self.curFile())
traceback.print_exc()
continue
def curFile(self):
"return the currently selected file as a readable path"
return str(self.wfilesel.item(self.wfilesel.currentRow()).text()) # text of the currently selected item
def curSet(self):
"return a set (integers) of the currently selected class-averages"
return self.vclasses.get_set("evalptcl")
def curPtclFile(self):
"return the particle file associated with the currently selected classes file"
return str(self.wptclfile.currentText()) # text of the currently selected item
def fileUpdate(self):
"Called when the user selects a file from the list or need to completely refresh display"
QtGui.qApp.setOverrideCursor(Qt.BusyCursor)
if self.vclasses==None :
self.vclasses=EMImageMXWidget()
self.vclasses.set_mouse_mode("App")
QtCore.QObject.connect(self.vclasses,QtCore.SIGNAL("mx_image_selected"),self.classSelect)
QtCore.QObject.connect(self.vclasses,QtCore.SIGNAL("mx_image_double"),self.classDouble)
self.vclasses.set_title("Classes")
# self.classes=EMData.read_images(self.curFile())
self.vclasses.set_data(self.curFile(),self.curFile())
# self.vclasses.set_single_active_set("selected") # This makes the 'set' representing the selected class-averages current
self.vclasses.set_mouse_mode("App")
self.vclasses.enable_set("evalptcl",[])
# This makes sure the particle file is in the list of choices and is selected
try:
ptclfile=EMData(self.curFile(),0,True)["class_ptcl_src"]
i=self.wptclfile.findText(ptclfile)
if i==-1 :
self.wptclfile.insertItem(0,ptclfile)
self.wptclfile.setCurrentIndex(0)
else:
self.wptclfile.setCurrentIndex(i)
except:
QtGui.QMessageBox.warning(self,"Error !","This image does not appear to be a class average. (No class_ptcl_src, etc.)")
ptclfile="None"
# Make sure our display widgets exist
if self.vgoodptcl==None :
self.vgoodptcl=EMImageMXWidget()
self.vgoodptcl.set_title("Included Particles")
if self.vbadptcl==None :
self.vbadptcl=EMImageMXWidget()
self.vbadptcl.set_title("Excluded Particles")
self.vclasses.show()
self.vgoodptcl.show()
self.vbadptcl.show()
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
def classSelect(self,event,lc):
"Single clicked class particle. lc=(img#,x,y,image_dict)"
QtGui.qApp.setOverrideCursor(Qt.BusyCursor)
ptclfile=self.curPtclFile()
try:
ptclgood=lc[3]["class_ptcl_idxs"]
self.vgoodptcl.set_data(EMData.read_images(ptclfile,ptclgood))
except:
QtGui.QMessageBox.warning(self,"Error !","This image does not appear to be a class average. (No class_ptcl_src, etc.)")
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
return
try:
ptclbad=lc[3]["exc_class_ptcl_idxs"]
self.vbadptcl.set_data(EMData.read_images(ptclfile,ptclbad))
except:
ptclbad=[]
self.vbadptcl.set_data(None)
self.vgoodptcl.show()
self.vbadptcl.show()
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
def classDouble(self,event,lc):
self.vclasses.image_set_associate(lc[0],update_gl=True)
def closeEvent(self,event):
try :
self.vclasses.commit_sets()
self.vclasses.close()
except: pass
try : self.vgoodptcl.close()
except: pass
try : self.vbadptcl.close()
except: pass
QtGui.QWidget.closeEvent(self, event)
def __new__(cls,data=None,old=None,app=None,force_2d=False,force_plot=False,filename="",replace=True):
"""This will create a new EMImage* object depending on the type of 'data'. If
old= is provided, and of the appropriate type, it will be used rather than creating
a new instance.
"""
if isinstance(data,EMData) and data.get_size()==0: raise RuntimeError("Can not display an EMData object that has no pixels")
from EMAN2 import remove_directories_from_name
if force_plot and force_2d:
# ok this sucks but it suffices for the time being
print "Error, the force_plot and force_2d options are mutually exclusive"
return None
if force_plot or (isinstance(data,EMData) and data.get_zsize()==1 and data.get_ysize()==1):
from emplot2d import EMPlot2DWidget
if old:
if isinstance(old,EMPlot2DWidget) :
old.set_data(data,remove_directories_from_name(filename),replace)
return old
widget = EMPlot2DWidget(application=app)
widget.set_data(data,remove_directories_from_name(filename),replace)
return widget
elif force_2d or (isinstance(data,EMData) and data.get_zsize()==1):
from emimage2d import EMImage2DWidget
if old:
if isinstance(old,EMImage2DWidget) :
old.set_data(data,filename)
return old
widget = EMImage2DWidget(application=app)
widget.set_data(data,filename)
return widget
elif isinstance(data,EMData):
if isinstance(old,EMScene3D): widget = old
else: widget = EMScene3D()
data = EMDataItem3D(data, transform=Transform())
#data.setSelectedItem(True)
isosurface = EMIsosurface(data, transform=Transform())
widget.insertNewNode(os.path.basename(filename), data, parentnode=widget)
widget.insertNewNode("Iso", isosurface, parentnode=data)
return widget
elif isinstance(data,list) and isinstance(data[0],EMData):
from emimagemx import EMImageMXWidget
if old:
if isinstance(old,EMImageMXWidget) :
old.set_data(data,filename)
return old
widget = EMImageMXWidget(application=app)
widget.set_data(data,filename)
return widget
elif isinstance(data,list):
from emplot3d import EMPlot3DWidgetNew
if (isinstance(data[0],list) or isinstance(data[0],tuple)) and len(data) > 2:
if old:
if isinstance(old,EMPlot3DWidgetNew) :
old.set_data(data,remove_directories_from_name(filename),replace)
return old
widget = EMPlot3DWidgetNew()
widget.set_data(data,remove_directories_from_name(filename),replace)
return widget
else:
from emplot2d import EMPlot2DWidget
if old:
if isinstance(old,EMPlot2DWidget) :
old.set_data(data,remove_directories_from_name(filename),replace)
return old
widget = EMPlot2DWidget(application=app)
widget.set_data(data,remove_directories_from_name(filename),replace)
return widget
else:
raise Exception,"data must be a single EMData object or a list of EMData objects"
class EMClassPtclTool(QtGui.QWidget):
"""This class is a tab widget for inspecting particles within class-averages"""
def __init__(self, extrafiles=None):
QtGui.QWidget.__init__(self)
self.vbl = QtGui.QVBoxLayout(self)
self.extrafiles = extrafiles
# A listwidget for selecting which class-average file we're looking at
self.wclassfilel = QtGui.QLabel("Class-average File:")
self.vbl.addWidget(self.wclassfilel)
self.wfilesel = QtGui.QListWidget()
self.vbl.addWidget(self.wfilesel)
self.vbl.addSpacing(5)
# A widget containing the current particle filename, editable by the user
# If edited it will also impact set generation !
self.wptclfilel = QtGui.QLabel("Particle Data File:")
self.vbl.addWidget(self.wptclfilel)
self.wptclfile = QtGui.QComboBox(self)
self.vbl.addWidget(self.wptclfile)
self.vbl.addSpacing(5)
# Selection tools
self.wselectg = QtGui.QGroupBox("Class Selection", self)
self.wselectg.setFlat(False)
self.vbl.addWidget(self.wselectg)
self.vbl.addSpacing(5)
self.gbl0 = QtGui.QGridLayout(self.wselectg)
self.wselallb = QtGui.QPushButton("All")
self.gbl0.addWidget(self.wselallb, 0, 0)
self.wselnoneb = QtGui.QPushButton("Clear")
self.gbl0.addWidget(self.wselnoneb, 0, 1)
self.wselrangeb = QtGui.QPushButton("Range")
self.gbl0.addWidget(self.wselrangeb, 1, 0)
self.wselinvertb = QtGui.QPushButton("Invert")
self.gbl0.addWidget(self.wselinvertb, 0, 2)
self.wsel3db = QtGui.QPushButton("From 3D")
self.gbl0.addWidget(self.wsel3db, 1, 2)
self.wprocessg = QtGui.QGroupBox("Process results", self)
self.wprocessg.setFlat(False)
self.vbl.addWidget(self.wprocessg)
self.vbl2 = QtGui.QVBoxLayout(self.wprocessg)
self.wselused = CheckBox(None, "Included Ptcls", 1, 100)
self.vbl2.addWidget(self.wselused)
self.wselunused = CheckBox(None, "Excluded Ptcls", 1, 100)
self.vbl2.addWidget(self.wselunused)
# Mark particles in selected classes as bad
self.wmarkbut = QtGui.QPushButton("Mark as Bad")
self.vbl2.addWidget(self.wmarkbut)
# Mark particles in selected classes as good
self.wmarkgoodbut = QtGui.QPushButton("Mark as Good")
self.vbl2.addWidget(self.wmarkgoodbut)
# Make a new set from selected classes
self.wmakebut = QtGui.QPushButton("Make New Set")
self.vbl2.addWidget(self.wmakebut)
# self.wmakebut.setEnabled(False)
# Save list
self.wsavebut = QtGui.QPushButton("Save Particle List")
self.vbl2.addWidget(self.wsavebut)
# Save micrograph dereferenced lists
self.wsaveorigbut = QtGui.QPushButton("Save CCD-based List")
self.vbl2.addWidget(self.wsaveorigbut)
QtCore.QObject.connect(self.wfilesel,
QtCore.SIGNAL("itemSelectionChanged()"),
self.fileUpdate)
QtCore.QObject.connect(self.wptclfile,
QtCore.SIGNAL("currentIndexChanged(int)"),
self.ptclChange)
QtCore.QObject.connect(self.wselallb, QtCore.SIGNAL("clicked(bool)"),
self.selAllClasses)
QtCore.QObject.connect(self.wselnoneb, QtCore.SIGNAL("clicked(bool)"),
self.selNoClasses)
QtCore.QObject.connect(self.wselrangeb, QtCore.SIGNAL("clicked(bool)"),
self.selRangeClasses)
QtCore.QObject.connect(self.wselinvertb,
QtCore.SIGNAL("clicked(bool)"),
self.selInvertClasses)
QtCore.QObject.connect(self.wsel3db, QtCore.SIGNAL("clicked(bool)"),
self.sel3DClasses)
QtCore.QObject.connect(self.wmakebut, QtCore.SIGNAL("clicked(bool)"),
self.makeNewSet)
QtCore.QObject.connect(self.wmarkbut, QtCore.SIGNAL("clicked(bool)"),
self.markBadPtcl)
QtCore.QObject.connect(self.wmarkgoodbut,
QtCore.SIGNAL("clicked(bool)"),
self.markGoodPtcl)
QtCore.QObject.connect(self.wsavebut, QtCore.SIGNAL("clicked(bool)"),
self.savePtclNum)
QtCore.QObject.connect(self.wsaveorigbut,
QtCore.SIGNAL("clicked(bool)"),
self.saveOrigPtclNum)
# View windows, one for class-averages, one for good particles and one for bad particles
self.vclasses = None
self.vgoodptcl = None
self.vbadptcl = None
self.updateFiles()
def makeNewSet(self, x):
"Makes a new particle set based on the selected class-averages"
setname = QtGui.QInputDialog.getText(
None, "Set Name",
"Please specify the name for the set. If you specify an existing set, new particles will be added to the end"
)
if setname[1] == False: return
else: setname = setname[0]
if setname[-4:] != ".lst": setname = setname + ".lst"
if not "/" in setname: setname = "sets/" + setname
lst = LSXFile(self.curPtclFile()) # lst file for dereferenceing
lstout = LSXFile(setname)
include = []
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),
self.wselunused.getValue()):
try:
orign, origfile, comment = lst.read(
n
) # the original file/number dereferenced from the LST file
except:
QtGui.QMessageBox.warning(
self, "Error !",
"The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."
% srcfile)
return
include.append((origfile, orign,
comment)) # build a list so we can sort by frame
# write the new set
for i in sorted(include):
lstout.write(-1, i[1], i[0], i[2])
def markBadPtcl(self, x):
"Mark particles from the selected class-averages as bad in the set interface"
r = QtGui.QMessageBox.question(
None, "Are you sure ?",
"WARNING: There is no undo for this operation. It will mark all particles associated with the selected class-averages as bad. Are you sure you want to proceed ?",
QtGui.QMessageBox.Yes | QtGui.QMessageBox.Cancel)
if r == QtGui.QMessageBox.Cancel: return
lst = LSXFile(self.curPtclFile()) # lst file for dereferenceing
ptcls = {
} # dictionary keyed by original frame filename with list of selected particle #s
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),
self.wselunused.getValue()):
try:
orign, origfile, comment = lst.read(n)
except:
QtGui.QMessageBox.warning(
self, "Error !",
"The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."
% srcfile)
return
try:
ptcls[origfile].append(
orign) # try to add to a list for an existing filename
except:
ptcls[origfile] = [
orign
] # creates the list for this filename if it's new
#now mark the particles as bad
newbad = 0
totbad = 0
for origfile in ptcls:
js = js_open_dict(
info_name(origfile)) # get the info dict for this file
try:
sets = js["sets"]
except:
sets = {"bad_particles": []}
try:
badset = set(sets["bad_particles"])
except:
badset = set()
try:
newset = list(set(ptcls[origfile]) | badset)
sets[
"bad_particles"] = newset # update the set of bad particles for this image file
js["sets"] = sets
totbad += len(badset)
newbad += len(newset) - len(badset)
except:
print "Error setting bad particles in ", origfile
js_close_dict(info_name(origfile))
print newbad, " new particles marked as bad. Total of ", totbad, " in affected micrographs"
def markGoodPtcl(self, x):
"Mark particles from the selected class-averages as good in the set interface"
r = QtGui.QMessageBox.question(
None, "Are you sure ?",
"WARNING: There is no undo for this operation. It will un-mark all particles associated with the selected class-averages as bad. Are you sure you want to proceed ?",
QtGui.QMessageBox.Yes | QtGui.QMessageBox.Cancel)
if r == QtGui.QMessageBox.Cancel: return
lst = LSXFile(self.curPtclFile()) # lst file for dereferenceing
ptcls = {
} # dictionary keyed by original frame filename with list of selected particle #s
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),
self.wselunused.getValue()):
try:
orign, origfile, comment = lst.read(n)
except:
QtGui.QMessageBox.warning(
self, "Error !",
"The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."
% srcfile)
return
try:
ptcls[origfile].append(
orign) # try to add to a list for an existing filename
except:
ptcls[origfile] = [
orign
] # creates the list for this filename if it's new
#now mark the particles as good
badafter = 0
badbefore = 0
for origfile in ptcls:
js = js_open_dict(
info_name(origfile)) # get the info dict for this file
try:
badset = set(js["sets"]["bad_particles"])
js["sets"]["bad_particles"] = list(
badset - set(ptcls[origfile])
) # update the set of bad particles for this image file
except:
pass # since marking as good is the same as removing from the bad list, if there is no bad list, there is nothing to do
try:
sets = js["sets"]
except:
continue # if no current bad particles, nothing to mark good
try:
badset = sets["bad_particles"]
except:
continue
try:
newset = list(badset - set(ptcls[origfile]))
sets[
"bad_particles"] = newset # update the set of bad particles for this image file
js["sets"] = sets
badbefore += len(badset)
badafter += len(newset)
except:
continue
print badbefore, " bad particles before processing, now ", badafter
def savePtclNum(self, x):
"Saves a list of particles from marked classes into a text file"
filename = QtGui.QInputDialog.getText(
None, "Filename",
"Please enter a filename for the particle list. The file will contain the particle number (within the particle file) for each particle associated with a selected class-average."
)
if filename[1] == False or filename[0] == "": return
out = file(filename[0], "w")
for i in self.curPtclIter(self.wselused.getValue(),
self.wselunused.getValue()):
out.write("%d\n" % i)
out.close()
def saveOrigPtclNum(self, x):
"Saves a file containing micrograph-dereferenced particles"
filename = QtGui.QInputDialog.getText(
None, "Filename",
"Please enter a filename for the particle list. The file will contain particle number and image file, one per line. Image files will be referenced back to the original per-CCD frame stacks."
)
if filename[1] == False or filename[0] == "": return
lst = LSXFile(self.curPtclFile()) # lst file for dereferenceing
include = []
# iterate over each particle from each marked class-average
for n in self.curPtclIter(self.wselused.getValue(),
self.wselunused.getValue()):
try:
orign, origfile, comment = lst.read(
n
) # the original file/number dereferenced from the LST file
except:
QtGui.QMessageBox.warning(
self, "Error !",
"The data_source '%s' does not follow EMAN2.1 project conventions. Cannot find raw particles for set."
% srcfile)
return
include.append((origfile, orign,
comment)) # build a list so we can sort by frame
# write the output file
out = file(filename, "w")
for i in sorted(include):
out.write("{}\t{}\n".format(i[1], i[0]))
out = None
def selAllClasses(self, x):
"Mark all classes as selected"
self.vclasses.all_set()
def selNoClasses(self, x):
"Clear selection"
self.vclasses.clear_set()
def selRangeClasses(self, x):
"Select a range of images (ask the user for the range)"
rng = QtGui.QInputDialog.getText(
None, "Select Range",
"Enter the range of particle values as first-last (inclusive). Merges with existing selection."
)
if rng[1] == False: return
try:
x0, x1 = rng[0].split("-")
x0 = int(x0)
x1 = int(x1) + 1
except:
QtGui.QMessageBox.warning(self, "Error !",
"Invalid range specified. Use: min-max")
return
self.vclasses.subset_set(range(x0, x1))
def selInvertClasses(self, x):
"Inverts the current selection set"
self.vclasses.invert_set()
def sel3DClasses(self, x):
"Select a range of images based on those used in a 3-D reconstruction associated with this classes file. Removes current selection first."
f = self.curFile()
if not '#classes_' in f:
QtGui.QMessageBox.warning(
self, "Error !",
"A classes_xx file from a refine_xx directory is not currently selected"
)
return
# construct the path to the threed_xx file
num = f.split("_")[-1]
pre = f.split("#")[0]
d3path = "%s#threed_%s" % (pre, num)
try:
a = EMData(d3path, 0, True)
goodptcl = a["threed_ptcl_idxs"]
except:
QtGui.QMessageBox.warning(self, "Error !",
"Cannot read classes from " + d3path)
return
self.vclasses.clear_set()
self.vclasses.subset_set(goodptcl)
def ptclChange(self, value):
"Called when a change of particle data file occurs to zero out the display"
try:
self.vgoodptcl.set_data(None)
self.vbadptcl.set_data(None)
except:
pass
def updateFiles(self):
"Updates the list of classes files"
subdir = sorted([
i for i in os.listdir(e2getcwd())
if "r2d_" in i or "relion2d_" in i or "refine_" in i
or "multi_" in i or "multinoali_" in i
])
for d in subdir:
try:
dbs = os.listdir(d)
except:
continue
dbs.sort()
for db in dbs:
if "classes_" in db or "allrefs_" in db:
self.wfilesel.addItem("%s/%s" % (d, db))
for f in self.extrafiles:
self.wfilesel.addItem(f)
dbs = os.listdir("sets")
dbs.sort()
for db in dbs:
self.wptclfile.addItem("sets/" + db)
def curPtclIter(self, included=True, excluded=True):
"This is a generator function which yields n (in self.curPtclFile()) for all particles associated with selected classes"
for ci in self.curSet():
try:
c = EMData(self.curFile(), ci,
True) # read header for current class average
if included:
incl = c["class_ptcl_idxs"]
if isinstance(incl, int):
incl = [
incl
] # This should not happen, but seems to sometimes for some reason
for i in incl:
yield (i)
if excluded and c.has_attr("exc_class_ptcl_idxs"):
excl = c["exc_class_ptcl_idxs"]
if isinstance(excl, int):
excl = [
excl
] # This should not happen, but seems to sometimes for some reason
for i in excl:
yield (i)
except:
print "Problem with class %d (%s). Skipping" % (ci,
self.curFile())
traceback.print_exc()
continue
def curFile(self):
"return the currently selected file as a readable path"
return str(self.wfilesel.item(self.wfilesel.currentRow()).text()
) # text of the currently selected item
def curSet(self):
"return a set (integers) of the currently selected class-averages"
return self.vclasses.get_set("evalptcl")
def curPtclFile(self):
"return the particle file associated with the currently selected classes file"
return str(self.wptclfile.currentText()
) # text of the currently selected item
def fileUpdate(self):
"Called when the user selects a file from the list or need to completely refresh display"
QtGui.qApp.setOverrideCursor(Qt.BusyCursor)
if self.vclasses == None:
self.vclasses = EMImageMXWidget()
self.vclasses.set_mouse_mode("App")
QtCore.QObject.connect(self.vclasses,
QtCore.SIGNAL("mx_image_selected"),
self.classSelect)
QtCore.QObject.connect(self.vclasses,
QtCore.SIGNAL("mx_image_double"),
self.classDouble)
self.vclasses.set_title("Classes")
# self.classes=EMData.read_images(self.curFile())
self.vclasses.set_data(self.curFile(), self.curFile())
# self.vclasses.set_single_active_set("selected") # This makes the 'set' representing the selected class-averages current
self.vclasses.set_mouse_mode("App")
self.vclasses.enable_set("evalptcl", [])
# This makes sure the particle file is in the list of choices and is selected
try:
ptclfile = EMData(self.curFile(), 0, True)["class_ptcl_src"]
i = self.wptclfile.findText(ptclfile)
if i == -1:
self.wptclfile.insertItem(0, ptclfile)
self.wptclfile.setCurrentIndex(0)
else:
self.wptclfile.setCurrentIndex(i)
except:
QtGui.QMessageBox.warning(
self, "Error !",
"This image does not appear to be a class average. (No class_ptcl_src, etc.)"
)
ptclfile = "None"
# Make sure our display widgets exist
if self.vgoodptcl == None:
self.vgoodptcl = EMImageMXWidget()
self.vgoodptcl.set_title("Included Particles")
if self.vbadptcl == None:
self.vbadptcl = EMImageMXWidget()
self.vbadptcl.set_title("Excluded Particles")
self.vclasses.show()
self.vgoodptcl.show()
self.vbadptcl.show()
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
def classSelect(self, event, lc):
"Single clicked class particle. lc=(img#,x,y,image_dict)"
QtGui.qApp.setOverrideCursor(Qt.BusyCursor)
ptclfile = self.curPtclFile()
try:
ptclgood = lc[3]["class_ptcl_idxs"]
self.vgoodptcl.set_data(EMData.read_images(ptclfile, ptclgood))
except:
QtGui.QMessageBox.warning(
self, "Error !",
"This image does not appear to be a class average. (No class_ptcl_src, etc.)"
)
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
return
try:
ptclbad = lc[3]["exc_class_ptcl_idxs"]
self.vbadptcl.set_data(EMData.read_images(ptclfile, ptclbad))
except:
ptclbad = []
self.vbadptcl.set_data(None)
self.vgoodptcl.show()
self.vbadptcl.show()
QtGui.qApp.setOverrideCursor(Qt.ArrowCursor)
def classDouble(self, event, lc):
self.vclasses.image_set_associate(lc[0], update_gl=True)
def closeEvent(self, event):
try:
self.vclasses.commit_sets()
self.vclasses.close()
except:
pass
try:
self.vgoodptcl.close()
except:
pass
try:
self.vbadptcl.close()
except:
pass
QtGui.QWidget.closeEvent(self, event)
class EMEulerExplorer(EM3DSymModel,Animator):
def mousePressEvent(self,event):
if self.events_mode == "inspect":
self.current_hit = self.get_hit(event)
if self.current_hit == None:
EM3DSymModel.mousePressEvent(self,event)
else:
EM3DSymModel.mousePressEvent(self,event)
def mouseReleaseEvent(self,event):
if self.events_mode == "inspect":
if self.current_hit != None:
self.updateGL() # there needs to be a clear or something in order for the picking to work. This is bit of hack but our rendering function doesn't take long anyhow
hit = self.get_hit(event)
if hit == self.current_hit:
self.emit(QtCore.SIGNAL("point_selected"),self.current_hit,event)
else:
#EM3DSymModel.mouseReleaseEvent(self,event)
EM3DModel.mouseReleaseEvent(self, event) #behavior in EM3DSymModel is not what we want (needed in sibling classes?)
self.current_hit = None
else:
#EM3DSymModel.mouseReleaseEvent(self,event)
EM3DModel.mouseReleaseEvent(self, event) #behavior in EM3DSymModel is not what we want (needed in sibling classes?)
def mouseMoveEvent(self,event):
if self.events_mode == "inspect" and self.current_hit:
pass
else:
EM3DSymModel.mouseMoveEvent(self,event)
def get_hit(self,event):
v = self.vdtools.wview.tolist()
self.get_gl_widget().makeCurrent() # prevents a stack underflow
# x = event.x()
# y = v[-1]-event.y()
# glClear(GL_COLOR_BUFFER_BIT | GL_DEPTH_BUFFER_BIT | GL_STENCIL_BUFFER_BIT )
# vals = self.render(color_picking=True)
# glFlush()
# vv = glReadPixels(x,y,1,1,GL_RGB,GL_FLOAT)
# reslt = Vec3f(float(vv[0][0][0]),float(vv[0][0][1]),float(vv[0][0][2]))
# for i,val in enumerate(vals):
## print val,reslt,(reslt-val).length(),vv[0][0]
# if (reslt-val).length() < 0.01:
# print i
## print (reslt-val).length()
# return i
# print vv
#
# the problem with this approach is that depth testing is not part of picking
sb = [0 for i in xrange(0,512)]
glSelectBuffer(512)
glRenderMode(GL_SELECT)
glInitNames()
glMatrixMode(GL_PROJECTION)
glPushMatrix()
glLoadIdentity()
gluPickMatrix(event.x(),v[-1]-event.y(),5,5,v)
self.get_gl_widget().load_perspective()
glMatrixMode(GL_MODELVIEW)
glInitNames()
self.render()
glMatrixMode(GL_PROJECTION)
glPopMatrix()
glMatrixMode(GL_MODELVIEW)
glFlush()
intersection = None
hits = list(glRenderMode(GL_RENDER))
for hit in hits:
a,b,c=hit
if len(c) > 0:
intersection = c[0]-1
break
return intersection
def keyPressEvent(self,event):
if event.key() == Qt.Key_F1:
self.display_web_help("http://blake.bcm.edu/emanwiki/EMAN2/Programs/e2eulerxplor")
elif event.key() == Qt.Key_F :
if self.flatten>0 : self.flatten=0.0
else: self.flatten=1.0
self.generate_current_display_list(True)
self.updateGL()
else:
EM3DSymModel.keyPressEvent(self,event)
def __init__(self, gl_widget=None, auto=True,sparse_mode=False, file_name = "", read_from=None):
self.current_hit = None
self.events_mode_list = ["navigate", "inspect"]
self.events_mode = self.events_mode_list[1]
self.init_lock = True # a lock indicated that we are still in the __init__ function
self.au_data = None # This will be a dictionary, keys will be refinement directories, values will be something like available iterations for visual study
if len(read_from)==0:
read_from=None
self.readfrom=read_from
if self.readfrom:
file_name=""
auto=False
datalst=[]
for rr in self.readfrom:
datalst.append([rr,None, None, None,rr])
self.au_data={"data":datalst}
if auto: # this a flag that tells the eulerxplorer to search for refinement data and automatically add elements to the inspector, if so
self.gen_refinement_data()
EM3DSymModel.__init__(self, gl_widget, eulerfilename=file_name)
Animator.__init__(self)
self.height_scale = 8.0 # This is a value used in EM3DSymModel which scales the height of the displayed cylinders - I made it 8 because it seemed fine. The user can change it anyhow
self.projection_file = None # This is a string - the name of the projection images file
self.average_file = None # This is a string - the name of the class averages file
self.proj_class_viewer = None # This will be an EMImageMXWidget that shows the class and/or projection
self.particle_viewer = None # This will be an EMImageMXWidget that shows the particles in a class
self.clsdb = None # I think this will become redundant - it used to be the old database that stores which particles are in a class, but now that's stored in the header
self.particle_file = None # This will be a string - the name of the file that has the particle files in it. This might be made redundant with the new approach
self.alignment_file = None # This will be a string - the name of the file containing the alignment parameters - this is essential if you we want to show the aligned particles
self.refine_dir = None # This will be a string - the name of the current refinement directory that is being studied
self.dx = None # This is an EMData object storing the x shifts of the alignments for all particles. Generated by e2classaverage
self.dy = None # This is an EMData object storing the y shifts of the alignments for all particles. Generated by e2classaverage
self.da = None# This is an EMData object storing the angle of the alignments for all particles. Generated by e2classaverage
self.dflip = None # This is an EMData object storing whether or not tthe alignment involved a flip, for all particles. Generated by e2classaverage
self.classes = None # This is an EMData object storing which class(es) a particle belongs to. Generated by e2classaverage
self.inclusions = None # This is and EMDAta storing a boolean that indicates the particle was actually included in the final average. Generated by e2classaverage
self.average = None # This the class average itself, an EMData object
self.projection = None # This is the projection itelse, an EMData object
self.class_idx = None # This is the idx of the current class being studied in the interface
self.previous_len = -1 # To keep track of the number of class averages that were previously viewable. This helps to make sure we can switch to the same class average in the context of a different refinement iteration
self.mirror_eulers = False
if sparse_mode:
self.mirror_eulers = True # If True the drawn Eulers are are also rendered on the opposite side of the sphere - see EM3DSymModel.make_sym_dl_lis
# Grab the symmetry from the workflow database if possible
sym = "c1"
if js_check_dict("refine_01/0_refine_parms.json"):
try: sym = str(js_open_dict("refine_01/0_refine_parms.json")["sym"])
except: pass
# Have to tell the EM3DSymModel that there is a new sym
self.set_symmetry(sym)
# this object will have
if self.au_data != None:
combo_entries = self.au_data.keys()
combo_entries.sort()
combo_entries.reverse()
if len(combo_entries) > 0:
au = combo_entries[0]
cls = self.au_data[au][0][0]
self.au_selected(au,cls)
self.mirror_eulers = True
self.init_lock = False
self.force_update=True # Force a display udpdate in EMImage3DSymModule
QtCore.QObject.connect(self, QtCore.SIGNAL("point_selected"), self.au_point_selected)
def __del__(self):
EM3DSymModel.__del__(self) # this is here for documentation purposes - beware that the del function is important
def initializeGL(self):
glEnable(GL_NORMALIZE)
def generate_current_display_list(self,force=False):
'''
Redefinition of EMImage3DSymModule.generate_current_display_list
'''
if self.init_lock: return 0
if self.au_data == None or len(self.au_data) == 0:
EM3DSymModel.generate_current_display_list(self,force)
self.init_basic_shapes()
if self.nomirror == True : val = 0
else: val = 1
self.trace_great_arcs(self.sym_object.get_asym_unit_points(val))
self.trace_great_triangles(val)
self.eulers = self.specified_eulers
if self.eulers == None: return 0
# if not self.colors_specified: self.point_colors = []
# else: self.point_colors = self.specified_colors
# self.points = []
# for i in self.eulers:
# p = i.transpose()*Vec3f(0,0,self.radius)
# self.points.append(p)
# if not self.colors_specified: self.point_colors.append((0.34615, 0.3143, 0.0903,1))
self.make_sym_dl_list(self.eulers)
return 1
def get_data_dims(self):
return (2*self.radius,2*self.radius,2*self.radius)
def width(self): return 2*self.radius
def height(self): return 2*self.radius
def depth(self): return 2*self.radius
def gen_refinement_data(self):
dirs,files = get_files_and_directories()
dirs.sort()
for i in range(len(dirs)-1,-1,-1):
if dirs[i][:7] != "refine_" and dirs[i][:6]!="multi_" and dirs[i][:11]!="multinoali_":
dirs.pop(i)
else:
try: int(dirs[i][7:])
except: dirs.pop(i)
self.dirs = dirs
print(dirs)
self.au_data = {}
for dir in self.dirs:
d = self.check_refine_db_dir(dir)
if len(d) != 0 and len(d[dir]) != 0: self.au_data.update(d)
def check_refine_db_dir(self,dir,s1="classes",s2=None,s3="cls_result",s4="threed",s5="projections"):
# s2 used to be class_indices
names = [s1,s2,s3,s4,s5]
data = {}
data[dir] = []
register_js_name = "{}/0_refine_parms.json".format(dir)
files=os.listdir(dir)
try:
nums=[int(i[7:9]) for i in files if "threed" in i and "even" not in i and "odd" not in i]
maxnum=max(nums)
except :
print("Nothing in ",dir)
return {}
for i in xrange(1,maxnum+1):
exte="_{:02d}_even.hdf".format(i)
exto="_{:02d}_odd.hdf".format(i)
data[dir].append([sadd(dir,s1,exte),sadd(dir,s2,exte),sadd(dir,s3,exte),sadd(dir,s4,exte),sadd(dir,s5,exte)])
data[dir].append([sadd(dir,s1,exto),sadd(dir,s2,exto),sadd(dir,s3,exto),sadd(dir,s4,exto),sadd(dir,s5,exto)])
return data
def set_projection_file(self,projection_file): self.projection_file = projection_file
def get_inspector(self):
if not self.inspector :
if (self.au_data == None or len(self.au_data) == 0) and self.mirror_eulers == False: #self.mirror_eulers thing is a little bit of a hack, it's tied to the sparse_mode flag in the init function, which is used by euler_display in EMAN2.py
self.inspector=EMAsymmetricUnitInspector(self,True,True)
else:
self.inspector=EMAsymmetricUnitInspector(self)
QtCore.QObject.connect(self.inspector,QtCore.SIGNAL("au_selected"),self.au_selected)
return self.inspector
def au_selected(self,refine_dir,cls):
self.refine_dir = refine_dir
get_application().setOverrideCursor(Qt.BusyCursor)
data = []
for d in self.au_data[refine_dir]:
if d[0] == cls:
data = d;
break
if len(data) == 0:
error("error, no data for %s %s, returning" %(refine_dir,cls))
# print "error, no data for",au,cls,"returning"
self.events_handlers["inspect"].reset()
get_application().setOverrideCursor(Qt.ArrowCursor)
return
if not self.readfrom:
try :
self.particle_file=js_open_dict(refine_dir+"/0_refine_parms.json")["input"]
except:
error("No data in "+refine_dir )
self.events_handlers["inspect"].reset()
get_application().setOverrideCursor(Qt.ArrowCursor)
return
self.average_file = cls
self.projection_file = data[4]
self.alignment_file = data[2]
self.clsdb = data[1]
self.dx = None
self.dy = None
self.da = None
self.dflip = None
self.classes = None
eulers = get_eulers_from(self.average_file)
#s = Symmetries.get("d7")
#eulers = s.gen_orientations("rand",{"n":EMUtil.get_image_count(self.average_file)})
self.specify_eulers(eulers)
#from emimagemx import EMDataListCache
#a = EMData.read_images(self.average_file)
#a = [test_image() for i in range(EMUtil.get_image_count(self.average_file))]
#print len(a),len(eulers)
#b = [a[i].set_attr("xform.projection",eulers[i]) for i in range(len(eulers))]
#b = [a[i].set_attr("ptcl_repr",1) for i in range(len(eulers))]
self.set_emdata_list_as_data(EMLightWeightParticleCache.from_file(self.average_file),"ptcl_repr")
#self.set_emdata_list_as_data(EMDataListCache(self.average_file),"ptcl_repr")
# self.set_emdata_list_as_data(a,"ptcl_repr")
self.force_update = True
self.au_point_selected(self.class_idx,None)
# if we have the same number of Eulers we can update everything
# if self.previous_len == len(eulers) : self.events_handlers["inspect"].repeat_event()
# else:self.events_handlers["inspect"].reset()
self.previous_len = len(eulers)
if not self.init_lock:self.updateGL()
get_application().setOverrideCursor(Qt.ArrowCursor)
def __get_file_headers(self,filename):
headers = []
n = EMUtil.get_image_count(filename)
for i in range(n):
e = EMData()
e.read_image(filename,i,True)
headers.append(e)
return headers
def au_point_selected(self,i,event=None):
if self.readfrom:
return
if i == None:
if event != None and event.modifiers()&Qt.ShiftModifier:
if self.special_euler != None:
self.special_euler = None
if not self.init_lock:self.regen_dl()
return
# self.arc_anim_points = None
self.projection = None
if self.euler_data:
# db = db_open_dict(self.average_file)
# a = db.get(i)
# print a["nx"]
# print self.average_file,i
# self.average = EMData(self.average_file,i)
# self.average["nx"]
self.average = self.euler_data[i]#
self.projection = EMData(self.projection_file,self.average.get_attr("projection_image_idx"))
self.average.process_inplace("normalize.toimage",{"to":self.projection})
try:
self.class_idx = self.average.get_attr("projection_image_idx")
print("%d (%d)"%(self.class_idx,self.average["ptcl_repr"]))
except:
self.class_idx = -1
else: return
#if self.projection == None and self.average == None: return
first = False
if self.proj_class_viewer == None:
first = True
self.proj_class_viewer = EMImageMXWidget(data=None,application=get_application())
# self.proj_class_viewer = EMImage2DWidget(image=None,application=get_application())
QtCore.QObject.connect(self.proj_class_viewer,QtCore.SIGNAL("module_closed"),self.on_mx_view_closed)
# self.proj_class_viewer.set_mouse_mode("App" )
QtCore.QObject.connect(self.proj_class_viewer,QtCore.SIGNAL("mx_image_selected"), self.mx_image_selected)
get_application().show_specific(self.proj_class_viewer)
self.proj_class_single = EMImage2DWidget(image=None,application=get_application())
QtCore.QObject.connect(self.proj_class_single,QtCore.SIGNAL("module_closed"),self.on_mx_view_closed)
# QtCore.QObject.connect(self.proj_class_single,QtCore.SIGNAL("mx_image_selected"), self.mx_image_selected)
get_application().show_specific(self.proj_class_single)
disp = []
if self.projection != None: disp.append(self.projection)
if self.average != None and self.projection!=None:
# ok, this really should be put into its own processor
#dataf = self.projection.do_fft()
#apix=self.projection["apix_x"]
#curve = dataf.calc_radial_dist(dataf["ny"], 0, 0.5,True)
#curve=[i/(dataf["nx"]*dataf["ny"])**2 for i in curve]
#xcurve=[i/(apix*2.0*dataf["ny"]) for i in range(len(curve))]
#xyd=XYData()
#xyd.set_xy_list(xcurve,curve)
#filt=self.average.process("filter.setstrucfac",{"apix":apix,"strucfac":xyd})
#filt.process_inplace("normalize.toimage",{"to":self.average})
self.projection["apix_x"]=self.average["apix_x"]
self.projection["apix_y"]=self.average["apix_y"]
self.projection["apix_z"]=self.average["apix_z"]
filt=self.projection.process("threshold.notzero")
filt.mult(self.average)
filt.process_inplace("filter.matchto",{"to":self.projection})
disp.append(filt)
if self.average!=None:
disp.append(self.average)
self.proj_class_viewer.set_data(disp)
self.proj_class_single.set_data(disp)
self.proj_class_viewer.updateGL()
self.proj_class_single.updateGL()
if self.particle_viewer != None:
self.mx_image_selected(None,None)
if first: self.proj_class_viewer.optimally_resize()
if i != self.special_euler:
self.special_euler = i
self.force_update = True
if not self.init_lock: self.updateGL()
def on_mx_view_closed(self):
self.proj_class_viewer = None
self.proj_class_single = None
def on_particle_mx_view_closed(self):
self.particle_viewer = None
def animation_done_event(self,animation):
pass
def alignment_time_animation(self,transforms):
if len(transforms) < 2: return
animation = OrientationListAnimation(self,transforms,self.radius)
self.register_animatable(animation)
def particle_selected(self,event,lc):
if lc != None:
d = lc[3]
ptcl_idx = d["Img #"]
data = self.au_data[self.refine_dir]
prj = []
cls_result = []
for l in data:
for s in l:
stag = base_name(s)
if len(stag) > 11 and stag[:11] == "projections":
prj.append(s)
elif len(stag) > 10 and stag[:10] == "cls_result":
cls_result.append(s)
transforms = []
if len(prj) != len(cls_result): RunTimeError("The number of cls_result files does not match the number of projection files?")
e = EMData()
for i,cr in enumerate(cls_result):
r = Region(0,ptcl_idx,1,1)
e.read_image(cr,0,False,r)
p = int(e.get(0))
e.read_image(prj[i],p,True)
transforms.append(e["xform.projection"])
self.alignment_time_animation(transforms)
def mx_image_selected(self,event,lc):
# self.arc_anim_points = None
get_application().setOverrideCursor(Qt.BusyCursor)
if lc != None: self.sel = lc[0]
if self.average != None:
included = []
if self.average.has_attr("class_ptcl_idxs"):
included = self.average["class_ptcl_idxs"]
excluded = []
if self.average.has_attr("exc_class_ptcl_idxs"):
excluded = self.average["exc_class_ptcl_idxs"]
all = included + excluded
#all.sort()
bdata = []
data = []
idx_included = []
running_idx = 0
from emimagemx import ApplyAttribute
for val in included:
bdata.append([self.particle_file,val,[ApplyAttribute("Img #",val)]])
idx_included.append(running_idx)
running_idx += 1
idx_excluded = []
for val in excluded:
bdata.append([self.particle_file,val,[ApplyAttribute("Img #",val)]])
idx_excluded.append(running_idx)
running_idx += 1
data = EMLightWeightParticleCache(bdata)
first = False
if self.particle_viewer == None:
first = True
self.particle_viewer = EMImageMXWidget(data=None,application=get_application())
self.particle_viewer.set_mouse_mode("App" )
QtCore.QObject.connect(self.particle_viewer,QtCore.SIGNAL("module_closed"),self.on_particle_mx_view_closed)
QtCore.QObject.connect(self.particle_viewer,QtCore.SIGNAL("mx_image_selected"), self.particle_selected)
get_application().show_specific(self.particle_viewer)
self.check_images_in_memory()
if self.sel== 0 or self.alignment_file == None:
self.particle_viewer.set_data(data)
else:
for i,[name,idx,f] in enumerate(bdata):
index = -1
if self.classes.get_xsize() == 1:
index = 0 # just assume it's the first one - this is potentially fatal assumption, but in obscure situations only
else:
for j in range(self.classes.get_xsize()):
if int(self.classes.get(j,idx)) == self.class_idx:
index = j
break
if index == -1:
print("couldn't find")
get_application().setOverrideCursor(Qt.ArrowCursor)
return
x = self.dx.get(index,idx)
y = self.dy.get(index,idx)
a = self.da.get(index,idx)
m = self.dflip.get(index,idx)
t = Transform({"type":"2d","alpha":a,"mirror":int(m)})
t.set_trans(x,y)
from emimagemx import ApplyTransform
f.append(ApplyTransform(t))
#data[i].transform(t)
self.particle_viewer.set_data(data)
if first:
self.particle_viewer.updateGL()
self.particle_viewer.optimally_resize()
self.particle_viewer.clear_sets(False)
self.particle_viewer.enable_set("Excluded",idx_excluded,True,False)
self.particle_viewer.enable_set("Included",idx_included,False,False)
self.particle_viewer.updateGL()
get_application().setOverrideCursor(Qt.ArrowCursor)
self.updateGL()
def check_images_in_memory(self):
if self.alignment_file != None:
if self.dx == None:
self.dx = EMData(self.alignment_file,2)
if self.dy == None:
self.dy = EMData(self.alignment_file,3)
if self.da == None:
self.da = EMData(self.alignment_file,4)
if self.dflip == None:
self.dflip = EMData(self.alignment_file,5)
if self.classes == None:
self.classes = EMData(self.alignment_file,0)
if self.inclusions == None:
self.inclusions = EMData(self.alignment_file,1)
def set_events_mode(self,mode):
if not mode in self.events_mode_list:
print("error, unknown events mode", mode)
return
else:
self.events_mode = mode
def closeEvent(self,event):
if self.inspector !=None: self.inspector.close()
if self.proj_class_viewer !=None: self.proj_class_viewer.close()
if self.proj_class_single !=None: self.proj_class_single.close()
if self.particle_viewer != None: self.particle_viewer.close()
get_application().close_specific(self)
self.emit(QtCore.SIGNAL("module_closed")) # this signal is
def __new__(cls,filename,application,force_plot=False,force_2d=False,old=None):
file_type = Util.get_filename_ext(filename)
em_file_type = EMUtil.get_image_ext_type(file_type)
if not file_exists(filename): return None
if force_plot and force_2d:
# ok this sucks but it suffices for the time being
print "Error, the force_plot and force_2d options are mutually exclusive"
return None
if force_plot:
from emplot2d import EMPlot2DWidget
if isinstance(old,EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
if em_file_type != IMAGE_UNKNOWN or filename[:4] == "bdb:":
n = EMUtil.get_image_count(filename)
nx,ny,nz = gimme_image_dimensions3D(filename)
if n > 1 and nz == 1:
if force_2d:
a = EMData()
data=a.read_images(filename)
else:
data = None # This is like a flag - the ImageMXWidget only needs the file name
elif nz == 1:
data = [EMData(filename,0)]
else:
data = EMData()
data.read_image(filename,0,not force_2d) # This should be 3-D. We read the header-only here
data = [data]
if data != None and len(data) == 1: data = data[0]
if force_2d or isinstance(data,EMData) and data.get_zsize()==1:
if isinstance(data,list) or data.get_ysize() != 1:
from emimage2d import EMImage2DWidget
if isinstance(old,EMImage2DWidget): widget = old
else: widget= EMImage2DWidget(application=application)
else:
from emplot2d import EMPlot2DWidget
if isinstance(old,EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
elif isinstance(data,EMData):
if isinstance(old,EMScene3D): widget = old
else: widget = EMScene3D()
# print n,data
for ii in xrange(n):
data=EMData(filename,ii)
datai = EMDataItem3D(data, transform=Transform())
widget.insertNewNode(os.path.basename(filename), datai, parentnode=widget)
isosurface = EMIsosurface(datai, transform=Transform())
widget.insertNewNode("Iso", isosurface, parentnode=datai)
return widget
elif data == None or isinstance(data,list):
from emimagemx import EMImageMXWidget
if isinstance(old,EMImageMXWidget): widget = old
else: widget = EMImageMXWidget(application=application)
data = filename
else:
print filename
raise # weirdness, this should never happen
widget.set_data(data,filename)
return widget
else:
from emplot2d import EMPlot2DWidget
if isinstance(old,EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
class ParticlesWindow:
def __init__(self, rctwidget):
self.rctwidget = rctwidget
self.window=EMImageMXWidget(application=self.rctwidget.parent_window)
self.window.set_display_values(["tilt","PImg#"])
self.window.set_mouse_mode("App")
self.window.setWindowTitle("Particles")
self.window.optimally_resize()
self.connect_signals()
self.listsofparts = []
self.numlists = 0
self.closed = False
def addlist(self, name):
data = []
data.append(name)
data.append(0)
data.append([])
self.listsofparts.append(data)
self.numlists = len(self.listsofparts)
def update_particles(self, particles, idx):
#print self.listsofparts[idx][0]
# reset the relevent list of particles
self.listsofparts[idx][1] = len(particles)
self.listsofparts[idx][2] = particles
# get the number of lists and the minimum number of particles in a given list..
listlength = 1e308
for lst in self.listsofparts:
listlength = min(listlength, lst[1])
i = 0
self.totparts = []
for part in xrange(listlength):
for lst in xrange(self.numlists):
self.listsofparts[lst][2][part].set_attr("tilt", self.listsofparts[lst][0])
self.listsofparts[lst][2][part].set_attr("PImg#", part)
self.totparts.append(self.listsofparts[lst][2][part])
i += 1
if self.totparts != []:
self.window.set_data(self.totparts)
self.window.updateGL()
def connect_signals(self):
QtCore.QObject.connect(self.window,QtCore.SIGNAL("mx_image_selected"),self.box_selected)
QtCore.QObject.connect(self.window,QtCore.SIGNAL("mx_mousedrag"),self.box_moved)
QtCore.QObject.connect(self.window,QtCore.SIGNAL("mx_mouseup"),self.box_released)
QtCore.QObject.connect(self.window,QtCore.SIGNAL("mx_boxdeleted"),self.box_image_deleted)
QtCore.QObject.connect(self.window,QtCore.SIGNAL("module_closed"),self.module_closed)
def box_selected(self,event,lc):
if lc == None or lc[0] == None: return
self.moving_box_data = [event.x(),event.y(),lc[0]]
def box_moved(self,event,scale):
winidx = self.moving_box_data[2] % self.numlists
ppidx = int(self.moving_box_data[2]/self.numlists)
if self.moving_box_data:
dx = 0.2*(event.x() - self.moving_box_data[0])
dy = 0.2*(self.moving_box_data[1] - event.y())
self.rctwidget.windowlist[winidx].boxes.move_box(ppidx,dx,dy)
self.rctwidget.windowlist[winidx].update_mainwin()
self.rctwidget.windowlist[winidx].update_particles()
def box_released(self, event,lc):
pass
def box_image_deleted(self,event,lc):
if lc == None or lc[0] == None: return
#delete all particle pairs
ppidx = int(lc[0]/self.numlists)
for i,window in enumerate(self.rctwidget.windowlist):
window.boxes.remove_box(ppidx,self.rctwidget.boxsize)
window.update_mainwin()
window.update_particles()
def module_closed(self):
E2saveappwin("e2rctboxer","particles",self.window.qt_parent)
pass
def __new__(cls,
data=None,
old=None,
app=None,
force_2d=False,
force_plot=False,
filename="",
replace=True):
"""This will create a new EMImage* object depending on the type of 'data'. If
old= is provided, and of the appropriate type, it will be used rather than creating
a new instance.
"""
if isinstance(data, EMData) and data.get_size() == 0:
raise RuntimeError(
"Can not display an EMData object that has no pixels")
from EMAN2 import remove_directories_from_name
if force_plot and force_2d:
# ok this sucks but it suffices for the time being
print(
"Error, the force_plot and force_2d options are mutually exclusive"
)
return None
if force_plot or (isinstance(data, EMData) and data.get_zsize() == 1
and data.get_ysize() == 1):
from emplot2d import EMPlot2DWidget
if old:
if isinstance(old, EMPlot2DWidget):
old.set_data(data, remove_directories_from_name(filename),
replace)
return old
widget = EMPlot2DWidget(application=app)
widget.set_data(data, remove_directories_from_name(filename),
replace)
return widget
elif force_2d or (isinstance(data, EMData) and data.get_zsize() == 1):
from emimage2d import EMImage2DWidget
if old:
if isinstance(old, EMImage2DWidget):
old.set_data(data, filename)
return old
widget = EMImage2DWidget(application=app)
widget.set_data(data, filename)
return widget
elif isinstance(data, EMData):
if isinstance(old, EMScene3D): widget = old
else: widget = EMScene3D()
data = EMDataItem3D(data, transform=Transform())
#data.setSelectedItem(True)
isosurface = EMIsosurface(data, transform=Transform())
widget.insertNewNode(os.path.basename(filename),
data,
parentnode=widget)
widget.insertNewNode("Iso", isosurface, parentnode=data)
return widget
elif isinstance(data, list) and isinstance(data[0], EMData):
from emimagemx import EMImageMXWidget
if old:
if isinstance(old, EMImageMXWidget):
old.set_data(data, filename)
return old
widget = EMImageMXWidget(application=app)
widget.set_data(data, filename)
return widget
elif isinstance(data, list):
from emplot3d import EMPlot3DWidgetNew
if (isinstance(data[0], list)
or isinstance(data[0], tuple)) and len(data) > 2:
if old:
if isinstance(old, EMPlot3DWidgetNew):
old.set_data(data,
remove_directories_from_name(filename),
replace)
return old
widget = EMPlot3DWidgetNew()
widget.set_data(data, remove_directories_from_name(filename),
replace)
return widget
else:
from emplot2d import EMPlot2DWidget
if old:
if isinstance(old, EMPlot2DWidget):
old.set_data(data,
remove_directories_from_name(filename),
replace)
return old
widget = EMPlot2DWidget(application=app)
widget.set_data(data, remove_directories_from_name(filename),
replace)
return widget
else:
raise Exception(
"data must be a single EMData object or a list of EMData objects"
)
def __new__(cls,
filename,
application,
force_plot=False,
force_2d=False,
old=None):
file_type = Util.get_filename_ext(filename)
em_file_type = EMUtil.get_image_ext_type(file_type)
if not file_exists(filename): return None
if force_plot and force_2d:
# ok this sucks but it suffices for the time being
print(
"Error, the force_plot and force_2d options are mutually exclusive"
)
return None
if force_plot:
from emplot2d import EMPlot2DWidget
if isinstance(old, EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
if em_file_type != IMAGE_UNKNOWN or filename[:4] == "bdb:":
n = EMUtil.get_image_count(filename)
nx, ny, nz = gimme_image_dimensions3D(filename)
if n > 1 and nz == 1:
if force_2d:
a = EMData()
data = a.read_images(filename)
else:
data = None # This is like a flag - the ImageMXWidget only needs the file name
elif nz == 1:
data = [EMData(filename, 0)]
else:
data = EMData()
data.read_image(
filename, 0, not force_2d
) # This should be 3-D. We read the header-only here
data = [data]
if data != None and len(data) == 1: data = data[0]
if force_2d or isinstance(data, EMData) and data.get_zsize() == 1:
if isinstance(data, list) or data.get_ysize() != 1:
from emimage2d import EMImage2DWidget
if isinstance(old, EMImage2DWidget): widget = old
else: widget = EMImage2DWidget(application=application)
else:
from emplot2d import EMPlot2DWidget
if isinstance(old, EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
elif isinstance(data, EMData):
if isinstance(old, EMScene3D): widget = old
else: widget = EMScene3D()
# print n,data
for ii in xrange(n):
data = EMData(filename, ii)
datai = EMDataItem3D(data, transform=Transform())
widget.insertNewNode(os.path.basename(filename),
datai,
parentnode=widget)
isosurface = EMIsosurface(datai, transform=Transform())
widget.insertNewNode("Iso", isosurface, parentnode=datai)
return widget
elif data == None or isinstance(data, list):
from emimagemx import EMImageMXWidget
if isinstance(old, EMImageMXWidget): widget = old
else: widget = EMImageMXWidget(application=application)
data = filename
else:
print(filename)
raise # weirdness, this should never happen
widget.set_data(data, filename)
return widget
else:
from emplot2d import EMPlot2DWidget
if isinstance(old, EMPlot2DWidget): widget = old
else: widget = EMPlot2DWidget(application=application)
widget.set_data_from_file(filename)
return widget
class GUIBoxer(QtGui.QWidget):
# Dictionary of autopickers
# to add a new one, provide name:(Qt_setup_function,picker_execution_function)
# Qt_setup_function(self,empty_grid_layout)
# picker_execution_function(self,...
aboxmodes = [ ("by Ref","auto_ref",boxerByRef), ("Gauss","auto_gauss",boxerGauss) ]
boxcolors = { "selected":(0.9,0.9,0.9), "manual":(0,0,0), "refgood":(0,0.8,0), "refbad":(0.8,0,0), "unknown":[.4,.4,.1], "auto_ref":(.1,.1,.4), "auto_gauss":(.4,.1,.4) }
def __init__(self,imagenames,voltage=None,apix=None,cs=None,ac=10.0,box=256,ptcl=200):
"""The 'new' e2boxer interface.
"""
QtGui.QWidget.__init__(self,None)
# self.setWindowIcon(QtGui.QIcon(get_image_directory() + "ctf.png"))
self.data=None
self.curfilename = None # current selected file for boxing
self.filenames=imagenames # list of available filenames
self.micrograph=None
self.boxes=None
self.defaultvoltage=voltage
self.defaultapix=apix
self.defaultcs=cs
self.defaultac=ac
self.db = None # open JSON file for current image
self.wimage=EMImage2DWidget()
self.wimage.setWindowTitle("Micrograph")
self.wparticles=EMImageMXWidget()
self.wparticles.setWindowTitle("Particles")
self.wrefs=EMImageMXWidget()
self.wrefs.setWindowTitle("Box Refs")
self.wbadrefs=EMImageMXWidget()
self.wbadrefs.setWindowTitle("Bad Box Refs")
#self.wfft=EMImage2DWidget()
#self.wfft.setWindowTitle("e2evalimage - 2D FFT")
#self.wplot=EMPlot2DWidget()
#self.wplot.setWindowTitle("e2evalimage - Plot")
self.wimage.connect(self.wimage,QtCore.SIGNAL("mousedown"),self.imgmousedown)
self.wimage.connect(self.wimage,QtCore.SIGNAL("mousedrag"),self.imgmousedrag)
self.wimage.connect(self.wimage,QtCore.SIGNAL("mouseup") ,self.imgmouseup)
self.wparticles.connect(self.wparticles,QtCore.SIGNAL("mousedown"),self.ptclmousedown)
self.wparticles.connect(self.wparticles,QtCore.SIGNAL("mousedrag"),self.ptclmousedrag)
self.wparticles.connect(self.wparticles,QtCore.SIGNAL("mouseup") ,self.ptclmouseup)
self.wrefs.connect(self.wparticles,QtCore.SIGNAL("mousedown"),self.refmousedown)
self.wrefs.connect(self.wparticles,QtCore.SIGNAL("mousedrag"),self.refmousedrag)
self.wrefs.connect(self.wparticles,QtCore.SIGNAL("mouseup") ,self.refmouseup)
self.wimage.mmode="app"
self.wparticles.mmode="app"
# This object is itself a widget we need to set up
self.gbl = QtGui.QGridLayout(self)
self.gbl.setMargin(8)
self.gbl.setSpacing(6)
# Micrograph list
self.setlist=QtGui.QListWidget(self)
self.setlist.setSizePolicy(QtGui.QSizePolicy.Preferred,QtGui.QSizePolicy.Expanding)
for i in imagenames:
self.setlist.addItem(i)
self.gbl.addWidget(self.setlist,0,0,12,2)
self.setlist.connect(self.setlist,QtCore.SIGNAL("currentRowChanged(int)"),self.newSet)
self.setlist.connect(self.setlist,QtCore.SIGNAL("keypress"),self.listKey)
# Mouse Modes
self.mmode="manual"
self.boxmm=QtGui.QGroupBox("Mouse Mode",self)
self.boxmm.setFlat(False)
self.gbl.addWidget(self.boxmm,0,2,3,2)
self.hbl0=QtGui.QHBoxLayout(self.boxmm)
self.bmmanual=QtGui.QPushButton("Manual")
self.bmmanual.setToolTip("Manual selection of particles. No impact on autoselection.")
self.bmmanual.setAutoExclusive(True)
self.bmmanual.setCheckable(True)
self.bmmanual.setChecked(True)
self.hbl0.addWidget(self.bmmanual)
self.bmdel=QtGui.QPushButton("Delete")
self.bmdel.setToolTip("Delete particles from any mode. Can also shift-click in other mouse modes.")
self.bmdel.setAutoExclusive(True)
self.bmdel.setCheckable(True)
self.hbl0.addWidget(self.bmdel)
self.bmgref=QtGui.QPushButton("Good Refs")
self.bmgref.setToolTip("Identify some good particles. Available to all autoboxers.")
self.bmgref.setAutoExclusive(True)
self.bmgref.setCheckable(True)
self.hbl0.addWidget(self.bmgref)
self.bmbref=QtGui.QPushButton("Bad Refs")
self.bmbref.setToolTip("Identify regions which should not be selected as particles.")
self.bmbref.setAutoExclusive(True)
self.bmbref.setCheckable(True)
self.hbl0.addWidget(self.bmbref)
QtCore.QObject.connect(self.bmmanual,QtCore.SIGNAL("clicked(bool)"),self.setMouseManual)
QtCore.QObject.connect(self.bmdel,QtCore.SIGNAL("clicked(bool)"),self.setMouseDel)
QtCore.QObject.connect(self.bmgref,QtCore.SIGNAL("clicked(bool)"),self.setMouseGoodRef)
QtCore.QObject.connect(self.bmbref,QtCore.SIGNAL("clicked(bool)"),self.setMouseBadRef)
# Global parameters
self.boxparm=QtGui.QGroupBox("Parameters",self)
self.boxparm.setFlat(False)
self.gbl.addWidget(self.boxparm,3,2,3,3)
self.gbl1=QtGui.QGridLayout(self.boxparm)
self.gbl1.setMargin(8)
self.gbl1.setSpacing(6)
self.vbbsize = ValBox(label="Box Size:",value=box)
self.gbl1.addWidget(self.vbbsize,0,0)
self.vbbpsize = ValBox(label="Ptcl Size:",value=ptcl)
self.gbl1.addWidget(self.vbbpsize,0,1)
self.vbbapix = ValBox(label="A/pix:",value=apix)
self.gbl1.addWidget(self.vbbapix,0,2)
self.vbvoltage = ValBox(label="Voltage:",value=voltage)
self.gbl1.addWidget(self.vbvoltage,1,0)
self.vbbac = ValBox(label="% AC:",value=ac)
self.gbl1.addWidget(self.vbbac,1,1)
self.vbcs = ValBox(label="Cs:",value=cs)
self.gbl1.addWidget(self.vbcs,1,2)
# Autoboxing Tabs
self.autolbl = QtGui.QLabel("Autoboxing Methods:")
self.gbl.addWidget(self.autolbl,7,2)
self.autotab = QtGui.QTabWidget()
self.gbl.addWidget(self.autotab,8,2,6,3)
self.bautobox = QtGui.QPushButton("Autobox")
self.gbl.addWidget(self.bautobox,7,4)
QtCore.QObject.connect(self.bautobox,QtCore.SIGNAL("clicked(bool)"),self.doAutoBox)
# Individual tabs from Dictionary
self.abwid=[]
for name,bname,cls in GUIBoxer.aboxmodes:
w=QtGui.QWidget()
gl=QtGui.QGridLayout(w)
self.abwid.append((w,gl))
cls.setup_gui(gl)
self.autotab.addTab(w,name)
self.setWindowTitle("e2boxer21 - Control Panel")
self.wimage.show()
# self.wfft.show()
# self.wplot.show()
E2loadappwin("e2boxer21","main",self)
E2loadappwin("e2boxer21","image",self.wimage.qt_parent)
E2loadappwin("e2boxer21","particles",self.wparticles.qt_parent)
E2loadappwin("e2boxer21","refs",self.wrefs.qt_parent)
E2loadappwin("e2boxer21","badrefs",self.wbadrefs.qt_parent)
self.newSet(0)
#QWidget *firstPageWidget = new QWidget;
#QWidget *secondPageWidget = new QWidget;
#QWidget *thirdPageWidget = new QWidget;
#QStackedWidget *stackedWidget = new QStackedWidget;
#stackedWidget->addWidget(firstPageWidget);
#stackedWidget->addWidget(secondPageWidget);
#stackedWidget->addWidget(thirdPageWidget);
#QVBoxLayout *layout = new QVBoxLayout;
#layout->addWidget(stackedWidget);
#setLayout(layout);
#QComboBox *pageComboBox = new QComboBox;
#pageComboBox->addItem(tr("Page 1"));
#pageComboBox->addItem(tr("Page 2"));
#pageComboBox->addItem(tr("Page 3"));
#connect(pageComboBox, SIGNAL(activated(int)),stackedWidget, SLOT(setCurrentIndex(int)));
#self.lboxmode=QtGui.QLabel("Mode:",self)
#self.gbl.addWidget(self.lboxmode,10,0)
#self.sboxmode=QtGui.QComboBox(self)
#self.sboxmode.addItem("Manual")
#self.sboxmode.addItem("Reference")
#self.sboxmode.setCurrentIndex(1)
#self.gbl.addWidget(self.sboxmode,10,1)
#self.lanmode=QtGui.QLabel("Annotate:",self)
#self.gbl.addWidget(self.lanmode,12,0)
#self.sanmode=QtGui.QComboBox(self)
#self.sanmode.addItem("Box")
#self.sanmode.addItem("Box+dot")
#self.sanmode.addItem("Circle")
#self.sanmode.addItem("None")
#self.gbl.addWidget(self.sanmode,12,1)
#self.sdefocus=ValSlider(self,(0,5),"Defocus:",0.0,90)
#self.gbl.addWidget(self.sdefocus,0,2,1,3)
#self.squality=ValSlider(self,(0,9),"Quality (0-9):",0,90)
#self.squality.setIntonly(True)
#self.gbl.addWidget(self.squality,6,2,1,3)
#self.brefit=QtGui.QPushButton("Autobox")
#self.gbl.addWidget(self.brefit,7,2)
#self.bclrauto=QtGui.QPushButton("Clear Auto")
#self.gbl.addWidget(self.bclrauto,7,3)
#self.bclrall=QtGui.QPushButton("Clear All")
#self.gbl.addWidget(self.bclrall,7,4)
#self.sapix=ValBox(self,(0,500),"A/pix:",1.0,90)
#if self.defaultapix!=None : self.sapix.setValue(self.defaultapix)
#self.gbl.addWidget(self.sapix,10,2)
#self.svoltage=ValBox(self,(0,500),"Voltage (kV):",200,90)
#if self.defaultvoltage!=None : self.svoltage.setValue(self.defaultvoltage)
#self.gbl.addWidget(self.svoltage,11,2)
#self.scs=ValBox(self,(0,5),"Cs (mm):",4.1,90)
#if self.defaultcs!=None : self.scs.setValue(self.defaultcs)
#self.gbl.addWidget(self.scs,12,2)
#self.sboxsize=ValBox(self,(0,500),"Box Size:",256,90)
#self.sboxsize.intonly=True
#self.gbl.addWidget(self.sboxsize,13,2)
#self.sptclsize=ValBox(self,(0,500),"Ptcl Size:",256,90)
#self.sptclsize.intonly=True
#self.gbl.addWidget(self.sptclsize,14,2)
#QtCore.QObject.connect(self.sdefocus, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.sapix, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.svoltage, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.scs, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.sboxsize, QtCore.SIGNAL("valueChanged"), self.newBox)
## QtCore.QObject.connect(self.soversamp, QtCore.SIGNAL("valueChanged"), self.newBox)
#QtCore.QObject.connect(self.squality,QtCore.SIGNAL("valueChanged"),self.newQualityFactor)
#QtCore.QObject.connect(self.setlist,QtCore.SIGNAL("currentRowChanged(int)"),self.newSet)
#QtCore.QObject.connect(self.setlist,QtCore.SIGNAL("keypress"),self.listkey)
#QtCore.QObject.connect(self.sboxmode,QtCore.SIGNAL("currentIndexChanged(int)"),self.newBoxMode)
#self.resize(720,380) # figured these values out by printing the width and height in resize event
#### This section is responsible for background updates
#self.busy=False
#self.needupdate=True
#self.needredisp=False
#self.procthread=None
#self.errors=None # used to communicate errors back from the reprocessing thread
#self.timer=QTimer()
#QtCore.QObject.connect(self.timer, QtCore.SIGNAL("timeout()"), self.timeOut)
#self.timer.start(100)
# self.recalc()
def setMouseManual(self,x):
self.mmode="manual"
def setMouseDel(self,x):
self.mmode="del"
def setMouseGoodRef(self,x):
self.mmode="refgood"
def setMouseBadRef(self,x):
self.mmode="refbad"
def imgmousedown(self,event) :
m=self.wimage.scr_to_img((event.x(),event.y()))
boxsize2=self.vbbsize.getValue()//2
ptclsize=self.vbbpsize.getValue()
self.curbox=0
# check to see if click was inside an existing box, in which case we move it
for i in self.boxes:
if abs(m.x-i[0])<boxsize2 and abs(m.y-i[1])<boxsize2 :
self.curbox=i
break
else :
# Create a new box
if self.mmode=="del" : return # This is for creating a new box, so clearly not desirable in delete mode
self.curbox=len(self.boxes)
self.boxes.append((m[0],m[1],self.mmode))
self.__addBox(self.curbox,self.boxes[-1])
#self.guiim.add_shape("cen",["rect",.9,.9,.4,x0,y0,x0+2,y0+2,1.0])
def imgmousedrag(self,event) :
if self.calcmode==0:
m=self.wimage.scr_to_img((event.x(),event.y()))
parms=self.parms[self.curset]
parms[2]=(m[0]-parms[0]/2,m[1]-parms[0]/2)
self.needredisp=True
self.recalc()
# box deletion when shift held down
#if event.modifiers()&Qt.ShiftModifier:
#for i,j in enumerate(self.boxes):
def imgmouseup(self,event) :
m=self.wimage.scr_to_img((event.x(),event.y()))
if self.calcmode==1:
parms=self.parms[self.curset]
nx=self.data["nx"]/parms[0]-1
grid=int((m[0]-parms[0]/2)/parms[0])+int((m[1]-parms[0]/2)/parms[0])*nx
if grid in parms[3] : parms[3].remove(grid)
else: parms[3].add(grid)
self.needredisp=True
self.recalc()
def ptclmousedown(self,event) :
return
def ptclmousedrag(self,event) :
return
def ptclmouseup(self,event) :
return
def refmousedown(self,event) :
return
def refmousedrag(self,event) :
return
def refmouseup(self,event) :
return
def newSet(self,val):
"called when a new data set is selected from the list"
first=True
newfilename=str(self.setlist.item(val).text())
if newfilename==self.curfilename : return
# Write the current image parameters to the database
if self.curfilename!=None and self.boxes!=None :
self.save_boxes()
first=False
self.micrograph=load_micrograph(newfilename)
self.wimage.set_data(self.micrograph)
if first : E2loadappwin("e2boxer21","image",self.wimage.qt_parent)
self.curfilename=newfilename
self.restore_boxes()
def save_boxes(self):
js=js_open_dict(info_name(self.curfilename))
js["boxes"]=self.boxes
def restore_boxes(self):
# first we restore the list of box locations
js=js_open_dict(info_name(self.curfilename))
try: self.boxes=js["boxes"]
except: self.boxes=[]
boxsize=self.vbbsize.getValue()
ptclsize=self.vbbpsize.getValue()
micro=self.wimage.get_data()
self.wimage.del_shapes()
if len(self.boxes)==0 :
self.wparticles.set_data([])
return
# Then we extract the actual boxed out particles
goodrefs=[]
badrefs=[]
self.particles=[]
for i,box in enumerate(self.boxes):
self.__addBox(i,box)
# extract the data
boxim=self.micrograph.get_clip(Region(box[0]-boxsize//2,box[1]-boxsize//2,boxsize,boxsize))
boxim["ptcl_source_coord"]=(box[0],box[1])
if box[2]=="refgood":
goodrefs.append(boxim)
elif box[2]=="refbad":
badrefs.append(boxim)
else:
self.particles.append(boxim)
# finally redisplay as appropriate
self.wimage.update()
self.wparticles.set_data(self.particles)
if len(self.particles)>0 : self.wparticles.show()
if len(goodrefs)+len(badrefs)!=0:
self.goodrefs=goodrefs
self.badrefs=badrefs
self.wrefs.set_data(self.goodrefs)
self.wbadrefs.set_data(self.badrefs)
if len(self.goodrefs)>0 : self.wrefs.show()
if len(self.badrefs)>0 : self.wbadrefs.show()
def __addBox(self,i,box):
"""takes the number of the box in self.boxes and the (x,y,mode) tuple and displays it"""
# Display the actual box
boxsize=self.vbbsize.getValue()
ptclsize=self.vbbpsize.getValue()
try: color=self.boxcolors[box[2]]
except: color=self.boxcolors["unknown"]
self.wimage.add_shape("box{}".format(i),EMShape(("rect",color[0],color[1],color[2],box[0]-boxsize//2,box[1]-boxsize//2,box[0]+boxsize//2,box[1]+boxsize//2,2)))
self.wimage.add_shape("cir{}".format(i),EMShape(("circle",color[0],color[1],color[2],box[0],box[1],ptclsize/2,1.5)))
def listKey(self,event):
pass
#if event.key()>=Qt.Key_0 and event.key()<=Qt.Key_9 :
#q=int(event.key())-Qt.Key_0
#self.squality.setValue(q)
#elif event.key() == Qt.Key_Left:
#self.sdefocus.setValue(self.sdefocus.getValue()-0.03)
#elif event.key() == Qt.Key_Right:
#self.sdefocus.setValue(self.sdefocus.getValue()+0.03)
#elif event.key()==Qt.Key_I :
#self.doImport()
#elif event.key()==Qt.Key_U :
#self.unImport()
def doAutoBox(self,b):
"""Autobox button pressed, find the right algorithm and call it"""
name,bname,fn1=self.aboxmodes[self.autotab.currentIndex()]
print name," called"
def closeEvent(self,event):
# QtGui.QWidget.closeEvent(self,event)
E2saveappwin("e2boxer21","main",self)
E2saveappwin("e2boxer21","image",self.wimage.qt_parent)
E2saveappwin("e2boxer21","particles",self.wparticles.qt_parent)
E2saveappwin("e2boxer21","refs",self.wrefs.qt_parent)
E2saveappwin("e2boxer21","badrefs",self.wbadrefs.qt_parent)
#self.writeCurParm()
event.accept()
QtGui.qApp.exit(0)
class GUIBoxer(QtGui.QWidget):
# Dictionary of autopickers
# to add a new one, provide name:(Qt_setup_function,picker_execution_function)
# Qt_setup_function(self,empty_grid_layout)
# picker_execution_function(self,...
aboxmodes = [("by Ref", "auto_ref", boxerByRef),
("Gauss", "auto_gauss", boxerGauss)]
boxcolors = {
"selected": (0.9, 0.9, 0.9),
"manual": (0, 0, 0),
"refgood": (0, 0.8, 0),
"refbad": (0.8, 0, 0),
"unknown": [.4, .4, .1],
"auto_ref": (.1, .1, .4),
"auto_gauss": (.4, .1, .4)
}
def __init__(self,
imagenames,
voltage=None,
apix=None,
cs=None,
ac=10.0,
box=256,
ptcl=200):
"""The 'new' e2boxer interface.
"""
QtGui.QWidget.__init__(self, None)
# self.setWindowIcon(QtGui.QIcon(get_image_directory() + "ctf.png"))
self.data = None
self.curfilename = None # current selected file for boxing
self.filenames = imagenames # list of available filenames
self.micrograph = None
self.boxes = None
self.defaultvoltage = voltage
self.defaultapix = apix
self.defaultcs = cs
self.defaultac = ac
self.db = None # open JSON file for current image
self.wimage = EMImage2DWidget()
self.wimage.setWindowTitle("Micrograph")
self.wparticles = EMImageMXWidget()
self.wparticles.setWindowTitle("Particles")
self.wrefs = EMImageMXWidget()
self.wrefs.setWindowTitle("Box Refs")
self.wbadrefs = EMImageMXWidget()
self.wbadrefs.setWindowTitle("Bad Box Refs")
#self.wfft=EMImage2DWidget()
#self.wfft.setWindowTitle("e2evalimage - 2D FFT")
#self.wplot=EMPlot2DWidget()
#self.wplot.setWindowTitle("e2evalimage - Plot")
self.wimage.connect(self.wimage, QtCore.SIGNAL("mousedown"),
self.imgmousedown)
self.wimage.connect(self.wimage, QtCore.SIGNAL("mousedrag"),
self.imgmousedrag)
self.wimage.connect(self.wimage, QtCore.SIGNAL("mouseup"),
self.imgmouseup)
self.wparticles.connect(self.wparticles, QtCore.SIGNAL("mousedown"),
self.ptclmousedown)
self.wparticles.connect(self.wparticles, QtCore.SIGNAL("mousedrag"),
self.ptclmousedrag)
self.wparticles.connect(self.wparticles, QtCore.SIGNAL("mouseup"),
self.ptclmouseup)
self.wrefs.connect(self.wparticles, QtCore.SIGNAL("mousedown"),
self.refmousedown)
self.wrefs.connect(self.wparticles, QtCore.SIGNAL("mousedrag"),
self.refmousedrag)
self.wrefs.connect(self.wparticles, QtCore.SIGNAL("mouseup"),
self.refmouseup)
self.wimage.mmode = "app"
self.wparticles.mmode = "app"
# This object is itself a widget we need to set up
self.gbl = QtGui.QGridLayout(self)
self.gbl.setMargin(8)
self.gbl.setSpacing(6)
# Micrograph list
self.setlist = QtGui.QListWidget(self)
self.setlist.setSizePolicy(QtGui.QSizePolicy.Preferred,
QtGui.QSizePolicy.Expanding)
for i in imagenames:
self.setlist.addItem(i)
self.gbl.addWidget(self.setlist, 0, 0, 12, 2)
self.setlist.connect(self.setlist,
QtCore.SIGNAL("currentRowChanged(int)"),
self.newSet)
self.setlist.connect(self.setlist, QtCore.SIGNAL("keypress"),
self.listKey)
# Mouse Modes
self.mmode = "manual"
self.boxmm = QtGui.QGroupBox("Mouse Mode", self)
self.boxmm.setFlat(False)
self.gbl.addWidget(self.boxmm, 0, 2, 3, 2)
self.hbl0 = QtGui.QHBoxLayout(self.boxmm)
self.bmmanual = QtGui.QPushButton("Manual")
self.bmmanual.setToolTip(
"Manual selection of particles. No impact on autoselection.")
self.bmmanual.setAutoExclusive(True)
self.bmmanual.setCheckable(True)
self.bmmanual.setChecked(True)
self.hbl0.addWidget(self.bmmanual)
self.bmdel = QtGui.QPushButton("Delete")
self.bmdel.setToolTip(
"Delete particles from any mode. Can also shift-click in other mouse modes."
)
self.bmdel.setAutoExclusive(True)
self.bmdel.setCheckable(True)
self.hbl0.addWidget(self.bmdel)
self.bmgref = QtGui.QPushButton("Good Refs")
self.bmgref.setToolTip(
"Identify some good particles. Available to all autoboxers.")
self.bmgref.setAutoExclusive(True)
self.bmgref.setCheckable(True)
self.hbl0.addWidget(self.bmgref)
self.bmbref = QtGui.QPushButton("Bad Refs")
self.bmbref.setToolTip(
"Identify regions which should not be selected as particles.")
self.bmbref.setAutoExclusive(True)
self.bmbref.setCheckable(True)
self.hbl0.addWidget(self.bmbref)
QtCore.QObject.connect(self.bmmanual, QtCore.SIGNAL("clicked(bool)"),
self.setMouseManual)
QtCore.QObject.connect(self.bmdel, QtCore.SIGNAL("clicked(bool)"),
self.setMouseDel)
QtCore.QObject.connect(self.bmgref, QtCore.SIGNAL("clicked(bool)"),
self.setMouseGoodRef)
QtCore.QObject.connect(self.bmbref, QtCore.SIGNAL("clicked(bool)"),
self.setMouseBadRef)
# Global parameters
self.boxparm = QtGui.QGroupBox("Parameters", self)
self.boxparm.setFlat(False)
self.gbl.addWidget(self.boxparm, 3, 2, 3, 3)
self.gbl1 = QtGui.QGridLayout(self.boxparm)
self.gbl1.setMargin(8)
self.gbl1.setSpacing(6)
self.vbbsize = ValBox(label="Box Size:", value=box)
self.gbl1.addWidget(self.vbbsize, 0, 0)
self.vbbpsize = ValBox(label="Ptcl Size:", value=ptcl)
self.gbl1.addWidget(self.vbbpsize, 0, 1)
self.vbbapix = ValBox(label="A/pix:", value=apix)
self.gbl1.addWidget(self.vbbapix, 0, 2)
self.vbvoltage = ValBox(label="Voltage:", value=voltage)
self.gbl1.addWidget(self.vbvoltage, 1, 0)
self.vbbac = ValBox(label="% AC:", value=ac)
self.gbl1.addWidget(self.vbbac, 1, 1)
self.vbcs = ValBox(label="Cs:", value=cs)
self.gbl1.addWidget(self.vbcs, 1, 2)
# Autoboxing Tabs
self.autolbl = QtGui.QLabel("Autoboxing Methods:")
self.gbl.addWidget(self.autolbl, 7, 2)
self.autotab = QtGui.QTabWidget()
self.gbl.addWidget(self.autotab, 8, 2, 6, 3)
self.bautobox = QtGui.QPushButton("Autobox")
self.gbl.addWidget(self.bautobox, 7, 4)
QtCore.QObject.connect(self.bautobox, QtCore.SIGNAL("clicked(bool)"),
self.doAutoBox)
# Individual tabs from Dictionary
self.abwid = []
for name, bname, cls in GUIBoxer.aboxmodes:
w = QtGui.QWidget()
gl = QtGui.QGridLayout(w)
self.abwid.append((w, gl))
cls.setup_gui(gl)
self.autotab.addTab(w, name)
self.setWindowTitle("e2boxer21 - Control Panel")
self.wimage.show()
# self.wfft.show()
# self.wplot.show()
E2loadappwin("e2boxer21", "main", self)
E2loadappwin("e2boxer21", "image", self.wimage.qt_parent)
E2loadappwin("e2boxer21", "particles", self.wparticles.qt_parent)
E2loadappwin("e2boxer21", "refs", self.wrefs.qt_parent)
E2loadappwin("e2boxer21", "badrefs", self.wbadrefs.qt_parent)
self.newSet(0)
#QWidget *firstPageWidget = new QWidget;
#QWidget *secondPageWidget = new QWidget;
#QWidget *thirdPageWidget = new QWidget;
#QStackedWidget *stackedWidget = new QStackedWidget;
#stackedWidget->addWidget(firstPageWidget);
#stackedWidget->addWidget(secondPageWidget);
#stackedWidget->addWidget(thirdPageWidget);
#QVBoxLayout *layout = new QVBoxLayout;
#layout->addWidget(stackedWidget);
#setLayout(layout);
#QComboBox *pageComboBox = new QComboBox;
#pageComboBox->addItem(tr("Page 1"));
#pageComboBox->addItem(tr("Page 2"));
#pageComboBox->addItem(tr("Page 3"));
#connect(pageComboBox, SIGNAL(activated(int)),stackedWidget, SLOT(setCurrentIndex(int)));
#self.lboxmode=QtGui.QLabel("Mode:",self)
#self.gbl.addWidget(self.lboxmode,10,0)
#self.sboxmode=QtGui.QComboBox(self)
#self.sboxmode.addItem("Manual")
#self.sboxmode.addItem("Reference")
#self.sboxmode.setCurrentIndex(1)
#self.gbl.addWidget(self.sboxmode,10,1)
#self.lanmode=QtGui.QLabel("Annotate:",self)
#self.gbl.addWidget(self.lanmode,12,0)
#self.sanmode=QtGui.QComboBox(self)
#self.sanmode.addItem("Box")
#self.sanmode.addItem("Box+dot")
#self.sanmode.addItem("Circle")
#self.sanmode.addItem("None")
#self.gbl.addWidget(self.sanmode,12,1)
#self.sdefocus=ValSlider(self,(0,5),"Defocus:",0.0,90)
#self.gbl.addWidget(self.sdefocus,0,2,1,3)
#self.squality=ValSlider(self,(0,9),"Quality (0-9):",0,90)
#self.squality.setIntonly(True)
#self.gbl.addWidget(self.squality,6,2,1,3)
#self.brefit=QtGui.QPushButton("Autobox")
#self.gbl.addWidget(self.brefit,7,2)
#self.bclrauto=QtGui.QPushButton("Clear Auto")
#self.gbl.addWidget(self.bclrauto,7,3)
#self.bclrall=QtGui.QPushButton("Clear All")
#self.gbl.addWidget(self.bclrall,7,4)
#self.sapix=ValBox(self,(0,500),"A/pix:",1.0,90)
#if self.defaultapix!=None : self.sapix.setValue(self.defaultapix)
#self.gbl.addWidget(self.sapix,10,2)
#self.svoltage=ValBox(self,(0,500),"Voltage (kV):",200,90)
#if self.defaultvoltage!=None : self.svoltage.setValue(self.defaultvoltage)
#self.gbl.addWidget(self.svoltage,11,2)
#self.scs=ValBox(self,(0,5),"Cs (mm):",4.1,90)
#if self.defaultcs!=None : self.scs.setValue(self.defaultcs)
#self.gbl.addWidget(self.scs,12,2)
#self.sboxsize=ValBox(self,(0,500),"Box Size:",256,90)
#self.sboxsize.intonly=True
#self.gbl.addWidget(self.sboxsize,13,2)
#self.sptclsize=ValBox(self,(0,500),"Ptcl Size:",256,90)
#self.sptclsize.intonly=True
#self.gbl.addWidget(self.sptclsize,14,2)
#QtCore.QObject.connect(self.sdefocus, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.sapix, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.svoltage, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.scs, QtCore.SIGNAL("valueChanged"), self.newCTF)
#QtCore.QObject.connect(self.sboxsize, QtCore.SIGNAL("valueChanged"), self.newBox)
## QtCore.QObject.connect(self.soversamp, QtCore.SIGNAL("valueChanged"), self.newBox)
#QtCore.QObject.connect(self.squality,QtCore.SIGNAL("valueChanged"),self.newQualityFactor)
#QtCore.QObject.connect(self.setlist,QtCore.SIGNAL("currentRowChanged(int)"),self.newSet)
#QtCore.QObject.connect(self.setlist,QtCore.SIGNAL("keypress"),self.listkey)
#QtCore.QObject.connect(self.sboxmode,QtCore.SIGNAL("currentIndexChanged(int)"),self.newBoxMode)
#self.resize(720,380) # figured these values out by printing the width and height in resize event
#### This section is responsible for background updates
#self.busy=False
#self.needupdate=True
#self.needredisp=False
#self.procthread=None
#self.errors=None # used to communicate errors back from the reprocessing thread
#self.timer=QTimer()
#QtCore.QObject.connect(self.timer, QtCore.SIGNAL("timeout()"), self.timeOut)
#self.timer.start(100)
# self.recalc()
def setMouseManual(self, x):
self.mmode = "manual"
def setMouseDel(self, x):
self.mmode = "del"
def setMouseGoodRef(self, x):
self.mmode = "refgood"
def setMouseBadRef(self, x):
self.mmode = "refbad"
def imgmousedown(self, event):
m = self.wimage.scr_to_img((event.x(), event.y()))
boxsize2 = self.vbbsize.getValue() // 2
ptclsize = self.vbbpsize.getValue()
self.curbox = 0
# check to see if click was inside an existing box, in which case we move it
for i in self.boxes:
if abs(m.x - i[0]) < boxsize2 and abs(m.y - i[1]) < boxsize2:
self.curbox = i
break
else:
# Create a new box
if self.mmode == "del":
return # This is for creating a new box, so clearly not desirable in delete mode
self.curbox = len(self.boxes)
self.boxes.append((m[0], m[1], self.mmode))
self.__addBox(self.curbox, self.boxes[-1])
#self.guiim.add_shape("cen",["rect",.9,.9,.4,x0,y0,x0+2,y0+2,1.0])
def imgmousedrag(self, event):
if self.calcmode == 0:
m = self.wimage.scr_to_img((event.x(), event.y()))
parms = self.parms[self.curset]
parms[2] = (m[0] - parms[0] / 2, m[1] - parms[0] / 2)
self.needredisp = True
self.recalc()
# box deletion when shift held down
#if event.modifiers()&Qt.ShiftModifier:
#for i,j in enumerate(self.boxes):
def imgmouseup(self, event):
m = self.wimage.scr_to_img((event.x(), event.y()))
if self.calcmode == 1:
parms = self.parms[self.curset]
nx = self.data["nx"] / parms[0] - 1
grid = int((m[0] - parms[0] / 2) / parms[0]) + int(
(m[1] - parms[0] / 2) / parms[0]) * nx
if grid in parms[3]: parms[3].remove(grid)
else: parms[3].add(grid)
self.needredisp = True
self.recalc()
def ptclmousedown(self, event):
return
def ptclmousedrag(self, event):
return
def ptclmouseup(self, event):
return
def refmousedown(self, event):
return
def refmousedrag(self, event):
return
def refmouseup(self, event):
return
def newSet(self, val):
"called when a new data set is selected from the list"
first = True
newfilename = str(self.setlist.item(val).text())
if newfilename == self.curfilename: return
# Write the current image parameters to the database
if self.curfilename != None and self.boxes != None:
self.save_boxes()
first = False
self.micrograph = load_micrograph(newfilename)
self.wimage.set_data(self.micrograph)
if first: E2loadappwin("e2boxer21", "image", self.wimage.qt_parent)
self.curfilename = newfilename
self.restore_boxes()
def save_boxes(self):
js = js_open_dict(info_name(self.curfilename))
js["boxes"] = self.boxes
def restore_boxes(self):
# first we restore the list of box locations
js = js_open_dict(info_name(self.curfilename))
try:
self.boxes = js["boxes"]
except:
self.boxes = []
boxsize = self.vbbsize.getValue()
ptclsize = self.vbbpsize.getValue()
micro = self.wimage.get_data()
self.wimage.del_shapes()
if len(self.boxes) == 0:
self.wparticles.set_data([])
return
# Then we extract the actual boxed out particles
goodrefs = []
badrefs = []
self.particles = []
for i, box in enumerate(self.boxes):
self.__addBox(i, box)
# extract the data
boxim = self.micrograph.get_clip(
Region(box[0] - boxsize // 2, box[1] - boxsize // 2, boxsize,
boxsize))
boxim["ptcl_source_coord"] = (box[0], box[1])
if box[2] == "refgood":
goodrefs.append(boxim)
elif box[2] == "refbad":
badrefs.append(boxim)
else:
self.particles.append(boxim)
# finally redisplay as appropriate
self.wimage.update()
self.wparticles.set_data(self.particles)
if len(self.particles) > 0: self.wparticles.show()
if len(goodrefs) + len(badrefs) != 0:
self.goodrefs = goodrefs
self.badrefs = badrefs
self.wrefs.set_data(self.goodrefs)
self.wbadrefs.set_data(self.badrefs)
if len(self.goodrefs) > 0: self.wrefs.show()
if len(self.badrefs) > 0: self.wbadrefs.show()
def __addBox(self, i, box):
"""takes the number of the box in self.boxes and the (x,y,mode) tuple and displays it"""
# Display the actual box
boxsize = self.vbbsize.getValue()
ptclsize = self.vbbpsize.getValue()
try:
color = self.boxcolors[box[2]]
except:
color = self.boxcolors["unknown"]
self.wimage.add_shape(
"box{}".format(i),
EMShape(("rect", color[0], color[1], color[2],
box[0] - boxsize // 2, box[1] - boxsize // 2,
box[0] + boxsize // 2, box[1] + boxsize // 2, 2)))
self.wimage.add_shape(
"cir{}".format(i),
EMShape(("circle", color[0], color[1], color[2], box[0], box[1],
ptclsize / 2, 1.5)))
def listKey(self, event):
pass
#if event.key()>=Qt.Key_0 and event.key()<=Qt.Key_9 :
#q=int(event.key())-Qt.Key_0
#self.squality.setValue(q)
#elif event.key() == Qt.Key_Left:
#self.sdefocus.setValue(self.sdefocus.getValue()-0.03)
#elif event.key() == Qt.Key_Right:
#self.sdefocus.setValue(self.sdefocus.getValue()+0.03)
#elif event.key()==Qt.Key_I :
#self.doImport()
#elif event.key()==Qt.Key_U :
#self.unImport()
def doAutoBox(self, b):
"""Autobox button pressed, find the right algorithm and call it"""
name, bname, fn1 = self.aboxmodes[self.autotab.currentIndex()]
print name, " called"
def closeEvent(self, event):
# QtGui.QWidget.closeEvent(self,event)
E2saveappwin("e2boxer21", "main", self)
E2saveappwin("e2boxer21", "image", self.wimage.qt_parent)
E2saveappwin("e2boxer21", "particles", self.wparticles.qt_parent)
E2saveappwin("e2boxer21", "refs", self.wrefs.qt_parent)
E2saveappwin("e2boxer21", "badrefs", self.wbadrefs.qt_parent)
#self.writeCurParm()
event.accept()
QtGui.qApp.exit(0)