def write(self, name, values): value_to_str = self._value_to_str_func(self.dtype) FileWriter.write(self, name) for value in values: FileWriter.write(self, "\t") FileWriter.write(self, value_to_str(value)) FileWriter.write(self, "\n")
def write_header(self, header = None):
if isinstance(header, MatrixHeader):
FileWriter.write(self, "\t".join(header.columns))
elif isinstance(header, (list, tuple)):
FileWriter.write(self, "\t".join(header))
else:
raise Exception("Unsupported headers type: {}".format(str(type(header))))
FileWriter.write(self, "\n")
def merge(log, input, output, gitools_output):
"""
Merge repeated rows by the lowest pvalue, in case the pvalue is the same take the one with greater n
"""
f = FileReader(input)
hdr = f.readline().rstrip().split("\t")
upreg = {}
downreg = {}
upreg_count = 0
downreg_count = 0
mid_index = 8
for line in f:
line = line.rstrip()
if len(line) == 0:
continue
fields = line.split("\t")
row_name = fields[0]
upreg_count += merge_data(row_name, fields[1:mid_index], upreg)
downreg_count += merge_data(row_name, fields[mid_index:], downreg)
f.close()
upreg_keys = upreg.keys()
downreg_keys = downreg.keys()
log.debug("Total rows: upreg = {}, downreg = {}".format(len(upreg_keys), len(downreg_keys)))
log.debug("Merged rows: upreg = {}, downreg = {}".format(upreg_count, downreg_count))
ofile = FileWriter(output)
ofile.write("\t".join(hdr))
ofile.write("\n")
gfile = FileWriter(gitools_output)
gfile.write("column\trow\t")
gfile.write("\t".join([x[6:] for x in hdr if x.startswith("upreg_")]))
gfile.write("\n")
for row_name in upreg_keys:
upreg_data = upreg[row_name]
upreg_data_join = "\t".join(upreg_data)
downreg_data = downreg[row_name]
downreg_data_join = "\t".join(downreg_data)
ofile.write(row_name)
ofile.write("\t")
ofile.write(upreg_data_join)
ofile.write("\t")
ofile.write(downreg_data_join)
ofile.write("\n")
gfile.write("upreg\t")
gfile.write(row_name)
gfile.write("\t")
gfile.write(upreg_data_join)
gfile.write("\n")
gfile.write("downreg\t")
gfile.write(row_name)
gfile.write("\t")
gfile.write(downreg_data_join)
gfile.write("\n")
ofile.close()
gfile.close()
return (upreg_count, downreg_count)
def write_data_map(dmap, path):
rf = FileWriter(path)
hdr = ["id"]
hdr.extend(["_".join(("gain", f.replace("-", "_").lower())) for f in FIELDS])
hdr.extend(["_".join(("loss", f.replace("-", "_").lower())) for f in FIELDS])
rf.write("\t".join(hdr) + "\n")
for row, values in dmap.iteritems():
rf.write(row)
for v in values:
rf.write("\t")
rf.write(v)
if len(values) == len(FIELDS):
rf.write("\t".join(["-"] * len(FIELDS)))
rf.write("\n")
rf.close()
def combination(log, conf, rs, c, data_repo, results_path, conditions):
cid = c["id"]
ids = c["source/ids"]
files = c["files"]
results_url = data_repo.url(results_path)
try:
# prepare temporary path and files
tmp_path = mkdtemp(prefix = "cnv_combination_")
data_file = os.path.join(tmp_path, "data.tdm")
columns_file = os.path.join(tmp_path, "columns.gmt")
tmp_file = os.path.join(tmp_path, "tmp.tdm")
log.debug("Temporary directory: {}".format(tmp_path))
# join files to combine in a single TDM file
log.info("Joining files ...".format(files[0]))
outpf = FileWriter(data_file)
log.debug("\t{} ...".format(files[0]))
repo, path = rs.from_url(files[0])
local_path = repo.get_local(path)
ref_hdr = tdm.unflatten(local_path, outpf, row_column = "id",
column_and_attr_func = lambda name: unflatten_filtered_names(name, ids[0]))
#outpf.flush()
#ref_hdr = tdm.read_header_names(data_file)
repo.close_local(path)
for i in xrange(1, len(files)):
log.debug("\t{} ...".format(files[i]))
repo, path = rs.from_url(files[i])
local_path = repo.get_local(path)
hdr = tdm.unflatten(local_path, tmp_file, row_column = "id",
column_and_attr_func = lambda name: unflatten_filtered_names(name, ids[i]))
tdm.append(outpf, tmp_file, ref_hdr)
repo.close_local(path)
outpf.close()
# prepare conditions columns file in GMT format
outpf = FileWriter(columns_file)
for cond in conditions:
outpf.write(cond)
outpf.write("\t\t")
outpf.write("\t".join(["_".join((sid, cond)) for sid in ids]))
outpf.write("\n")
outpf.close()
# run gitools-combination with data.tdm
log.info("Running gitools combination ...")
log.debug("\tData: {}".format(data_file))
log.debug("\tColumns: {}".format(columns_file))
gitools_combination_bin = os.path.join(conf["bin_paths.gitools"], "bin", "gitools-combination")
cmd = " ".join([ gitools_combination_bin,
"-N", cid, "-w", tmp_path,
"-d", data_file,
"-c", columns_file,
"-pn", P_VALUE_FIELD,
"-sn n",
"-p 1", "-debug"])
log.debug(cmd)
retcode = subprocess.call(args = cmd, shell = True)
sys.stdout.write("\n")
sys.stdout.flush()
if retcode != 0:
raise Exception("Combination exit code = {}".format(retcode))
# flatten results
log.info("Flattening results into {} ...".format(results_url))
try:
results_local_path = data_repo.create_local(results_path)
tdm.flatten(os.path.join(tmp_path, cid + "-results.tdm.gz"), results_local_path,
None, ["N", "z-score", "p-value"])
data_repo.put_local(results_local_path)
except:
data_repo.close_local(results_local_path)
finally:
shutil.rmtree(tmp_path)
