def post(self):
pool_name = self.get_argument('pool_name')
pools_info = json_decode(self.get_argument('pools_info'))
concentrations = []
input_compositions = []
for p_info in pools_info:
pool_comp = PoolComposition(p_info['pool_id'])
concentrations.append({
'composition': pool_comp,
'concentration': p_info['concentration']
})
input_compositions.append({
'composition':
pool_comp,
'input_volume':
p_info['volume'],
'percentage_of_output':
p_info['percentage']
})
# Create the quantification process (DNA conc)
q_process = QuantificationProcess.create_manual(
self.current_user, concentrations)
# Create the pool - Magic number 5 - > the volume for this pooling
# is always 5 according to the wet lab.
p_process = PoolingProcess.create(self.current_user, q_process,
pool_name, 5, input_compositions, {
"function": "amplicon_pool",
"parameters": {}
})
self.write({'process': p_process.id})
def create_pool_quantification_process(user, pools):
concentrations = np.around(np.random.rand(len(pools)), 6)
concentrations = [{
'composition': p,
'concentration': c
} for p, c in zip(pools, concentrations)]
return QuantificationProcess.create_manual(user, concentrations)