ttk.Radiobutton(panel, text='Integrate', var=diff_check, value='integrate', style='White.TRadiobutton', command=lambda: differentiate()).place(relx=0.05, y=180) ttk.Checkbutton(panel, text='Detrend', style='White.TCheckbutton', var=trend, command=lambda: detrend()).place(relx=0.05, y=200) ttk.Checkbutton(panel, text='Detrending line', style='White.TCheckbutton', var=trend_line, command=lambda: detrend()).place(relx=0.4, y=200) toolbar = Navigator(canvas, root, filter_type_cbb, [ left_cutoff_entry, right_cutoff_entry, filter_order, sampling_frequency, filter_type_cbb ], [can_click, 'button_press_event', click], [can_enter, 'axes_enter_event', hover_enter], [can_leave, 'axes_leave_event', hover_leave]) toolbar.place(relx=0.1, rely=0, anchor="nw") toolbar.config(background='white') toolbar._message_label.config(background='white') differentiate('save') detrend() root.mainloop() # call the main window to run