def test_rmdup_clipped_unpaired(self):
test_case_data = [
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t82\t20\t6181935\t60\t5S96M\t*\t0\t0\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t?<?AADADBFBF<EHIGHGGGEAF3AF<CHGGDG9?GHFFACDHH)?@AHEHHIIIIE>A=A:?);B27@;@?>,;;C(5::>>>@5:()4>@@@######\tXC:i:96",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t82\t20\t6181930\t60\t101M\t*\t0\t0\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA",
]
self.__reducer.discard_duplicates = False
sams = map(SAMMapping, test_case_data)
left_key = "0020:000006181930:R"
pairs = ( (sams[0], None), (sams[1], None) )
# add the pairs to the context
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[0]))
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[1]))
self.__reducer.reduce(self.__ctx)
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([1,1], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST200R_251:6:2207:18561:163438#GCCAAT"
good_read_sam = self.__ctx.emitted[key][0]
mapping = SAMMapping("\t".join( (key,good_read_sam) ))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST200R_251:5:1208:19924:124635#GCCAAT"
dup_read_sam = self.__ctx.emitted[key][0]
mapping = SAMMapping("\t".join( (key,dup_read_sam) ))
self.assertTrue(mapping.is_duplicate())
def test_rmdup_bug(self):
test_case_data = [
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t83\t20\t6181935\t60\t5S96M\t=\t6181919\t-112\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t?<?AADADBFBF<EHIGHGGGEAF3AF<CHGGDG9?GHFFACDHH)?@AHEHHIIIIE>A=A:?);B27@;@?>,;;C(5::>>>@5:()4>@@@######\tXC:i:96\tXT:A:U\tNM:i:1\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:1\tXO:i:0\tXG:i:0\tMD:Z:13G82\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:######@@@>4)(:5@>>>::5(C;;,>?@;@72B;)?:A=A>EIIIIHHEHA@?)HHDCAFFHG?9GDGGHC<FA3FAEGGGHGIHE<FBFBDADAA?<?",
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t163\t20\t6181919\t60\t101M\t=\t6181935\t112\tCTGAGCACACCAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACACCTCTACTGTTACCAAATCCACTCAGATTCCCAA\t@@@FFFDDFHG??;EEH>HHGIGHEGCGEGGIGJG31?DDBBD>FGG@HG??DFBBADFAGII3@EH;;CEHECBB7?>CE.;...5>ACDDA:C:;>:>?\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:29G36C34\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:@@@FFFDDFHG??;EEH>HHGIGHEGCGEGGIGJG31?DDBBD>FGG@HG??DFBBADFAGII3@EH;;CEHECBB7?>CE.;...5>ACDDA:C:;>:>?",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t83\t20\t6181938\t60\t8S93M\t=\t6181919\t-112\tAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACCCCTCTACTGTTACCAAATCCACTCAGATTCCCAAATCTTCCACTT\t@@@DDFDFHHHHHJJJEHGGHIHHAEGHJJIJJFGGHGIDIGIJJ?BBGGGIIIJJIJGFHGIJEC(=3?C;?B9?@C>CECECAA(;;3>C#########\tXC:i:93\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:10G36C45\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:#########C>3;;(AACECEC>C@?9B?;C?3=(CEJIGHFGJIJJIIIGGGBB?JJIGIDIGHGGFJJIJJHGEAHHIHGGHEJJJHHHHHFDFDD@@@",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t163\t20\t6181919\t60\t101M\t=\t6181938\t112\tCTGAGCACACCAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACACCTCTACTGTTACCAAATCCACTCAGATTCCCAA\t@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:29G36C34\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA",
]
self.__reducer.discard_duplicates = False
sams = map(SAMMapping, test_case_data)
left_key = "0020:000006181919:F"
pairs = ( (sams[1], sams[0]), (sams[3], sams[2]) )
# add the pairs to the context
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[0]))
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[1]))
self.__reducer.reduce(self.__ctx)
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([2,2], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST200R_251:6:2207:18561:163438#GCCAAT"
good_pair_sam = self.__ctx.emitted[key]
for read in good_pair_sam:
mapping = SAMMapping("\t".join( (key,read) ))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST200R_251:5:1208:19924:124635#GCCAAT"
dup_pair_sam = self.__ctx.emitted[key]
for read in dup_pair_sam:
mapping = SAMMapping("\t".join( (key,read) ))
self.assertTrue(mapping.is_duplicate())
def test_rmdup_clipped_unpaired(self):
test_case_data = [
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t82\t20\t6181935\t60\t5S96M\t*\t0\t0\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t?<?AADADBFBF<EHIGHGGGEAF3AF<CHGGDG9?GHFFACDHH)?@AHEHHIIIIE>A=A:?);B27@;@?>,;;C(5::>>>@5:()4>@@@######\tXC:i:96",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t82\t20\t6181930\t60\t101M\t*\t0\t0\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA",
]
self.__reducer.discard_duplicates = False
sams = map(SAMMapping, test_case_data)
left_key = "0020:000006181930:R"
pairs = ((sams[0], None), (sams[1], None))
# add the pairs to the context
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[0]))
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[1]))
self.__reducer.reduce(self.__ctx)
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([1, 1], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST200R_251:6:2207:18561:163438#GCCAAT"
good_read_sam = self.__ctx.emitted[key][0]
mapping = SAMMapping("\t".join((key, good_read_sam)))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST200R_251:5:1208:19924:124635#GCCAAT"
dup_read_sam = self.__ctx.emitted[key][0]
mapping = SAMMapping("\t".join((key, dup_read_sam)))
self.assertTrue(mapping.is_duplicate())
def test_rmdup_bug(self):
test_case_data = [
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t83\t20\t6181935\t60\t5S96M\t=\t6181919\t-112\tAAGTGGAAGATTTGGGAATCTGAGTGGATTTGGTAACAGTAGAGGGGTGGATCTGGCTTGGAAAACAATCGAGGTACCAATATAGGTGGTAGATGAATTTT\t?<?AADADBFBF<EHIGHGGGEAF3AF<CHGGDG9?GHFFACDHH)?@AHEHHIIIIE>A=A:?);B27@;@?>,;;C(5::>>>@5:()4>@@@######\tXC:i:96\tXT:A:U\tNM:i:1\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:1\tXO:i:0\tXG:i:0\tMD:Z:13G82\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:######@@@>4)(:5@>>>::5(C;;,>?@;@72B;)?:A=A>EIIIIHHEHA@?)HHDCAFFHG?9GDGGHC<FA3FAEGGGHGIHE<FBFBDADAA?<?",
"HWI-ST200R_251:5:1208:19924:124635#GCCAAT\t163\t20\t6181919\t60\t101M\t=\t6181935\t112\tCTGAGCACACCAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACACCTCTACTGTTACCAAATCCACTCAGATTCCCAA\t@@@FFFDDFHG??;EEH>HHGIGHEGCGEGGIGJG31?DDBBD>FGG@HG??DFBBADFAGII3@EH;;CEHECBB7?>CE.;...5>ACDDA:C:;>:>?\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:29G36C34\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:@@@FFFDDFHG??;EEH>HHGIGHEGCGEGGIGJG31?DDBBD>FGG@HG??DFBBADFAGII3@EH;;CEHECBB7?>CE.;...5>ACDDA:C:;>:>?",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t83\t20\t6181938\t60\t8S93M\t=\t6181919\t-112\tAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACCCCTCTACTGTTACCAAATCCACTCAGATTCCCAAATCTTCCACTT\t@@@DDFDFHHHHHJJJEHGGHIHHAEGHJJIJJFGGHGIDIGIJJ?BBGGGIIIJJIJGFHGIJEC(=3?C;?B9?@C>CECECAA(;;3>C#########\tXC:i:93\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:10G36C45\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:#########C>3;;(AACECEC>C@?9B?;C?3=(CEJIGHFGJIJJIIIGGGBB?JJIGIDIGHGGFJJIJJHGEAHHIHGGHEJJJHHHHHFDFDD@@@",
"HWI-ST200R_251:6:2207:18561:163438#GCCAAT\t163\t20\t6181919\t60\t101M\t=\t6181938\t112\tCTGAGCACACCAAAATTCATCTACCACCTATATTGGTACCTCGATTGTTTTCCAAGCCAGATCCACACCTCTACTGTTACCAAATCCACTCAGATTCCCAA\t@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA\tXT:A:U\tNM:i:2\tSM:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tAM:i:37\tX0:i:1\tX1:i:0\tXM:i:2\tXO:i:0\tXG:i:0\tMD:Z:29G36C34\tRG:Z:raw_merged-1.2.3.4.5.6.7.8.bam\tOQ:Z:@CCFFDDFHHHHHIJJJIIJJJIJJIIJGJIIIJII?DGHIGHDGHIIIJIJIJIIDCHGIJIIGGHIFEHHHHFFFFFDC.6.66;@CCCDCCDC>CCCA",
]
self.__reducer.discard_duplicates = False
sams = map(SAMMapping, test_case_data)
left_key = "0020:000006181919:F"
pairs = ((sams[1], sams[0]), (sams[3], sams[2]))
# add the pairs to the context
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[0]))
self.__ctx.add_value(left_key, proto.serialize_pair(pairs[1]))
self.__reducer.reduce(self.__ctx)
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([2, 2], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST200R_251:6:2207:18561:163438#GCCAAT"
good_pair_sam = self.__ctx.emitted[key]
for read in good_pair_sam:
mapping = SAMMapping("\t".join((key, read)))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST200R_251:5:1208:19924:124635#GCCAAT"
dup_pair_sam = self.__ctx.emitted[key]
for read in dup_pair_sam:
mapping = SAMMapping("\t".join((key, read)))
self.assertTrue(mapping.is_duplicate())
def test_fw_rev_missed_dup_pair(self):
"""
Here we have two duplicate pairs, as detected by Picard.
The reads 10364 have flags 'pPR1', 'pPr2'.
The reads 138222 have flags 'pPR2d', 'pPr1d'.
10364/1 has no trimming and is on the fw strand. It should have a key position 88888399:F.
10364/2 is also on the rev strand. We expect a key position of 88888421+53 = 88888474:R.
138222/1 is on the rev strand. We expect a key position of 88888404+70 = 88888474:R.
138222/2 has been trimmed but is on the fw strand. It should have a key position 88888399:F.
"""
test_case_data = [
"HWI-ST332_97:3:66:16214:10364#0\t99\t10\t88888399\t46\t76M\t=\t88888421\t76\tTGATTTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\tHGHHHHHHFHEHHHHGHHFHHHGGHHHHHHHHHHEHDHGEHFHHHHHHHGGHHHHHHHHHHBHBBEGG=GFFFF@F",
"HWI-ST332_97:3:66:16214:10364#0\t147\t10\t88888421\t46\t22S54M\t=\t88888399\t-76\tTGATTCCGCTCCATGTGCCTCGAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\t#######################AA@EHGHEHHHHHHHHHHHHHHHEHHHHHHFHFHHHHHHHHHHHHHHHGHHFH",
"HWI-ST332_97:3:7:10556:138222#0\t1107\t10\t88888404\t23\t5S71M\t=\t88888399\t-76\tTGATGTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\t######C<BCC:B,DDDC=BD3@CB8B?DBD@E@EEEEECED@CDB=8C7A@D=DEDEDCDBECDE<>;;,17,45",
"HWI-ST332_97:3:7:10556:138222#0\t1187\t10\t88888399\t15\t50M26S\t=\t88888404\t76\tTGATTTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCCGTCTGGTTGCTTCTATTTTG\tCC7EEFFF@FHHFHHG?GGF:>4.7GD8DC@D>CCFGG@GGG5GG4<CB###########################",
]
sams = map(SAMMapping, test_case_data)
pair1 = sams[0:2]
pair2 = sams[2:]
left_key = "0010:000088888399:F"
self.__ctx.add_value(left_key, proto.serialize_pair(pair1))
self.__ctx.add_value(left_key, proto.serialize_pair(pair2))
self.__reducer.reduce(self.__ctx)
# verify emitted data. 2 keys (one per read name) and 2 reads for each key
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([2,2], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST332_97:3:66:16214:10364#0"
good_pair_sam = self.__ctx.emitted[key]
for read in good_pair_sam:
mapping = SAMMapping("\t".join( (key,read) ))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST332_97:3:7:10556:138222#0"
dup_pair_sam = self.__ctx.emitted[key]
for read in dup_pair_sam:
mapping = SAMMapping("\t".join( (key,read) ))
self.assertTrue(mapping.is_duplicate())
self.setUp() # clean-up and repeat for the right key
right_key = "0010:000088888474:R"
self.__ctx.add_value(right_key, PAIR_STRING)
self.__ctx.add_value(right_key, PAIR_STRING)
self.__reducer.reduce(self.__ctx)
# verify no data emitted
self.assertEqual(0, len(self.__ctx.emitted))
def test_fw_rev_missed_dup_pair(self):
"""
Here we have two duplicate pairs, as detected by Picard.
The reads 10364 have flags 'pPR1', 'pPr2'.
The reads 138222 have flags 'pPR2d', 'pPr1d'.
10364/1 has no trimming and is on the fw strand. It should have a key position 88888399:F.
10364/2 is also on the rev strand. We expect a key position of 88888421+53 = 88888474:R.
138222/1 is on the rev strand. We expect a key position of 88888404+70 = 88888474:R.
138222/2 has been trimmed but is on the fw strand. It should have a key position 88888399:F.
"""
test_case_data = [
"HWI-ST332_97:3:66:16214:10364#0\t99\t10\t88888399\t46\t76M\t=\t88888421\t76\tTGATTTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\tHGHHHHHHFHEHHHHGHHFHHHGGHHHHHHHHHHEHDHGEHFHHHHHHHGGHHHHHHHHHHBHBBEGG=GFFFF@F",
"HWI-ST332_97:3:66:16214:10364#0\t147\t10\t88888421\t46\t22S54M\t=\t88888399\t-76\tTGATTCCGCTCCATGTGCCTCGAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\t#######################AA@EHGHEHHHHHHHHHHHHHHHEHHHHHHFHFHHHHHHHHHHHHHHHGHHFH",
"HWI-ST332_97:3:7:10556:138222#0\t1107\t10\t88888404\t23\t5S71M\t=\t88888399\t-76\tTGATGTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCATTCTGGAAGCTTATCTATTG\t######C<BCC:B,DDDC=BD3@CB8B?DBD@E@EEEEECED@CDB=8C7A@D=DEDEDCDBECDE<>;;,17,45",
"HWI-ST332_97:3:7:10556:138222#0\t1187\t10\t88888399\t15\t50M26S\t=\t88888404\t76\tTGATTTTGCTCCATTGTCTTCTAGCTTGTGTTATGCCTGTTGAAAGTACAAAATCCGTCTGGTTGCTTCTATTTTG\tCC7EEFFF@FHHFHHG?GGF:>4.7GD8DC@D>CCFGG@GGG5GG4<CB###########################",
]
sams = map(SAMMapping, test_case_data)
pair1 = sams[0:2]
pair2 = sams[2:]
left_key = "0010:000088888399:F"
self.__ctx.add_value(left_key, proto.serialize_pair(pair1))
self.__ctx.add_value(left_key, proto.serialize_pair(pair2))
self.__reducer.reduce(self.__ctx)
# verify emitted data. 2 keys (one per read name) and 2 reads for each key
self.assertEqual(2, len(self.__ctx.emitted))
self.assertEqual([2, 2], map(len, self.__ctx.emitted.itervalues()))
key = "HWI-ST332_97:3:66:16214:10364#0"
good_pair_sam = self.__ctx.emitted[key]
for read in good_pair_sam:
mapping = SAMMapping("\t".join((key, read)))
self.assertFalse(mapping.is_duplicate())
key = "HWI-ST332_97:3:7:10556:138222#0"
dup_pair_sam = self.__ctx.emitted[key]
for read in dup_pair_sam:
mapping = SAMMapping("\t".join((key, read)))
self.assertTrue(mapping.is_duplicate())
self.setUp() # clean-up and repeat for the right key
right_key = "0010:000088888474:R"
self.__ctx.add_value(right_key, PAIR_STRING)
self.__ctx.add_value(right_key, PAIR_STRING)
self.__reducer.reduce(self.__ctx)
# verify no data emitted
self.assertEqual(0, len(self.__ctx.emitted))