def scan_through_train_expt(params_expt_input, train_increment, num_stims):
len_peak_region_in_samples = round(
(params_expt_input[3] - params_expt_input[2]) /
stf.get_sampling_interval())
expt_peaks = np.zeros((stf.get_size_channel(), num_stims))
expt_peak_arrays = np.zeros(
(stf.get_size_channel(), num_stims, len_peak_region_in_samples))
trace = 0
while trace < stf.get_size_channel():
params_expt = params_expt_input
[expt_peaks[trace], expt_peak_arrays[trace]
] = scan_through_train(params_expt, train_increment, num_stims, trace)
params_expt[2] = params_expt_input[2] - (train_increment * (num_stims))
params_expt[3] = params_expt_input[3] - (train_increment * (num_stims))
trace += 1
loaded_file = stf.get_filename()[:-3]
np.savetxt(loaded_file + '_peaks.csv',
expt_peaks,
delimiter=',',
newline='\n')
return (expt_peaks, expt_peak_arrays)
def find_AP_peak_ADP(start_msec, delta_msec, current_start, current_delta, threshold_value, deflection_direction, mark_option):
""" count number of APs in traces with current injection/gradually increasing steps
inputs: (time (msec) to start search, length of search region, starting current value, current delta between traces, threshold value, deflection direction ('up'/'down'), mark traces (True/False))"""
loaded_file = stf.get_filename()[:-4]
event_counts = np.zeros((stf.get_size_channel(),2));
for trace_ in range(stf.get_size_channel()):
##gets AP counts and sample points in current trace
if deflection_direction == 'up':
direction_input = True
else:
direction_input = False
[trace_count, sample_points, time_points] = jjm_count(start_msec, delta_msec, threshold=threshold_value, up=direction_input, trace=trace_, mark=mark_option);
##gets ADP values
values, indicies = find_ADPs(sample_points)
print(values)
out_array = np.array([values, indicies])
np.savetxt(loaded_file + 'trace' + str(str(trace_).zfill(3)) +'ADP_values.csv', out_array, delimiter=',', newline='\n')
event_counts[trace_][1] = trace_count
event_counts[trace_][0] = current_start + (current_delta*trace_) ;
np.savetxt(loaded_file + '_AP_counts.csv', event_counts, delimiter=',', newline='\n');
return(event_counts)
def analyze_file(baseline_start, baseline_end, cap_trans_start, cap_trans_end,
amplitude, EPSC1_s, EPSC1_e, EPSC2_s, EPSC2_e, sweep_start,
sweep_end):
"""inputs: (baseline_start, baseline_end, cap_trans_start, cap_trans_end, amplitude, EPSC1_s, EPSC1_e, EPSC2_s, EPSC2_e, sweep_start, sweep_end)
output: numpy array where 1st column is capacitance transient amplitude, 2nd is series resistance, 3rd is 1st EPSC, 4th is 2nd EPCSC
also writes output to .csv file"""
num_sweeps = stf.get_size_channel()
print('there are')
print(num_sweeps)
print('sweeps in recording')
print('analyzing sweeps')
print(sweep_start)
print('to')
print(sweep_end)
sweeps_to_analyze = sweep_end - sweep_start
#create array for results
data_array = np.zeros((sweeps_to_analyze + 1, 4))
y = 0
for x in range(sweep_start - 1, sweep_end):
#moves to next trace
stf.set_trace(x)
[cap_trans_amplitude,
series_resistance] = jjm_resistance(baseline_start, baseline_end,
cap_trans_start, cap_trans_end,
amplitude)
data_array[y][0] = cap_trans_amplitude
data_array[y][1] = series_resistance
EPSC_1 = jjm_peak(baseline_start, baseline_end, EPSC1_s, EPSC1_e)
data_array[y][2] = EPSC_1
EPSC_2 = jjm_peak(baseline_start, baseline_end, EPSC2_s, EPSC2_e)
data_array[y][3] = EPSC_2
pp_40 = float(float(EPSC_2) / float(EPSC_1))
y += 1
#print first few entries to check accuracy
print(data_array[:3])
#make csv file with data
file_name = stf.get_filename()
#expt = file_name[-12:].rstrip('.abf');
np.savetxt(file_name + '_stimfitanalysis.csv',
data_array,
delimiter=',',
newline='\n')
return (data_array)
def find_AP_peaks(start_msec, delta_msec, current_start, current_delta, threshold_value, deflection_direction, mark_option):
""" count number of APs in traces with current injection/gradually increasing steps
inputs: (time (msec) to start search, length of search region, starting current value, current delta between traces, threshold value, deflection direction ('up'/'down'), mark traces (True/False))"""
event_counts = np.zeros((stf.get_size_channel(),2));
for trace_ in range(stf.get_size_channel()):
event_counts[trace_][1] = spells.count_events(start_msec, delta_msec, threshold=threshold_value, up=deflection_direction, trace=trace_, mark=mark_option);
event_counts[trace_][0] = current_start + (current_delta*trace_) ;
loaded_file = stf.get_filename()[:-4] ;
np.savetxt(loaded_file + '_AP_counts.csv', event_counts, delimiter=',', newline='\n');
return(event_counts)
def batch_compile(file_list, summary_file_name):
means_dict = {}
for fname in file_list:
stf.file_open(fname)
file_df = compile_amplitudes_in_trace()
means_dict[stf.get_filename()] = file_df.mean(axis=0)
summary_df = pd.DataFrame(means_dict)
summary_df.to_excel(str(summary_file_name) + '_amplitudes_compiled.xlsx')
return (summary_df)
def find_ADP_thresholds_for_file(current_start_file, current_delta_file,
*argv):
""" count number of APs in traces with current injection/gradually increasing steps
inputs: (time (msec) to start search, length of search region, starting current value, current delta between traces, threshold value, deflection direction ('up'/'down'), mark traces (True/False))"""
if len(argv) > 0:
threshold_value_file = argv[0]
deflection_direction_file = argv[1]
mark_option_file = argv[2]
start_msec_file = float(argv[3])
delta_msec_file = float(argv[4])
else:
threshold_value_file = 0
deflection_direction_file = 'up'
mark_option_file = True
start_msec_file = float(stf.get_peak_start(True))
delta_msec_file = float(stf.get_peak_end(True) - start_msec_file)
loaded_file = stf.get_filename()[:-4]
event_counts = np.zeros((stf.get_size_channel(), 2))
ADPs = np.zeros((stf.get_size_channel(), 2))
thresholds = np.zeros((stf.get_size_channel(), 2))
trace_df_dict = {}
for trace_ in range(stf.get_size_channel()):
AP_count_for_trace, df_for_trace = find_AP_peak_ADP_trace(
trace_, threshold_value_file, deflection_direction_file,
mark_option_file, start_msec_file, delta_msec_file)
trace_df_dict['trace' + str(str(trace_).zfill(3))] = df_for_trace
event_counts[trace_][1] = AP_count_for_trace
event_counts[trace_][0] = current_start_file + (current_delta_file *
trace_)
np.savetxt(loaded_file + '_AP_counts.csv',
event_counts,
delimiter=',',
newline='\n')
output_path = loaded_file + 'ADP_thresholds.xlsx'
xlsx_out = pd.ExcelWriter(output_path, engine='xlsxwriter')
for trace_name, trace_df in sorted(trace_df_dict.items()):
trace_df.to_excel(xlsx_out, sheet_name=trace_name)
xlsx_out.save()
return (True)
def compile_amplitudes_in_trace():
# for each trace in file run find_baseline_amplitudes
output_array = np.array(['baseline', 'peak', 'peak_from_baseline'])
for trace in range(stf.get_size_channel()):
stf.set_trace(trace)
fba_output = find_baseline_amplitude(10)
output_array = np.vstack([output_array, fba_output])
output_df = pd.DataFrame(output_array[1:],
columns=output_array[0],
dtype=float)
output_df.to_excel(str(stf.get_filename()[-40:-3]) + '.xlsx')
return (output_df)
perform events detection as decribed in the Stimfit manual [1]
It creates a preliminary and final templates from a file 'minis.dat'.
You can download the file here: http://stimfit.org/tutorial/minis.dat
last revision: Wed Sep 5 09:38:41 CEST 2018
C. Schmidt-Hieber
[1] https://neurodroid.github.io/stimfit/manual/event_extraction.html
"""
import stf
from wx import MessageBox
if stf.get_filename()[-9:] != 'minis.dat':
MessageBox('Use minis.dat for this demo.', 'Warning')
def preliminary():
"""
Sets peak, base and fit cursors around a synaptic event
and performs a biexponential fit to create the preliminary template
for event detection.
"""
stf.base.cursor_index = (209600, 209900)
stf.peak.cursor_index = (209900, 210500)
stf.fit.cursor_index = (209900, 210400)
stf.set_peak_mean(3)
