def pca_projected_clusters(feature_matrix):
n_components = 5
if not util.is_server():
color_names = colors.cnames
df = pd.read_csv('{}/pca_projected_clusters.csv'.format(util.get_output_dir()))
df = df[['x', 'y', 'label']]
ax = df[df['label'] == 0].plot(kind='scatter', x='x', y='y', c=random.choice(list(color_names.keys())),
label='Phenotype 0')
for k in range(1, n_components):
cluster = df[df['label'] == k]
if cluster.shape[0] > 0:
cluster.plot(kind='scatter', x='x', y='y', c=random.choice(list(color_names.keys())),
label='Phenotype {}'.format(k), ax=ax)
plt.title('PCA Projected Clusters')
plt.savefig('{}/pca_projected_clusters.png'.format(util.get_output_dir()))
plt.clf()
return
W, H, _ = clustering.get_nmf_w_h(feature_matrix, n_components=n_components, alpha=5, l1_ratio=1.0)
labels = [label for eid, label in clustering.get_cluster_assignments(np.zeros(W.shape[0]), W)]
model = TruncatedSVD(n_components=2)
data_r = model.fit_transform(feature_matrix)
d = {}
d['x'] = [data[0] for data in data_r]
d['y'] = [data[1] for data in data_r]
d['label'] = labels
df = pd.DataFrame(d)
df.to_csv('{}/pca_projected_clusters.csv'.format(util.get_output_dir()))
def hyperparameter_tuning(feature_matrix):
alphas = [1, 5, 15]
l1_ratios = [0, .5, 1]
ks = range(2, 40, 5)
threshold = 1
d = {}
for k in ks:
for alpha in alphas:
for l1_ratio in l1_ratios:
W, H, _ = get_nmf_w_h(feature_matrix, n_components=k, alpha=alpha, l1_ratio=l1_ratio)
avg_count_above_threshold = np.mean(get_count_features_above_threshold(H, threshold))
num_labels = len(set([label for _, label in get_cluster_assignments(np.zeros(W.shape[0]), W)]))
d['k'] = d.get('k', []) + [k]
d['alpha'] = d.get('alpha', []) + [alpha]
d['l1_ratio'] = d.get('l1_ratio', []) + [l1_ratio]
d['num_labels'] = d.get('num_labels', []) + [num_labels]
d['avg_nonzero_feature_count'] = d.get('avg_nonzero_feature_count', []) + [avg_count_above_threshold]
print('k={0}, alpha={1}, l1_ratio={2}, num_labels={3}, count={4}'.format(k, alpha, l1_ratio, num_labels, avg_count_above_threshold))
#output all results
df = pd.DataFrame(d)
df.to_csv('{}/hyperparamtuning.csv'.format(util.get_output_dir()))
#output optimal results
df = df[(df['count'] >= 5) & (df['count'] <= 10)]
df.to_csv('{}/optimalparams.csv'.format(util.get_output_dir()))
def k_vs_silhouette(feature_matrix, distance_metric='euclidean'):
if not util.is_server():
df = pd.read_csv('{}/silhouette_avg.csv'.format(util.get_output_dir()))
df = df[['k', 'silhouette_avg']].set_index('k')
df.plot(title='K vs Silhouette Average')
plt.xlabel('K')
plt.ylabel('Silhouette average')
plt.show()
return
silhouette_avgs = []
ks = range(2, 40, 4)
for k in ks:
W, H, _ = clustering.get_nmf_w_h(feature_matrix, n_components=k)
labels = [label for eid, label in clustering.get_cluster_assignments(np.zeros(W.shape[0]), W)]
for iter in range(3):
avgs = []
avgs.append(metrics.silhouette_score(feature_matrix, labels, distance_metric,
sample_size=10000))
silhouette_avgs.append(sum(avgs) / len(avgs))
d = {}
d['k'] = ks
d['silhouette_avg'] = silhouette_avgs
df = pd.DataFrame(d)
df.to_csv('{}/silhouette_avg.csv'.format(util.get_output_dir()))
def vis_feature_bar(feature_matrix):
frequency = feature_matrix.sum(axis = 0)
bar_num = frequency.shape[1]
plt.bar(range(0,bar_num),frequency.getA().flatten())
plt.ylabel('Frequency')
plt.xlabel('Feature#')
plt.title('Frequency of Features')
plt.savefig('{}/frequency.png'.format(util.get_output_dir()))
plt.clf()
def vis_info_table(H, feature_names):
nonzero_feature_names = clustering.get_nonzero_featurenames(H, .1, feature_names)
feature_names_weight_df = pd.DataFrame(nonzero_feature_names).set_index('featureName')
med_info_df = pd.read_csv('{}/medicationCodes/coding4.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'coding': str},
usecols=['coding', 'meaning']).rename(columns = {'coding':'node_id'})
med_info_df['node_id'] = '20003='+ med_info_df['node_id'].astype(str)
med_info_df['category'] = 'med'
med_info = feature_names_weight_df\
.join(med_info_df.set_index('node_id'), how='inner')\
.reset_index()\
.drop('index',axis = 1)
noncan_info_df = pd.read_csv('{}/nonCancerIllnessCodes/coding6.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'node_id': str},
usecols=['node_id', 'meaning'])
noncan_info_df['node_id'] = '20002=' + noncan_info_df['node_id'].astype(str)
noncan_info_df['category'] = 'noncan'
noncan_info = feature_names_weight_df\
.join(noncan_info_df.set_index('node_id'), how='inner')\
.reset_index()\
.drop('index',axis = 1)
can_info_df = pd.read_csv('{}/cancerCodes/coding3.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'node_id': str},
usecols=['node_id', 'meaning'])
can_info_df['node_id'] = '20001=' + can_info_df['node_id'].astype(str)
can_info_df['category'] = 'cancer'
can_info = feature_names_weight_df\
.join(can_info_df.set_index('node_id'), how='inner')\
.reset_index()\
.drop('index',axis = 1)
frame = [med_info, noncan_info, can_info]
f = open('{}/info_table.csv'.format(util.get_output_dir()),'w+')
info_table = pd.concat(frame).groupby(['label','category'])\
.apply(lambda x: x.loc[:,['label','category','meaning','weight']]\
.to_csv(f,index = False))
def iteration_vs_error(feature_matrix, n_components):
iterations = range(10, 300, 10)
errors = []
for iteration in iterations:
_, _, error = clustering.get_nmf_w_h(feature_matrix, n_components, max_iter=iteration)
errors.append(error)
d = {'error': errors, 'iteration': iterations}
df = pd.DataFrame(d)
df.plot(x='iteration', y='error')
plt.xlabel('Iteration')
plt.ylabel('Error')
plt.savefig('{}/iteration_vs_error.png'.format(util.get_output_dir()))
plt.clf()
def vis_grs(labels):
grs_df = pd.read_csv('{}/GWAS_Catalog_20170317_50traits_Good_CALCULATED_GRS.txt'.format(util.get_output_dir()),sep='\t')
cluster_assignment_df = pd.DataFrame.from_records(labels)
cluster_assignment_df.columns = ['Indiv', 'cluster']
grs_cluster_df = grs_df.join(cluster_assignment_df.set_index('Indiv'), on = 'Indiv')
grs_cluster_df = grs_cluster_df[pd.notnull(grs_cluster_df['cluster'])].drop('Indiv',axis = 1)
grs_cluster_df_group = grs_cluster_df.groupby(["cluster"])
matrix = grs_cluster_df_group.mean().as_matrix()
plt.imshow(matrix, interpolation='nearest', cmap=plt.cm.ocean, aspect = 'auto')
plt.colorbar()
plt.ylabel('Phenotype')
plt.xlabel('Disease')
plt.title('Average Genetic Risk Score of Each Clutser ')
plt.savefig('{}/grs.png'.format(util.get_output_dir()))
plt.clf()
def nonzero_alpha_beta_plot(k):
hyperpara_df = pd.read_csv('{}/hyperparamtuning.csv'.format(util.get_output_dir()),
dtype={'k':int, 'alpha':float,'beta':int,'count':int},
usecols=['k', 'alpha','beta','count'])
alphas = [.01, .1, 1, 10, 100]
betas = [1, 10, 100, 1000]
hyperpara_df = hyperpara_df[hyperpara_df['k']==k]
alpha_beat_array =[]
for alpha in alphas:
temp = []
for beta in betas:
index = np.logical_and(hyperpara_df['alpha']==alpha,hyperpara_df['beta']==beta)
temp.append(hyperpara_df[index]['count'].values[0])
alpha_beat_array.append(temp)
alpha_beat_array = np.asarray(alpha_beat_array)
group_bar(alpha_beat_array,alphas,betas,'Alpha-Beta-Count Bar Plot')
def group_bar(data,x_ticklabel,legend,category):
width = 0.15
fig, ax = plt.subplots(figsize=(15,10))
pos = range(0,data.shape[0])
times = range(0,data.shape[1])
for i in times:
postion = [ p + i*width for p in pos]
plt.bar(postion,data[:,i],width,alpha=0.5)
ax.set_ylabel("Count")
ax.set_xticks([p + width*(data.shape[1]-1)/2 for p in pos ])
ax.set_xticklabels(x_ticklabel)
ax.set_title(category)
plt.legend(["cluster"+str(i) for i in pos],loc = "upper left")
plt.ylim([0, max(data[0,:])] )
if category in ['Medication','Cancer','NonCancer Illness']:
ax.set_xlabel("Threshold")
plt.legend(["cluster"+str(i) for i in legend],loc = "upper left")
else:
ax.set_xlabel('Alpha')
plt.legend(["Beta = "+str(i) for i in legend],loc = "upper left")
plt.grid()
plt.savefig('{}/'.format(util.get_output_dir())+category+'_threshold_bar.png')
plt.clf()
def load_sparse_feature_matrix():
medication_df = pd.read_csv('{}/MEDICATIONS/part-00000'.format(
get_output_dir()),
dtype={'featureName': str})
cancer_df = pd.read_csv('{}/CANCERS/part-00000'.format(get_output_dir()),
dtype={'featureName': str})
non_cancer_illness_df = pd.read_csv(
'{}/NON_CANCER_ILLNESSES/part-00000'.format(get_output_dir()),
dtype={'featureName': str})
lifestyle_df = pd.read_csv('{}/LIFESTYLE/part-00000'.format(
get_output_dir()),
dtype={'featureName': str})
demographic_df = pd.read_csv('{}/DEMOGRAPHIC/part-00000'.format(
get_output_dir()),
dtype={'featureName': str})
physical_measure_df = pd.read_csv('{}/PHYSICALMEASURE/part-00000'.format(
get_output_dir()),
dtype={'featureName': str})
df = non_cancer_illness_df \
.append(cancer_df) \
.append(medication_df) \
# # .append(physical_measure_df)\
# # .append(lifestyle_df)\
# # .append(demographic_df)\
cols = ['featureName', 'featureValue']
df = df.sort_values(by='eid')
grouped = df.groupby('eid')
l = [dict(zip(*g[cols].T.values)) for n, g in grouped]
vec = DictVectorizer()
sparse_feature_matrix = vec.fit_transform(l)
eids = [group['eid'].unique()[0] for _, group in grouped]
feature_names = vec.get_feature_names()
return eids, sparse_feature_matrix, feature_names
lp = likelihood(Y[i, j], 1)
lm = likelihood(Y[i, j], -1)
a = m + 0.5 * (lp - lm)
X[i, j] = tanh(a)
for _ in tqdm(range(T)):
for i in range(N):
for j in range(M):
optimize(i, j)
X[X < 0] = 0
X[X > 0] = 255
img_name = filename.split('/')[-1].split('.')[0]
cv2.imwrite('../output/denoise_vi_{}.jpg'.format(img_name), X)
if __name__ == '__main__':
get_output_dir('../output')
img_files = [
'../a1/1_noise.txt',
'../a1/2_noise.txt',
'../a1/3_noise.txt',
'../a1/4_noise.txt',
]
for img in img_files:
gibbs(img, 1, 1, 20)
vi(img, 1, 1, 20)
def silhouette_plot(eids, feature_matrix, distance_metric='euclidean'):
for k in range(2, 21, 4):
# Create a subplot with 1 row and 2 columns
fig, (ax1, ax2) = plt.subplots(1, 2)
fig.set_size_inches(18, 7)
# The 1st subplot is the silhouette plot
# The silhouette coefficient can range from -1, 1 but in this example all
# lie within [-0.1, 1]
ax1.set_xlim([-0.1, 1])
# The (n_clusters+1)*10 is for inserting blank space between silhouette
# plots of individual clusters, to demarcate them clearly.
ax1.set_ylim([0, feature_matrix.shape[0] + (k + 1) * 10])
W, H, _ = clustering.get_nmf_w_h(feature_matrix, n_components=k)
cluster_assignments = clustering.get_cluster_assignments(eids, W)
cluster_labels = np.array([assignment for eid, assignment in cluster_assignments])
sample_size = 30000
indices = np.random.permutation(feature_matrix.shape[0])[:sample_size]
feature_matrix_sample = feature_matrix[indices]
cluster_labels_sample = cluster_labels[indices]
sample_silhouette_values = metrics.silhouette_samples(feature_matrix_sample, cluster_labels_sample, distance_metric)
silhouette_avg = metrics.silhouette_score(feature_matrix, cluster_labels, distance_metric, sample_size=sample_size)
#project data onto 2 dimensions for visualization
model = TruncatedSVD(n_components=2)
data_r = model.fit_transform(feature_matrix)
y_lower = 10
for i in range(k):
# Aggregate the silhouette scores for samples belonging to
# cluster i, and sort them
ith_cluster_silhouette_values = \
sample_silhouette_values[cluster_labels == i]
ith_cluster_silhouette_values.sort()
size_cluster_i = ith_cluster_silhouette_values.shape[0]
y_upper = y_lower + size_cluster_i
color = cm.spectral(float(i) / k)
ax1.fill_betweenx(np.arange(y_lower, y_upper),
0, ith_cluster_silhouette_values,
facecolor=color, edgecolor=color, alpha=0.7)
# Label the silhouette plots with their cluster numbers at the middle
ax1.text(-0.05, y_lower + 0.5 * size_cluster_i, str(i))
# Compute the new y_lower for next plot
y_lower = y_upper + 10 # 10 for the 0 samples
ax1.set_title("The silhouette plot for the various clusters.")
ax1.set_xlabel("The silhouette coefficient values")
ax1.set_ylabel("Cluster label")
# The vertical line for average silhouette score of all the values
ax1.axvline(x=silhouette_avg, color="red", linestyle="--")
ax1.set_yticks([]) # Clear the yaxis labels / ticks
ax1.set_xticks([-0.1, 0, 0.2, 0.4, 0.6, 0.8, 1])
# 2nd Plot showing the actual clusters formed
colors = cm.spectral(cluster_labels.astype(float) / k)
ax2.scatter(data_r[:, 0], data_r[:, 1], marker='.', s=30, lw=0, alpha=0.7,
c=colors)
# # Labeling the clusters
# centers = H
# # Draw white circles at cluster centers
# ax2.scatter(centers[:, 0], centers[:, 1],
# marker='o', c="white", alpha=1, s=200)
#
# for i, c in enumerate(centers):
# ax2.scatter(c[0], c[1], marker='$%d$' % i, alpha=1, s=50)
ax2.set_title("The visualization of the clustered data.")
ax2.set_xlabel("Feature space for the 1st feature")
ax2.set_ylabel("Feature space for the 2nd feature")
plt.suptitle(("Silhouette analysis for NMF clustering"
"with n_clusters = %d" % k),
fontsize=14, fontweight='bold')
plt.savefig('{0}/silAnalysis_k={1}.png'.format(util.get_output_dir(), k))
plt.clf()
def percentage_feature_in_cluster_vs_cohort(mode, labels, H, feature_names, pop_size):
valid_modes = ['medication', 'non-cancer-illness', 'cancer']
if mode not in valid_modes:
raise AttributeError('mode must be one of {}'.format(', '.join(valid_modes)))
if not util.is_server():
df = pd.read_csv('{0}/{1}_percent_ftr_vs_clstr.csv'.format(util.get_output_dir(), mode))
pre = 'percentFeatureInCluster'
d1 = df.set_index(['meaning', 'label'])[pre].unstack(fill_value=0).add_prefix(pre).reset_index()
df = d1.set_index('meaning').join(df[['meaning', 'percentFeatureInPop']].set_index('meaning'), how='inner').drop_duplicates()
df.plot.bar(title='Percent Feature in Cluster Vs Pop', fontsize=8, rot=90, grid=True, stacked=True, figsize=[20, 20], colormap=plt.cm.ocean)
plt.xlabel(mode)
plt.gcf().subplots_adjust(bottom=0.5)
plt.savefig('{0}/{1}_percent_ftr_vs_clstr.png'.format(util.get_output_dir(), mode), bbox_inches='tight')
plt.clf()
return
medication_df = pd.read_csv('{}/MEDICATIONS/part-00000'.format(util.get_output_dir()), dtype={'featureName': str})
cancer_df = pd.read_csv('{}/CANCERS/part-00000'.format(util.get_output_dir()), dtype={'featureName': str})
non_cancer_illness_df = pd.read_csv('{}/NON_CANCER_ILLNESSES/part-00000'.format(util.get_output_dir()), dtype={'featureName': str})
if mode == 'medication':
df = medication_df
info_df = pd.read_csv('{}/medicationCodes/coding4.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'coding': str},
usecols=['coding', 'meaning'])
join_on = 'coding'
elif mode == 'non-cancer-illness':
df = non_cancer_illness_df
info_df = pd.read_csv('{}/nonCancerIllnessCodes/coding6.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'node_id': str},
usecols=['node_id', 'meaning'])
join_on = 'node_id'
elif mode == 'cancer':
df = cancer_df
info_df = pd.read_csv('{}/cancerCodes/coding3.tsv'.format(util.get_output_dir()),
delimiter='\t',
dtype={'node_id': str},
usecols=['node_id', 'meaning'])
join_on = 'node_id'
nonzero_feature_names = clustering.get_nonzero_featurenames(H, 1, feature_names)
feature_names_df = pd.DataFrame(nonzero_feature_names).drop('weight', axis=1)
#join data with cluster labels
cluster_labels_df = pd.DataFrame.from_records(labels, columns=['eid', 'label']).set_index('eid')
data = df.join(cluster_labels_df, on='eid', how='inner')
num_patients_in_cluster_df = pd.DataFrame(data.groupby(by='label')['eid']
.apply(lambda x: len(x.unique()))
.rename('numPatientsInCluster'))
count_feature_in_pop_df = pd.DataFrame(data.groupby(by='featureName')['eid']
.apply(lambda x: len(x.unique()))
.rename('countFeatureInPop'))
features_selected_by_H_df = data\
.set_index(['label', 'featureName'])\
.join(feature_names_df.set_index(['label', 'featureName']), how='inner')\
.reset_index()
feature_count_in_cluster_df = pd.DataFrame(features_selected_by_H_df
.groupby(by=['label', 'featureName'])['eid']
.apply(lambda x: len(x.unique()))
.rename('featureCountInCluster'))
data = features_selected_by_H_df.set_index(['label', 'featureName']).join(feature_count_in_cluster_df, how='inner').reset_index()
data = data.set_index('label').join(num_patients_in_cluster_df, how='inner').reset_index()
data = data.set_index('featureName').join(count_feature_in_pop_df, how='inner').reset_index()
#calculate count of patients with feauture in cluster / num patients in cluster
data['percentFeatureInCluster'] = data['featureCountInCluster'] / data['numPatientsInCluster'] * 100
#calculate total count of feature / total pop
data['percentFeatureInPop'] = data['countFeatureInPop'] / pop_size * 100
#get meaning names
data['coding_or_node_id'] = data['featureName'].apply(lambda str: str.split('=')[1])
data = data.set_index('coding_or_node_id').join(info_df.set_index(join_on), how='inner').reset_index()
data = data[['meaning', 'label', 'percentFeatureInCluster', 'percentFeatureInPop']].drop_duplicates()
data.to_csv('{0}/{1}_percent_ftr_vs_clstr.csv'.format(util.get_output_dir(), mode))
